Restriction map for pF1KB3797
 (Linear) (Six-base) MAPSORT of: KIBB3797.seq  Check: 8293  from: 1  to: 1422



KIBB3797.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

Acc65I G'GTAC_C

Cuts at:      0     497    1422 
   Size:        497     925
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     794    1422 
   Size:        245     549     628

  Fragments arranged by size: 

                 628     549     245
 
AflII C'TTAA_G

Cuts at:      0     712    1422 
   Size:        712     710
 
AflIII A'CryG_T

Cuts at:      0     917    1260    1422 
   Size:        917     343     162

  Fragments arranged by size: 

                 917     343     162
 
ApaI G_GGCC'C

Cuts at:      0    1004    1422 
   Size:       1004     418
 
ApaLI G'TGCA_C

Cuts at:      0     388    1422 
   Size:        388    1034
 
AseI AT'TA_AT

Cuts at:      0     797    1422 
   Size:        797     625
 
AvaI C'yCGr_G

Cuts at:      0    1111    1422 
   Size:       1111     311
 
BanI G'GyrC_C

Cuts at:      0     348     436     497    1422 
   Size:        348      88      61     925

  Fragments arranged by size: 

                 925     348      88      61
 
BanII G_rGCy'C

Cuts at:      0    1004    1422 
   Size:       1004     418
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     933    1422 
   Size:        933     489
 
BbvCI CC'TCA_GC

Cuts at:      0     700    1422 
   Size:        700     722
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     571     605    1422 
   Size:        571      34     817

  Fragments arranged by size: 

                 817     571      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     538    1422 
   Size:        538     884
 
Bme1580I G_kGCm'C

Cuts at:      0     392    1004    1422 
   Size:        392     612     418

  Fragments arranged by size: 

                 612     418     392
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1366    1422 
   Size:       1366      56
 
BmtI G_CTAG'C

Cuts at:      0    1183    1422 
   Size:       1183     239
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291    1179    1422 
   Size:        291     888     243

  Fragments arranged by size: 

                 888     291     243
 
Bpu10I CC'TnA_GC

Cuts at:      0     700    1422 
   Size:        700     722
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     147     789    1154    1422 
   Size:        147     642     365     268

  Fragments arranged by size: 

                 642     365     268     147
 
BsaBI GATnn'nnATC

Cuts at:      0     769    1422 
   Size:        769     653
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     386     416    1422 
   Size:        386      30    1006

  Fragments arranged by size: 

                1006     386      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1302    1422 
   Size:       1302     120
 
BseYI C'CCAG_C

Cuts at:      0    1216    1422 
   Size:       1216     206
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     504    1422 
   Size:        504     918
 
BsiHKAI G_wGCw'C

Cuts at:      0     392    1108    1422 
   Size:        392     716     314

  Fragments arranged by size: 

                 716     392     314
 
BsmI GAATG_Cn'

Cuts at:      0    1344    1422 
   Size:       1344      78
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     106    1284    1422 
   Size:        106    1178     138

  Fragments arranged by size: 

                1178     138     106
 
Bsp1286I G_dGCh'C

Cuts at:      0     392    1004    1108    1422 
   Size:        392     612     104     314

  Fragments arranged by size: 

                 612     392     314     104
 
BsrDI GCAATG_nn'

Cuts at:      0     200    1422 
   Size:        200    1222
 
Bsu36I CC'TnA_GG

Cuts at:      0     490    1285    1422 
   Size:        490     795     137

  Fragments arranged by size: 

                 795     490     137
 
BtgI C'CryG_G

Cuts at:      0     176    1422 
   Size:        176    1246
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     596    1422 
   Size:        596     826
 
ClaI AT'CG_AT

Cuts at:      0     212    1422 
   Size:        212    1210
 
DraI TTT'AAA

Cuts at:      0     308    1422 
   Size:        308    1114
 
DraIII CAC_nnn'GTG

Cuts at:      0     396    1422 
   Size:        396    1026
 
EaeI y'GGCC_r

Cuts at:      0    1130    1239    1422 
   Size:       1130     109     183

  Fragments arranged by size: 

                1130     183     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     291     794    1179    1422 
   Size:        245      46     503     385     243

  Fragments arranged by size: 

                 503     385     245     243      46
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     687    1422 
   Size:        687     735
 
EcoO109I rG'GnC_Cy

Cuts at:      0     169    1000    1001    1422 
   Size:        169     831       1     421

  Fragments arranged by size: 

                 831     421     169       1
 
FspI TGC'GCA

Cuts at:      0      71    1422 
   Size:         71    1351
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     364     396     443     475    1339    1371    1422 
   Size:        364      32      47      32     864      32      51

  Fragments arranged by size: 

                 864     364      51      47      32      32      32
 
HincII GTy'rAC

Cuts at:      0     978    1071    1422 
   Size:        978      93     351

  Fragments arranged by size: 

                 978     351      93
 
HindIII A'AGCT_T

Cuts at:      0     709    1422 
   Size:        709     713
 
KpnI G_GTAC'C

Cuts at:      0     501    1422 
   Size:        501     921
 
MscI TGG'CCA

Cuts at:      0    1132    1422 
   Size:       1132     290
 
MslI CAynn'nnrTG

Cuts at:      0     214     431     916    1422 
   Size:        214     217     485     506

  Fragments arranged by size: 

                 506     485     217     214
 
MspA1I CmG'CkG

Cuts at:      0      61     472     814    1024    1422 
   Size:         61     411     342     210     398

  Fragments arranged by size: 

                 411     398     342     210      61
 
NheI G'CTAG_C

Cuts at:      0    1179    1422 
   Size:       1179     243
 
NspI r_CATG'y

Cuts at:      0     753     921    1264    1422 
   Size:        753     168     343     158

  Fragments arranged by size: 

                 753     343     168     158
 
PciI A'CATG_T

Cuts at:      0     917    1260    1422 
   Size:        917     343     162

  Fragments arranged by size: 

                 917     343     162
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     163    1422 
   Size:        163    1259
 
PfoI T'CCnGG_A

Cuts at:      0     875    1422 
   Size:        875     547
 
PpuMI rG'GwC_Cy

Cuts at:      0     169    1422 
   Size:        169    1253
 
PshAI GACnn'nnGTC

Cuts at:      0     146    1422 
   Size:        146    1276
 
PspOMI G'GGCC_C

Cuts at:      0    1000    1422 
   Size:       1000     422
 
PvuII CAG'CTG

Cuts at:      0     472     814    1024    1422 
   Size:        472     342     210     398

  Fragments arranged by size: 

                 472     398     342     210
 
SmlI C'TyrA_G

Cuts at:      0     712    1111    1422 
   Size:        712     399     311

  Fragments arranged by size: 

                 712     399     311
 
StyI C'CwwG_G

Cuts at:      0     524    1422 
   Size:        524     898
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     336     883    1422 
   Size:        336     547     539

  Fragments arranged by size: 

                 547     539     336
 
TatI w'GTAC_w

Cuts at:      0     254    1422 
   Size:        254    1168
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     213    1422 
   Size:        213    1209
 
XhoI C'TCGA_G

Cuts at:      0    1111    1422 
   Size:       1111     311
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII   AflIII     ApaI    ApaLI     AseI     AvaI
     BanI    BanII     BbsI    BbvCI     BcgI    BciVI Bme1580I     BmrI
     BmtI     BpmI   Bpu10I     BsaI    BsaBI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrDI   Bsu36I     BtgI
    BtgZI     ClaI     DraI   DraIII     EaeI  Eco57MI    EcoNI EcoO109I
     FspI    Hin4I   HincII  HindIII     KpnI     MscI     MslI   MspA1I
     NheI     NspI     PciI    PflMI     PfoI    PpuMI    PshAI   PspOMI
    PvuII     SmlI     StyI    TaqII     TatI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApoI     AscI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BplI    BpuEI    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtsI    CspCI     DrdI
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
    FspAI    HaeII     HpaI     KasI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI
     PasI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XmaI     XmnI     ZraI
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