Restriction map for pF1KB4082
 (Linear) (Six-base) MAPSORT of: KIBB4082.seq  Check: 879  from: 1  to: 2682



KIBB4082.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AatII G_ACGT'C

Cuts at:      0     596    2682 
   Size:        596    2086
 
AccI GT'mk_AC

Cuts at:      0     913    1367    2682 
   Size:        913     454    1315

  Fragments arranged by size: 

                1315     913     454
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     908    2024    2682 
   Size:        908    1116     658

  Fragments arranged by size: 

                1116     908     658
 
AfeI AGC'GCT

Cuts at:      0     147     856    2451    2682 
   Size:        147     709    1595     231

  Fragments arranged by size: 

                1595     709     231     147
 
AflIII A'CryG_T

Cuts at:      0    1392    2654    2682 
   Size:       1392    1262      28

  Fragments arranged by size: 

                1392    1262      28
 
AhdI GACnn_n'nnGTC

Cuts at:      0     321    2682 
   Size:        321    2361
 
ApaI G_GGCC'C

Cuts at:      0     357    2682 
   Size:        357    2325
 
ApoI r'AATT_y

Cuts at:      0     601     880    1804    2065    2533    2682 
   Size:        601     279     924     261     468     149

  Fragments arranged by size: 

                 924     601     468     279     261     149
 
AvaI C'yCGr_G

Cuts at:      0     282    2168    2682 
   Size:        282    1886     514

  Fragments arranged by size: 

                1886     514     282
 
AvrII C'CTAG_G

Cuts at:      0     705    2682 
   Size:        705    1977
 
BamHI G'GATC_C

Cuts at:      0    1449    1644    2682 
   Size:       1449     195    1038

  Fragments arranged by size: 

                1449    1038     195
 
BanI G'GyrC_C

Cuts at:      0    1840    2682 
   Size:       1840     842
 
BanII G_rGCy'C

Cuts at:      0     357    1071    2682 
   Size:        357     714    1611

  Fragments arranged by size: 

                1611     714     357
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      36     445    1757    2012    2682 
   Size:         36     409    1312     255     670

  Fragments arranged by size: 

                1312     670     409     255      36
 
BclI T'GATC_A

Cuts at:      0    1925    2682 
   Size:       1925     757
 
BfrBI ATG'CAT

Cuts at:      0     783    2682 
   Size:        783    1899
 
BlpI GC'TnA_GC

Cuts at:      0    1020    2313    2682 
   Size:       1020    1293     369

  Fragments arranged by size: 

                1293    1020     369
 
Bme1580I G_kGCm'C

Cuts at:      0     357    1040    2682 
   Size:        357     683    1642

  Fragments arranged by size: 

                1642     683     357
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     270    2682 
   Size:        270    2412
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1031    1062    2227    2682 
   Size:       1031      31    1165     455

  Fragments arranged by size: 

                1165    1031     455      31
 
Bpu10I CC'TnA_GC

Cuts at:      0    2461    2682 
   Size:       2461     221
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     702    2682 
   Size:        702    1980
 
BsaAI yAC'GTr

Cuts at:      0     222    2682 
   Size:        222    2460
 
BsaHI Gr'CG_yC

Cuts at:      0     593    2682 
   Size:        593    2089
 
BsaWI w'CCGG_w

Cuts at:      0    2050    2682 
   Size:       2050     632
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     990    1020    1685    1715    2682 
   Size:        990      30     665      30     967

  Fragments arranged by size: 

                 990     967     665      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     987    2682 
   Size:        987    1695
 
BseYI C'CCAG_C

Cuts at:      0     426     857    2682 
   Size:        426     431    1825

  Fragments arranged by size: 

                1825     431     426
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     184     343     424    2682 
   Size:        154      30     159      81    2258

  Fragments arranged by size: 

                2258     159     154      81      30
 
BsiHKAI G_wGCw'C

Cuts at:      0      35     143    1071    2682 
   Size:         35     108     928    1611

  Fragments arranged by size: 

                1611     928     108      35
 
BsmI GAATG_Cn'

Cuts at:      0     903    2682 
   Size:        903    1779
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     143     357    1040    1071    2682 
   Size:         35     108     214     683      31    1611

  Fragments arranged by size: 

                1611     683     214     108      35      31
 
BspHI T'CATG_A

Cuts at:      0     119    1574    1595    2682 
   Size:        119    1455      21    1087

  Fragments arranged by size: 

                1455    1087     119      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1712    2682 
   Size:       1712     970
 
BsrDI GCAATG_nn'

Cuts at:      0    1248    2472    2682 
   Size:       1248    1224     210

  Fragments arranged by size: 

                1248    1224     210
 
BsrGI T'GTAC_A

Cuts at:      0    1310    2682 
   Size:       1310    1372
 
BssSI C'ACGA_G

Cuts at:      0    1769    2682 
   Size:       1769     913
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      30    1030    2682 
   Size:         30    1000    1652

  Fragments arranged by size: 

                1652    1000      30
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2520    2682 
   Size:       2520     162
 
BstYI r'GATC_y

Cuts at:      0    1449    1644    2077    2682 
   Size:       1449     195     433     605

  Fragments arranged by size: 

                1449     605     433     195
 
BstZ17I GTA'TAC

Cuts at:      0     914    2682 
   Size:        914    1768
 
Bsu36I CC'TnA_GG

Cuts at:      0     970    2682 
   Size:        970    1712
 
BtsI GCAGTG_nn'

Cuts at:      0     548    2682 
   Size:        548    2134
 
ClaI AT'CG_AT

Cuts at:      0    1148    2682 
   Size:       1148    1534
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     557     592    2682 
   Size:        557      35    2090

  Fragments arranged by size: 

                2090     557      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     664    2682 
   Size:        664    2018
 
EaeI y'GGCC_r

Cuts at:      0       2    2510    2682 
   Size:          2    2508     172

  Fragments arranged by size: 

                2508     172       2
 
EcoICRI GAG'CTC

Cuts at:      0    1069    2682 
   Size:       1069    1613
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     908    1031    1062    2024    2227    2682 
   Size:        908     123      31     962     203     455

  Fragments arranged by size: 

                 962     908     455     203     123      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0     353     354    2039    2682 
   Size:        353       1    1685     643

  Fragments arranged by size: 

                1685     643     353       1
 
EcoRI G'AATT_C

Cuts at:      0     601     880    2533    2682 
   Size:        601     279    1653     149

  Fragments arranged by size: 

                1653     601     279     149
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     666    2214    2682 
   Size:        666    1548     468

  Fragments arranged by size: 

                1548     666     468
 
HaeII r_GCGC'y

Cuts at:      0     149     443     858    2453    2682 
   Size:        149     294     415    1595     229

  Fragments arranged by size: 

                1595     415     294     229     149
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     307     339     500     532    1237    1269    1971    2003 
   Size:        307      32     161      32     705      32     702      32

Cuts at:   2003    2436    2468    2682 
   Size:        433      32     214

  Fragments arranged by size: 

                 705     702     433     307     214     161      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     819    2213    2682 
   Size:        819    1394     469

  Fragments arranged by size: 

                1394     819     469
 
HindIII A'AGCT_T

Cuts at:      0     956    2682 
   Size:        956    1726
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1878    2475    2682 
   Size:       1878     597     207

  Fragments arranged by size: 

                1878     597     207
 
MscI TGG'CCA

Cuts at:      0       4    2512    2682 
   Size:          4    2508     170

  Fragments arranged by size: 

                2508     170       4
 
MslI CAynn'nnrTG

Cuts at:      0     418    1150    1276    2518    2682 
   Size:        418     732     126    1242     164

  Fragments arranged by size: 

                1242     732     418     164     126
 
MspA1I CmG'CkG

Cuts at:      0    2125    2682 
   Size:       2125     557
 
NdeI CA'TA_TG

Cuts at:      0    1302    2034    2682 
   Size:       1302     732     648

  Fragments arranged by size: 

                1302     732     648
 
NsiI A_TGCA'T

Cuts at:      0     785    2682 
   Size:        785    1897
 
NspI r_CATG'y

Cuts at:      0     738    1396    2682 
   Size:        738     658    1286

  Fragments arranged by size: 

                1286     738     658
 
PciI A'CATG_T

Cuts at:      0    1392    2682 
   Size:       1392    1290
 
PfoI T'CCnGG_A

Cuts at:      0     994    2428    2682 
   Size:        994    1434     254

  Fragments arranged by size: 

                1434     994     254
 
PpuMI rG'GwC_Cy

Cuts at:      0    2039    2682 
   Size:       2039     643
 
PspOMI G'GGCC_C

Cuts at:      0     353    2682 
   Size:        353    2329
 
SacI G_AGCT'C

Cuts at:      0    1071    2682 
   Size:       1071    1611
 
SanDI GG'GwC_CC

Cuts at:      0    2039    2682 
   Size:       2039     643
 
SmlI C'TyrA_G

Cuts at:      0     282     717    2682 
   Size:        282     435    1965

  Fragments arranged by size: 

                1965     435     282
 
SspI AAT'ATT

Cuts at:      0    1107    1814    2682 
   Size:       1107     707     868

  Fragments arranged by size: 

                1107     868     707
 
StyI C'CwwG_G

Cuts at:      0     705    1835    2183    2420    2682 
   Size:        705    1130     348     237     262

  Fragments arranged by size: 

                1130     705     348     262     237
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     849    1828    2602    2682 
   Size:        849     979     774      80

  Fragments arranged by size: 

                 979     849     774      80
 
TatI w'GTAC_w

Cuts at:      0    1086    1310    2225    2682 
   Size:       1086     224     915     457

  Fragments arranged by size: 

                1086     915     457     224
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     220     252     990    1022    1425    1457    2682 
   Size:        220      32     738      32     403      32    1225

  Fragments arranged by size: 

                1225     738     403     220      32      32      32
 
XhoI C'TCGA_G

Cuts at:      0     282    2682 
   Size:        282    2400
 
XmnI GAAnn'nnTTC

Cuts at:      0     202    2682 
   Size:        202    2480
 
ZraI GAC'GTC

Cuts at:      0     594    2682 
   Size:        594    2088
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI   AflIII     AhdI     ApaI     ApoI
     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BclI    BfrBI
     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstXI    BstYI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DrdI     EaeI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII
     MmeI     MscI     MslI   MspA1I     NdeI     NsiI     NspI     PciI
     PfoI    PpuMI   PspOMI     SacI    SanDI     SmlI     SspI     StyI
    TaqII     TatI     TstI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII     AgeI     AleI     AlfI     AloI
    AlwNI    ApaLI     AscI     AseI    AsiSI     BaeI     BbeI    BbvCI
     BcgI    BciVI     BglI    BglII    BmgBI     BmtI     BplI     BsaI
    BsaBI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI   BssHII
    BstBI   BstEII     BtgI    BtgZI     DraI   DraIII     EagI     EarI
     EciI    EcoNI    EcoRV     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI  Tth111I     XbaI     XcmI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com