Restriction map for
pF1KB4689
(Circular) (Six-base) MAPSORT of: pF1KB4689.seq Check: 8546 from: 1 to: 4433
pF1KB4689 4433 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1471 1471
Size: 4433
Acc65I G'GTAC_C
Cuts at: 1449 1449
Size: 4433
AclI AA'CG_TT
Cuts at: 4273 4273
Size: 4433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 430 2258 2690 3043 3705 238
Size: 192 1828 432 353 662 966
Fragments arranged by size:
1828 966 662 432 353 192
AflIII A'CryG_T
Cuts at: 1785 3585 3866 1785
Size: 1800 281 2352
Fragments arranged by size:
2352 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1738 3176 1738
Size: 1438 2995
ApaLI G'TGCA_C
Cuts at: 698 3271 698
Size: 2573 1860
ApoI r'AATT_y
Cuts at: 160 404 1437 3786 4042 160
Size: 244 1033 2349 256 551
Fragments arranged by size:
2349 1033 551 256 244
AseI AT'TA_AT
Cuts at: 20 20
Size: 4433
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
AvaI C'yCGr_G
Cuts at: 358 1453 358
Size: 1095 3338
BamHI G'GATC_C
Cuts at: 1321 1458 1321
Size: 137 4296
BanI G'GyrC_C
Cuts at: 1449 1863 2111 2146 1449
Size: 414 248 35 3736
Fragments arranged by size:
3736 414 248 35
BanII G_rGCy'C
Cuts at: 889 948 1096 1447 2477 889
Size: 59 148 351 1030 2845
Fragments arranged by size:
2845 1030 351 148 59
BbeI G_GCGC'C
Cuts at: 1867 2115 1867
Size: 248 4185
BciVI GTATCCnnnnn_n'
Cuts at: 2331 3387 4433 2331
Size: 1056 1046 2331
Fragments arranged by size:
2331 1056 1046
BclI T'GATC_A
Cuts at: 1953 1953
Size: 4433
BglII A'GATC_T
Cuts at: 211 1948 3985 211
Size: 1737 2037 659
Fragments arranged by size:
2037 1737 659
BlpI GC'TnA_GC
Cuts at: 1545 1545
Size: 4433
Bme1580I G_kGCm'C
Cuts at: 702 2058 2151 3275 702
Size: 1356 93 1124 1860
Fragments arranged by size:
1860 1356 1124 93
BmgBI CAC'GTC
Cuts at: 697 697
Size: 4433
BmrI ACTGGGnnnn_n'
Cuts at: 778 1645 1726 2061 4205 778
Size: 867 81 335 2144 1006
Fragments arranged by size:
2144 1006 867 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 4433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1043 1043
Size: 4433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1609 2488 3202 3500 1609
Size: 879 714 298 2542
Fragments arranged by size:
2542 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 983 1011 1019 4137 983
Size: 28 8 3118 1279
Fragments arranged by size:
3118 1279 28 8
BsaAI yAC'GTr
Cuts at: 1786 1786
Size: 4433
BsaBI GATnn'nnATC
Cuts at: 6 1947 3984 6
Size: 1941 2037 455
Fragments arranged by size:
2037 1941 455
BsaHI Gr'CG_yC
Cuts at: 780 846 1864 2112 2814 4310 780
Size: 66 1018 248 702 1496 903
Fragments arranged by size:
1496 1018 903 702 248 66
BsaWI w'CCGG_w
Cuts at: 1135 1622 2143 2821 3232 3379 3653 1135
Size: 487 521 678 411 147 274 1915
Fragments arranged by size:
1915 678 521 487 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 902 902
Size: 4433
BseYI C'CCAG_C
Cuts at: 268 1858 2616 3281 3689 268
Size: 1590 758 665 408 1012
Fragments arranged by size:
1590 1012 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 930 1975 2021 3251 104
Size: 826 1045 46 1230 1286
Fragments arranged by size:
1286 1230 1045 826 46
BsiHKAI G_wGCw'C
Cuts at: 702 948 1447 2225 2415 3275 702
Size: 246 499 778 190 860 1860
Fragments arranged by size:
1860 860 778 499 246 190
BsmI GAATG_Cn'
Cuts at: 245 270 589 830 1377 245
Size: 25 319 241 547 3301
Fragments arranged by size:
3301 547 319 241 25
Bsp1286I G_dGCh'C
Cuts at: 702 889 948 1096 1447 2058 2151 2225 2415
Size: 187 59 148 351 611 93 74 190
Cuts at: 2415 2477 3275 702
Size: 62 798 1860
Fragments arranged by size:
1860 798 611 351 190 187 148 93
74 62 59
BspEI T'CCGG_A
Cuts at: 1135 1135
Size: 4433
BspHI T'CATG_A
Cuts at: 1278 2865 1278
Size: 1587 2846
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1483 1697 1999 2380 1483
Size: 214 302 381 3536
Fragments arranged by size:
3536 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2725 2779 4263 4428 50
Size: 2675 54 1484 165 55
Fragments arranged by size:
2675 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2345 2345
Size: 4433
BsrFI r'CCGG_y
Cuts at: 2431 2612 2431
Size: 181 4252
BssSI C'ACGA_G
Cuts at: 2704 3412 2704
Size: 708 3725
BstAPI GCAn_nnn'nTGC
Cuts at: 1810 1810
Size: 4433
BstBI TT'CG_AA
Cuts at: 2794 2794
Size: 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 1103 1227 1103
Size: 124 4309
BstYI r'GATC_y
Cuts at: 1 211 1234 1321 1458 1925 1948 2283 2529
Size: 210 1023 87 137 467 23 335 246
Cuts at: 2529 2835 2847 2933 3985 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 1023 467 449 335 306 246 210
137 87 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4433
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2383 2564 2727 2383
Size: 181 163 4089
Fragments arranged by size:
4089 181 163
BtsI GCAGTG_nn'
Cuts at: 281 357 1676 281
Size: 76 1319 3038
Fragments arranged by size:
3038 1319 76
DraI TTT'AAA
Cuts at: 207 1432 207
Size: 1225 3208
DraIII CAC_nnn'GTG
Cuts at: 1129 1129
Size: 4433
DrdI GACnn_nn'nnGTC
Cuts at: 2139 3483 2139
Size: 1344 3089
EaeI y'GGCC_r
Cuts at: 927 2018 2192 2583 2610 3955 927
Size: 1091 174 391 27 1345 1405
Fragments arranged by size:
1405 1345 1091 391 174 27
EagI C'GGCC_G
Cuts at: 927 2018 927
Size: 1091 3342
EarI CTCTTCn'nnn_
Cuts at: 185 428 801 861 965 1005 2456 2666 185
Size: 243 373 60 104 40 1451 210 1952
Fragments arranged by size:
1952 1451 373 243 210 104 60 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2641 3383 3529 4243 2641
Size: 742 146 714 2831
Fragments arranged by size:
2831 742 714 146
EcoICRI GAG'CTC
Cuts at: 946 1445 946
Size: 499 3934
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 430 1043 2258 2690 3043 3705 238
Size: 192 613 1215 432 353 662 966
Fragments arranged by size:
1215 966 662 613 432 353 192
EcoNI CCTnn'n_nnAGG
Cuts at: 893 1316 893
Size: 423 4010
EcoO109I rG'GnC_Cy
Cuts at: 1572 1572
Size: 4433
EcoRI G'AATT_C
Cuts at: 1437 1437
Size: 4433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4433
FspI TGC'GCA
Cuts at: 2214 2214
Size: 4433
HaeII r_GCGC'y
Cuts at: 1867 2115 3345 4116 1867
Size: 248 1230 771 2184
Fragments arranged by size:
2184 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 658 690 876 908 1280 1312 2275 2305 2307
Size: 32 186 32 372 32 963 30 2
Cuts at: 2307 2337 658
Size: 30 2754
Fragments arranged by size:
2754 963 372 186 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1472 1472
Size: 4433
HindIII A'AGCT_T
Cuts at: 571 571
Size: 4433
KasI G'GCGC_C
Cuts at: 1863 2111 1863
Size: 248 4185
KpnI G_GTAC'C
Cuts at: 1453 1453
Size: 4433
MfeI C'AATT_G
Cuts at: 395 395
Size: 4433
MluI A'CGCG_T
Cuts at: 3866 3866
Size: 4433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3192 3376 3192
Size: 184 4249
MscI TGG'CCA
Cuts at: 2194 2194
Size: 4433
MslI CAynn'nnrTG
Cuts at: 1101 2422 2549 1101
Size: 1321 127 2985
Fragments arranged by size:
2985 1321 127
MspA1I CmG'CkG
Cuts at: 1260 1544 1858 2218 3002 3247 1260
Size: 284 314 360 784 245 2446
Fragments arranged by size:
2446 784 360 314 284 245
NaeI GCC'GGC
Cuts at: 2614 2614
Size: 4433
NarI GG'CG_CC
Cuts at: 1864 2112 1864
Size: 248 4185
NcoI C'CATG_G
Cuts at: 107 107
Size: 4433
NdeI CA'TA_TG
Cuts at: 1173 1173
Size: 4433
NgoMIV G'CCGG_C
Cuts at: 2612 2612
Size: 4433
NspI r_CATG'y
Cuts at: 1486 1986 3589 1486
Size: 500 1603 2330
Fragments arranged by size:
2330 1603 500
PciI A'CATG_T
Cuts at: 3585 3585
Size: 4433
PflMI CCAn_nnn'nTGG
Cuts at: 1129 1129
Size: 4433
PfoI T'CCnGG_A
Cuts at: 578 915 578
Size: 337 4096
PmeI GTTT'AAAC
Cuts at: 1432 1432
Size: 4433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2879 2911 2879
Size: 32 4401
PstI C_TGCA'G
Cuts at: 180 1391 1480 2165 180
Size: 1211 89 685 2448
Fragments arranged by size:
2448 1211 685 89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4433
PvuII CAG'CTG
Cuts at: 1858 2218 1858
Size: 360 4073
RsrII CG'GwC_CG
Cuts at: 2628 2628
Size: 4433
SacI G_AGCT'C
Cuts at: 948 1447 948
Size: 499 3934
SalI G'TCGA_C
Cuts at: 1470 1470
Size: 4433
SapI GCTCTTCn'nnn_
Cuts at: 2456 2666 2456
Size: 210 4223
SbfI CC_TGCA'GG
Cuts at: 1480 1480
Size: 4433
ScaI AGT'ACT
Cuts at: 3950 3950
Size: 4433
SfcI C'TryA_G
Cuts at: 33 176 1387 1476 2161 3129 3320 33
Size: 143 1211 89 685 968 191 1146
Fragments arranged by size:
1211 1146 968 685 191 143 89
SfoI GGC'GCC
Cuts at: 1865 2113 1865
Size: 248 4185
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
SmaI CCC'GGG
Cuts at: 1455 1455
Size: 4433
SmlI C'TyrA_G
Cuts at: 1588 2503 3217 3479 1588
Size: 915 714 262 2542
Fragments arranged by size:
2542 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4433
SphI G_CATG'C
Cuts at: 1486 1986 1486
Size: 500 3933
SspI AAT'ATT
Cuts at: 300 300
Size: 4433
StyI C'CwwG_G
Cuts at: 107 428 1567 1921 107
Size: 321 1139 354 2619
Fragments arranged by size:
2619 1139 354 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2016 2149 2608 2817 2016
Size: 133 459 209 3632
Fragments arranged by size:
3632 459 209 133
TatI w'GTAC_w
Cuts at: 3948 3948
Size: 4433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 770 802 3950 3982 770
Size: 32 3148 32 1221
Fragments arranged by size:
3148 1221 32 32
Tth111I GACn'n_nGTC
Cuts at: 2230 2230
Size: 4433
XbaI T'CTAG_A
Cuts at: 214 862 1464 214
Size: 648 602 3183
Fragments arranged by size:
3183 648 602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1928 1928
Size: 4433
XmaI C'CCGG_G
Cuts at: 1453 1453
Size: 4433
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BglI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HpaI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI