Restriction map for pF1KB3761
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        Alw44I      AocI        ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BseAI       BsiWI       BsmBI       
BsoBI       BspEI       BspHI       BspMI       BssHII      
Bst1107I    BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       CpoI        DrdI        Eco81I      
EcoO65I     EcoT22I     FbaI        FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MroI        MspA1I      
MunI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          393  GTCTAC               GT^[AC][GT]AC
AhdI         1308  GACTTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      2998  AGCGCT               AGC^GCT
AseI         2271  ATTAAT               AT^TAAT
AsnI         2271  ATTAAT               AT^TAAT
AspEI        1308  GACTTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         2114  TGGCCA               TGG^CCA
BanII        1636  GGGCTC               G[GA]GC[CT]^C
BbvCI          22  GCTGAGG              CCTCAGC(-5/-2)
BcnI           50  CCGGG                CC^[GC]GG
BglI           49  GCCGGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI        1114  ACCGGA               [AT]^CCGG[AT]
BsiEI         104  CGGCCG               CG[GA][CT]^CG
BsmI          859  GAATGC               GAATGC(1/-1)
Bsp1407I     3008  TGTACA               T^GTACA
BspDI        3103  ATCGAT               AT^CGAT
BsrBI        1623  CCGCTC               CCGCTC(-3/-3)
BsrDI         744  CATTGC               GCAATG(2/0)
BsrFI        1184  GCCGGT               [GA]^CCGG[CT]
BsrGI        3008  TGTACA               T^GTACA
BssSI        1010  CTCGTG               CACGAG(-5/-1)
Cfr10I       1184  GCCGGT               [GA]^CCGG[CT]
ClaI         3103  ATCGAT               AT^CGAT
DraI          679  TTTAAA               TTT^AAA
DraIII       1220  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          218  CCACGG               C^C[GA][CT]GG
EagI          104  CGGCCG               C^GGCCG
Eam1105I     1308  GACTTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         104  CGGCCG               C^GGCCG
Eco47III     2998  AGCGCT               AGC^GCT
Eco52I        104  CGGCCG               C^GGCCG
EcoRI        2473  GAATTC               G^AATTC
EcoRV        3046  GATATC               GAT^ATC
EcoT14I      1558  CCTTGG               C^C[AT][AT]GG
FauI         2964  CCCGC                CCCGC(4/6)
HincII       1316  GTCAAC               GT[CT]^[GA]AC
HindII       1316  GTCAAC               GT[CT]^[GA]AC
MluI         3132  ACGCGT               A^CGCGT
MluNI        2114  TGGCCA               TGG^CCA
MscI         2114  TGGCCA               TGG^CCA
MslI         2968  CACAAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI           50  CCGGG                CC^[GC]GG
PshBI        2271  ATTAAT               AT^TAAT
SapI          727  GAAGAGC              GCTCTTC(1/4)
SexAI        2091  ACCTGGT              A^CC[AT]GGT
SmlI         1641  CTCAAG               C^T[CT][GA]AG
SphI          196  GCATGC               GCATG^C
SspBI        3008  TGTACA               T^GTACA
StuI         2982  AGGCCT               AGG^CCT
StyI         1558  CCTTGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII         102  CGCG                 CG^CG
             3133  CGCG                
AlwNI          64  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
               76  CAGGAGCTG           
AspHI         802  GAGCAC               G[AT]GC[AT]^C
             1525  GTGCTC              
BcgI          120  CGACTTTACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3105  CGATAGTGATGC        
BpmI         1732  CTCCAG               CTGGAG(16/14)
             2186  CTGGAG              
BsaAI        2408  TACGTG               [CT]AC^GT[GA]
             2629  CACGTA              
BsgI         1715  CTGCAC               GTGCAG(16/14)
             1813  GTGCAG              
BsiHKAI       802  GAGCAC               G[AT]GC[AT]^C
             1525  GTGCTC              
BsmFI        1646  GGGAC                GGGAC(10/14)
             1818  GGGAC               
BspLU11I      329  ACATGT               A^CATGT
             1460  ACATGT              
BstAPI       1957  GCAGGGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2176  GCAAATGATGC         
BstBI        1874  TTCGAA               TT^CGAA
             1895  TTCGAA              
BstUI         102  CGCG                 CG^CG
             3133  CGCG                
Cac8I         196  GCATGC               GC[ACGT]^[ACGT]GC
             1386  GCTGGC              
EaeI          104  CGGCCG               [CT]^GGCC[GA]
             2114  TGGCCA              
HaeII          57  GGCGCT               [GA]GCGC^[CT]
             2998  AGCGCT              
HgaI          973  GACGC                GACGC(5/10)
             3134  GCGTC               
MvnI          102  CGCG                 CG^CG
             3133  CGCG                
NdeI          449  CATATG               CA^TATG
             2866  CATATG              
NlaIV        1989  GGACCC               GG[ACGT]^[ACGT]CC
             2356  GGGTCC              
NspV         1874  TTCGAA               TT^CGAA
             1895  TTCGAA              
PflMI        2161  CCATTACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2865  CCATATGATGG         
PpuMI        1988  AGGACCC              [GA]G^G[AT]CC[CT]
             2356  GGGTCCT             
SfuI         1874  TTCGAA               TT^CGAA
             1895  TTCGAA              
SspI          402  AATATT               AAT^ATT
             1240  AATATT              
Van91I       2161  CCATTACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2865  CCATATGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AvaII       BbsI        BbvI        
BfaI        BmyI        BpuAI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       CfoI        
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoNI       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspI        MvaI        MwoI        
NdeII       NlaIII      NspI        PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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