Restriction map for pF1KB4031
 (Linear) (Six-base) MAPSORT of: KIBB4031.seq  Check: 8654  from: 1  to: 2370



KIBB4031.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AccI GT'mk_AC

Cuts at:      0    1229    2370 
   Size:       1229    1141
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     849     851    1536    1671    1859    1978    2068 
   Size:        101     748       2     685     135     188     119      90

Cuts at:   2068    2370 
   Size:        302

  Fragments arranged by size: 

                 748     685     302     188     135     119     101      90
                   2
 
AflII C'TTAA_G

Cuts at:      0     204    2370 
   Size:        204    2166
 
AflIII A'CryG_T

Cuts at:      0    1752    1950    2370 
   Size:       1752     198     420

  Fragments arranged by size: 

                1752     420     198
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     728     760    2370 
   Size:        728      32    1610

  Fragments arranged by size: 

                1610     728      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1650    2370 
   Size:       1650     720
 
ApaI G_GGCC'C

Cuts at:      0     702    2370 
   Size:        702    1668
 
ApoI r'AATT_y

Cuts at:      0     307     450    1916    2370 
   Size:        307     143    1466     454

  Fragments arranged by size: 

                1466     454     307     143
 
AseI AT'TA_AT

Cuts at:      0    1241    2370 
   Size:       1241    1129
 
AvaI C'yCGr_G

Cuts at:      0     892    2370 
   Size:        892    1478
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     614     647    2370 
   Size:        614      33    1723

  Fragments arranged by size: 

                1723     614      33
 
BanII G_rGCy'C

Cuts at:      0     702    2370 
   Size:        702    1668
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     994    1707    2370 
   Size:        994     713     663

  Fragments arranged by size: 

                 994     713     663
 
BfrBI ATG'CAT

Cuts at:      0     514    2370 
   Size:        514    1856
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2365    2370 
   Size:       2365       5
 
BglII A'GATC_T

Cuts at:      0     200    2246    2370 
   Size:        200    2046     124

  Fragments arranged by size: 

                2046     200     124
 
Bme1580I G_kGCm'C

Cuts at:      0     702    2361    2370 
   Size:        702    1659       9

  Fragments arranged by size: 

                1659     702       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1800    2314    2370 
   Size:       1800     514      56

  Fragments arranged by size: 

                1800     514      56
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2083    2370 
   Size:       2083     287
 
BsaHI Gr'CG_yC

Cuts at:      0     615    2370 
   Size:        615    1755
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     296     326     804     834    2370 
   Size:        296      30     478      30    1536

  Fragments arranged by size: 

                1536     478     296      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     812    1114    2370 
   Size:        812     302    1256

  Fragments arranged by size: 

                1256     812     302
 
BseYI C'CCAG_C

Cuts at:      0    1286    2370 
   Size:       1286    1084
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     983    2370 
   Size:        983    1387
 
BsiHKAI G_wGCw'C

Cuts at:      0    1695    2171    2370 
   Size:       1695     476     199

  Fragments arranged by size: 

                1695     476     199
 
BsmI GAATG_Cn'

Cuts at:      0     285    1556    2370 
   Size:        285    1271     814

  Fragments arranged by size: 

                1271     814     285
 
Bsp1286I G_dGCh'C

Cuts at:      0     702    1695    2171    2361    2370 
   Size:        702     993     476     190       9

  Fragments arranged by size: 

                 993     702     476     190       9
 
BspHI T'CATG_A

Cuts at:      0    1707    2153    2370 
   Size:       1707     446     217

  Fragments arranged by size: 

                1707     446     217
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1453    2370 
   Size:       1453     917
 
BsrGI T'GTAC_A

Cuts at:      0     217    1112    1641    2370 
   Size:        217     895     529     729

  Fragments arranged by size: 

                 895     729     529     217
 
BssSI C'ACGA_G

Cuts at:      0    1886    2370 
   Size:       1886     484
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     801    2370 
   Size:        801    1569
 
BstEII G'GTnAC_C

Cuts at:      0    2286    2370 
   Size:       2286      84
 
BstYI r'GATC_y

Cuts at:      0     200    1031    1317    1865    1961    2246    2370 
   Size:        200     831     286     548      96     285     124

  Fragments arranged by size: 

                 831     548     286     285     200     124      96
 
Bsu36I CC'TnA_GG

Cuts at:      0     485    2370 
   Size:        485    1885
 
BtgI C'CryG_G

Cuts at:      0    2212    2370 
   Size:       2212     158
 
BtsI GCAGTG_nn'

Cuts at:      0     787     954    2046    2309    2370 
   Size:        787     167    1092     263      61

  Fragments arranged by size: 

                1092     787     263     167      61
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     282     317    2370 
   Size:        282      35    2053

  Fragments arranged by size: 

                2053     282      35
 
DraI TTT'AAA

Cuts at:      0    1524    2370 
   Size:       1524     846
 
EaeI y'GGCC_r

Cuts at:      0    2336    2370 
   Size:       2336      34
 
EarI CTCTTCn'nnn_

Cuts at:      0     671     938    1487    1878    2311    2370 
   Size:        671     267     549     391     433      59

  Fragments arranged by size: 

                 671     549     433     391     267      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     849     851    1536    1671    1800    1859    1978 
   Size:        101     748       2     685     135     129      59     119

Cuts at:   1978    2068    2314    2370 
   Size:         90     246      56

  Fragments arranged by size: 

                 748     685     246     135     129     119     101      90
                  59      56       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     898    2370 
   Size:        898    1472
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     196     967    1007    1053    2011    2370 
   Size:        196     771      40      46     958     359

  Fragments arranged by size: 

                 958     771     359     196      46      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1773    1805    2370 
   Size:       1773      32     565

  Fragments arranged by size: 

                1773     565      32
 
HincII GTy'rAC

Cuts at:      0     331    2370 
   Size:        331    2039
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     519    1261    2370 
   Size:        249     270     742    1109

  Fragments arranged by size: 

                1109     742     270     249
 
MslI CAynn'nnrTG

Cuts at:      0    1103    1105    2370 
   Size:       1103       2    1265

  Fragments arranged by size: 

                1265    1103       2
 
MspA1I CmG'CkG

Cuts at:      0    2145    2370 
   Size:       2145     225
 
NcoI C'CATG_G

Cuts at:      0    2212    2370 
   Size:       2212     158
 
NsiI A_TGCA'T

Cuts at:      0     516    2370 
   Size:        516    1854
 
NspI r_CATG'y

Cuts at:      0    1200    1756    1954    2370 
   Size:       1200     556     198     416

  Fragments arranged by size: 

                1200     556     416     198
 
PasI CC'CwG_GG

Cuts at:      0     533    2370 
   Size:        533    1837
 
PciI A'CATG_T

Cuts at:      0    1752    1950    2370 
   Size:       1752     198     420

  Fragments arranged by size: 

                1752     420     198
 
PfoI T'CCnGG_A

Cuts at:      0    1485    2370 
   Size:       1485     885
 
PpuMI rG'GwC_Cy

Cuts at:      0     898    2370 
   Size:        898    1472
 
PsiI TTA'TAA

Cuts at:      0     362    2370 
   Size:        362    2008
 
PspOMI G'GGCC_C

Cuts at:      0     698    2370 
   Size:        698    1672
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1337    1369    2370 
   Size:       1337      32    1001

  Fragments arranged by size: 

                1337    1001      32
 
PstI C_TGCA'G

Cuts at:      0     794    2243    2366    2370 
   Size:        794    1449     123       4

  Fragments arranged by size: 

                1449     794     123       4
 
SbfI CC_TGCA'GG

Cuts at:      0    2366    2370 
   Size:       2366       4
 
SfcI C'TryA_G

Cuts at:      0     790    2239    2254    2362    2370 
   Size:        790    1449      15     108       8

  Fragments arranged by size: 

                1449     790     108      15       8
 
SmlI C'TyrA_G

Cuts at:      0     204    2062    2370 
   Size:        204    1858     308

  Fragments arranged by size: 

                1858     308     204
 
SpeI A'CTAG_T

Cuts at:      0    1809    2370 
   Size:       1809     561
 
StyI C'CwwG_G

Cuts at:      0     853    2212    2370 
   Size:        853    1359     158

  Fragments arranged by size: 

                1359     853     158
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2179    2370 
   Size:       2179     191
 
TatI w'GTAC_w

Cuts at:      0     217    1112    1641    1806    2279    2370 
   Size:        217     895     529     165     473      91

  Fragments arranged by size: 

                 895     529     473     217     165      91
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     873     905     978    1010    2370 
   Size:        873      32      73      32    1360

  Fragments arranged by size: 

                1360     873      73      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1356    2370 
   Size:       1356    1014
 
XmnI GAAnn'nnTTC

Cuts at:      0    2081    2125    2370 
   Size:       2081      44     245

  Fragments arranged by size: 

                2081     245      44
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AloI    AlwNI     ApaI     ApoI
     AseI     AvaI     BaeI    BanII     BbsI    BfrBI     BglI    BglII
 Bme1580I     BpmI    BpuEI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrGI    BssSI   BstAPI
   BstEII    BstYI   Bsu36I     BtgI     BtsI    CspCI     DraI     EaeI
     EarI  Eco57MI EcoO109I     FalI    Hin4I   HincII     MmeI     MslI
   MspA1I     NcoI     NsiI     NspI     PasI     PciI     PfoI    PpuMI
     PsiI   PspOMI     PsrI     PstI     SbfI     SfcI     SmlI     SpeI
     StyI    TaqII     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AlfI    ApaLI     AscI    AsiSI    AvrII    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BstBI    BstXI
  BstZ17I    BtgZI     ClaI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI    PflMI     PmeI
     PmlI     PpiI    PshAI    PspXI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XcmI     XhoI     XmaI     ZraI
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