Restriction map for pF1KB4689
 (Linear) (Six-base) MAPSORT of: KIBB4689.seq  Check: 1698  from: 1  to: 1320



KIBB4689.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     322    1320 
   Size:        130     192     998

  Fragments arranged by size: 

                 998     192     130
 
ApaLI G'TGCA_C

Cuts at:      0     590    1320 
   Size:        590     730
 
ApoI r'AATT_y

Cuts at:      0      52     296    1320 
   Size:         52     244    1024

  Fragments arranged by size: 

                1024     244      52
 
AvaI C'yCGr_G

Cuts at:      0     250    1320 
   Size:        250    1070
 
BamHI G'GATC_C

Cuts at:      0    1213    1320 
   Size:       1213     107
 
BanII G_rGCy'C

Cuts at:      0     781     840     988    1320 
   Size:        781      59     148     332

  Fragments arranged by size: 

                 781     332     148      59
 
BglII A'GATC_T

Cuts at:      0     103    1320 
   Size:        103    1217
 
Bme1580I G_kGCm'C

Cuts at:      0     594    1320 
   Size:        594     726
 
BmgBI CAC'GTC

Cuts at:      0     589    1320 
   Size:        589     731
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     670    1320 
   Size:        670     650
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     935    1320 
   Size:        935     385
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     875     903     911    1320 
   Size:        875      28       8     409

  Fragments arranged by size: 

                 875     409      28       8
 
BsaHI Gr'CG_yC

Cuts at:      0     672     738    1320 
   Size:        672      66     582

  Fragments arranged by size: 

                 672     582      66
 
BsaWI w'CCGG_w

Cuts at:      0    1027    1320 
   Size:       1027     293
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     794    1320 
   Size:        794     526
 
BseYI C'CCAG_C

Cuts at:      0     160    1320 
   Size:        160    1160
 
BsiEI CG_ry'CG

Cuts at:      0     822    1320 
   Size:        822     498
 
BsiHKAI G_wGCw'C

Cuts at:      0     594     840    1320 
   Size:        594     246     480

  Fragments arranged by size: 

                 594     480     246
 
BsmI GAATG_Cn'

Cuts at:      0     137     162     481     722    1269    1320 
   Size:        137      25     319     241     547      51

  Fragments arranged by size: 

                 547     319     241     137      51      25
 
Bsp1286I G_dGCh'C

Cuts at:      0     594     781     840     988    1320 
   Size:        594     187      59     148     332

  Fragments arranged by size: 

                 594     332     187     148      59
 
BspEI T'CCGG_A

Cuts at:      0    1027    1320 
   Size:       1027     293
 
BspHI T'CATG_A

Cuts at:      0    1170    1320 
   Size:       1170     150
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     995    1119    1320 
   Size:        995     124     201

  Fragments arranged by size: 

                 995     201     124
 
BstYI r'GATC_y

Cuts at:      0     103    1126    1213    1320 
   Size:        103    1023      87     107

  Fragments arranged by size: 

                1023     107     103      87
 
BtsI GCAGTG_nn'

Cuts at:      0     173     249    1320 
   Size:        173      76    1071

  Fragments arranged by size: 

                1071     173      76
 
DraI TTT'AAA

Cuts at:      0      99    1320 
   Size:         99    1221
 
DraIII CAC_nnn'GTG

Cuts at:      0    1021    1320 
   Size:       1021     299
 
EaeI y'GGCC_r

Cuts at:      0     819    1320 
   Size:        819     501
 
EagI C'GGCC_G

Cuts at:      0     819    1320 
   Size:        819     501
 
EarI CTCTTCn'nnn_

Cuts at:      0      77     320     693     753     857     897    1320 
   Size:         77     243     373      60     104      40     423

  Fragments arranged by size: 

                 423     373     243     104      77      60      40
 
EcoICRI GAG'CTC

Cuts at:      0     838    1320 
   Size:        838     482
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     322     935    1320 
   Size:        130     192     613     385

  Fragments arranged by size: 

                 613     385     192     130
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     785    1208    1320 
   Size:        785     423     112

  Fragments arranged by size: 

                 785     423     112
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     550     582     768     800    1172    1204    1320 
   Size:        550      32     186      32     372      32     116

  Fragments arranged by size: 

                 550     372     186     116      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     463    1320 
   Size:        463     857
 
MfeI C'AATT_G

Cuts at:      0     287    1320 
   Size:        287    1033
 
MslI CAynn'nnrTG

Cuts at:      0     993    1320 
   Size:        993     327
 
MspA1I CmG'CkG

Cuts at:      0    1152    1320 
   Size:       1152     168
 
NdeI CA'TA_TG

Cuts at:      0    1065    1320 
   Size:       1065     255
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1021    1320 
   Size:       1021     299
 
PfoI T'CCnGG_A

Cuts at:      0     470     807    1320 
   Size:        470     337     513

  Fragments arranged by size: 

                 513     470     337
 
PstI C_TGCA'G

Cuts at:      0      72    1283    1320 
   Size:         72    1211      37

  Fragments arranged by size: 

                1211      72      37
 
SacI G_AGCT'C

Cuts at:      0     840    1320 
   Size:        840     480
 
SfcI C'TryA_G

Cuts at:      0      68    1279    1320 
   Size:         68    1211      41

  Fragments arranged by size: 

                1211      68      41
 
SspI AAT'ATT

Cuts at:      0     192    1320 
   Size:        192    1128
 
StyI C'CwwG_G

Cuts at:      0     320    1320 
   Size:        320    1000
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     662     694    1320 
   Size:        662      32     626

  Fragments arranged by size: 

                 662     626      32
 
XbaI T'CTAG_A

Cuts at:      0     106     754    1320 
   Size:        106     648     566

  Fragments arranged by size: 

                 648     566     106
 

 Enzymes that do cut:

     AcuI    ApaLI     ApoI     AvaI    BamHI    BanII    BglII Bme1580I
    BmgBI     BmrI     BpmI     BsaI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BstXI    BstYI
     BtsI     DraI   DraIII     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI    Hin4I  HindIII     MfeI     MslI   MspA1I     NdeI    PflMI
     PfoI     PstI     SacI     SfcI     SspI     StyI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaXI     BsgI    BsiWI    BsmBI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DrdI     EciI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TatI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI
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