Restriction map for pF1KB4018
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BglI        BlnI        BlpI        Bpu1102I    
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsoBI       BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRV       
FbaI        FseI        FspI        HaeII       HgaI        
KasI        KpnI        KspI        MluI        MroI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NotI        NruI        NspV        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        SwaI        
Tsp45I      Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII          85  GACGTC               GACGT^C
AccI           88  GTCTAC               GT^[AC][GT]AC
AcyI           85  GACGTC               G[GA]^CG[CT]C
AflII        1023  CTTAAG               C^TTAAG
AflIII       1221  ACACGT               A^C[GA][CT]GT
AocI         1469  CCTTAGG              CC^T[ACGT]AGG
Asp700I       504  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         316  GGACC                G^G[AT]CC
BalI          999  TGGCCA               TGG^CCA
BcnI            7  CCCGG                CC^[GC]GG
BfrI         1023  CTTAAG               C^TTAAG
BsaAI         307  CACGTA               [CT]AC^GT[GA]
BsaHI          85  GACGTC               G[GA]^CG[CT]C
BsmBI        2263  GAGACG               CGTCTC(1/5)
BstXI        1494  CCAGTTAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1469  CCTTAGG              CC^T[ACGT]AGG
CfoI           60  GCGC                 GCG^C
DraIII        742  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          999  TGGCCA               [CT]^GGCC[GA]
EarI         2286  CTCTTC               CTCTTC(1/4)
Eco81I       1469  CCTTAGG              CC^T[ACGT]AGG
EcoRI         941  GAATTC               G^AATTC
EcoT22I      1342  ATGCAT               ATGCA^T
HhaI           60  GCGC                 GCG^C
Hin1I          85  GACGTC               G[GA]^CG[CT]C
HinP1I         60  GCGC                 G^CGC
HpaI          106  GTTAAC               GTT^AAC
Ksp632I      2286  CTCTTC               CTCTTC(1/4)
MluNI         999  TGGCCA               TGG^CCA
MscI          999  TGGCCA               TGG^CCA
NciI            7  CCCGG                CC^[GC]GG
NheI         2490  GCTAGC               G^CTAGC
NsiI         1342  ATGCAT               ATGCA^T
NspI         1636  GCATGT               [GA]CATG^[CT]
PacI         2128  TTAATTAA             TTAAT^TAA
PleI         1179  GAGTC                GAGTC(4/5)
PshAI         317  GACCTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          361  CTGCAG               CTGCA^G
PvuII         548  CAGCTG               CAG^CTG
SacI         1485  GAGCTC               GAGCT^C
ScaI         1718  AGTACT               AGT^ACT
SfcI          361  CTGCAG               C^T[GA][CT]AG
SspI          214  AATATT               AAT^ATT
XmnI          504  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AspHI        1485  GAGCTC               G[AT]GC[AT]^C
             2069  GAGCAC              
AspI          831  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2593  GACAGGGTC           
BanII        1398  GAGCCC               G[GA]GC[CT]^C
             1485  GAGCTC              
BglII        1108  AGATCT               A^GATCT
             2530  AGATCT              
BpmI          536  CTCCAG               CTGGAG(16/14)
             2344  CTGGAG              
BsgI          473  CTGCAC               GTGCAG(16/14)
              172  GTGCAG              
BsiHKAI      1485  GAGCTC               G[AT]GC[AT]^C
             2069  GAGCAC              
BsmI         2510  GAATGC               GAATGC(1/-1)
             1344  GCATTC              
Bsp1407I     1810  TGTACA               T^GTACA
             2651  TGTACA              
BsrGI        1810  TGTACA               T^GTACA
             2651  TGTACA              
Cac8I        1838  GCTTGC               GC[ACGT]^[ACGT]GC
             2490  GCTAGC              
Cfr13I        316  GGACC                G^G[ACGT]CC
             2049  GGGCC               
HapII           8  CCGG                 C^CGG
               27  CCGG                
HincII        106  GTTAAC               GT[CT]^[GA]AC
             1526  GTCAAC              
HindII        106  GTTAAC               GT[CT]^[GA]AC
             1526  GTCAAC              
HindIII       253  AAGCTT               A^AGCTT
             1859  AAGCTT              
HpaII           8  CCGG                 C^CGG
               27  CCGG                
MfeI          491  CAATTG               C^AATTG
             1833  CAATTG              
MspA1I         69  CAGCGG               C[AC]G^C[GT]G
              548  CAGCTG              
MspI            8  CCGG                 C^CGG
               27  CCGG                
MunI          491  CAATTG               C^AATTG
             1833  CAATTG              
NdeI          341  CATATG               CA^TATG
             1627  CATATG              
PflFI         831  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2593  GACAGGGTC           
Sau96I        316  GGACC                G^G[ACGT]CC
             2049  GGGCC               
SmlI         1023  CTTAAG               C^T[CT][GA]AG
             1186  CTCAAG              
SspBI        1810  TGTACA               T^GTACA
             2651  TGTACA              
Tth111I       831  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2593  GACAGGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BanI        BbsI        BbvI        BfaI        
BmyI        BpuAI       BsaBI       BsaJI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspHI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
DdeI        DpnII       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RcaI        RsaI        Sau3AI      ScrFI       
SfaNI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com