Restriction map for pF1KB4699
 (Circular) (Six-base) MAPSORT of: pF1KB4699.seq  Check: 8546  from: 1  to: 4223



pF1KB4699  4223 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AccI GT'mk_AC

Cuts at:    710    1261     710 
   Size:        551    3672
 
Acc65I G'GTAC_C

Cuts at:   1239    1239 
   Size:       4223
 
AclI AA'CG_TT

Cuts at:   4063    4063 
   Size:       4223
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    627    2048    2480    2833    3495     627 
   Size:       1421     432     353     662    1355

  Fragments arranged by size: 

                1421    1355     662     432     353
 
AflIII A'CryG_T

Cuts at:    517    1575    3375    3656     517 
   Size:       1058    1800     281    1084

  Fragments arranged by size: 

                1800    1084    1058     281
 
AlwNI CAG_nnn'CTG

Cuts at:   1078    1528    2966    1078 
   Size:        450    1438    2335

  Fragments arranged by size: 

                2335    1438     450
 
ApaI G_GGCC'C

Cuts at:   1006    1006 
   Size:       4223
 
ApaLI G'TGCA_C

Cuts at:   3061    3061 
   Size:       4223
 
ApoI r'AATT_y

Cuts at:   1227    3576    3832    1227 
   Size:       2349     256    1618

  Fragments arranged by size: 

                2349    1618     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4223
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4223
 
AvaI C'yCGr_G

Cuts at:   1243    1243 
   Size:       4223
 
BamHI G'GATC_C

Cuts at:   1248    1248 
   Size:       4223
 
BanI G'GyrC_C

Cuts at:   1239    1653    1901    1936    1239 
   Size:        414     248      35    3526

  Fragments arranged by size: 

                3526     414     248      35
 
BanII G_rGCy'C

Cuts at:   1006    1237    2267    1006 
   Size:        231    1030    2962

  Fragments arranged by size: 

                2962    1030     231
 
BbeI G_GCGC'C

Cuts at:   1657    1905    1657 
   Size:        248    3975
 
BbvCI CC'TCA_GC

Cuts at:   1144    1144 
   Size:       4223
 
BciVI GTATCCnnnnn_n'

Cuts at:   2121    3177    4223    2121 
   Size:       1056    1046    2121

  Fragments arranged by size: 

                2121    1056    1046
 
BclI T'GATC_A

Cuts at:   1743    1743 
   Size:       4223
 
BglII A'GATC_T

Cuts at:    313    1738    3775     313 
   Size:       1425    2037     761

  Fragments arranged by size: 

                2037    1425     761
 
BlpI GC'TnA_GC

Cuts at:   1335    1335 
   Size:       4223
 
Bme1580I G_kGCm'C

Cuts at:    592    1006    1848    1941    3065     592 
   Size:        414     842      93    1124    1750

  Fragments arranged by size: 

                1750    1124     842     414      93
 
BmgBI CAC'GTC

Cuts at:    518     585     518 
   Size:         67    4156
 
BmrI ACTGGGnnnn_n'

Cuts at:   1435    1516    1851    3995    1435 
   Size:         81     335    2144    1663

  Fragments arranged by size: 

                2144    1663     335      81
 
BmtI G_CTAG'C

Cuts at:    841     841 
   Size:       4223
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2590    2590 
   Size:       4223
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    827     827 
   Size:       4223
 
Bpu10I CC'TnA_GC

Cuts at:   1144    1144 
   Size:       4223
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    700    1399    2278    2992    3290     700 
   Size:        699     879     714     298    1633

  Fragments arranged by size: 

                1633     879     714     699     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3927    3927 
   Size:       4223
 
BsaAI yAC'GTr

Cuts at:   1576    1576 
   Size:       4223
 
BsaBI GATnn'nnATC

Cuts at:      6    1737    3774       6 
   Size:       1731    2037     455

  Fragments arranged by size: 

                2037    1731     455
 
BsaHI Gr'CG_yC

Cuts at:   1654    1902    2604    4100    1654 
   Size:        248     702    1496    1777

  Fragments arranged by size: 

                1777    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1412    1933    2611    3022    3169    3443    1412 
   Size:        521     678     411     147     274    2192

  Fragments arranged by size: 

                2192     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    194     224     194 
   Size:         30    4193
 
BseYI C'CCAG_C

Cuts at:    876    1648    2406    3071    3479     876 
   Size:        772     758     665     408    1620

  Fragments arranged by size: 

                1620     772     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104     741    1765    1811    3041     104 
   Size:        637    1024      46    1230    1286

  Fragments arranged by size: 

                1286    1230    1024     637      46
 
BsiHKAI G_wGCw'C

Cuts at:    270    1237    2015    2205    3065     270 
   Size:        967     778     190     860    1428

  Fragments arranged by size: 

                1428     967     860     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    270     592    1006    1237    1848    1941    2015    2205    2267 
   Size:        322     414     231     611      93      74     190      62

Cuts at:   2267    3065     270 
   Size:        798    1428

  Fragments arranged by size: 

                1428     798     611     414     322     231     190      93
                  74      62
 
BspHI T'CATG_A

Cuts at:   2655    2655 
   Size:       4223
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1273    1487    1789    2170    1273 
   Size:        214     302     381    3326

  Fragments arranged by size: 

                3326     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     638    2515    2569    4053    4218      50 
   Size:        588    1877      54    1484     165      55

  Fragments arranged by size: 

                1877    1484     588     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2135    2135 
   Size:       4223
 
BsrFI r'CCGG_y

Cuts at:   2221    2402    2221 
   Size:        181    4042
 
BssSI C'ACGA_G

Cuts at:   2494    3202    2494 
   Size:        708    3515
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1600    1600 
   Size:       4223
 
BstBI TT'CG_AA

Cuts at:   2584    2584 
   Size:       4223
 
BstEII G'GTnAC_C

Cuts at:   1190    1190 
   Size:       4223
 
BstXI CCAn_nnnn'nTGG

Cuts at:    876     876 
   Size:       4223
 
BstYI r'GATC_y

Cuts at:      1     288     313    1248    1715    1738    2073    2319    2625 
   Size:        287      25     935     467      23     335     246     306

Cuts at:   2625    2637    2723    3775       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1052     935     467     449     335     306     287     246
                  86      25      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    660     660 
   Size:       4223
 
BtgI C'CryG_G

Cuts at:    107     560     107 
   Size:        453    3770
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2173    2354    2517    2173 
   Size:        181     163    3879

  Fragments arranged by size: 

                3879     181     163
 
BtsI GCAGTG_nn'

Cuts at:    428    1466     428 
   Size:       1038    3185
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    687     722     854     889     687 
   Size:         35     132      35    4021

  Fragments arranged by size: 

                4021     132      35      35
 
DraI TTT'AAA

Cuts at:   1222    1222 
   Size:       4223
 
DrdI GACnn_nn'nnGTC

Cuts at:   1929    3273    1929 
   Size:       1344    2879
 
EaeI y'GGCC_r

Cuts at:    557     816    1808    1982    2373    2400    3745     557 
   Size:        259     992     174     391      27    1345    1035

  Fragments arranged by size: 

                1345    1035     992     391     259     174      27
 
EagI C'GGCC_G

Cuts at:   1808    1808 
   Size:       4223
 
EarI CTCTTCn'nnn_

Cuts at:    448    2246    2456     448 
   Size:       1798     210    2215

  Fragments arranged by size: 

                2215    1798     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2431    3173    3319    4033    2431 
   Size:        742     146     714    2621

  Fragments arranged by size: 

                2621     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1235    1235 
   Size:       4223
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    627     827    2048    2480    2833    3495     627 
   Size:        200    1221     432     353     662    1355

  Fragments arranged by size: 

                1355    1221     662     432     353     200
 
EcoO109I rG'GnC_Cy

Cuts at:    856    1002    1003    1045    1362     856 
   Size:        146       1      42     317    3717

  Fragments arranged by size: 

                3717     317     146      42       1
 
EcoRI G'AATT_C

Cuts at:   1227    1227 
   Size:       4223
 
EcoRV GAT'ATC

Cuts at:    306     306 
   Size:       4223
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1220    2242    1220 
   Size:       1022    3201
 
FspI TGC'GCA

Cuts at:   2004    2004 
   Size:       4223
 
HaeII r_GCGC'y

Cuts at:   1152    1657    1905    3135    3906    1152 
   Size:        505     248    1230     771    1469

  Fragments arranged by size: 

                1469    1230     771     505     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2065    2095    2097    2127    2065 
   Size:         30       2      30    4161

  Fragments arranged by size: 

                4161      30      30       2
 
HincII GTy'rAC

Cuts at:   1262    1262 
   Size:       4223
 
KasI G'GCGC_C

Cuts at:   1653    1901    1653 
   Size:        248    3975
 
KpnI G_GTAC'C

Cuts at:   1243    1243 
   Size:       4223
 
MfeI C'AATT_G

Cuts at:    831     831 
   Size:       4223
 
MluI A'CGCG_T

Cuts at:   3656    3656 
   Size:       4223
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    266     654    2982    3166     266 
   Size:        388    2328     184    1323

  Fragments arranged by size: 

                2328    1323     388     184
 
MscI TGG'CCA

Cuts at:    559     818    1984     559 
   Size:        259    1166    2798

  Fragments arranged by size: 

                2798    1166     259
 
MslI CAynn'nnrTG

Cuts at:   2212    2339    2212 
   Size:        127    4096
 
MspA1I CmG'CkG

Cuts at:    130     826    1075    1334    1648    2008    2792    3037     130 
   Size:        696     249     259     314     360     784     245    1316

  Fragments arranged by size: 

                1316     784     696     360     314     259     249     245
            
 
NaeI GCC'GGC

Cuts at:   2404    2404 
   Size:       4223
 
NarI GG'CG_CC

Cuts at:   1654    1902    1654 
   Size:        248    3975
 
NcoI C'CATG_G

Cuts at:    107     560     107 
   Size:        453    3770
 
NgoMIV G'CCGG_C

Cuts at:   2402    2402 
   Size:       4223
 
NheI G'CTAG_C

Cuts at:    837     837 
   Size:       4223
 
NspI r_CATG'y

Cuts at:   1276    1776    3379    1276 
   Size:        500    1603    2120

  Fragments arranged by size: 

                2120    1603     500
 
PciI A'CATG_T

Cuts at:   3375    3375 
   Size:       4223
 
PfoI T'CCnGG_A

Cuts at:    804     804 
   Size:       4223
 
PmeI GTTT'AAAC

Cuts at:   1222    1222 
   Size:       4223
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2669    2701    2669 
   Size:         32    4191
 
PpuMI rG'GwC_Cy

Cuts at:    856     856 
   Size:       4223
 
PspOMI G'GGCC_C

Cuts at:   1002    1002 
   Size:       4223
 
PstI C_TGCA'G

Cuts at:   1180    1270    1955    1180 
   Size:         90     685    3448

  Fragments arranged by size: 

                3448     685      90
 
PvuI CG_AT'CG

Cuts at:    104     741     104 
   Size:        637    3586
 
PvuII CAG'CTG

Cuts at:    130     826    1075    1648    2008     130 
   Size:        696     249     573     360    2345

  Fragments arranged by size: 

                2345     696     573     360     249
 
RsrII CG'GwC_CG

Cuts at:   2418    2418 
   Size:       4223
 
SacI G_AGCT'C

Cuts at:   1237    1237 
   Size:       4223
 
SalI G'TCGA_C

Cuts at:   1260    1260 
   Size:       4223
 
SanDI GG'GwC_CC

Cuts at:    856     856 
   Size:       4223
 
SapI GCTCTTCn'nnn_

Cuts at:    448    2246    2456     448 
   Size:       1798     210    2215

  Fragments arranged by size: 

                2215    1798     210
 
SbfI CC_TGCA'GG

Cuts at:   1270    1270 
   Size:       4223
 
ScaI AGT'ACT

Cuts at:   3740    3740 
   Size:       4223
 
SexAI A'CCwGG_T

Cuts at:   1010    1010 
   Size:       4223
 
SfcI C'TryA_G

Cuts at:     33     117    1049    1176    1266    1951    2919    3110      33 
   Size:         84     932     127      90     685     968     191    1146

  Fragments arranged by size: 

                1146     968     932     685     191     127      90      84
            
 
SfoI GGC'GCC

Cuts at:   1655    1903    1655 
   Size:        248    3975
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4223
 
SmaI CCC'GGG

Cuts at:   1245    1245 
   Size:       4223
 
SmlI C'TyrA_G

Cuts at:    715    1378    2293    3007    3269     715 
   Size:        663     915     714     262    1669

  Fragments arranged by size: 

                1669     915     714     663     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4223
 
SphI G_CATG'C

Cuts at:   1276    1776    1276 
   Size:        500    3723
 
StyI C'CwwG_G

Cuts at:    107     185     560    1195    1357    1711     107 
   Size:         78     375     635     162     354    2619

  Fragments arranged by size: 

                2619     635     375     354     162      78
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    882    1806    1939    2398    2607     882 
   Size:        924     133     459     209    2498

  Fragments arranged by size: 

                2498     924     459     209     133
 
TatI w'GTAC_w

Cuts at:   3738    3738 
   Size:       4223
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    194     226    3740    3772     194 
   Size:         32    3514      32     645

  Fragments arranged by size: 

                3514     645      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    549    2020     549 
   Size:       1471    2752
 
XbaI T'CTAG_A

Cuts at:   1254    1254 
   Size:       4223
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1718    1718 
   Size:       4223
 
XmaI C'CCGG_G

Cuts at:   1243    1243 
   Size:       4223
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NspI     PciI     PfoI     PmeI
     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI     BglI
    BseRI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BsrGI   BssHII
  BstZ17I     ClaI   DraIII    EcoNI     FseI    FspAI  HindIII     HpaI
     NdeI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmlI
    PshAI     PsiI    PspXI     PsrI    SacII     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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