Restriction map for pF1KB4430
 (Linear) (Six-base) MAPSORT of: KIBB4430.seq  Check: 4637  from: 1  to: 1701



KIBB4430.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1242    1701 
   Size:       1242     459
 
AccI GT'mk_AC

Cuts at:      0    1340    1701 
   Size:       1340     361
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     785     808     916    1596    1694    1701 
   Size:        785      23     108     680      98       7

  Fragments arranged by size: 

                 785     680     108      98      23       7
 
AflIII A'CryG_T

Cuts at:      0      52     688    1469    1701 
   Size:         52     636     781     232

  Fragments arranged by size: 

                 781     636     232      52
 
AleI CACnn'nnGTG

Cuts at:      0     147     745    1445    1701 
   Size:        147     598     700     256

  Fragments arranged by size: 

                 700     598     256     147
 
AlwNI CAG_nnn'CTG

Cuts at:      0     588     921    1508    1701 
   Size:        588     333     587     193

  Fragments arranged by size: 

                 588     587     333     193
 
ApaLI G'TGCA_C

Cuts at:      0    1126    1701 
   Size:       1126     575
 
ApoI r'AATT_y

Cuts at:      0     154    1302    1496    1701 
   Size:        154    1148     194     205

  Fragments arranged by size: 

                1148     205     194     154
 
AvaI C'yCGr_G

Cuts at:      0    1064    1641    1701 
   Size:       1064     577      60

  Fragments arranged by size: 

                1064     577      60
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     988    1021    1701 
   Size:        988      33     680

  Fragments arranged by size: 

                 988     680      33
 
BanII G_rGCy'C

Cuts at:      0    1147    1701 
   Size:       1147     554
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     868     946    1682    1701 
   Size:        868      78     736      19

  Fragments arranged by size: 

                 868     736      78      19
 
BbvCI CC'TCA_GC

Cuts at:      0     767     929    1701 
   Size:        767     162     772

  Fragments arranged by size: 

                 772     767     162
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     628     662    1041    1075    1701 
   Size:        628      34     379      34     626

  Fragments arranged by size: 

                 628     626     379      34      34
 
BclI T'GATC_A

Cuts at:      0     968    1089    1701 
   Size:        968     121     612

  Fragments arranged by size: 

                 968     612     121
 
BglII A'GATC_T

Cuts at:      0     830    1701 
   Size:        830     871
 
Bme1580I G_kGCm'C

Cuts at:      0    1130    1701 
   Size:       1130     571
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     535    1587    1701 
   Size:        535    1052     114

  Fragments arranged by size: 

                1052     535     114
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     870    1701 
   Size:        870     831
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     898    1262    1642    1701 
   Size:        898     364     380      59

  Fragments arranged by size: 

                 898     380     364      59
 
Bpu10I CC'TnA_GC

Cuts at:      0      16     767     929    1701 
   Size:         16     751     162     772

  Fragments arranged by size: 

                 772     751     162      16
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1123    1701 
   Size:       1123     578
 
BsaAI yAC'GTr

Cuts at:      0     689    1701 
   Size:        689    1012
 
BsaBI GATnn'nnATC

Cuts at:      0    1120    1701 
   Size:       1120     581
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     667     697    1701 
   Size:        667      30    1004

  Fragments arranged by size: 

                1004     667      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     859     946    1662    1675    1701 
   Size:        859      87     716      13      26

  Fragments arranged by size: 

                 859     716      87      26      13
 
BseYI C'CCAG_C

Cuts at:      0    1028    1047    1501    1559    1701 
   Size:       1028      19     454      58     142

  Fragments arranged by size: 

                1028     454     142      58      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     149    1701 
   Size:         19     130    1552

  Fragments arranged by size: 

                1552     130      19
 
BsiHKAI G_wGCw'C

Cuts at:      0     644    1130    1147    1361    1442    1701 
   Size:        644     486      17     214      81     259

  Fragments arranged by size: 

                 644     486     259     214      81      17
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1537    1588    1701 
   Size:       1537      51     113

  Fragments arranged by size: 

                1537     113      51
 
Bsp1286I G_dGCh'C

Cuts at:      0     644    1130    1147    1361    1442    1701 
   Size:        644     486      17     214      81     259

  Fragments arranged by size: 

                 644     486     259     214      81      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1046    1183    1242    1701 
   Size:       1046     137      59     459

  Fragments arranged by size: 

                1046     459     137      59
 
BsrBI CCG'CTC

Cuts at:      0     671     936    1701 
   Size:        671     265     765

  Fragments arranged by size: 

                 765     671     265
 
BsrDI GCAATG_nn'

Cuts at:      0     435    1701 
   Size:        435    1266
 
BsrFI r'CCGG_y

Cuts at:      0     575     607    1701 
   Size:        575      32    1094

  Fragments arranged by size: 

                1094     575      32
 
BssSI C'ACGA_G

Cuts at:      0    1156    1701 
   Size:       1156     545
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     657     735    1353    1701 
   Size:        657      78     618     348

  Fragments arranged by size: 

                 657     618     348      78
 
BstYI r'GATC_y

Cuts at:      0     830    1701 
   Size:        830     871
 
BtgI C'CryG_G

Cuts at:      0    1346    1701 
   Size:       1346     355
 
BtsI GCAGTG_nn'

Cuts at:      0    1394    1701 
   Size:       1394     307
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     109    1701 
   Size:        109    1592
 
EaeI y'GGCC_r

Cuts at:      0      28    1701 
   Size:         28    1673
 
EcoICRI GAG'CTC

Cuts at:      0    1145    1701 
   Size:       1145     556
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     785     808     898     916    1262    1596    1642    1694 
   Size:        785      23      90      18     346     334      46      52

Cuts at:   1694    1701 
   Size:          7

  Fragments arranged by size: 

                 785     346     334      90      52      46      23      18
                   7
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21    1035    1134    1701 
   Size:         21    1014      99     567

  Fragments arranged by size: 

                1014     567      99      21
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     894    1701 
   Size:        894     807
 
FspI TGC'GCA

Cuts at:      0    1042    1701 
   Size:       1042     659
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     655     667     687     699     865     897    1327    1359 
   Size:        655      12      20      12     166      32     430      32

Cuts at:   1359    1624    1656    1701 
   Size:        265      32      45

  Fragments arranged by size: 

                 655     430     265     166      45      32      32      32
                  20      12      12
 
HincII GTy'rAC

Cuts at:      0     573    1550    1701 
   Size:        573     977     151

  Fragments arranged by size: 

                 977     573     151
 
HpaI GTT'AAC

Cuts at:      0     573    1701 
   Size:        573    1128
 
MluI A'CGCG_T

Cuts at:      0      52    1701 
   Size:         52    1649
 
MslI CAynn'nnrTG

Cuts at:      0     147     745    1445    1531    1701 
   Size:        147     598     700      86     170

  Fragments arranged by size: 

                 700     598     170     147      86
 
MspA1I CmG'CkG

Cuts at:      0      33    1028    1701 
   Size:         33     995     673

  Fragments arranged by size: 

                 995     673      33
 
NcoI C'CATG_G

Cuts at:      0    1346    1701 
   Size:       1346     355
 
NspI r_CATG'y

Cuts at:      0    1021    1701 
   Size:       1021     680
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1508    1599    1701 
   Size:       1508      91     102

  Fragments arranged by size: 

                1508     102      91
 
PfoI T'CCnGG_A

Cuts at:      0     656    1294    1701 
   Size:        656     638     407

  Fragments arranged by size: 

                 656     638     407
 
PmlI CAC'GTG

Cuts at:      0     689    1701 
   Size:        689    1012
 
PpuMI rG'GwC_Cy

Cuts at:      0    1035    1134    1701 
   Size:       1035      99     567

  Fragments arranged by size: 

                1035     567      99
 
PshAI GACnn'nnGTC

Cuts at:      0    1336    1701 
   Size:       1336     365
 
PstI C_TGCA'G

Cuts at:      0     214    1701 
   Size:        214    1487
 
PvuII CAG'CTG

Cuts at:      0    1028    1701 
   Size:       1028     673
 
SacI G_AGCT'C

Cuts at:      0    1147    1701 
   Size:       1147     554
 
SfcI C'TryA_G

Cuts at:      0     210     411     997    1701 
   Size:        210     201     586     704

  Fragments arranged by size: 

                 704     586     210     201
 
SmaI CCC'GGG

Cuts at:      0    1066    1701 
   Size:       1066     635
 
SmlI C'TyrA_G

Cuts at:      0    1138    1701 
   Size:       1138     563
 
StyI C'CwwG_G

Cuts at:      0     986    1346    1427    1701 
   Size:        986     360      81     274

  Fragments arranged by size: 

                 986     360     274      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1348    1701 
   Size:       1348     353
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     453     485    1701 
   Size:        453      32    1216

  Fragments arranged by size: 

                1216     453      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1302    1701 
   Size:       1302     399
 
XmaI C'CCGG_G

Cuts at:      0    1064    1701 
   Size:       1064     637
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI    AlwNI    ApaLI     ApoI
     AvaI     BaeI    BanII     BbsI    BbvCI     BcgI     BclI    BglII
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI    BssSI    BstXI    BstYI     BtgI     BtsI
     DrdI     EaeI  EcoICRI  Eco57MI EcoO109I     FalI     FspI    Hin4I
   HincII     HpaI     MluI     MslI   MspA1I     NcoI     NspI    PflMI
     PfoI     PmlI    PpuMI    PshAI     PstI    PvuII     SacI     SfcI
     SmaI     SmlI     StyI    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII    BamHI     BanI
     BbeI    BciVI    BfrBI     BglI     BlpI    BmgBI     BmtI     BsaI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrGI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     EagI     EarI     EciI    EcoNI    EcoRI
    EcoRV     FseI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI
     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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