Restriction map for pF1KB4699
 (Linear) (Six-base) MAPSORT of: KIBB4699.seq  Check: 7623  from: 1  to: 1110



KIBB4699.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AccI GT'mk_AC

Cuts at:      0     602    1110 
   Size:        602     508
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     519    1110 
   Size:        519     591
 
AflIII A'CryG_T

Cuts at:      0     409    1110 
   Size:        409     701
 
AlwNI CAG_nnn'CTG

Cuts at:      0     970    1110 
   Size:        970     140
 
ApaI G_GGCC'C

Cuts at:      0     898    1110 
   Size:        898     212
 
BanII G_rGCy'C

Cuts at:      0     898    1110 
   Size:        898     212
 
BbvCI CC'TCA_GC

Cuts at:      0    1036    1110 
   Size:       1036      74
 
BglII A'GATC_T

Cuts at:      0     205    1110 
   Size:        205     905
 
Bme1580I G_kGCm'C

Cuts at:      0     484     898    1110 
   Size:        484     414     212

  Fragments arranged by size: 

                 484     414     212
 
BmgBI CAC'GTC

Cuts at:      0     410     477    1110 
   Size:        410      67     633

  Fragments arranged by size: 

                 633     410      67
 
BmtI G_CTAG'C

Cuts at:      0     733    1110 
   Size:        733     377
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     719    1110 
   Size:        719     391
 
Bpu10I CC'TnA_GC

Cuts at:      0    1036    1110 
   Size:       1036      74
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     592    1110 
   Size:        592     518
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      86     116    1110 
   Size:         86      30     994

  Fragments arranged by size: 

                 994      86      30
 
BseYI C'CCAG_C

Cuts at:      0     768    1110 
   Size:        768     342
 
BsiEI CG_ry'CG

Cuts at:      0     633    1110 
   Size:        633     477
 
BsiHKAI G_wGCw'C

Cuts at:      0     162    1110 
   Size:        162     948
 
Bsp1286I G_dGCh'C

Cuts at:      0     162     484     898    1110 
   Size:        162     322     414     212

  Fragments arranged by size: 

                 414     322     212     162
 
BsrBI CCG'CTC

Cuts at:      0     530    1110 
   Size:        530     580
 
BstEII G'GTnAC_C

Cuts at:      0    1082    1110 
   Size:       1082      28
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     768    1110 
   Size:        768     342
 
BstYI r'GATC_y

Cuts at:      0     180     205    1110 
   Size:        180      25     905

  Fragments arranged by size: 

                 905     180      25
 
Bsu36I CC'TnA_GG

Cuts at:      0     552    1110 
   Size:        552     558
 
BtgI C'CryG_G

Cuts at:      0     452    1110 
   Size:        452     658
 
BtsI GCAGTG_nn'

Cuts at:      0     320    1110 
   Size:        320     790
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     579     614     746     781    1110 
   Size:        579      35     132      35     329

  Fragments arranged by size: 

                 579     329     132      35      35
 
EaeI y'GGCC_r

Cuts at:      0     449     708    1110 
   Size:        449     259     402

  Fragments arranged by size: 

                 449     402     259
 
EarI CTCTTCn'nnn_

Cuts at:      0     340    1110 
   Size:        340     770
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     519     719    1110 
   Size:        519     200     391

  Fragments arranged by size: 

                 519     391     200
 
EcoO109I rG'GnC_Cy

Cuts at:      0     748     894     895     937    1110 
   Size:        748     146       1      42     173

  Fragments arranged by size: 

                 748     173     146      42       1
 
EcoRV GAT'ATC

Cuts at:      0     198    1110 
   Size:        198     912
 
HaeII r_GCGC'y

Cuts at:      0    1044    1110 
   Size:       1044      66
 
MfeI C'AATT_G

Cuts at:      0     723    1110 
   Size:        723     387
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     546    1110 
   Size:        158     388     564

  Fragments arranged by size: 

                 564     388     158
 
MscI TGG'CCA

Cuts at:      0     451     710    1110 
   Size:        451     259     400

  Fragments arranged by size: 

                 451     400     259
 
MspA1I CmG'CkG

Cuts at:      0      22     718     967    1110 
   Size:         22     696     249     143

  Fragments arranged by size: 

                 696     249     143      22
 
NcoI C'CATG_G

Cuts at:      0     452    1110 
   Size:        452     658
 
NheI G'CTAG_C

Cuts at:      0     729    1110 
   Size:        729     381
 
PfoI T'CCnGG_A

Cuts at:      0     696    1110 
   Size:        696     414
 
PpuMI rG'GwC_Cy

Cuts at:      0     748    1110 
   Size:        748     362
 
PspOMI G'GGCC_C

Cuts at:      0     894    1110 
   Size:        894     216
 
PstI C_TGCA'G

Cuts at:      0    1072    1110 
   Size:       1072      38
 
PvuI CG_AT'CG

Cuts at:      0     633    1110 
   Size:        633     477
 
PvuII CAG'CTG

Cuts at:      0      22     718     967    1110 
   Size:         22     696     249     143

  Fragments arranged by size: 

                 696     249     143      22
 
SanDI GG'GwC_CC

Cuts at:      0     748    1110 
   Size:        748     362
 
SapI GCTCTTCn'nnn_

Cuts at:      0     340    1110 
   Size:        340     770
 
SexAI A'CCwGG_T

Cuts at:      0     902    1110 
   Size:        902     208
 
SfcI C'TryA_G

Cuts at:      0       9     941    1068    1110 
   Size:          9     932     127      42

  Fragments arranged by size: 

                 932     127      42       9
 
SmlI C'TyrA_G

Cuts at:      0     607    1110 
   Size:        607     503
 
StyI C'CwwG_G

Cuts at:      0      77     452    1087    1110 
   Size:         77     375     635      23

  Fragments arranged by size: 

                 635     375      77      23
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     774    1110 
   Size:        774     336
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      86     118    1110 
   Size:         86      32     992

  Fragments arranged by size: 

                 992      86      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     441    1110 
   Size:        441     669
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApaI    BanII    BbvCI    BglII
 Bme1580I    BmgBI     BmtI     BpmI   Bpu10I    BpuEI    BsaXI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BsrBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI    CspCI     EaeI     EarI  Eco57MI EcoO109I    EcoRV
    HaeII     MfeI     MmeI     MscI   MspA1I     NcoI     NheI     PfoI
    PpuMI   PspOMI     PstI     PvuI    PvuII    SanDI     SapI    SexAI
     SfcI     SmlI     StyI    TaqII     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI     BmrI     BplI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI  BstZ17I    BtgZI     ClaI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MslI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI    RsrII     SacI    SacII
     SalI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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