Restriction map for pF1KB4421
 (Linear) (Six-base) MAPSORT of: KIBB4421.seq  Check: 2747  from: 1  to: 1575



KIBB4421.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1568    1575 
   Size:       1568       7
 
AccI GT'mk_AC

Cuts at:      0    1175    1575 
   Size:       1175     400
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676    1145    1575 
   Size:        676     469     430

  Fragments arranged by size: 

                 676     469     430
 
AflIII A'CryG_T

Cuts at:      0    1159    1575 
   Size:       1159     416
 
AleI CACnn'nnGTG

Cuts at:      0     812    1575 
   Size:        812     763
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1277    1309    1575 
   Size:       1277      32     266

  Fragments arranged by size: 

                1277     266      32
 
ApoI r'AATT_y

Cuts at:      0     211    1348    1575 
   Size:        211    1137     227

  Fragments arranged by size: 

                1137     227     211
 
AseI AT'TA_AT

Cuts at:      0     844    1575 
   Size:        844     731
 
BanI G'GyrC_C

Cuts at:      0    1479    1575 
   Size:       1479      96
 
BanII G_rGCy'C

Cuts at:      0     118    1100    1575 
   Size:        118     982     475

  Fragments arranged by size: 

                 982     475     118
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     581    1575 
   Size:        581     994
 
BbvCI CC'TCA_GC

Cuts at:      0      62    1575 
   Size:         62    1513
 
BglII A'GATC_T

Cuts at:      0     963    1575 
   Size:        963     612
 
BmgBI CAC'GTC

Cuts at:      0    1162    1575 
   Size:       1162     413
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     356     890    1077    1336    1575 
   Size:        356     534     187     259     239

  Fragments arranged by size: 

                 534     356     259     239     187
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     460     884    1049    1575 
   Size:        151     309     424     165     526

  Fragments arranged by size: 

                 526     424     309     165     151
 
Bpu10I CC'TnA_GC

Cuts at:      0      62    1575 
   Size:         62    1513
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535    1575 
   Size:        535    1040
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     644    1575 
   Size:        644     931
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     545    1575 
   Size:        148     397    1030

  Fragments arranged by size: 

                1030     397     148
 
BseYI C'CCAG_C

Cuts at:      0      33    1575 
   Size:         33    1542
 
BsiHKAI G_wGCw'C

Cuts at:      0     870    1559    1575 
   Size:        870     689      16

  Fragments arranged by size: 

                 870     689      16
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     870    1100    1559    1575 
   Size:        118     752     230     459      16

  Fragments arranged by size: 

                 752     459     230     118      16
 
BspHI T'CATG_A

Cuts at:      0     732    1575 
   Size:        732     843
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1568    1575 
   Size:       1568       7
 
BsrDI GCAATG_nn'

Cuts at:      0     389    1575 
   Size:        389    1186
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     615    1575 
   Size:        615     960
 
BstYI r'GATC_y

Cuts at:      0     963    1575 
   Size:        963     612
 
BtgI C'CryG_G

Cuts at:      0     165     608    1575 
   Size:        165     443     967

  Fragments arranged by size: 

                 967     443     165
 
BtsI GCAGTG_nn'

Cuts at:      0     578    1575 
   Size:        578     997
 
DraI TTT'AAA

Cuts at:      0    1107    1575 
   Size:       1107     468
 
EaeI y'GGCC_r

Cuts at:      0     804    1385    1549    1575 
   Size:        804     581     164      26

  Fragments arranged by size: 

                 804     581     164      26
 
EarI CTCTTCn'nnn_

Cuts at:      0    1121    1368    1575 
   Size:       1121     247     207

  Fragments arranged by size: 

                1121     247     207
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     460     676     884    1049    1145    1575 
   Size:        151     309     216     208     165      96     430

  Fragments arranged by size: 

                 430     309     216     208     165     151      96
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     474    1296    1397    1575 
   Size:        474     822     101     178

  Fragments arranged by size: 

                 822     474     178     101
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     100     132    1575 
   Size:        100      32    1443

  Fragments arranged by size: 

                1443     100      32
 
HincII GTy'rAC

Cuts at:      0    1176    1575 
   Size:       1176     399
 
HindIII A'AGCT_T

Cuts at:      0    1526    1575 
   Size:       1526      49
 
MfeI C'AATT_G

Cuts at:      0     902    1575 
   Size:        902     673
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     156    1575 
   Size:        156    1419
 
MscI TGG'CCA

Cuts at:      0     806    1575 
   Size:        806     769
 
MslI CAynn'nnrTG

Cuts at:      0     367     812    1575 
   Size:        367     445     763

  Fragments arranged by size: 

                 763     445     367
 
NcoI C'CATG_G

Cuts at:      0     165     608    1575 
   Size:        165     443     967

  Fragments arranged by size: 

                 967     443     165
 
NdeI CA'TA_TG

Cuts at:      0     183    1236    1575 
   Size:        183    1053     339

  Fragments arranged by size: 

                1053     339     183
 
NspI r_CATG'y

Cuts at:      0     858    1575 
   Size:        858     717
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     880    1575 
   Size:        880     695
 
PshAI GACnn'nnGTC

Cuts at:      0    1171    1575 
   Size:       1171     404
 
SalI G'TCGA_C

Cuts at:      0    1174    1575 
   Size:       1174     401
 
SfcI C'TryA_G

Cuts at:      0     645    1575 
   Size:        645     930
 
SmlI C'TyrA_G

Cuts at:      0     514    1575 
   Size:        514    1061
 
SpeI A'CTAG_T

Cuts at:      0     229    1575 
   Size:        229    1346
 
SspI AAT'ATT

Cuts at:      0     356     435     566    1575 
   Size:        356      79     131    1009

  Fragments arranged by size: 

                1009     356     131      79
 
StyI C'CwwG_G

Cuts at:      0     165     608     684    1575 
   Size:        165     443      76     891

  Fragments arranged by size: 

                 891     443     165      76
 
TatI w'GTAC_w

Cuts at:      0    1251    1575 
   Size:       1251     324
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1383    1415    1575 
   Size:       1383      32     160

  Fragments arranged by size: 

                1383     160      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     172    1575 
   Size:        172    1403
 
XmnI GAAnn'nnTTC

Cuts at:      0     728    1076    1575 
   Size:        728     348     499

  Fragments arranged by size: 

                 728     499     348
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AloI     ApoI     AseI
     BanI    BanII     BbsI    BbvCI    BglII    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrDI    BstXI    BstYI     BtgI     BtsI     DraI     EaeI
     EarI  Eco57MI     FalI    Hin4I   HincII  HindIII     MfeI     MmeI
     MscI     MslI     NcoI     NdeI     NspI    PflMI    PshAI     SalI
     SfcI     SmlI     SpeI     SspI     StyI     TatI     TstI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MluI   MspA1I     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SphI     SrfI     StuI     SwaI    TaqII  Tth111I     XbaI
     XhoI     XmaI     ZraI
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