Restriction map for pF1KB4173
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AgeI        AocI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BbrPI       BbsI        BbvCI       
BcgI        BclI        BfrI        BglII       BlnI        
BpuAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
MamI        MfeI        MluI        MroI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
RcaI        RsrII       SapI        ScaI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        TfiI        Tsp509I     
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AhdI          907  GACGGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        664  GTGCAC               G^TGCAC
ApaLI         664  GTGCAC               G^TGCAC
AspEI         907  GACGGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI        1075  GGATAC               GTATCC(6/5)
BfaI         2204  CTAG                 C^TAG
BlpI          804  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      804  GCTCAGC              GC^T[ACGT]AGC
BsrBI        1797  CCGCTC               CCGCTC(-3/-3)
BsrDI         944  GCAATG               GCAATG(2/0)
BssSI          52  CTCGTG               CACGAG(-5/-1)
CelII         804  GCTCAGC              GC^T[ACGT]AGC
Eam1105I      907  GACGGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         2179  CTCTTC               CTCTTC(1/4)
HgaI         2415  GACGC                GACGC(5/10)
HincII        993  GTCGAC               GT[CT]^[GA]AC
HindII        993  GTCGAC               GT[CT]^[GA]AC
Ksp632I      2179  CTCTTC               CTCTTC(1/4)
KspI          315  CCGCGG               CCGC^GG
MaeI         2204  CTAG                 C^TAG
MseI         1901  TTAA                 T^TAA
NspI         1541  GCATGT               [GA]CATG^[CT]
PflMI        1155  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1375  GACGAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         2651  CGATCG               CGAT^CG
SacII         315  CCGCGG               CCGC^GG
SalI          993  GTCGAC               G^TCGAC
SbfI         2475  CCTGCAGG             CCTGCA^GG
SexAI        1523  ACCAGGT              A^CC[AT]GGT
Sse8387I     2475  CCTGCAGG             CCTGCA^GG
StuI          774  AGGCCT               AGG^CCT
Tru9I        1901  TTAA                 T^TAA
Van91I       1155  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AccI          993  GTCGAC               GT^[AC][GT]AC
             1015  GTCTAC              
AflIII       1847  ACGTGT               A^C[GA][CT]GT
             2670  ACGTGT              
BamHI        1004  GGATCC               G^GATCC
             1326  GGATCC              
BbeI          708  GGCGCC               GGCGC^C
             2733  GGCGCC              
BsaAI        1627  TACGTG               [CT]AC^GT[GA]
             2669  TACGTG              
DrdI         2791  GACACCAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2806  GACATCTGTGTC        
EcoNI        1745  CCTCCCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2538  CCTGACCAAGG         
KasI          708  GGCGCC               G^GCGCC
             2733  GGCGCC              
NarI          708  GGCGCC               GG^CGCC
             2733  GGCGCC              
SacI          423  GAGCTC               GAGCT^C
             1854  GAGCTC              
SfaNI         199  GATGC                GCATC(5/9)
              801  GATGC               
SfiI         2070  GGCCCAGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2271  GGCCGGGAAGGCC       
SfoI          708  GGCGCC               GGC^GCC
             2733  GGCGCC              
SmaI          194  CCCGGG               CCC^GGG
             1444  CCCGGG              
XmaCI         194  CCCGGG               C^CCGGG
             1444  CCCGGG              
XmaI          194  CCCGGG               C^CCGGG
             1444  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EagI        EclXI       
Eco52I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XcmI        XhoII       
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