Restriction map for pF1KB4445
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmI        BsoBI       Bsp1286I    
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      Cac8I       CelII       
Cfr10I      CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MseI        MslI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        TfiI        Tru9I       Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

AciI          308  CCGC                 CCGC(-3/-1)
AfaI          427  GTAC                 GT^AC
AvaII         185  GGACC                G^G[AT]CC
BcnI          198  CCCGG                CC^[GC]GG
BsaAI         322  CACGTA               [CT]AC^GT[GA]
Bsp1407I      426  TGTACA               T^GTACA
BspDI         391  ATCGAT               AT^CGAT
BsrGI         426  TGTACA               T^GTACA
BstXI          89  CCAAGGAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          305  GCGC                 GCG^C
Cfr13I        185  GGACC                G^G[ACGT]CC
ClaI          391  ATCGAT               AT^CGAT
DdeI           17  CTGAG                C^T[ACGT]AG
EarI          418  GAAGAG               CTCTTC(1/4)
EcoT14I        89  CCAAGG               C^C[AT][AT]GG
HaeII         304  AGCGCC               [GA]GCGC^[CT]
HapII         199  CCGG                 C^CGG
HgaI          318  GCGTC                GACGC(5/10)
HhaI          305  GCGC                 GCG^C
HinP1I        305  GCGC                 G^CGC
HpaII         199  CCGG                 C^CGG
HphI           83  TCACC                GGTGA(8/7)
Ksp632I       418  GAAGAG               CTCTTC(1/4)
MaeII         323  ACGT                 A^CGT
MaeIII        320  GTCAC                ^GT[ACGT]AC
MboII         418  GAAGA                GAAGA(8/7)
MspA1I         10  CAGCTG               C[AC]G^C[GT]G
MspI          199  CCGG                 C^CGG
MwoI          296  GCTACATCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          198  CCCGG                CC^[GC]GG
NlaIII        213  CATG                 CATG^
NlaIV         184  GGGACC               GG[ACGT]^[ACGT]CC
PstI          145  CTGCAG               CTGCA^G
PvuII          10  CAGCTG               CAG^CTG
RsaI          427  GTAC                 GT^AC
Sau96I        185  GGACC                G^G[ACGT]CC
SfaNI         169  GATGC                GCATC(5/9)
SfcI          145  CTGCAG               C^T[GA][CT]AG
SspBI         426  TGTACA               T^GTACA
StuI           44  AGGCCT               AGG^CCT
StyI           89  CCAAGG               C^C[AT][AT]GG
TaqI          392  TCGA                 T^CGA
Tsp45I        320  GTCAC                ^GT[GC]AC
Tsp509I       412  AATT                 ^AATT
TthHB8I       392  TCGA                 T^CGA
XcmI           86  CCACCAAGGAGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AlwI          113  GATCC                GGATC(4/5)
              255  GATCC               
BalI          218  TGGCCA               TGG^CCA
              402  TGGCCA              
BbvI          144  GCTGC                GCAGC(8/12)
              315  GCTGC               
BclI          155  TGATCA               T^GATCA
              374  TGATCA              
BstYI         112  AGATCC               [GA]^GATC[CT]
              254  AGATCC              
EaeI          218  TGGCCA               [CT]^GGCC[GA]
              402  TGGCCA              
FbaI          155  TGATCA               T^GATCA
              374  TGATCA              
MflI          112  AGATCC               [GA]^GATC[CT]
              254  AGATCC              
MluNI         218  TGGCCA               TGG^CCA
              402  TGGCCA              
MscI          218  TGGCCA               TGG^CCA
              402  TGGCCA              
TseI          144  GCTGC                G^C[AT]GC
              315  GCTGC               
XhoII         112  AGATCC               [GA]^GATC[CT]
              254  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AluI        BsaJI       BseRI       BsmFI       BssKI       
BstF5I      BstNI       DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      ItaI        MboI        MnlI        
MvaI        NdeII       Sau3AI      ScrFI       TspRI       
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