Restriction map for
pF1KB4031
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BspDI BspEI BsrBI
BsrDI BsrFI BssHII Bst1107I BstBI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HapII HhaI HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1228 GTAGAC GT^[AC][GT]AC
AccII 5 CGCG CG^CG
AcyI 614 GACGCC G[GA]^CG[CT]C
AflII 204 CTTAAG C^TTAAG
AlwNI 1645 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 484 CCTCAGG CC^T[ACGT]AGG
ApaI 698 GGGCCC GGGCC^C
AseI 1240 ATTAAT AT^TAAT
AsnI 1240 ATTAAT AT^TAAT
AvaI 892 CCCGAG C^[CT]CG[GA]G
BanII 698 GGGCCC G[GA]GC[CT]^C
BfrI 204 CTTAAG C^TTAAG
BglI 2359 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 614 GACGCC G[GA]^CG[CT]C
BsgI 998 CTGCAC GTGCAG(16/14)
BsoBI 892 CCCGAG C^[CT]CG[GA]G
BspMI 1444 ACCTGC ACCTGC(4/8)
BssSI 1886 CTCGTG CACGAG(-5/-1)
BstAPI 795 GCACAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2286 GGTGACC G^GT[ACGT]ACC
BstPI 2286 GGTGACC G^GT[ACGT]ACC
BstUI 5 CGCG CG^CG
Bsu36I 484 CCTCAGG CC^T[ACGT]AGG
DraI 1522 TTTAAA TTT^AAA
DraII 897 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 2212 CCATGG C^C[GA][CT]GG
EaeI 2336 TGGCCG [CT]^GGCC[GA]
Eco81I 484 CCTCAGG CC^T[ACGT]AGG
EcoO109I 897 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2286 GGTGACC G^GT[ACGT]ACC
EcoT22I 512 ATGCAT ATGCA^T
HgaI 614 GACGC GACGC(5/10)
Hin1I 614 GACGCC G[GA]^CG[CT]C
HincII 329 GTCAAC GT[CT]^[GA]AC
HindII 329 GTCAAC GT[CT]^[GA]AC
MspA1I 2143 CAGCGG C[AC]G^C[GT]G
MvnI 5 CGCG CG^CG
NcoI 2212 CCATGG C^CATGG
NsiI 512 ATGCAT ATGCA^T
PpuMI 897 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1240 ATTAAT AT^TAAT
SbfI 2361 CCTGCAGG CCTGCA^GG
SpeI 1809 ACTAGT A^CTAGT
Sse8387I 2361 CCTGCAGG CCTGCA^GG
XbaI 1356 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 1752 ACATGT A^C[GA][CT]GT
1950 ACATGT
Asp700I 2077 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2121 GAATCTTTTC
AspHI 1691 GTGCTC G[AT]GC[AT]^C
2167 GAGCAC
BbsI 987 GAAGAC GAAGAC(2/6)
1714 GTCTTC
BglII 200 AGATCT A^GATCT
2246 AGATCT
BpmI 1779 CTGGAG CTGGAG(16/14)
2293 CTGGAG
BpuAI 987 GAAGAC GAAGAC(2/6)
1714 GTCTTC
BseRI 821 CTCCTC GAGGAG(10/8)
1123 CTCCTC
BsiHKAI 1691 GTGCTC G[AT]GC[AT]^C
2167 GAGCAC
BsmI 279 GAATGC GAATGC(1/-1)
1556 GCATTC
BspHI 1707 TCATGA T^CATGA
2153 TCATGA
BspLU11I 1752 ACATGT A^CATGT
1950 ACATGT
EcoT14I 853 CCAAGG C^C[AT][AT]GG
2212 CCATGG
MslI 1099 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1101 CATTGATGTG
RcaI 1707 TCATGA T^CATGA
2153 TCATGA
SmlI 204 CTTAAG C^T[CT][GA]AG
2062 CTTGAG
StyI 853 CCAAGG C^C[AT][AT]GG
2212 CCATGG
XmnI 2077 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2121 GAATCTTTTC
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII