Restriction map for pF1KB4307
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BanI        BbeI        BbsI        BbvCI       
BcgI        BclI        BcnI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       HgaI        HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MamI        MluI        MroI        MspI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII         535  GACGTC               GACGT^C
AcyI          535  GACGTC               G[GA]^CG[CT]C
BalI          749  TGGCCA               TGG^CCA
BamHI         248  GGATCC               G^GATCC
BbrPI         214  CACGTG               CAC^GTG
BbvI          795  GCAGC                GCAGC(8/12)
BglI          219  GCCCAGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          157  CTGGAG               CTGGAG(16/14)
BsaAI         214  CACGTG               [CT]AC^GT[GA]
BsaHI         535  GACGTC               G[GA]^CG[CT]C
BseRI         814  GAGGAG               GAGGAG(10/8)
BsiWI         188  CGTACG               C^GTACG
BsiYI         896  CCAAAGAACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          896  CCAAAGAACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI         533  GAGACG               CGTCTC(1/5)
BsmFI         177  GGGAC                GGGAC(10/14)
BspMI         640  GCAGGT               ACCTGC(4/8)
CfoI          350  GCGC                 GCG^C
Cfr13I        660  GGCCC                G^G[ACGT]CC
EaeI          749  TGGCCA               [CT]^GGCC[GA]
EcoRI         891  GAATTC               G^AATTC
EcoT14I       104  CCAAGG               C^C[AT][AT]GG
FauI          224  GCGGG                CCCGC(4/6)
HhaI          350  GCGC                 GCG^C
Hin1I         535  GACGTC               G[GA]^CG[CT]C
HinP1I        350  GCGC                 G^CGC
HincII        862  GTTGAC               GT[CT]^[GA]AC
HindII        862  GTTGAC               GT[CT]^[GA]AC
MaeIII        337  GTCAC                ^GT[ACGT]AC
MfeI          632  CAATTG               C^AATTG
MluNI         749  TGGCCA               TGG^CCA
MscI          749  TGGCCA               TGG^CCA
MspA1I        222  CAGCGG               C[AC]G^C[GT]G
MunI          632  CAATTG               C^AATTG
PmaCI         214  CACGTG               CAC^GTG
PmlI          214  CACGTG               CAC^GTG
PstI          851  CTGCAG               CTGCA^G
Sau96I        660  GGCCC                G^G[ACGT]CC
SfcI          851  CTGCAG               C^T[GA][CT]AG
StyI          104  CCAAGG               C^C[AT][AT]GG
TseI          795  GCAGC                G^C[AT]GC
Tsp45I        337  GTCAC                ^GT[GC]AC
XcmI          308  CCATGAGGACGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI          189  GTAC                 GT^AC
              553  GTAC                
AspHI         388  GAGCTC               G[AT]GC[AT]^C
              682  GAGCTC              
BanII         388  GAGCTC               G[GA]GC[CT]^C
              682  GAGCTC              
BciVI         396  GGATAC               GTATCC(6/5)
              697  GGATAC              
BfaI           89  CTAG                 C^TAG
              406  CTAG                
BsaJI          30  CCCTGG               C^C[ACGT][ACGT]GG
              104  CCAAGG              
BsiHKAI       388  GAGCTC               G[AT]GC[AT]^C
              682  GAGCTC              
BsrI          174  ACTGG                ACTGG(1/-1)
               74  CCAGT               
BstF5I        753  CATCC                GGATG(2/0)
              931  CATCC               
BstYI         248  GGATCC               [GA]^GATC[CT]
              377  GGATCT              
Cac8I         109  GCTTGC               GC[ACGT]^[ACGT]GC
              224  GCGGGC              
Fnu4HI        210  GCGGC                GC^[ACGT]GC
              795  GCAGC               
FokI          753  CATCC                GGATG(9/13)
              931  CATCC               
ItaI          210  GCGGC                GC^[ACGT]GC
              795  GCAGC               
MaeI           89  CTAG                 C^TAG
              406  CTAG                
MaeII         215  ACGT                 A^CGT
              536  ACGT                
MflI          248  GGATCC               [GA]^GATC[CT]
              377  GGATCT              
MseI           38  TTAA                 T^TAA
              883  TTAA                
MslI          234  CATGGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              732  CATCACGATG          
MwoI          210  GCGGCACGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              219  GCCCAGCGGGC         
RsaI          189  GTAC                 GT^AC
              553  GTAC                
SacI          388  GAGCTC               GAGCT^C
              682  GAGCTC              
TaqI           10  TCGA                 T^CGA
              759  TCGA                
Tru9I          38  TTAA                 T^TAA
              883  TTAA                
TthHB8I        10  TCGA                 T^CGA
              759  TCGA                
XhoII         248  GGATCC               [GA]^GATC[CT]
              377  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
BmyI        BsmAI       Bsp1286I    BssKI       BstNI       
DdeI        DpnII       EcoRII      HaeIII      HinfI       
HphI        MboI        MboII       MnlI        MvaI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
ScrFI       SfaNI       TfiI        Tsp509I     TspRI       
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