Restriction map for pF1KB4698
 (Linear) (Six-base) MAPSORT of: KIBB4698.seq  Check: 5333  from: 1  to: 2649



KIBB4698.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AccI GT'mk_AC

Cuts at:      0     659     883    2649 
   Size:        659     224    1766

  Fragments arranged by size: 

                1766     659     224
 
Acc65I G'GTAC_C

Cuts at:      0     695    2649 
   Size:        695    1954
 
AclI AA'CG_TT

Cuts at:      0    2435    2649 
   Size:       2435     214
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     944    2649 
   Size:        944    1705
 
AfeI AGC'GCT

Cuts at:      0    1321    2649 
   Size:       1321    1328
 
AflIII A'CryG_T

Cuts at:      0     684    2649 
   Size:        684    1965
 
AhdI GACnn_n'nnGTC

Cuts at:      0     282    2649 
   Size:        282    2367
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     542    2649 
   Size:        542    2107
 
ApaI G_GGCC'C

Cuts at:      0    1041    1148    2649 
   Size:       1041     107    1501

  Fragments arranged by size: 

                1501    1041     107
 
ApoI r'AATT_y

Cuts at:      0     814    1436    1771    2583    2592    2649 
   Size:        814     622     335     812       9      57

  Fragments arranged by size: 

                 814     812     622     335      57       9
 
AvaI C'yCGr_G

Cuts at:      0    1555    2649 
   Size:       1555    1094
 
AvrII C'CTAG_G

Cuts at:      0     100    2649 
   Size:        100    2549
 
BamHI G'GATC_C

Cuts at:      0    2278    2649 
   Size:       2278     371
 
BanI G'GyrC_C

Cuts at:      0     106     695    1807    2649 
   Size:        106     589    1112     842

  Fragments arranged by size: 

                1112     842     589     106
 
BanII G_rGCy'C

Cuts at:      0     405     864    1041    1133    1148    1205    2099    2649 
   Size:        405     459     177      92      15      57     894     550

  Fragments arranged by size: 

                 894     550     459     405     177      92      57      15
            
 
BbeI G_GCGC'C

Cuts at:      0     110    2649 
   Size:        110    2539
 
BbvCI CC'TCA_GC

Cuts at:      0    1970    2649 
   Size:       1970     679
 
BclI T'GATC_A

Cuts at:      0    1571    1892    2649 
   Size:       1571     321     757

  Fragments arranged by size: 

                1571     757     321
 
BfrBI ATG'CAT

Cuts at:      0      15    2649 
   Size:         15    2634
 
BglII A'GATC_T

Cuts at:      0     591    1634    2044    2649 
   Size:        591    1043     410     605

  Fragments arranged by size: 

                1043     605     591     410
 
Bme1580I G_kGCm'C

Cuts at:      0    1010    1041    1148    2421    2649 
   Size:       1010      31     107    1273     228

  Fragments arranged by size: 

                1273    1010     228     107      31
 
BmgBI CAC'GTC

Cuts at:      0     324    2649 
   Size:        324    2325
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     231    2290    2649 
   Size:        231    2059     359

  Fragments arranged by size: 

                2059     359     231
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616     878    1185    2462    2649 
   Size:        616     262     307    1277     187

  Fragments arranged by size: 

                1277     616     307     262     187
 
Bpu10I CC'TnA_GC

Cuts at:      0     409    1970    2649 
   Size:        409    1561     679

  Fragments arranged by size: 

                1561     679     409
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1252    2649 
   Size:       1252    1397
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     378     728    1069    2649 
   Size:        378     350     341    1580

  Fragments arranged by size: 

                1580     378     350     341
 
BsaBI GATnn'nnATC

Cuts at:      0    1564    1897    2649 
   Size:       1564     333     752

  Fragments arranged by size: 

                1564     752     333
 
BsaHI Gr'CG_yC

Cuts at:      0     107    2333    2649 
   Size:        107    2226     316

  Fragments arranged by size: 

                2226     316     107
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1448    1478    2005    2035    2649 
   Size:       1448      30     527      30     614

  Fragments arranged by size: 

                1448     614     527      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     263    1738    1893    2649 
   Size:        263    1475     155     756

  Fragments arranged by size: 

                1475     756     263     155
 
BseYI C'CCAG_C

Cuts at:      0     340     999    2649 
   Size:        340     659    1650

  Fragments arranged by size: 

                1650     659     340
 
BsiEI CG_ry'CG

Cuts at:      0    2123    2649 
   Size:       2123     526
 
BsiHKAI G_wGCw'C

Cuts at:      0     405     864    1133    1205    2649 
   Size:        405     459     269      72    1444

  Fragments arranged by size: 

                1444     459     405     269      72
 
BsmI GAATG_Cn'

Cuts at:      0     981    2556    2649 
   Size:        981    1575      93

  Fragments arranged by size: 

                1575     981      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     330    2649 
   Size:        330    2319
 
Bsp1286I G_dGCh'C

Cuts at:      0     405     864    1010    1041    1133    1148    1205    2099 
   Size:        405     459     146      31      92      15      57     894

Cuts at:   2099    2421    2649 
   Size:        322     228

  Fragments arranged by size: 

                 894     459     405     322     228     146      92      57
                  31      15
 
BspHI T'CATG_A

Cuts at:      0    1296    1547    1568    2649 
   Size:       1296     251      21    1081

  Fragments arranged by size: 

                1296    1081     251      21
 
BsrDI GCAATG_nn'

Cuts at:      0     707    1320    2486    2649 
   Size:        707     613    1166     163

  Fragments arranged by size: 

                1166     707     613     163
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     489    2551    2649 
   Size:        489    2062      98

  Fragments arranged by size: 

                2062     489      98
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1263    2649 
   Size:       1263    1386
 
BstYI r'GATC_y

Cuts at:      0     591    1634    1843    1974    2044    2278    2649 
   Size:        591    1043     209     131      70     234     371

  Fragments arranged by size: 

                1043     591     371     234     209     131      70
 
BstZ17I GTA'TAC

Cuts at:      0     884    2649 
   Size:        884    1765
 
Bsu36I CC'TnA_GG

Cuts at:      0     940    2649 
   Size:        940    1709
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2247    2649 
   Size:       2247     402
 
BtsI GCAGTG_nn'

Cuts at:      0     478    1010    2063    2649 
   Size:        478     532    1053     586

  Fragments arranged by size: 

                1053     586     532     478
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1350    1385    2468    2503    2649 
   Size:       1350      35    1083      35     146

  Fragments arranged by size: 

                1350    1083     146      35      35
 
EaeI y'GGCC_r

Cuts at:      0    2120    2649 
   Size:       2120     529
 
EagI C'GGCC_G

Cuts at:      0    2120    2649 
   Size:       2120     529
 
EarI CTCTTCn'nnn_

Cuts at:      0      72    1514    1734    2649 
   Size:         72    1442     220     915

  Fragments arranged by size: 

                1442     915     220      72
 
EcoICRI GAG'CTC

Cuts at:      0     403     862    1131    1203    2649 
   Size:        403     459     269      72    1446

  Fragments arranged by size: 

                1446     459     403     269      72
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616     878     944    1185    2462    2649 
   Size:        616     262      66     241    1277     187

  Fragments arranged by size: 

                1277     616     262     241     187      66
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1045    2649 
   Size:       1045    1604
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1037    1038    1145    2466    2649 
   Size:       1037       1     107    1321     183

  Fragments arranged by size: 

                1321    1037     183     107       1
 
EcoRI G'AATT_C

Cuts at:      0    2583    2649 
   Size:       2583      66
 
EcoRV GAT'ATC

Cuts at:      0    1562    2649 
   Size:       1562    1087
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2228    2649 
   Size:       2228     421
 
HaeII r_GCGC'y

Cuts at:      0     110    1323    2649 
   Size:        110    1213    1326

  Fragments arranged by size: 

                1326    1213     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     268     300     377     409    1938    1970    2005    2037 
   Size:        268      32      77      32    1529      32      35      32

Cuts at:   2037    2649 
   Size:        612

  Fragments arranged by size: 

                1529     612     268      77      35      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     833    1341    1388    2649 
   Size:        833     508      47    1261

  Fragments arranged by size: 

                1261     833     508      47
 
KasI G'GCGC_C

Cuts at:      0     106    2649 
   Size:        106    2543
 
KpnI G_GTAC'C

Cuts at:      0     699    2649 
   Size:        699    1950
 
MfeI C'AATT_G

Cuts at:      0    1391    1487    2649 
   Size:       1391      96    1162

  Fragments arranged by size: 

                1391    1162      96
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1434    1845    2153    2649 
   Size:       1434     411     308     496

  Fragments arranged by size: 

                1434     496     411     308
 
MslI CAynn'nnrTG

Cuts at:      0     379    2649 
   Size:        379    2270
 
NarI GG'CG_CC

Cuts at:      0     107    2649 
   Size:        107    2542
 
NsiI A_TGCA'T

Cuts at:      0      17    2649 
   Size:         17    2632
 
NspI r_CATG'y

Cuts at:      0    2085    2649 
   Size:       2085     564
 
PasI CC'CwG_GG

Cuts at:      0    1010    2649 
   Size:       1010    1639
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     355    2649 
   Size:        355    2294
 
PsiI TTA'TAA

Cuts at:      0    2606    2649 
   Size:       2606      43
 
PspOMI G'GGCC_C

Cuts at:      0    1037    1144    2649 
   Size:       1037     107    1505

  Fragments arranged by size: 

                1505    1037     107
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     128     160    2649 
   Size:        128      32    2489

  Fragments arranged by size: 

                2489     128      32
 
PstI C_TGCA'G

Cuts at:      0    1353    2649 
   Size:       1353    1296
 
SacI G_AGCT'C

Cuts at:      0     405     864    1133    1205    2649 
   Size:        405     459     269      72    1444

  Fragments arranged by size: 

                1444     459     405     269      72
 
SexAI A'CCwGG_T

Cuts at:      0     662    2649 
   Size:        662    1987
 
SfcI C'TryA_G

Cuts at:      0    1349    1937    2649 
   Size:       1349     588     712

  Fragments arranged by size: 

                1349     712     588
 
SfoI GGC'GCC

Cuts at:      0     108    2649 
   Size:        108    2541
 
SmlI C'TyrA_G

Cuts at:      0    1231    2649 
   Size:       1231    1418
 
SspI AAT'ATT

Cuts at:      0    2562    2649 
   Size:       2562      87
 
StyI C'CwwG_G

Cuts at:      0     100    1802    2387    2469    2649 
   Size:        100    1702     585      82     180

  Fragments arranged by size: 

                1702     585     180     100      82
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1795    2649 
   Size:       1795     854
 
TatI w'GTAC_w

Cuts at:      0    1379    2192    2649 
   Size:       1379     813     457

  Fragments arranged by size: 

                1379     813     457
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1007    2649 
   Size:       1007    1642
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AhdI     AlfI
     ApaI     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
    BbvCI     BclI    BfrBI    BglII Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BsrDI   BstAPI
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI    CspCI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI     MmeI
     MslI     NarI     NsiI     NspI     PasI    PflMI     PsiI   PspOMI
     PsrI     PstI     SacI    SexAI     SfcI     SfoI     SmlI     SspI
     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AgeI     AleI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI     BaeI     BbsI     BcgI    BciVI     BglI
     BlpI     BmtI     BplI    BsaAI    BsaWI     BsgI    BsiWI    BspEI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
     BtgI     ClaI     DraI   DraIII     DrdI     EciI     FseI     FspI
    FspAI  HindIII     HpaI     MluI     MscI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI    PspXI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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