Restriction map for pF1KB4058
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# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflIII      AgeI        AhdI        
AlwNI       Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BanI        BbeI        BcgI        
BciVI       BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       FseI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp509I     Tth111I     XcmI        XhoI        
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII          96  CGCG                 CG^CG
AflII        1513  CTTAAG               C^TTAAG
Alw44I       1207  GTGCAC               G^TGCAC
AocI         1332  CCTGAGG              CC^T[ACGT]AGG
ApaI          350  GGGCCC               GGGCC^C
ApaLI        1207  GTGCAC               G^TGCAC
AspHI        1207  GTGCAC               G[AT]GC[AT]^C
AvaI          886  CCCGAG               C^[CT]CG[GA]G
BalI          797  TGGCCA               TGG^CCA
BbrPI        1204  CACGTG               CAC^GTG
BbvCI          31  CCTCAGC              CCTCAGC(-5/-2)
BclI         1098  TGATCA               T^GATCA
BfrI         1513  CTTAAG               C^TTAAG
BsaAI        1204  CACGTG               [CT]AC^GT[GA]
BsaI         1426  GAGACC               GGTCTC(1/5)
BsaWI        1499  TCCGGT               [AT]^CCGG[AT]
BsiHKAI      1207  GTGCAC               G[AT]GC[AT]^C
BsmAI        1426  GAGAC                GTCTC(1/5)
BsoBI         886  CCCGAG               C^[CT]CG[GA]G
BsrBI          11  CCGCTC               CCGCTC(-3/-3)
BsrDI        1140  CATTGC               GCAATG(2/0)
BsrFI          60  GCCGGT               [GA]^CCGG[CT]
BstAPI        919  GCAGAGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          96  CGCG                 CG^CG
Bsu36I       1332  CCTGAGG              CC^T[ACGT]AGG
CfoI          753  GCGC                 GCG^C
Cfr10I         60  GCCGGT               [GA]^CCGG[CT]
DsaI          416  CCATGG               C^C[GA][CT]GG
EaeI          797  TGGCCA               [CT]^GGCC[GA]
Eco81I       1332  CCTGAGG              CC^T[ACGT]AGG
EcoNI         270  CCTTCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1098  TGATCA               T^GATCA
HaeII         752  AGCGCC               [GA]GCGC^[CT]
HhaI          753  GCGC                 GCG^C
HinP1I        753  GCGC                 G^CGC
HphI         1552  GGTGA                GGTGA(8/7)
MluNI         797  TGGCCA               TGG^CCA
MscI          797  TGGCCA               TGG^CCA
MvnI           96  CGCG                 CG^CG
NcoI          416  CCATGG               C^CATGG
NspI           39  GCATGC               [GA]CATG^[CT]
PmaCI        1204  CACGTG               CAC^GTG
PmlI         1204  CACGTG               CAC^GTG
ScaI          773  AGTACT               AGT^ACT
SphI           39  GCATGC               GCATG^C
TfiI          455  GATTC                G^A[AT]TC
XbaI         1521  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BbsI         1199  GTCTTC               GAAGAC(2/6)
             1487  GTCTTC              
BpuAI        1199  GTCTTC               GAAGAC(2/6)
             1487  GTCTTC              
BsgI          565  CTGCAC               GTGCAG(16/14)
              738  CTGCAC              
EcoT22I        82  ATGCAT               ATGCA^T
             1415  ATGCAT              
HgaI           97  GCGTC                GACGC(5/10)
              834  GCGTC               
MaeII        1205  ACGT                 A^CGT
             1485  ACGT                
NsiI           82  ATGCAT               ATGCA^T
             1415  ATGCAT              
PflMI         331  CCATCCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1599  CCATCAACTGG         
PleI          235  GACTC                GAGTC(4/5)
              281  GAGTC               
PvuII         660  CAGCTG               CAG^CTG
             1025  CAGCTG              
SmlI         1513  CTTAAG               C^T[CT][GA]AG
             1588  CTTGAG              
TaqI         1173  TCGA                 T^CGA
             1565  TCGA                
TthHB8I      1173  TCGA                 T^CGA
             1565  TCGA                
Van91I        331  CCATCCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1599  CCATCAACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BamHI       BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        Tru9I       
TseI        Tsp45I      TspRI       XhoII       
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