Restriction map for pF1KB4115
 (Linear) (Six-base) MAPSORT of: KIBB4115.seq  Check: 3262  from: 1  to: 2814



KIBB4115.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AccI GT'mk_AC

Cuts at:      0    1155    2814 
   Size:       1155    1659
 
AclI AA'CG_TT

Cuts at:      0     254    2814 
   Size:        254    2560
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383    1102    1475    1553    2357    2814 
   Size:        383     719     373      78     804     457

  Fragments arranged by size: 

                 804     719     457     383     373      78
 
AflIII A'CryG_T

Cuts at:      0    1866    2814 
   Size:       1866     948
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1043    2814 
   Size:       1043    1771
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      74     106    2814 
   Size:         74      32    2708

  Fragments arranged by size: 

                2708      74      32
 
ApoI r'AATT_y

Cuts at:      0    1480    2211    2814 
   Size:       1480     731     603

  Fragments arranged by size: 

                1480     731     603
 
AvrII C'CTAG_G

Cuts at:      0    1971    2814 
   Size:       1971     843
 
BanI G'GyrC_C

Cuts at:      0    2320    2814 
   Size:       2320     494
 
BanII G_rGCy'C

Cuts at:      0    2002    2352    2693    2814 
   Size:       2002     350     341     121

  Fragments arranged by size: 

                2002     350     341     121
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     539    2814 
   Size:        539    2275
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     784     818    2814 
   Size:        784      34    1996

  Fragments arranged by size: 

                1996     784      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2521    2814 
   Size:       2521     293
 
BclI T'GATC_A

Cuts at:      0     609    2814 
   Size:        609    2205
 
BglI GCCn_nnn'nGGC

Cuts at:      0     118    2814 
   Size:        118    2696
 
BglII A'GATC_T

Cuts at:      0     699    1887    2814 
   Size:        699    1188     927

  Fragments arranged by size: 

                1188     927     699
 
Bme1580I G_kGCm'C

Cuts at:      0    2325    2814 
   Size:       2325     489
 
BmgBI CAC'GTC

Cuts at:      0    2497    2814 
   Size:       2497     317
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1642    2408    2814 
   Size:       1642     766     406

  Fragments arranged by size: 

                1642     766     406
 
BmtI G_CTAG'C

Cuts at:      0    1543    2814 
   Size:       1543    1271
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     732    2814 
   Size:        732    2082
 
BsaBI GATnn'nnATC

Cuts at:      0     338    1595    2567    2814 
   Size:        338    1257     972     247

  Fragments arranged by size: 

                1257     972     338     247
 
BsaHI Gr'CG_yC

Cuts at:      0    1470    2814 
   Size:       1470    1344
 
BsaWI w'CCGG_w

Cuts at:      0    1841    2814 
   Size:       1841     973
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      74     104    2814 
   Size:         74      30    2710

  Fragments arranged by size: 

                2710      74      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1100    1495    1979    2814 
   Size:       1100     395     484     835

  Fragments arranged by size: 

                1100     835     484     395
 
BseYI C'CCAG_C

Cuts at:      0      67    2176    2814 
   Size:         67    2109     638

  Fragments arranged by size: 

                2109     638      67
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     722    1399    2219    2814 
   Size:        722     677     820     595

  Fragments arranged by size: 

                 820     722     677     595
 
BsmI GAATG_Cn'

Cuts at:      0     352    1908    2814 
   Size:        352    1556     906

  Fragments arranged by size: 

                1556     906     352
 
Bsp1286I G_dGCh'C

Cuts at:      0    2002    2325    2352    2693    2814 
   Size:       2002     323      27     341     121

  Fragments arranged by size: 

                2002     341     323     121      27
 
BspEI T'CCGG_A

Cuts at:      0    1841    2814 
   Size:       1841     973
 
BsrBI CCG'CTC

Cuts at:      0    1774    1986    2814 
   Size:       1774     212     828

  Fragments arranged by size: 

                1774     828     212
 
BsrDI GCAATG_nn'

Cuts at:      0    1901    2099    2814 
   Size:       1901     198     715

  Fragments arranged by size: 

                1901     715     198
 
BsrFI r'CCGG_y

Cuts at:      0    2606    2814 
   Size:       2606     208
 
BssSI C'ACGA_G

Cuts at:      0    1727    2814 
   Size:       1727    1087
 
BstEII G'GTnAC_C

Cuts at:      0    1831    2814 
   Size:       1831     983
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2524    2814 
   Size:       2524     290
 
BstYI r'GATC_y

Cuts at:      0     187     699    1022    1887    2814 
   Size:        187     512     323     865     927

  Fragments arranged by size: 

                 927     865     512     323     187
 
BtgI C'CryG_G

Cuts at:      0    1584    1677    2517    2814 
   Size:       1584      93     840     297

  Fragments arranged by size: 

                1584     840     297      93
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     347    2814 
   Size:        347    2467
 
BtsI GCAGTG_nn'

Cuts at:      0     992    2814 
   Size:        992    1822
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1650    1685    2298    2333    2814 
   Size:       1650      35     613      35     481

  Fragments arranged by size: 

                1650     613     481      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1648    2263    2337    2814 
   Size:       1648     615      74     477

  Fragments arranged by size: 

                1648     615     477      74
 
EaeI y'GGCC_r

Cuts at:      0    1073    1343    1658    1758    2565    2814 
   Size:       1073     270     315     100     807     249

  Fragments arranged by size: 

                1073     807     315     270     249     100
 
EarI CTCTTCn'nnn_

Cuts at:      0     135     388     449     521     602     659    1494    1529 
   Size:        135     253      61      72      81      57     835      35

Cuts at:   1529    1764    2814 
   Size:        235    1050

  Fragments arranged by size: 

                1050     835     253     235     135      81      72      61
                  57      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383    1102    1475    1553    2357    2814 
   Size:        383     719     373      78     804     457

  Fragments arranged by size: 

                 804     719     457     383     373      78
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     749    1120    1555    2063    2814 
   Size:        749     371     435     508     751

  Fragments arranged by size: 

                 751     749     508     435     371
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1113    2814 
   Size:       1113    1701
 
EcoRI G'AATT_C

Cuts at:      0    2211    2814 
   Size:       2211     603
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1220    2145    2814 
   Size:       1220     925     669

  Fragments arranged by size: 

                1220     925     669
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     612     644     981    1013    2460    2492    2814 
   Size:        612      32     337      32    1447      32     322

  Fragments arranged by size: 

                1447     612     337     322      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1156    2361    2814 
   Size:       1156    1205     453

  Fragments arranged by size: 

                1205    1156     453
 
HindIII A'AGCT_T

Cuts at:      0     473    2663    2814 
   Size:        473    2190     151

  Fragments arranged by size: 

                2190     473     151
 
MfeI C'AATT_G

Cuts at:      0     566     980    2814 
   Size:        566     414    1834

  Fragments arranged by size: 

                1834     566     414
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     941    2245    2620    2814 
   Size:        941    1304     375     194

  Fragments arranged by size: 

                1304     941     375     194
 
MscI TGG'CCA

Cuts at:      0    1345    1660    2567    2814 
   Size:       1345     315     907     247

  Fragments arranged by size: 

                1345     907     315     247
 
MslI CAynn'nnrTG

Cuts at:      0    2504    2522    2561    2814 
   Size:       2504      18      39     253

  Fragments arranged by size: 

                2504     253      39      18
 
MspA1I CmG'CkG

Cuts at:      0    2758    2814 
   Size:       2758      56
 
NaeI GCC'GGC

Cuts at:      0    2608    2814 
   Size:       2608     206
 
NcoI C'CATG_G

Cuts at:      0    1584    2517    2814 
   Size:       1584     933     297

  Fragments arranged by size: 

                1584     933     297
 
NgoMIV G'CCGG_C

Cuts at:      0    2606    2814 
   Size:       2606     208
 
NheI G'CTAG_C

Cuts at:      0    1539    2814 
   Size:       1539    1275
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     508     973    2296    2814 
   Size:        508     465    1323     518

  Fragments arranged by size: 

                1323     518     508     465
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      74     106    2814 
   Size:         74      32    2708

  Fragments arranged by size: 

                2708      74      32
 
PstI C_TGCA'G

Cuts at:      0     915    2814 
   Size:        915    1899
 
PvuII CAG'CTG

Cuts at:      0    2758    2814 
   Size:       2758      56
 
SalI G'TCGA_C

Cuts at:      0    1154    2814 
   Size:       1154    1660
 
SapI GCTCTTCn'nnn_

Cuts at:      0     135    1529    1764    2814 
   Size:        135    1394     235    1050

  Fragments arranged by size: 

                1394    1050     235     135
 
SexAI A'CCwGG_T

Cuts at:      0     966    1835    2814 
   Size:        966     869     979

  Fragments arranged by size: 

                 979     966     869
 
SfcI C'TryA_G

Cuts at:      0     911    1977    2814 
   Size:        911    1066     837

  Fragments arranged by size: 

                1066     911     837
 
SpeI A'CTAG_T

Cuts at:      0    2194    2814 
   Size:       2194     620
 
StuI AGG'CCT

Cuts at:      0    1970    2050    2814 
   Size:       1970      80     764

  Fragments arranged by size: 

                1970     764      80
 
StyI C'CwwG_G

Cuts at:      0     344    1109    1584    1811    1971    2279    2352    2517 
   Size:        344     765     475     227     160     308      73     165

Cuts at:   2517    2814 
   Size:        297

  Fragments arranged by size: 

                 765     475     344     308     297     227     165     160
                  73
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2177    2814 
   Size:       2177     637
 
TatI w'GTAC_w

Cuts at:      0    2117    2814 
   Size:       2117     697
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      32    2814 
   Size:         32    2782
 
Tth111I GACn'n_nGTC

Cuts at:      0    1837    2814 
   Size:       1837     977
 
XbaI T'CTAG_A

Cuts at:      0     540    2814 
   Size:        540    2274
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     112    2070    2814 
   Size:        112    1958     744

  Fragments arranged by size: 

                1958     744     112
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AlfI     AloI     ApoI    AvrII
     BanI    BanII     BbsI     BcgI    BciVI     BclI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI     BsmI Bsp1286I    BspEI    BsrBI
    BsrDI    BsrFI    BssSI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI    CspCI     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    Hin4I   HincII  HindIII     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI    PflMI     PpiI
     PstI    PvuII     SalI     SapI    SexAI     SfcI     SpeI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI     BaeI
    BamHI     BbeI    BbvCI    BfrBI     BlpI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI    BspMI
    BsrGI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI     DraI
   DraIII     EagI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MluI     NarI     NdeI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII    SanDI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     SspI
     SwaI     XhoI     XmaI     XmnI     ZraI
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