Restriction map for pF1KB4573
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        Alw44I      
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BseAI       
BseRI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrFI       BsrGI       
BssHII      Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        EclXI       Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT14I     
EcoT22I     FbaI        FseI        Hin1I       HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MluI        MluNI       MroI        MscI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacI        SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StyI        SwaI        XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AciI          951  GCGG                 CCGC(-3/-1)
AhdI          636  GACTATGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1855  AGCGCT               AGC^GCT
AspEI         636  GACTATGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1917  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1926  CCCGAG               C^[CT]CG[GA]G
AvaII         968  GGACC                G^G[AT]CC
AviII        1190  TGCGCA               TGC^GCA
BanI          954  GGCACC               G^G[CT][GA]CC
BanII         900  GAGCCC               G[GA]GC[CT]^C
BglI          902  GCCCAACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1704  AGATCT               A^GATCT
BsaI         1309  GAGACC               GGTCTC(1/5)
BsaWI        1850  ACCGGA               [AT]^CCGG[AT]
BsgI          287  GTGCAG               GTGCAG(16/14)
BsmI         1553  GAATGC               GAATGC(1/-1)
BsoBI        1926  CCCGAG               C^[CT]CG[GA]G
BspHI         516  TCATGA               T^CATGA
BsrBI         949  GAGCGG               CCGCTC(-3/-3)
BssSI         995  CTCGTG               CACGAG(-5/-1)
BstBI         842  TTCGAA               TT^CGAA
BstXI         904  CCAACAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I      636  GACTATGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1855  AGCGCT               AGC^GCT
EcoRV        1897  GATATC               GAT^ATC
FauI          951  GCGGG                CCCGC(4/6)
FspI         1190  TGCGCA               TGC^GCA
HindIII       442  AAGCTT               A^AGCTT
MfeI         1253  CAATTG               C^AATTG
MspA1I         75  CAGCTG               C[AC]G^C[GT]G
MunI         1253  CAATTG               C^AATTG
NspV          842  TTCGAA               TT^CGAA
PflFI        1917  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         958  CCAGTATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         967  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI         457  GACATCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII          75  CAGCTG               CAG^CTG
RcaI          516  TCATGA               T^CATGA
SfcI          525  CTGTAG               C^T[GA][CT]AG
SfuI          842  TTCGAA               TT^CGAA
SmlI          256  CTCAAG               C^T[CT][GA]AG
SphI         1377  GCATGC               GCATG^C
Tth111I      1917  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        958  CCAGTATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          803  CCACCCTGCCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          538  GTAC                 GT^AC
             1230  GTAC                
AlwNI         959  CAGTATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1801  CAGATCCTG           
Asp700I       370  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              429  GAAGACGTTC          
AspHI           4  GAGCAC               G[AT]GC[AT]^C
              520  GAGCAC              
BcnI          120  CCGGG                CC^[GC]GG
             1346  CCCGG               
BsiHKAI         4  GAGCAC               G[AT]GC[AT]^C
              520  GAGCAC              
BsmAI        1309  GAGAC                GTCTC(1/5)
             1400  GAGAC               
BspMI        1295  ACCTGC               ACCTGC(4/8)
             1500  GCAGGT              
DraII         266  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              967  GGGACCC             
EarI         1743  GAAGAG               CTCTTC(1/4)
             1780  GAAGAG              
EcoO109I      266  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              967  GGGACCC             
HaeII         116  AGCGCC               [GA]GCGC^[CT]
             1855  AGCGCT              
HgaI          355  GCGTC                GACGC(5/10)
              642  GCGTC               
HincII        768  GTCGAC               GT[CT]^[GA]AC
             1138  GTCGAC              
HindII        768  GTCGAC               GT[CT]^[GA]AC
             1138  GTCGAC              
Ksp632I      1743  GAAGAG               CTCTTC(1/4)
             1780  GAAGAG              
MaeII         433  ACGT                 A^CGT
             1921  ACGT                
MseI          719  TTAA                 T^TAA
             1438  TTAA                
NciI          120  CCGGG                CC^[GC]GG
             1346  CCCGG               
PleI          614  GAGTC                GAGTC(4/5)
              655  GAGTC               
RsaI          538  GTAC                 GT^AC
             1230  GTAC                
SalI          768  GTCGAC               G^TCGAC
             1138  GTCGAC              
Tru9I         719  TTAA                 T^TAA
             1438  TTAA                
XmnI          370  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              429  GAAGACGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccI        AcsI        AluI        AlwI        ApoI        
BbsI        BbvI        BmyI        BpmI        BpuAI       
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
Sau3AI      Sau96I      ScrFI       SfaNI       StuI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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