Restriction map for pF1KB4698
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       AccII       AccIII      AflII       AgeI        
Alw44I      AlwNI       ApaLI       AscI        AseI        
AsnI        Asp700I     AspI        AviII       BalI        
BbrPI       BbsI        BcgI        BciVI       BfrI        
BglI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaWI       BseAI       BsgI        BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       BstBI       
BstEII      BstPI       BstUI       CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EcoO65I     FauI        FseI        FspI        
HindIII     HpaI        KspI        MluI        MluNI       
MroI        MscI        MspA1I      MvnI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       PvuI        PvuII       RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        695  GGTACC               G^GTACC
AclI         2434  AACGTT               AA^CGTT
AflIII        684  ACACGT               A^C[GA][CT]GT
AhdI          277  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          939  CCTAAGG              CC^T[ACGT]AGG
Aor51HI      1319  AGCGCT               AGC^GCT
Asp718I       695  GGTACC               G^GTACC
AspEI         277  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1555  CTCGGG               C^[CT]CG[GA]G
AvaII        2075  GGACC                G^G[AT]CC
AvrII         100  CCTAGG               C^CTAGG
BamHI        2278  GGATCC               G^GATCC
BbeI          106  GGCGCC               GGCGC^C
BbvCI        1969  GCTGAGG              CCTCAGC(-5/-2)
BcnI         1148  CCCGG                CC^[GC]GG
BlnI          100  CCTAGG               C^CTAGG
BsiEI        2120  CGGCCG               CG[GA][CT]^CG
BsmBI         324  CGTCTC               CGTCTC(1/5)
BsoBI        1555  CTCGGG               C^[CT]CG[GA]G
Bst1107I      882  GTATAC               GTA^TAC
BstXI        1256  CCACAATTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       882  GTATAC               GTA^TAC
Bsu36I        939  CCTAAGG              CC^T[ACGT]AGG
Cac8I         483  GCAAGC               GC[ACGT]^[ACGT]GC
EaeI         2120  CGGCCG               [CT]^GGCC[GA]
EagI         2120  CGGCCG               C^GGCCG
Eam1105I      277  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2120  CGGCCG               C^GGCCG
Eco47III     1319  AGCGCT               AGC^GCT
Eco52I       2120  CGGCCG               C^GGCCG
Eco81I        939  CCTAAGG              CC^T[ACGT]AGG
EcoNI        1041  CCTCAAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2583  GAATTC               G^AATTC
EcoRV        1560  GATATC               GAT^ATC
EcoT22I        13  ATGCAT               ATGCA^T
HapII        1149  CCGG                 C^CGG
HgaI         2333  GCGTC                GACGC(5/10)
HpaII        1149  CCGG                 C^CGG
KasI          106  GGCGCC               G^GCGCC
KpnI          695  GGTACC               GGTAC^C
MslI          375  CATTCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1149  CCGG                 C^CGG
NarI          106  GGCGCC               GG^CGCC
NciI         1148  CCCGG                CC^[GC]GG
NsiI           13  ATGCAT               ATGCA^T
NspI         2081  ACATGC               [GA]CATG^[CT]
PflMI         349  CCAACAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     2434  AACGTT               AA^CGTT
PstI         1349  CTGCAG               CTGCA^G
SexAI         662  ACCAGGT              A^CC[AT]GGT
SfoI          106  GGCGCC               GGC^GCC
SmlI         1231  CTTGAG               C^T[CT][GA]AG
SspI         2560  AATATT               AAT^ATT
Van91I        349  CCAACAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1000  CCAGCAGTGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccI          658  GTAGAC               GT^[AC][GT]AC
              882  GTATAC              
AcyI          106  GGCGCC               G[GA]^CG[CT]C
             2332  GGCGTC              
ApaI         1037  GGGCCC               GGGCC^C
             1144  GGGCCC              
BclI         1571  TGATCA               T^GATCA
             1892  TGATCA              
BfaI          101  CTAG                 C^TAG
             2135  CTAG                
BsaBI        1560  GATATCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1893  GATCATAATC          
BsaHI         106  GGCGCC               G[GA]^CG[CT]C
             2332  GGCGTC              
BsmI          975  GAATGC               GAATGC(1/-1)
             2550  GAATGC              
BstAPI        483  GCAAGCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2545  GCAAAGAATGC         
FbaI         1571  TGATCA               T^GATCA
             1892  TGATCA              
HaeII         106  GGCGCC               [GA]GCGC^[CT]
             1319  AGCGCT              
Hin1I         106  GGCGCC               G[GA]^CG[CT]C
             2332  GGCGTC              
MaeI          101  CTAG                 C^TAG
             2135  CTAG                
MamI         1560  GATATCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1893  GATCATAATC          
MfeI         1391  CAATTG               C^AATTG
             1487  CAATTG              
MunI         1391  CAATTG               C^AATTG
             1487  CAATTG              
SfcI         1349  CTGCAG               C^T[GA][CT]AG
             1937  CTGTAG              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       BanI        BanII       BbvI        
BglII       BmyI        BpmI        BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspHI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       CfoI        
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HhaI        HinP1I      HincII      HindII      
HinfI       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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