Restriction map for pF1KB3935
 (Linear) (Six-base) MAPSORT of: KIBB3935.seq  Check: 7007  from: 1  to: 1266



KIBB3935.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1059    1266 
   Size:       1059     207
 
Acc65I G'GTAC_C

Cuts at:      0     642    1266 
   Size:        642     624
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     421     919     991    1266 
   Size:        148     273     498      72     275

  Fragments arranged by size: 

                 498     275     273     148      72
 
AlwNI CAG_nnn'CTG

Cuts at:      0     477    1266 
   Size:        477     789
 
ApoI r'AATT_y

Cuts at:      0     158     577     727    1266 
   Size:        158     419     150     539

  Fragments arranged by size: 

                 539     419     158     150
 
BanI G'GyrC_C

Cuts at:      0     642    1266 
   Size:        642     624
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     340     609    1266 
   Size:        340     269     657

  Fragments arranged by size: 

                 657     340     269
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      45      79    1266 
   Size:         45      34    1187

  Fragments arranged by size: 

                1187      45      34
 
BfrBI ATG'CAT

Cuts at:      0     115    1266 
   Size:        115    1151
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1252    1266 
   Size:       1252      14
 
Bme1580I G_kGCm'C

Cuts at:      0     529    1266 
   Size:        529     737
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     436    1203    1266 
   Size:        436     767      63

  Fragments arranged by size: 

                 767     436      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     904    1266 
   Size:        116     788     362

  Fragments arranged by size: 

                 788     362     116
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340    1266 
   Size:        340     926
 
BsaAI yAC'GTr

Cuts at:      0     543     849    1266 
   Size:        543     306     417

  Fragments arranged by size: 

                 543     417     306
 
BsaBI GATnn'nnATC

Cuts at:      0     695    1266 
   Size:        695     571
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     433    1246    1266 
   Size:        433     813      20

  Fragments arranged by size: 

                 813     433      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1087    1266 
   Size:       1087     179
 
BsmI GAATG_Cn'

Cuts at:      0     303    1266 
   Size:        303     963
 
Bsp1286I G_dGCh'C

Cuts at:      0     529    1266 
   Size:        529     737
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     229     794    1059    1215    1266 
   Size:        229     565     265     156      51

  Fragments arranged by size: 

                 565     265     229     156      51
 
BsrDI GCAATG_nn'

Cuts at:      0      78     163    1266 
   Size:         78      85    1103

  Fragments arranged by size: 

                1103      85      78
 
BsrFI r'CCGG_y

Cuts at:      0    1150    1266 
   Size:       1150     116
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1221    1266 
   Size:       1221      45
 
BtgI C'CryG_G

Cuts at:      0     172    1266 
   Size:        172    1094
 
DraI TTT'AAA

Cuts at:      0     732    1266 
   Size:        732     534
 
EaeI y'GGCC_r

Cuts at:      0     759    1184    1253    1266 
   Size:        759     425      69      13

  Fragments arranged by size: 

                 759     425      69      13
 
EarI CTCTTCn'nnn_

Cuts at:      0     410     800    1266 
   Size:        410     390     466

  Fragments arranged by size: 

                 466     410     390
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     148     421     904     919     991    1266 
   Size:        116      32     273     483      15      72     275

  Fragments arranged by size: 

                 483     275     273     116      72      32      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      98    1266 
   Size:         98    1168
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24     351    1266 
   Size:         24     327     915

  Fragments arranged by size: 

                 915     327      24
 
EcoRV GAT'ATC

Cuts at:      0     828    1266 
   Size:        828     438
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29     330     362     688     720    1266 
   Size:         29     301      32     326      32     546

  Fragments arranged by size: 

                 546     326     301      32      32      29
 
KpnI G_GTAC'C

Cuts at:      0     646    1266 
   Size:        646     620
 
MscI TGG'CCA

Cuts at:      0     761    1186    1266 
   Size:        761     425      80

  Fragments arranged by size: 

                 761     425      80
 
MslI CAynn'nnrTG

Cuts at:      0     307    1266 
   Size:        307     959
 
MspA1I CmG'CkG

Cuts at:      0     474    1266 
   Size:        474     792
 
NruI TCG'CGA

Cuts at:      0      55    1266 
   Size:         55    1211
 
NsiI A_TGCA'T

Cuts at:      0     117    1266 
   Size:        117    1149
 
NspI r_CATG'y

Cuts at:      0    1182    1266 
   Size:       1182      84
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     477    1266 
   Size:        477     789
 
PfoI T'CCnGG_A

Cuts at:      0      93    1266 
   Size:         93    1173
 
PshAI GACnn'nnGTC

Cuts at:      0     589     874    1170    1266 
   Size:        589     285     296      96

  Fragments arranged by size: 

                 589     296     285      96
 
PstI C_TGCA'G

Cuts at:      0    1226    1266 
   Size:       1226      40
 
PvuII CAG'CTG

Cuts at:      0     474    1266 
   Size:        474     792
 
SapI GCTCTTCn'nnn_

Cuts at:      0     800    1266 
   Size:        800     466
 
SbfI CC_TGCA'GG

Cuts at:      0    1226    1266 
   Size:       1226      40
 
SfcI C'TryA_G

Cuts at:      0    1222    1266 
   Size:       1222      44
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1150    1266 
   Size:       1150     116
 
SmlI C'TyrA_G

Cuts at:      0     355    1266 
   Size:        355     911
 
SnaBI TAC'GTA

Cuts at:      0     849    1266 
   Size:        849     417
 
StyI C'CwwG_G

Cuts at:      0     512    1266 
   Size:        512     754
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     649     811     904    1266 
   Size:        649     162      93     362

  Fragments arranged by size: 

                 649     362     162      93
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     519     551    1266 
   Size:        519      32     715

  Fragments arranged by size: 

                 715     519      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     170    1266 
   Size:        170    1096
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AlwNI     ApoI     BanI     BbsI     BcgI
    BfrBI     BglI Bme1580I     BmrI     BpmI    BpuEI    BsaAI    BsaBI
    BseRI     BsgI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI   BstAPI
     BtgI     DraI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRV
    Hin4I     KpnI     MscI     MslI   MspA1I     NruI     NsiI     NspI
    PflMI     PfoI    PshAI     PstI    PvuII     SapI     SbfI     SfcI
    SgrAI     SmlI    SnaBI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI    BciVI
     BclI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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