Restriction map for pF1KB4122
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AlwNI       
Aor51HI     AscI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BsaWI       BseAI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       BstBI       BstEII      BstPI       
BstXI       CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
EcoNI       EcoO65I     EcoRV       FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        MamI        MfeI        
MluI        MroI        MslI        MunI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
Psp1406I    PvuI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccII          28  CGCG                 CG^CG
AfaI         1304  GTAC                 GT^AC
AocI          616  CCTAAGG              CC^T[ACGT]AGG
ApaI           79  GGGCCC               GGGCC^C
AseI         1399  ATTAAT               AT^TAAT
AsnI         1399  ATTAAT               AT^TAAT
AvaI          527  CTCGGG               C^[CT]CG[GA]G
BanI          519  GGCACC               G^G[CT][GA]CC
BanII          79  GGGCCC               G[GA]GC[CT]^C
BciVI        1109  GTATCC               GTATCC(6/5)
BcnI           33  CCGGG                CC^[GC]GG
BpmI         1203  CTCCAG               CTGGAG(16/14)
BsaAI         632  TACGTG               [CT]AC^GT[GA]
BsaI           23  GAGACC               GGTCTC(1/5)
BsiEI          30  CGGCCG               CG[GA][CT]^CG
BsmI          755  GAATGC               GAATGC(1/-1)
BsoBI         527  CTCGGG               C^[CT]CG[GA]G
Bst1107I     1658  GTATAC               GTA^TAC
BstAPI       1780  GCATATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          28  CGCG                 CG^CG
BstYI         421  GGATCT               [GA]^GATC[CT]
BstZ17I      1658  GTATAC               GTA^TAC
Bsu36I        616  CCTAAGG              CC^T[ACGT]AGG
CfoI          201  GCGC                 GCG^C
EagI           30  CGGCCG               C^GGCCG
EarI         1000  GAAGAG               CTCTTC(1/4)
EclXI          30  CGGCCG               C^GGCCG
Eco52I         30  CGGCCG               C^GGCCG
Eco81I        616  CCTAAGG              CC^T[ACGT]AGG
EcoRI        1156  GAATTC               G^AATTC
EcoT22I      1778  ATGCAT               ATGCA^T
FauI         1495  GCGGG                CCCGC(4/6)
HapII          33  CCGG                 C^CGG
HhaI          201  GCGC                 GCG^C
HinP1I        201  GCGC                 G^CGC
HincII       1127  GTCAAC               GT[CT]^[GA]AC
HindII       1127  GTCAAC               GT[CT]^[GA]AC
HpaII          33  CCGG                 C^CGG
HphI         1117  GGTGA                GGTGA(8/7)
Ksp632I      1000  GAAGAG               CTCTTC(1/4)
KspI           27  CCGCGG               CCGC^GG
MflI          421  GGATCT               [GA]^GATC[CT]
MspI           33  CCGG                 C^CGG
MvnI           28  CGCG                 CG^CG
NciI           33  CCGGG                CC^[GC]GG
NdeI         1775  CATATG               CA^TATG
NsiI         1778  ATGCAT               ATGCA^T
NspI          577  GCATGT               [GA]CATG^[CT]
PleI         1125  GAGTC                GAGTC(4/5)
PshAI        1120  GACAAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1399  ATTAAT               AT^TAAT
PstI         1793  CTGCAG               CTGCA^G
PvuII        1796  CAGCTG               CAG^CTG
RsaI         1304  GTAC                 GT^AC
SacII          27  CCGCGG               CCGC^GG
SspI          566  AATATT               AAT^ATT
TfiI          138  GAATC                G^A[AT]TC
XcmI         1031  CCATTGTTTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         421  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          591  GTCTAC               GT^[AC][GT]AC
             1658  GTATAC              
Alw44I         50  GTGCAC               G^TGCAC
              908  GTGCAC              
AlwI          421  GGATC                GGATC(4/5)
              798  GGATC               
ApaLI          50  GTGCAC               G^TGCAC
              908  GTGCAC              
AvaII          36  GGACC                G^G[AT]CC
              622  GGACC               
BalI          373  TGGCCA               TGG^CCA
              844  TGGCCA              
BbsI          677  GAAGAC               GAAGAC(2/6)
             1426  GAAGAC              
BpuAI         677  GAAGAC               GAAGAC(2/6)
             1426  GAAGAC              
BsgI         1799  CTGCAC               GTGCAG(16/14)
              547  GTGCAG              
BsmAI          23  GAGAC                GTCTC(1/5)
             1526  GAGAC               
BspHI         960  TCATGA               T^CATGA
             1007  TCATGA              
DraII          78  AGGGCCC              [GA]G^G[ACGT]CC[CT]
               79  GGGCCCT             
EcoO109I       78  AGGGCCC              [GA]G^G[ACGT]CC[CT]
               79  GGGCCCT             
EcoT14I      1103  CCATGG               C^C[AT][AT]GG
             1113  CCATGG              
HinfI         138  GAATC                G^A[ACGT]TC
             1125  GAGTC               
MaeII         633  ACGT                 A^CGT
              965  ACGT                
MluNI         373  TGGCCA               TGG^CCA
              844  TGGCCA              
MscI          373  TGGCCA               TGG^CCA
              844  TGGCCA              
MspA1I         27  CCGCGG               C[AC]G^C[GT]G
             1796  CAGCTG              
NcoI         1103  CCATGG               C^CATGG
             1113  CCATGG              
RcaI          960  TCATGA               T^CATGA
             1007  TCATGA              
SfcI         1190  CTACAG               C^T[GA][CT]AG
             1793  CTGCAG              
StyI         1103  CCATGG               C^C[AT][AT]GG
             1113  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        AspHI       
BbvI        BfaI        BmyI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EcoRII      Fnu4HI      FokI        HaeIII      ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
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