Restriction map for pF1KB4573
 (Circular) (Six-base) MAPSORT of: pF1KB4573.seq  Check: 813  from: 1  to: 5057



pF1KB4573  5057 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1412    1412 
   Size:       5057
 
AccI GT'mk_AC

Cuts at:    877    1247    1700    2095     877 
   Size:        370     453     395    3839

  Fragments arranged by size: 

                3839     453     395     370
 
Acc65I G'GTAC_C

Cuts at:   2073    2073 
   Size:       5057
 
AclI AA'CG_TT

Cuts at:   4897    4897 
   Size:       5057
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    568     869     962    1260    2882    3314    3667    4329     568 
   Size:        301      93     298    1622     432     353     662    1296

  Fragments arranged by size: 

                1622    1296     662     432     353     301     298      93
            
 
AfeI AGC'GCT

Cuts at:   1965    1965 
   Size:       5057
 
AflIII A'CryG_T

Cuts at:   2409    4209    4490    2409 
   Size:       1800     281    2976

  Fragments arranged by size: 

                2976    1800     281
 
AhdI GACnn_n'nnGTC

Cuts at:    749     749 
   Size:       5057
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    931     931 
   Size:       5057
 
AlwNI CAG_nnn'CTG

Cuts at:   1072    1914    2362    3800    1072 
   Size:        842     448    1438    2329

  Fragments arranged by size: 

                2329    1438     842     448
 
ApaLI G'TGCA_C

Cuts at:   3895    3895 
   Size:       5057
 
ApoI r'AATT_y

Cuts at:    577     619     699    2061    4410    4666     577 
   Size:         42      80    1362    2349     256     968

  Fragments arranged by size: 

                2349    1362     968     256      80      42
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5057
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5057
 
AvaI C'yCGr_G

Cuts at:   2034    2077    2034 
   Size:         43    5014
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    629     662     629 
   Size:         33    5024
 
BamHI G'GATC_C

Cuts at:   2082    2082 
   Size:       5057
 
BanI G'GyrC_C

Cuts at:   1062    2073    2487    2735    2770    1062 
   Size:       1011     414     248      35    3349

  Fragments arranged by size: 

                3349    1011     414     248      35
 
BanII G_rGCy'C

Cuts at:   1012    2071    3101    1012 
   Size:       1059    1030    2968

  Fragments arranged by size: 

                2968    1059    1030
 
BbeI G_GCGC'C

Cuts at:   2491    2739    2491 
   Size:        248    4809
 
BbsI GAAGACnn'nnnn_

Cuts at:    139     544    1910    2040     139 
   Size:        405    1366     130    3156

  Fragments arranged by size: 

                3156    1366     405     130
 
BciVI GTATCCnnnnn_n'

Cuts at:   2955    4011    5057    2955 
   Size:       1056    1046    2955

  Fragments arranged by size: 

                2955    1056    1046
 
BclI T'GATC_A

Cuts at:   2577    2577 
   Size:       5057
 
BglI GCCn_nnn'nGGC

Cuts at:   1016    1016 
   Size:       5057
 
BglII A'GATC_T

Cuts at:   1812    2572    4609    1812 
   Size:        760    2037    2260

  Fragments arranged by size: 

                2260    2037     760
 
BlpI GC'TnA_GC

Cuts at:   2169    2169 
   Size:       5057
 
Bme1580I G_kGCm'C

Cuts at:   1065    2682    2775    3899    1065 
   Size:       1617      93    1124    2223

  Fragments arranged by size: 

                2223    1617    1124      93
 
BmgBI CAC'GTC

Cuts at:   2030    2030 
   Size:       5057
 
BmrI ACTGGGnnnn_n'

Cuts at:   1365    2269    2350    2685    4829    1365 
   Size:        904      81     335    2144    1593

  Fragments arranged by size: 

                2144    1593     904     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1619    3424    1619 
   Size:       1805    3252
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    165     687     819    1614     165 
   Size:        522     132     795    3608

  Fragments arranged by size: 

                3608     795     522     132
 
Bpu10I CC'TnA_GC

Cuts at:   1466    1466 
   Size:       5057
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    349    2233    3112    3826    4124     349 
   Size:       1884     879     714     298    1282

  Fragments arranged by size: 

                1884    1282     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1411    4761    1411 
   Size:       3350    1707
 
BsaAI yAC'GTr

Cuts at:   2410    2410 
   Size:       5057
 
BsaBI GATnn'nnATC

Cuts at:      6    2571    4608       6 
   Size:       2565    2037     455

  Fragments arranged by size: 

                2565    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2488    2736    3438    4934    2488 
   Size:        248     702    1496    2611

  Fragments arranged by size: 

                2611    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1958    2246    2767    3445    3856    4003    4277    1958 
   Size:        288     521     678     411     147     274    2738

  Fragments arranged by size: 

                2738     678     521     411     288     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1408    1438    1408 
   Size:         30    5027
 
BseYI C'CCAG_C

Cuts at:    726    2482    3240    3905    4313     726 
   Size:       1756     758     665     408    1470

  Fragments arranged by size: 

                1756    1470     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    416     416 
   Size:       5057
 
BsiEI CG_ry'CG

Cuts at:    104    2599    2645    3875     104 
   Size:       2495      46    1230    1286

  Fragments arranged by size: 

                2495    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    116     632    2071    2849    3039    3899     116 
   Size:        516    1439     778     190     860    1274

  Fragments arranged by size: 

                1439    1274     860     778     516     190
 
BsmI GAATG_Cn'

Cuts at:   1667    1667 
   Size:       5057
 
Bsp1286I G_dGCh'C

Cuts at:    116     632    1012    1065    2071    2682    2775    2849    3039 
   Size:        516     380      53    1006     611      93      74     190

Cuts at:   3039    3101    3899     116 
   Size:         62     798    1274

  Fragments arranged by size: 

                1274    1006     798     611     516     380     190      93
                  74      62      53
 
BspHI T'CATG_A

Cuts at:    624    3489     624 
   Size:       2865    2192
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1412    1599    2107    2321    2623    3004    1412 
   Size:        187     508     214     302     381    3465

  Fragments arranged by size: 

                3465     508     381     302     214     187
 
BsrBI CCG'CTC

Cuts at:     50    1059    3349    3403    4887    5052      50 
   Size:       1009    2290      54    1484     165      55

  Fragments arranged by size: 

                2290    1484    1009     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    150    1448    1853    2969     150 
   Size:       1298     405    1116    2238

  Fragments arranged by size: 

                2238    1298    1116     405
 
BsrFI r'CCGG_y

Cuts at:   3055    3236    3055 
   Size:        181    4876
 
BssSI C'ACGA_G

Cuts at:   1103    3328    4036    1103 
   Size:       2225     708    2124

  Fragments arranged by size: 

                2225    2124     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2434    2434 
   Size:       5057
 
BstBI TT'CG_AA

Cuts at:    951    3418     951 
   Size:       2467    2590
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1019    1019 
   Size:       5057
 
BstYI r'GATC_y

Cuts at:      1     255    1232    1812    1910    2082    2549    2572    2907 
   Size:        254     977     580      98     172     467      23     335

Cuts at:   2907    3153    3459    3471    3557    4609       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                1052     977     580     467     449     335     306     254
                 246     172      98      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       5057
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3007    3188    3351    3007 
   Size:        181     163    4713

  Fragments arranged by size: 

                4713     181     163
 
BtsI GCAGTG_nn'

Cuts at:    404    2300     404 
   Size:       1896    3161
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1152    1187    1152 
   Size:         35    5022
 
DraI TTT'AAA

Cuts at:   2056    2056 
   Size:       5057
 
DrdI GACnn_nn'nnGTC

Cuts at:   2763    4107    2763 
   Size:       1344    3713
 
EaeI y'GGCC_r

Cuts at:   2642    2816    3207    3234    4579    2642 
   Size:        174     391      27    1345    3120

  Fragments arranged by size: 

                3120    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2642    2642 
   Size:       5057
 
EarI CTCTTCn'nnn_

Cuts at:   1846    1883    3080    3290    1846 
   Size:         37    1197     210    3613

  Fragments arranged by size: 

                3613    1197     210      37
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3265    4007    4153    4867    3265 
   Size:        742     146     714    3455

  Fragments arranged by size: 

                3455     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2069    2069 
   Size:       5057
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    165     568     687     819     869     962    1260    1614    2882 
   Size:        403     119     132      50      93     298     354    1268

Cuts at:   2882    3314    3667    4329     165 
   Size:        432     353     662     893

  Fragments arranged by size: 

                1268     893     662     432     403     354     353     298
                 132     119      93      50
 
EcoO109I rG'GnC_Cy

Cuts at:    375    1076    2196     375 
   Size:        701    1120    3236

  Fragments arranged by size: 

                3236    1120     701
 
EcoRI G'AATT_C

Cuts at:   2061    2061 
   Size:       5057
 
EcoRV GAT'ATC

Cuts at:   2007    2007 
   Size:       5057
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    573    3076     573 
   Size:       2503    2554
 
FspI TGC'GCA

Cuts at:   1300    2838    1300 
   Size:       1538    3519
 
HaeII r_GCGC'y

Cuts at:    228    1967    2491    2739    3969    4740     228 
   Size:       1739     524     248    1230     771     545

  Fragments arranged by size: 

                1739    1230     771     545     524     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    448     480     735     767    1501    1533    1687    1719    1760 
   Size:         32     255      32     734      32     154      32      41

Cuts at:   1760    1792    2899    2929    2931    2961     448 
   Size:         32    1107      30       2      30    2544

  Fragments arranged by size: 

                2544    1107     734     255     154      41      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:    878    1248    2096     878 
   Size:        370     848    3839

  Fragments arranged by size: 

                3839     848     370
 
HindIII A'AGCT_T

Cuts at:    550     550 
   Size:       5057
 
KasI G'GCGC_C

Cuts at:   2487    2735    2487 
   Size:        248    4809
 
KpnI G_GTAC'C

Cuts at:   2077    2077 
   Size:       5057
 
MfeI C'AATT_G

Cuts at:   1361    1361 
   Size:       5057
 
MluI A'CGCG_T

Cuts at:   4490    4490 
   Size:       5057
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1544    1673    1959    3816    4000    1544 
   Size:        129     286    1857     184    2601

  Fragments arranged by size: 

                2601    1857     286     184     129
 
MscI TGG'CCA

Cuts at:   2818    2818 
   Size:       5057
 
MslI CAynn'nnrTG

Cuts at:   1217    1406    1783    3046    3173    1217 
   Size:        189     377    1263     127    3101

  Fragments arranged by size: 

                3101    1263     377     189     127
 
MspA1I CmG'CkG

Cuts at:    185    2168    2482    2842    3626    3871     185 
   Size:       1983     314     360     784     245    1371

  Fragments arranged by size: 

                1983    1371     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   3238    3238 
   Size:       5057
 
NarI GG'CG_CC

Cuts at:   2488    2736    2488 
   Size:        248    4809
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       5057
 
NgoMIV G'CCGG_C

Cuts at:   3236    3236 
   Size:       5057
 
NspI r_CATG'y

Cuts at:   1315    1411    1489    2110    2610    4213    1315 
   Size:         96      78     621     500    1603    2159

  Fragments arranged by size: 

                2159    1603     621     500      96      78
 
PciI A'CATG_T

Cuts at:   4209    4209 
   Size:       5057
 
PflMI CCAn_nnn'nTGG

Cuts at:   1072    1072 
   Size:       5057
 
PfoI T'CCnGG_A

Cuts at:    497     772    1412     497 
   Size:        275     640    4142

  Fragments arranged by size: 

                4142     640     275
 
PmeI GTTT'AAAC

Cuts at:   2056    2056 
   Size:       5057
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3503    3535    3503 
   Size:         32    5025
 
PpuMI rG'GwC_Cy

Cuts at:   1076    1076 
   Size:       5057
 
PshAI GACnn'nnGTC

Cuts at:    569     569 
   Size:       5057
 
PsiI TTA'TAA

Cuts at:   1199    1199 
   Size:       5057
 
PstI C_TGCA'G

Cuts at:   2104    2789    2104 
   Size:        685    4372
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5057
 
PvuII CAG'CTG

Cuts at:    185    2482    2842     185 
   Size:       2297     360    2400

  Fragments arranged by size: 

                2400    2297     360
 
RsrII CG'GwC_CG

Cuts at:   3252    3252 
   Size:       5057
 
SacI G_AGCT'C

Cuts at:   2071    2071 
   Size:       5057
 
SalI G'TCGA_C

Cuts at:    876    1246    2094     876 
   Size:        370     848    3839

  Fragments arranged by size: 

                3839     848     370
 
SanDI GG'GwC_CC

Cuts at:   1076    1076 
   Size:       5057
 
SapI GCTCTTCn'nnn_

Cuts at:   3080    3290    3080 
   Size:        210    4847
 
SbfI CC_TGCA'GG

Cuts at:   2104    2104 
   Size:       5057
 
ScaI AGT'ACT

Cuts at:   4574    4574 
   Size:       5057
 
SfcI C'TryA_G

Cuts at:     33     633    2100    2785    3753    3944      33 
   Size:        600    1467     685     968     191    1146

  Fragments arranged by size: 

                1467    1146     968     685     600     191
 
SfoI GGC'GCC

Cuts at:   2489    2737    2489 
   Size:        248    4809
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5057
 
SmaI CCC'GGG

Cuts at:   2079    2079 
   Size:       5057
 
SmlI C'TyrA_G

Cuts at:    364    2212    3127    3841    4103     364 
   Size:       1848     915     714     262    1318

  Fragments arranged by size: 

                1848    1318     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5057
 
SphI G_CATG'C

Cuts at:   1489    2110    2610    1489 
   Size:        621     500    3936

  Fragments arranged by size: 

                3936     621     500
 
StuI AGG'CCT

Cuts at:   1101    1273    1896    1101 
   Size:        172     623    4262

  Fragments arranged by size: 

                4262     623     172
 
StyI C'CwwG_G

Cuts at:    107    2191    2545     107 
   Size:       2084     354    2619

  Fragments arranged by size: 

                2619    2084     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2640    2773    3232    3441    2640 
   Size:        133     459     209    4256

  Fragments arranged by size: 

                4256     459     209     133
 
TatI w'GTAC_w

Cuts at:   4572    4572 
   Size:       5057
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    822     854     878     910    4574    4606     822 
   Size:         32      24      32    3664      32    1273

  Fragments arranged by size: 

                3664    1273      32      32      32      24
 
Tth111I GACn'n_nGTC

Cuts at:   2028    2854    2028 
   Size:        826    4231
 
XbaI T'CTAG_A

Cuts at:   2088    2088 
   Size:       5057
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    918    2552     918 
   Size:       1634    3423
 
XmaI C'CCGG_G

Cuts at:   2077    2077 
   Size:       5057
 
XmnI GAAnn'nnTTC

Cuts at:    482     541     482 
   Size:         59    4998
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AhdI
     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI
    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AgeI     AleI     AloI     ApaI     AscI    AvrII
    BbvCI     BcgI    BfrBI     BmtI    BseRI    BsiWI    BsmBI    BspEI
    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI   DraIII    EcoNI
     FseI    FspAI     HpaI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PmlI   PspOMI    PspXI     PsrI    SacII    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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