Restriction map for pF1KB4524
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AccI        AcyI        AflII       
AflIII      AgeI        Alw44I      Aor51HI     ApaLI       
AscI        Asp718I     AviII       AvrII       BalI        
BbeI        BbrPI       BciVI       BfrI        BglI        
BlnI        BsaBI       BsaHI       BsiWI       BsmBI       
BspDI       BspLU11I    BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
CfoI        ClaI        CpoI        DraI        DraIII      
DrdI        Eco47III    EcoO65I     EcoRV       FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      KasI        KpnI        MamI        MfeI        
MluI        MluNI       MscI        MunI        NarI        
NotI        NruI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PvuI        RsrII       SalI        SapI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SpeI        Sse8387I    SspI        StuI        SwaI        
Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

AccII          34  CGCG                 CG^CG
AccIII       2336  TCCGGA               T^CCGGA
AclI          878  AACGTT               AA^CGTT
AhdI         3025  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1972  CCTGAGG              CC^T[ACGT]AGG
AseI         1389  ATTAAT               AT^TAAT
AsnI         1389  ATTAAT               AT^TAAT
AspEI        3025  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2658  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           41  CCCGGG               C^[CT]CG[GA]G
BamHI         744  GGATCC               G^GATCC
BanI         1364  GGCACC               G^G[CT][GA]CC
BbsI         3602  GTCTTC               GAAGAC(2/6)
BbvCI        2365  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1041  GCAGGACCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1542  AGATCT               A^GATCT
BpuAI        3602  GTCTTC               GAAGAC(2/6)
BsaAI        2900  TACGTA               [CT]AC^GT[GA]
BsaI         3334  GAGACC               GGTCTC(1/5)
BsaWI        2336  TCCGGA               [AT]^CCGG[AT]
BseAI        2336  TCCGGA               T^CCGGA
BsiEI          30  CGGCCG               CG[GA][CT]^CG
BsoBI          41  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      386  TGTACA               T^GTACA
BspEI        2336  TCCGGA               T^CCGGA
BspHI         594  TCATGA               T^CATGA
BspMI        1797  ACCTGC               ACCTGC(4/8)
BsrBI        3523  GAGCGG               CCGCTC(-3/-3)
BsrFI        1361  GCCGGC               [GA]^CCGG[CT]
BsrGI         386  TGTACA               T^GTACA
BstUI          34  CGCG                 CG^CG
Bsu36I       1972  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1361  GCCGGC               [GA]^CCGG[CT]
EagI           30  CGGCCG               C^GGCCG
Eam1105I     3025  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          30  CGGCCG               C^GGCCG
Eco52I         30  CGGCCG               C^GGCCG
Eco81I       1972  CCTGAGG              CC^T[ACGT]AGG
EcoRI        2096  GAATTC               G^AATTC
EcoT22I      3319  ATGCAT               ATGCA^T
HincII       1473  GTTAAC               GT[CT]^[GA]AC
HindII       1473  GTTAAC               GT[CT]^[GA]AC
HpaI         1473  GTTAAC               GTT^AAC
KspI           33  CCGCGG               CCGC^GG
MroI         2336  TCCGGA               T^CCGGA
MvnI           34  CGCG                 CG^CG
NaeI         1361  GCCGGC               GCC^GGC
NdeI          221  CATATG               CA^TATG
NgoMIV       1361  GCCGGC               G^CCGGC
NheI         3696  GCTAGC               G^CTAGC
NsiI         3319  ATGCAT               ATGCA^T
PflFI        2658  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        1389  ATTAAT               AT^TAAT
Psp1406I      878  AACGTT               AA^CGTT
RcaI          594  TCATGA               T^CATGA
SacI          420  GAGCTC               GAGCT^C
SacII          33  CCGCGG               CCGC^GG
SexAI        1764  ACCTGGT              A^CC[AT]GGT
SmaI           41  CCCGGG               CCC^GGG
SnaBI        2900  TACGTA               TAC^GTA
SphI         2603  GCATGC               GCATG^C
SspBI         386  TGTACA               T^GTACA
Tth111I      2658  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3606  TCTAGA               T^CTAGA
XcmI         1673  CCAAGAAAGAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          41  CCCGGG               C^CCGGG
XmaI           41  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

ApaI           25  GGGCCC               GGGCC^C
             3649  GGGCCC              
Asp700I       206  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1174  GAAGTCTTTC          
AspHI         420  GAGCTC               G[AT]GC[AT]^C
             1736  GTGCTC              
BclI          950  TGATCA               T^GATCA
             1395  TGATCA              
BlpI          483  GCTCAGC              GC^T[ACGT]AGC
              778  GCTAAGC             
Bpu1102I      483  GCTCAGC              GC^T[ACGT]AGC
              778  GCTAAGC             
BsiHKAI       420  GAGCTC               G[AT]GC[AT]^C
             1736  GTGCTC              
BsrDI         248  CATTGC               GCAATG(2/0)
              844  GCAATG              
BstXI        2354  CCAAGCTTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3791  CCAAGGAGTTGG        
CelII         483  GCTCAGC              GC^T[ACGT]AGC
              778  GCTAAGC             
EaeI           30  CGGCCG               [CT]^GGCC[GA]
             3804  TGGCCG              
FbaI          950  TGATCA               T^GATCA
             1395  TGATCA              
NcoI          740  CCATGG               C^CATGG
             3725  CCATGG              
NspI         2603  GCATGC               [GA]CATG^[CT]
             2887  GCATGT              
ScaI         1286  AGTACT               AGT^ACT
             3359  AGTACT              
SmlI         3103  CTCAAG               C^T[CT][GA]AG
             3781  CTTGAG              
TaqI         1050  TCGA                 T^CGA
             3021  TCGA                
TthHB8I      1050  TCGA                 T^CGA
             3021  TCGA                
XmnI          206  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1174  GAAGTCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
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