Restriction map for pF1KB4679
 (Circular) (Six-base) MAPSORT of: pF1KB4679.seq  Check: 3474  from: 1  to: 3716



pF1KB4679  3716 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    529     529 
   Size:       3716
 
AccI GT'mk_AC

Cuts at:    754     754 
   Size:       3716
 
Acc65I G'GTAC_C

Cuts at:    350     732     350 
   Size:        382    3334
 
AclI AA'CG_TT

Cuts at:   3556    3556 
   Size:       3716
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1541    1973    2326    2988    1541 
   Size:        432     353     662    2269

  Fragments arranged by size: 

                2269     662     432     353
 
AflIII A'CryG_T

Cuts at:    209     670    1068    2868    3149     209 
   Size:        461     398    1800     281     776

  Fragments arranged by size: 

                1800     776     461     398     281
 
AleI CACnn'nnGTG

Cuts at:    530     530 
   Size:       3716
 
AlwNI CAG_nnn'CTG

Cuts at:   1021    2459    1021 
   Size:       1438    2278
 
ApaLI G'TGCA_C

Cuts at:   2554    2554 
   Size:       3716
 
ApoI r'AATT_y

Cuts at:    720    3069    3325     720 
   Size:       2349     256    1111

  Fragments arranged by size: 

                2349    1111     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3716
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3716
 
AvaI C'yCGr_G

Cuts at:    431     736     431 
   Size:        305    3411
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    691     724     691 
   Size:         33    3683
 
BamHI G'GATC_C

Cuts at:    741     741 
   Size:       3716
 
BanI G'GyrC_C

Cuts at:    350     732    1146    1394    1429     350 
   Size:        382     414     248      35    2637

  Fragments arranged by size: 

                2637     414     382     248      35
 
BanII G_rGCy'C

Cuts at:    429     730    1760     429 
   Size:        301    1030    2385

  Fragments arranged by size: 

                2385    1030     301
 
BbeI G_GCGC'C

Cuts at:   1150    1398    1150 
   Size:        248    3468
 
BbsI GAAGACnn'nnnn_

Cuts at:    317     317 
   Size:       3716
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    564     598     564 
   Size:         34    3682
 
BciVI GTATCCnnnnn_n'

Cuts at:    692    1614    2670    3716     692 
   Size:        922    1056    1046     692

  Fragments arranged by size: 

                1056    1046     922     692
 
BclI T'GATC_A

Cuts at:   1236    1236 
   Size:       3716
 
BglI GCCn_nnn'nGGC

Cuts at:    401     401 
   Size:       3716
 
BglII A'GATC_T

Cuts at:    649    1231    3268     649 
   Size:        582    2037    1097

  Fragments arranged by size: 

                2037    1097     582
 
BlpI GC'TnA_GC

Cuts at:    828     828 
   Size:       3716
 
Bme1580I G_kGCm'C

Cuts at:   1341    1434    2558    1341 
   Size:         93    1124    2499

  Fragments arranged by size: 

                2499    1124      93
 
BmgBI CAC'GTC

Cuts at:    210     210 
   Size:       3716
 
BmrI ACTGGGnnnn_n'

Cuts at:    928    1009    1344    3488     928 
   Size:         81     335    2144    1156

  Fragments arranged by size: 

                2144    1156     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2083    2083 
   Size:       3716
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    164     164 
   Size:       3716
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    232     892    1771    2485    2783     232 
   Size:        660     879     714     298    1165

  Fragments arranged by size: 

                1165     879     714     660     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3420    3420 
   Size:       3716
 
BsaAI yAC'GTr

Cuts at:    165     673    1069     165 
   Size:        508     396    2812

  Fragments arranged by size: 

                2812     508     396
 
BsaBI GATnn'nnATC

Cuts at:      6    1230    3267       6 
   Size:       1224    2037     455

  Fragments arranged by size: 

                2037    1224     455
 
BsaHI Gr'CG_yC

Cuts at:   1147    1395    2097    3593    1147 
   Size:        248     702    1496    1270

  Fragments arranged by size: 

                1496    1270     702     248
 
BsaWI w'CCGG_w

Cuts at:    905    1426    2104    2515    2662    2936     905 
   Size:        521     678     411     147     274    1685

  Fragments arranged by size: 

                1685     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    107     137     107 
   Size:         30    3686
 
BseYI C'CCAG_C

Cuts at:   1141    1899    2564    2972    1141 
   Size:        758     665     408    1885

  Fragments arranged by size: 

                1885     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104     384    1258    1304    2534     104 
   Size:        280     874      46    1230    1286

  Fragments arranged by size: 

                1286    1230     874     280      46
 
BsiHKAI G_wGCw'C

Cuts at:    429     730    1508    1698    2558     429 
   Size:        301     778     190     860    1587

  Fragments arranged by size: 

                1587     860     778     301     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    296     296 
   Size:       3716
 
Bsp1286I G_dGCh'C

Cuts at:    429     730    1341    1434    1508    1698    1760    2558     429 
   Size:        301     611      93      74     190      62     798    1587

  Fragments arranged by size: 

                1587     798     611     301     190      93      74      62
            
 
BspHI T'CATG_A

Cuts at:    654    2148     654 
   Size:       1494    2222
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    529     766     980    1282    1663     529 
   Size:        237     214     302     381    2582

  Fragments arranged by size: 

                2582     381     302     237     214
 
BsrBI CCG'CTC

Cuts at:     50    2008    2062    3546    3711      50 
   Size:       1958      54    1484     165      55

  Fragments arranged by size: 

                1958    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1628    1628 
   Size:       3716
 
BsrFI r'CCGG_y

Cuts at:   1714    1895    1714 
   Size:        181    3535
 
BssSI C'ACGA_G

Cuts at:   1987    2695    1987 
   Size:        708    3008
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1093    1093 
   Size:       3716
 
BstBI TT'CG_AA

Cuts at:    624    2077     624 
   Size:       1453    2263
 
BstYI r'GATC_y

Cuts at:      1     649     741    1208    1231    1566    1812    2118    2130 
   Size:        648      92     467      23     335     246     306      12

Cuts at:   2130    2216    3268       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1052     648     467     449     335     306     246      92
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    258     384     258 
   Size:        126    3590
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1666    1847    2010    1666 
   Size:        181     163    3372

  Fragments arranged by size: 

                3372     181     163
 
BtsI GCAGTG_nn'

Cuts at:    959     959 
   Size:       3716
 
DraI TTT'AAA

Cuts at:    518     715     518 
   Size:        197    3519
 
DrdI GACnn_nn'nnGTC

Cuts at:   1422    2766    1422 
   Size:       1344    2372
 
EaeI y'GGCC_r

Cuts at:    381    1301    1475    1866    1893    3238     381 
   Size:        920     174     391      27    1345     859

  Fragments arranged by size: 

                1345     920     859     391     174      27
 
EagI C'GGCC_G

Cuts at:    381    1301     381 
   Size:        920    2796
 
EarI CTCTTCn'nnn_

Cuts at:   1739    1949    1739 
   Size:        210    3506
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    437    1924    2666    2812    3526     437 
   Size:       1487     742     146     714     627

  Fragments arranged by size: 

                1487     742     714     627     146
 
EcoICRI GAG'CTC

Cuts at:    427     728     427 
   Size:        301    3415
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    164    1541    1973    2326    2988     164 
   Size:       1377     432     353     662     892

  Fragments arranged by size: 

                1377     892     662     432     353
 
EcoO109I rG'GnC_Cy

Cuts at:    294     855     294 
   Size:        561    3155
 
EcoRI G'AATT_C

Cuts at:    720     720 
   Size:       3716
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1735    1735 
   Size:       3716
 
FspI TGC'GCA

Cuts at:   1497    1497 
   Size:       3716
 
HaeII r_GCGC'y

Cuts at:    230    1150    1398    2628    3399     230 
   Size:        920     248    1230     771     547

  Fragments arranged by size: 

                1230     920     771     547     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    464     496    1558    1588    1590    1620     464 
   Size:         32    1062      30       2      30    2560

  Fragments arranged by size: 

                2560    1062      32      30      30       2
 
HincII GTy'rAC

Cuts at:    755     755 
   Size:       3716
 
KasI G'GCGC_C

Cuts at:   1146    1394    1146 
   Size:        248    3468
 
KpnI G_GTAC'C

Cuts at:    354     736     354 
   Size:        382    3334
 
MluI A'CGCG_T

Cuts at:   3149    3149 
   Size:       3716
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2475    2659    2475 
   Size:        184    3532
 
MscI TGG'CCA

Cuts at:   1477    1477 
   Size:       3716
 
MslI CAynn'nnrTG

Cuts at:    163     530    1705    1832     163 
   Size:        367    1175     127    2047

  Fragments arranged by size: 

                2047    1175     367     127
 
MspA1I CmG'CkG

Cuts at:    260     827    1141    1501    2285    2530     260 
   Size:        567     314     360     784     245    1446

  Fragments arranged by size: 

                1446     784     567     360     314     245
 
NaeI GCC'GGC

Cuts at:   1897    1897 
   Size:       3716
 
NarI GG'CG_CC

Cuts at:   1147    1395    1147 
   Size:        248    3468
 
NgoMIV G'CCGG_C

Cuts at:   1895    1895 
   Size:       3716
 
NruI TCG'CGA

Cuts at:    329     329 
   Size:       3716
 
NspI r_CATG'y

Cuts at:    531     769    1269    2872     531 
   Size:        238     500    1603    1375

  Fragments arranged by size: 

                1603    1375     500     238
 
PciI A'CATG_T

Cuts at:   2868    2868 
   Size:       3716
 
PmeI GTTT'AAAC

Cuts at:    715     715 
   Size:       3716
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    196     228    2162    2194     196 
   Size:         32    1934      32    1718

  Fragments arranged by size: 

                1934    1718      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    294     294 
   Size:       3716
 
PstI C_TGCA'G

Cuts at:    763    1448     763 
   Size:        685    3031
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3716
 
PvuII CAG'CTG

Cuts at:   1141    1501    1141 
   Size:        360    3356
 
RsrII CG'GwC_CG

Cuts at:   1911    1911 
   Size:       3716
 
SacI G_AGCT'C

Cuts at:    429     730     429 
   Size:        301    3415
 
SacII CC_GC'GG

Cuts at:    261     261 
   Size:       3716
 
SalI G'TCGA_C

Cuts at:    753     753 
   Size:       3716
 
SapI GCTCTTCn'nnn_

Cuts at:   1739    1949    1739 
   Size:        210    3506
 
SbfI CC_TGCA'GG

Cuts at:    763     763 
   Size:       3716
 
ScaI AGT'ACT

Cuts at:   3233    3233 
   Size:       3716
 
SfcI C'TryA_G

Cuts at:     33     759    1444    2412    2603      33 
   Size:        726     685     968     191    1146

  Fragments arranged by size: 

                1146     968     726     685     191
 
SfoI GGC'GCC

Cuts at:   1148    1396    1148 
   Size:        248    3468
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3716
 
SmaI CCC'GGG

Cuts at:    738     738 
   Size:       3716
 
SmlI C'TyrA_G

Cuts at:    247     871    1786    2500    2762     247 
   Size:        624     915     714     262    1201

  Fragments arranged by size: 

                1201     915     714     624     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3716
 
SphI G_CATG'C

Cuts at:    769    1269     769 
   Size:        500    3216
 
StyI C'CwwG_G

Cuts at:    194     850    1204     194 
   Size:        656     354    2706

  Fragments arranged by size: 

                2706     656     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1299    1432    1891    2100    1299 
   Size:        133     459     209    2915

  Fragments arranged by size: 

                2915     459     209     133
 
TatI w'GTAC_w

Cuts at:   3231    3231 
   Size:       3716
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    107     139    3233    3265     107 
   Size:         32    3094      32     558

  Fragments arranged by size: 

                3094     558      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1513    1513 
   Size:       3716
 
XbaI T'CTAG_A

Cuts at:    747     747 
   Size:       3716
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    503    1211     503 
   Size:        708    3008
 
XmaI C'CCGG_G

Cuts at:    736     736 
   Size:       3716
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NruI
     NspI     PciI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AlfI     AloI     ApaI
     AscI    AvrII    BbvCI    BfrBI     BmtI   Bpu10I    BseRI     BsgI
    BsiWI     BsmI    BspEI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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