Restriction map for pF1KB4107
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
AlwNI       Aor51HI     AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BlnI        BsaAI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Cfr10I      ClaI        CpoI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AflIII        698  ACATGT               A^C[GA][CT]GT
Alw44I        865  GTGCAC               G^TGCAC
ApaI         1354  GGGCCC               GGGCC^C
ApaLI         865  GTGCAC               G^TGCAC
Asp700I       268  GAACGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          530  CTCGGG               C^[CT]CG[GA]G
BamHI         144  GGATCC               G^GATCC
BbsI          694  GAAGAC               GAAGAC(2/6)
BbvCI         509  CCTCAGC              CCTCAGC(-5/-2)
BcnI           74  CCCGG                CC^[GC]GG
BglI         1266  GCCTCCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          391  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      391  GCTCAGC              GC^T[ACGT]AGC
BpuAI         694  GAAGAC               GAAGAC(2/6)
BsaI         1326  GAGACC               GGTCTC(1/5)
BsoBI         530  CTCGGG               C^[CT]CG[GA]G
BspLU11I      698  ACATGT               A^CATGT
BssSI        1451  CTCGTG               CACGAG(-5/-1)
BstXI        1134  CCATTCTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I        1170  GCAGGC               GC[ACGT]^[ACGT]GC
CelII         391  GCTCAGC              GC^T[ACGT]AGC
DraI          132  TTTAAA               TTT^AAA
EaeI          645  TGGCCG               [CT]^GGCC[GA]
EcoNI        1408  CCTCCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII          75  CCGG                 C^CGG
HpaII          75  CCGG                 C^CGG
HphI          880  GGTGA                GGTGA(8/7)
MspI           75  CCGG                 C^CGG
NciI           74  CCCGG                CC^[GC]GG
PstI          125  CTGCAG               CTGCA^G
SacI         1310  GAGCTC               GAGCT^C
SfiI         1265  GGCCTCCTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          626  CTCAAG               C^T[CT][GA]AG
SspI          368  AATATT               AAT^ATT
StuI         1284  AGGCCT               AGG^CCT
XmnI          268  GAACGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AocI          775  CCTGAGG              CC^T[ACGT]AGG
             1027  CCTGAGG             
AspHI         865  GTGCAC               G[AT]GC[AT]^C
             1310  GAGCTC              
BanI          478  GGTGCC               G^G[CT][GA]CC
             1533  GGTGCC              
BglII          10  AGATCT               A^GATCT
              669  AGATCT              
BsaBI          11  GATCTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              766  GATGGCTATC          
BsiHKAI       865  GTGCAC               G[AT]GC[AT]^C
             1310  GAGCTC              
BsmI          387  GAATGC               GAATGC(1/-1)
             1337  GAATGC              
BspHI         732  TCATGA               T^CATGA
             1898  TCATGA              
BsrI          199  CCAGT                ACTGG(1/-1)
              755  CCAGT               
Bsu36I        775  CCTGAGG              CC^T[ACGT]AGG
             1027  CCTGAGG             
CfoI          108  GCGC                 GCG^C
             1521  GCGC                
DraII        1353  AGGGCCC              [GA]G^G[ACGT]CC[CT]
             1354  GGGCCCT             
Eco81I        775  CCTGAGG              CC^T[ACGT]AGG
             1027  CCTGAGG             
EcoO109I     1353  AGGGCCC              [GA]G^G[ACGT]CC[CT]
             1354  GGGCCCT             
HhaI          108  GCGC                 GCG^C
             1521  GCGC                
HinP1I        108  GCGC                 G^CGC
             1521  GCGC                
MaeIII        821  GTGAC                ^GT[ACGT]AC
              848  GTGAC               
MamI           11  GATCTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              766  GATGGCTATC          
MslI          733  CATGATGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1135  CATTCTGATG          
RcaI          732  TCATGA               T^CATGA
             1898  TCATGA              
ScaI          228  AGTACT               AGT^ACT
              808  AGTACT              
SfcI          125  CTGCAG               C^T[GA][CT]AG
             1280  CTACAG              
TaqI          120  TCGA                 T^CGA
             1728  TCGA                
Tsp45I        821  GTGAC                ^GT[GC]AC
              848  GTGAC               
TthHB8I       120  TCGA                 T^CGA
             1728  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BanII       BbvI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BssKI       BstF5I      BstNI       BstYI       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
ItaI        Ksp632I     MaeI        MaeII       MboI        
MboII       MflI        MnlI        MseI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       Van91I      XhoII       
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