Restriction map for pF1KB3766
 (Linear) (Six-base) MAPSORT of: KIBB3766.seq  Check: 5173  from: 1  to: 1512



KIBB3766.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     686     845    1010    1455    1512 
   Size:        686     159     165     445      57

  Fragments arranged by size: 

                 686     445     165     159      57
 
AleI CACnn'nnGTG

Cuts at:      0    1257    1512 
   Size:       1257     255
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1442    1512 
   Size:       1442      70
 
ApoI r'AATT_y

Cuts at:      0    1498    1512 
   Size:       1498      14
 
AvrII C'CTAG_G

Cuts at:      0     610    1512 
   Size:        610     902
 
BanI G'GyrC_C

Cuts at:      0    1262    1512 
   Size:       1262     250
 
BanII G_rGCy'C

Cuts at:      0      37     833    1512 
   Size:         37     796     679

  Fragments arranged by size: 

                 796     679      37
 
BclI T'GATC_A

Cuts at:      0    1116    1512 
   Size:       1116     396
 
BglI GCCn_nnn'nGGC

Cuts at:      0      32    1512 
   Size:         32    1480
 
BmtI G_CTAG'C

Cuts at:      0     160    1512 
   Size:        160    1352
 
Bpu10I CC'TnA_GC

Cuts at:      0    1097    1476    1512 
   Size:       1097     379      36

  Fragments arranged by size: 

                1097     379      36
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     701    1512 
   Size:        701     811
 
BseYI C'CCAG_C

Cuts at:      0      30    1512 
   Size:         30    1482
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251    1512 
   Size:        251    1261
 
BsiHKAI G_wGCw'C

Cuts at:      0     395     833    1512 
   Size:        395     438     679

  Fragments arranged by size: 

                 679     438     395
 
BsmI GAATG_Cn'

Cuts at:      0    1026    1512 
   Size:       1026     486
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     395     833    1512 
   Size:         37     358     438     679

  Fragments arranged by size: 

                 679     438     358      37
 
BspHI T'CATG_A

Cuts at:      0     881    1512 
   Size:        881     631
 
BsrDI GCAATG_nn'

Cuts at:      0     899    1512 
   Size:        899     613
 
BsrFI r'CCGG_y

Cuts at:      0      26    1512 
   Size:         26    1486
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     468    1512 
   Size:        468    1044
 
BstEII G'GTnAC_C

Cuts at:      0     196    1512 
   Size:        196    1316
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      30    1512 
   Size:         30    1482
 
BstYI r'GATC_y

Cuts at:      0      66    1386    1512 
   Size:         66    1320     126

  Fragments arranged by size: 

                1320     126      66
 
BtsI GCAGTG_nn'

Cuts at:      0     311     939    1252    1264    1512 
   Size:        311     628     313      12     248

  Fragments arranged by size: 

                 628     313     311     248      12
 
DraI TTT'AAA

Cuts at:      0     384    1497    1512 
   Size:        384    1113      15

  Fragments arranged by size: 

                1113     384      15
 
EarI CTCTTCn'nnn_

Cuts at:      0     821    1512 
   Size:        821     691
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1496    1512 
   Size:       1496      16
 
EcoICRI GAG'CTC

Cuts at:      0     831    1512 
   Size:        831     681
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     686     845    1010    1455    1512 
   Size:        686     159     165     445      57

  Fragments arranged by size: 

                 686     445     165     159      57
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     286     324     376    1512 
   Size:        286      38      52    1136

  Fragments arranged by size: 

                1136     286      52      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     154     186    1512 
   Size:        154      32    1326

  Fragments arranged by size: 

                1326     154      32
 
MfeI C'AATT_G

Cuts at:      0     295    1512 
   Size:        295    1217
 
MslI CAynn'nnrTG

Cuts at:      0    1257    1512 
   Size:       1257     255
 
MspA1I CmG'CkG

Cuts at:      0     148     823    1330    1512 
   Size:        148     675     507     182

  Fragments arranged by size: 

                 675     507     182     148
 
NaeI GCC'GGC

Cuts at:      0      28    1512 
   Size:         28    1484
 
NgoMIV G'CCGG_C

Cuts at:      0      26    1512 
   Size:         26    1486
 
NheI G'CTAG_C

Cuts at:      0     156    1512 
   Size:        156    1356
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     707     739    1297    1329    1512 
   Size:        707      32     558      32     183

  Fragments arranged by size: 

                 707     558     183      32      32
 
PsiI TTA'TAA

Cuts at:      0     445     595     677    1187    1512 
   Size:        445     150      82     510     325

  Fragments arranged by size: 

                 510     445     325     150      82
 
PstI C_TGCA'G

Cuts at:      0      74     318    1259    1512 
   Size:         74     244     941     253

  Fragments arranged by size: 

                 941     253     244      74
 
PvuII CAG'CTG

Cuts at:      0     148     823    1330    1512 
   Size:        148     675     507     182

  Fragments arranged by size: 

                 675     507     182     148
 
SacI G_AGCT'C

Cuts at:      0     833    1512 
   Size:        833     679
 
SapI GCTCTTCn'nnn_

Cuts at:      0     821    1512 
   Size:        821     691
 
SfcI C'TryA_G

Cuts at:      0      70     314     518    1255    1512 
   Size:         70     244     204     737     257

  Fragments arranged by size: 

                 737     257     244     204      70
 
StuI AGG'CCT

Cuts at:      0    1045    1512 
   Size:       1045     467
 
StyI C'CwwG_G

Cuts at:      0     610    1512 
   Size:        610     902
 
SwaI ATTT'AAAT

Cuts at:      0    1497    1512 
   Size:       1497      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1280    1512 
   Size:       1280     232
 
TatI w'GTAC_w

Cuts at:      0     692    1512 
   Size:        692     820
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     703     735    1512 
   Size:        703      32     777

  Fragments arranged by size: 

                 777     703      32
 
XbaI T'CTAG_A

Cuts at:      0    1137    1512 
   Size:       1137     375
 
XmnI GAAnn'nnTTC

Cuts at:      0    1076    1312    1512 
   Size:       1076     236     200

  Fragments arranged by size: 

                1076     236     200
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI    AvrII     BanI    BanII     BclI
     BglI     BmtI   Bpu10I     BsaI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI
     BtsI     DraI     EarI     EciI  EcoICRI  Eco57MI     FalI    Hin4I
     MfeI     MslI   MspA1I     NaeI   NgoMIV     NheI     PpiI     PsiI
     PstI    PvuII     SacI     SapI     SfcI     StuI     StyI     SwaI
    TaqII     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrGI   BssHII
    BssSI    BstBI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     NarI     NcoI     NdeI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI  Tth111I     XcmI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com