Restriction map for pF1KB4307
 (Circular) (Six-base) MAPSORT of: pF1KB4307.seq  Check: 9659  from: 1  to: 4115



pF1KB4307  4115 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AatII G_ACGT'C

Cuts at:    647     647 
   Size:       4115
 
AccI GT'mk_AC

Cuts at:   1153    1153 
   Size:       4115
 
Acc65I G'GTAC_C

Cuts at:   1131    1131 
   Size:       4115
 
AclI AA'CG_TT

Cuts at:   3955    3955 
   Size:       4115
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1940    2372    2725    3387    1940 
   Size:        432     353     662    2668

  Fragments arranged by size: 

                2668     662     432     353
 
AflIII A'CryG_T

Cuts at:   1467    3267    3548    1467 
   Size:       1800     281    2034

  Fragments arranged by size: 

                2034    1800     281
 
AlwNI CAG_nnn'CTG

Cuts at:   1420    2858    1420 
   Size:       1438    2677
 
ApaLI G'TGCA_C

Cuts at:   2953    2953 
   Size:       4115
 
ApoI r'AATT_y

Cuts at:    149     520     999    1119    3468    3724     149 
   Size:        371     479     120    2349     256     540

  Fragments arranged by size: 

                2349     540     479     371     256     120
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4115
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4115
 
AvaI C'yCGr_G

Cuts at:   1135    1135 
   Size:       4115
 
BamHI G'GATC_C

Cuts at:    356    1140     356 
   Size:        784    3331
 
BanI G'GyrC_C

Cuts at:   1131    1545    1793    1828    1131 
   Size:        414     248      35    3418

  Fragments arranged by size: 

                3418     414     248      35
 
BanII G_rGCy'C

Cuts at:    500     794    1129    2159     500 
   Size:        294     335    1030    2456

  Fragments arranged by size: 

                2456    1030     335     294
 
BbeI G_GCGC'C

Cuts at:   1549    1797    1549 
   Size:        248    3867
 
BciVI GTATCCnnnnn_n'

Cuts at:    498     799    2013    3069    4115     498 
   Size:        301    1214    1056    1046     498

  Fragments arranged by size: 

                1214    1056    1046     498     301
 
BclI T'GATC_A

Cuts at:   1635    1635 
   Size:       4115
 
BglI GCCn_nnn'nGGC

Cuts at:    333     333 
   Size:       4115
 
BglII A'GATC_T

Cuts at:   1630    3667    1630 
   Size:       2037    2078
 
BlpI GC'TnA_GC

Cuts at:   1227    1227 
   Size:       4115
 
Bme1580I G_kGCm'C

Cuts at:    329    1740    1833    2957     329 
   Size:       1411      93    1124    1487

  Fragments arranged by size: 

                1487    1411    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    292    1327    1408    1743    3887     292 
   Size:       1035      81     335    2144     520

  Fragments arranged by size: 

                2144    1035     520     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2482    2482 
   Size:       4115
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    286     286 
   Size:       4115
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1291    2170    2884    3182    1291 
   Size:        879     714     298    2224

  Fragments arranged by size: 

                2224     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3819    3819 
   Size:       4115
 
BsaAI yAC'GTr

Cuts at:    324    1468     324 
   Size:       1144    2971
 
BsaBI GATnn'nnATC

Cuts at:      6    1629    3666       6 
   Size:       1623    2037     455

  Fragments arranged by size: 

                2037    1623     455
 
BsaHI Gr'CG_yC

Cuts at:    644    1546    1794    2496    3992     644 
   Size:        902     248     702    1496     767

  Fragments arranged by size: 

                1496     902     767     702     248
 
BsaWI w'CCGG_w

Cuts at:   1304    1825    2503    2914    3061    3335    1304 
   Size:        521     678     411     147     274    2084

  Fragments arranged by size: 

                2084     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    937     937 
   Size:       4115
 
BseYI C'CCAG_C

Cuts at:    328     942    1540    2298    2963    3371     328 
   Size:        614     598     758     665     408    1072

  Fragments arranged by size: 

                1072     758     665     614     598     408
 
BsiEI CG_ry'CG

Cuts at:    104    1657    1703    2933     104 
   Size:       1553      46    1230    1286

  Fragments arranged by size: 

                1553    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    500     794    1129    1907    2097    2957     500 
   Size:        294     335     778     190     860    1658

  Fragments arranged by size: 

                1658     860     778     335     294     190
 
BsiWI C'GTAC_G

Cuts at:    296     296 
   Size:       4115
 
BsmBI CGTCTCn'nnnn_

Cuts at:    635    1100     635 
   Size:        465    3650
 
Bsp1286I G_dGCh'C

Cuts at:    329     500     794    1129    1740    1833    1907    2097    2159 
   Size:        171     294     335     611      93      74     190      62

Cuts at:   2159    2957     329 
   Size:        798    1487

  Fragments arranged by size: 

                1487     798     611     335     294     190     171      93
                  74      62
 
BspHI T'CATG_A

Cuts at:   2547    2547 
   Size:       4115
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    739    1165    1379    1681    2062     739 
   Size:        426     214     302     381    2792

  Fragments arranged by size: 

                2792     426     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2407    2461    3945    4110      50 
   Size:       2357      54    1484     165      55

  Fragments arranged by size: 

                2357    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2027    2027 
   Size:       4115
 
BsrFI r'CCGG_y

Cuts at:   2113    2294    2113 
   Size:        181    3934
 
BssSI C'ACGA_G

Cuts at:   2386    3094    2386 
   Size:        708    3407
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1492    1492 
   Size:       4115
 
BstBI TT'CG_AA

Cuts at:   2476    2476 
   Size:       4115
 
BstYI r'GATC_y

Cuts at:      1     356     485    1140    1607    1630    1965    2211    2517 
   Size:        355     129     655     467      23     335     246     306

Cuts at:   2517    2529    2615    3667       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1052     655     467     449     355     335     306     246
                 129      86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    914    2065    2246    2409     914 
   Size:       1151     181     163    2620

  Fragments arranged by size: 

                2620    1151     181     163
 
BtsI GCAGTG_nn'

Cuts at:    353    1358     353 
   Size:       1005    3110
 
DraI TTT'AAA

Cuts at:   1114    1114 
   Size:       4115
 
DrdI GACnn_nn'nnGTC

Cuts at:   1821    3165    1821 
   Size:       1344    2771
 
EaeI y'GGCC_r

Cuts at:    857    1700    1874    2265    2292    3637     857 
   Size:        843     174     391      27    1345    1335

  Fragments arranged by size: 

                1345    1335     843     391     174      27
 
EagI C'GGCC_G

Cuts at:   1700    1700 
   Size:       4115
 
EarI CTCTTCn'nnn_

Cuts at:   2138    2348    2138 
   Size:        210    3905
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2323    3065    3211    3925    2323 
   Size:        742     146     714    2513

  Fragments arranged by size: 

                2513     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    498     792    1127     498 
   Size:        294     335    3486

  Fragments arranged by size: 

                3486     335     294
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    286    1940    2372    2725    3387     286 
   Size:       1654     432     353     662    1014

  Fragments arranged by size: 

                1654    1014     662     432     353
 
EcoO109I rG'GnC_Cy

Cuts at:   1254    1254 
   Size:       4115
 
EcoRI G'AATT_C

Cuts at:    999    1119     999 
   Size:        120    3995
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2134    2134 
   Size:       4115
 
FspI TGC'GCA

Cuts at:   1896    1896 
   Size:       4115
 
HaeII r_GCGC'y

Cuts at:   1549    1797    3027    3798    1549 
   Size:        248    1230     771    1866

  Fragments arranged by size: 

                1866    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    193     225     913     940     945     964     972     996    1066 
   Size:         32     688      27       5      19       8      24      70

Cuts at:   1066    1098    1957    1987    1989    2019     193 
   Size:         32     859      30       2      30    2289

  Fragments arranged by size: 

                2289     859     688      70      32      32      30      30
                  27      24      19       8       5       2
 
HincII GTy'rAC

Cuts at:    972    1154     972 
   Size:        182    3933
 
KasI G'GCGC_C

Cuts at:   1545    1793    1545 
   Size:        248    3867
 
KpnI G_GTAC'C

Cuts at:   1135    1135 
   Size:       4115
 
MfeI C'AATT_G

Cuts at:    740     740 
   Size:       4115
 
MluI A'CGCG_T

Cuts at:   3548    3548 
   Size:       4115
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2874    3058    2874 
   Size:        184    3931
 
MscI TGG'CCA

Cuts at:    859    1876     859 
   Size:       1017    3098
 
MslI CAynn'nnrTG

Cuts at:    346     844    2104    2231     346 
   Size:        498    1260     127    2230

  Fragments arranged by size: 

                2230    1260     498     127
 
MspA1I CmG'CkG

Cuts at:    332    1226    1540    1900    2684    2929     332 
   Size:        894     314     360     784     245    1518

  Fragments arranged by size: 

                1518     894     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   2296    2296 
   Size:       4115
 
NarI GG'CG_CC

Cuts at:   1546    1794    1546 
   Size:        248    3867
 
NgoMIV G'CCGG_C

Cuts at:   2294    2294 
   Size:       4115
 
NspI r_CATG'y

Cuts at:   1168    1668    3271    1168 
   Size:        500    1603    2012

  Fragments arranged by size: 

                2012    1603     500
 
PciI A'CATG_T

Cuts at:   3267    3267 
   Size:       4115
 
PmeI GTTT'AAAC

Cuts at:   1114    1114 
   Size:       4115
 
PmlI CAC'GTG

Cuts at:    324     324 
   Size:       4115
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2561    2593    2561 
   Size:         32    4083
 
PstI C_TGCA'G

Cuts at:    963    1162    1847     963 
   Size:        199     685    3231

  Fragments arranged by size: 

                3231     685     199
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4115
 
PvuII CAG'CTG

Cuts at:   1540    1900    1540 
   Size:        360    3755
 
RsrII CG'GwC_CG

Cuts at:   2310    2310 
   Size:       4115
 
SacI G_AGCT'C

Cuts at:    500     794    1129     500 
   Size:        294     335    3486

  Fragments arranged by size: 

                3486     335     294
 
SalI G'TCGA_C

Cuts at:   1152    1152 
   Size:       4115
 
SapI GCTCTTCn'nnn_

Cuts at:   2138    2348    2138 
   Size:        210    3905
 
SbfI CC_TGCA'GG

Cuts at:   1162    1162 
   Size:       4115
 
ScaI AGT'ACT

Cuts at:   3632    3632 
   Size:       4115
 
SfcI C'TryA_G

Cuts at:     33     959    1158    1843    2811    3002      33 
   Size:        926     199     685     968     191    1146

  Fragments arranged by size: 

                1146     968     926     685     199     191
 
SfoI GGC'GCC

Cuts at:   1547    1795    1547 
   Size:        248    3867
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4115
 
SmaI CCC'GGG

Cuts at:   1137    1137 
   Size:       4115
 
SmlI C'TyrA_G

Cuts at:   1270    2185    2899    3161    1270 
   Size:        915     714     262    2224

  Fragments arranged by size: 

                2224     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4115
 
SphI G_CATG'C

Cuts at:   1168    1668    1168 
   Size:        500    3615
 
StyI C'CwwG_G

Cuts at:    212    1249    1603     212 
   Size:       1037     354    2724

  Fragments arranged by size: 

                2724    1037     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    667    1698    1831    2290    2499     667 
   Size:       1031     133     459     209    2283

  Fragments arranged by size: 

                2283    1031     459     209     133
 
TatI w'GTAC_w

Cuts at:    660    3630     660 
   Size:       2970    1145
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    797     829    1061    1093    3632    3664     797 
   Size:         32     232      32    2539      32    1248

  Fragments arranged by size: 

                2539    1248     232      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1912    1912 
   Size:       4115
 
XbaI T'CTAG_A

Cuts at:   1146    1146 
   Size:       4115
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    423    1610     423 
   Size:       1187    2928
 
XmaI C'CCGG_G

Cuts at:   1135    1135 
   Size:       4115
 
ZraI GAC'GTC

Cuts at:    645     645 
   Size:       4115
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PciI     PmeI     PmlI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AgeI     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
    BmgBI     BmtI   Bpu10I    BsaXI     BsgI     BsmI    BspEI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI
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