Restriction map for pF1KB3761
 (Linear) (Six-base) MAPSORT of: KIBB3761.seq  Check: 3426  from: 1  to: 3204



KIBB3761.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AccI GT'mk_AC

Cuts at:      0     394    3204 
   Size:        394    2810
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746    3204 
   Size:        746    2458
 
AfeI AGC'GCT

Cuts at:      0    3000    3204 
   Size:       3000     204
 
AflIII A'CryG_T

Cuts at:      0     329    1460    2464    3132    3204 
   Size:        329    1131    1004     668      72

  Fragments arranged by size: 

                1131    1004     668     329      72
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1313    3204 
   Size:       1313    1891
 
AlwNI CAG_nnn'CTG

Cuts at:      0      69      81    3204 
   Size:         69      12    3123

  Fragments arranged by size: 

                3123      69      12
 
ApoI r'AATT_y

Cuts at:      0     349     381    1394    2473    2794    3204 
   Size:        349      32    1013    1079     321     410

  Fragments arranged by size: 

                1079    1013     410     349     321      32
 
AseI AT'TA_AT

Cuts at:      0    2272    3204 
   Size:       2272     932
 
BanII G_rGCy'C

Cuts at:      0    1640    3204 
   Size:       1640    1564
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1449    1885    1980    3204 
   Size:       1449     436      95    1224

  Fragments arranged by size: 

                1449    1224     436      95
 
BbvCI CC'TCA_GC

Cuts at:      0      23    3204 
   Size:         23    3181
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     109     143    3094    3128    3204 
   Size:        109      34    2951      34      76

  Fragments arranged by size: 

                2951     109      76      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      55    3204 
   Size:         55    3149
 
Bme1580I G_kGCm'C

Cuts at:      0     649    3204 
   Size:        649    2555
 
BmgBI CAC'GTC

Cuts at:      0    2465    3204 
   Size:       2465     739
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2119    2363    3204 
   Size:       2119     244     841

  Fragments arranged by size: 

                2119     841     244
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     880    1656    3204 
   Size:        880     776    1548

  Fragments arranged by size: 

                1548     880     776
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1717    2207    3204 
   Size:       1717     490     997

  Fragments arranged by size: 

                1717     997     490
 
Bpu10I CC'TnA_GC

Cuts at:      0      23    2361    3204 
   Size:         23    2338     843

  Fragments arranged by size: 

                2338     843      23
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1626    3204 
   Size:       1626    1578
 
BsaAI yAC'GTr

Cuts at:      0    2410    2631    3204 
   Size:       2410     221     573

  Fragments arranged by size: 

                2410     573     221
 
BsaWI w'CCGG_w

Cuts at:      0    1114    3204 
   Size:       1114    2090
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1796    1826    3204 
   Size:       1796      30    1378

  Fragments arranged by size: 

                1796    1378      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     504    1835    2518    3204 
   Size:        504    1331     683     686

  Fragments arranged by size: 

                1331     686     683     504
 
BseYI C'CCAG_C

Cuts at:      0    2939    3204 
   Size:       2939     265
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1700    1834    3204 
   Size:       1700     134    1370

  Fragments arranged by size: 

                1700    1370     134
 
BsiEI CG_ry'CG

Cuts at:      0     107    3204 
   Size:        107    3097
 
BsiHKAI G_wGCw'C

Cuts at:      0     806    1529    3204 
   Size:        806     723    1675

  Fragments arranged by size: 

                1675     806     723
 
BsmI GAATG_Cn'

Cuts at:      0     865    3204 
   Size:        865    2339
 
Bsp1286I G_dGCh'C

Cuts at:      0     649     806    1529    1640    3204 
   Size:        649     157     723     111    1564

  Fragments arranged by size: 

                1564     723     649     157     111
 
BsrBI CCG'CTC

Cuts at:      0    1625    3204 
   Size:       1625    1579
 
BsrDI GCAATG_nn'

Cuts at:      0     743    3204 
   Size:        743    2461
 
BsrFI r'CCGG_y

Cuts at:      0    1184    3204 
   Size:       1184    2020
 
BsrGI T'GTAC_A

Cuts at:      0    3008    3204 
   Size:       3008     196
 
BssSI C'ACGA_G

Cuts at:      0    1010    3204 
   Size:       1010    2194
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1963    2182    3204 
   Size:       1963     219    1022

  Fragments arranged by size: 

                1963    1022     219
 
BstBI TT'CG_AA

Cuts at:      0    1875    1896    3204 
   Size:       1875      21    1308

  Fragments arranged by size: 

                1875    1308      21
 
BstYI r'GATC_y

Cuts at:      0     513     618     956    1351    3204 
   Size:        513     105     338     395    1853

  Fragments arranged by size: 

                1853     513     395     338     105
 
BtgI C'CryG_G

Cuts at:      0     218    3204 
   Size:        218    2986
 
BtsI GCAGTG_nn'

Cuts at:      0     510     908    3204 
   Size:        510     398    2296

  Fragments arranged by size: 

                2296     510     398
 
ClaI AT'CG_AT

Cuts at:      0    3104    3204 
   Size:       3104     100
 
DraI TTT'AAA

Cuts at:      0     681    3204 
   Size:        681    2523
 
DraIII CAC_nnn'GTG

Cuts at:      0    1225    3204 
   Size:       1225    1979
 
EaeI y'GGCC_r

Cuts at:      0     104    2114    3204 
   Size:        104    2010    1090

  Fragments arranged by size: 

                2010    1090     104
 
EagI C'GGCC_G

Cuts at:      0     104    3204 
   Size:        104    3100
 
EarI CTCTTCn'nnn_

Cuts at:      0     722     914     974    1625    1893    2108    2814    3204 
   Size:        722     192      60     651     268     215     706     390

  Fragments arranged by size: 

                 722     706     651     390     268     215     192      60
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746    1717    2207    3204 
   Size:        746     971     490     997

  Fragments arranged by size: 

                 997     971     746     490
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      73      91    1955    3204 
   Size:         73      18    1864    1249

  Fragments arranged by size: 

                1864    1249      73      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66    1989    2357    3204 
   Size:         66    1923     368     847

  Fragments arranged by size: 

                1923     847     368      66
 
EcoRI G'AATT_C

Cuts at:      0    2473    3204 
   Size:       2473     731
 
EcoRV GAT'ATC

Cuts at:      0    3048    3204 
   Size:       3048     156
 
HaeII r_GCGC'y

Cuts at:      0      61    3002    3204 
   Size:         61    2941     202

  Fragments arranged by size: 

                2941     202      61
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1299    1331    2862    2894    3103    3135    3204 
   Size:       1299      32    1531      32     209      32      69

  Fragments arranged by size: 

                1531    1299     209      69      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1318    3204 
   Size:       1318    1886
 
MluI A'CGCG_T

Cuts at:      0    3132    3204 
   Size:       3132      72
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     511    3204 
   Size:        204     307    2693

  Fragments arranged by size: 

                2693     307     204
 
MscI TGG'CCA

Cuts at:      0    2116    3204 
   Size:       2116    1088
 
MslI CAynn'nnrTG

Cuts at:      0    2972    3204 
   Size:       2972     232
 
NdeI CA'TA_TG

Cuts at:      0     450    2867    3204 
   Size:        450    2417     337

  Fragments arranged by size: 

                2417     450     337
 
NspI r_CATG'y

Cuts at:      0     182     200     333    1464    3204 
   Size:        182      18     133    1131    1740

  Fragments arranged by size: 

                1740    1131     182     133      18
 
PciI A'CATG_T

Cuts at:      0     329    1460    3204 
   Size:        329    1131    1744

  Fragments arranged by size: 

                1744    1131     329
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2167    2871    3204 
   Size:       2167     704     333

  Fragments arranged by size: 

                2167     704     333
 
PfoI T'CCnGG_A

Cuts at:      0     866    3204 
   Size:        866    2338
 
PpuMI rG'GwC_Cy

Cuts at:      0    1989    2357    3204 
   Size:       1989     368     847

  Fragments arranged by size: 

                1989     847     368
 
PsiI TTA'TAA

Cuts at:      0    1237    1406    1601    2791    3204 
   Size:       1237     169     195    1190     413

  Fragments arranged by size: 

                1237    1190     413     195     169
 
SapI GCTCTTCn'nnn_

Cuts at:      0     722    3204 
   Size:        722    2482
 
SexAI A'CCwGG_T

Cuts at:      0    2091    3204 
   Size:       2091    1113
 
SfcI C'TryA_G

Cuts at:      0      61     144    1536    1941    3172    3204 
   Size:         61      83    1392     405    1231      32

  Fragments arranged by size: 

                1392    1231     405      83      61      32
 
SmlI C'TyrA_G

Cuts at:      0    1641    3204 
   Size:       1641    1563
 
SphI G_CATG'C

Cuts at:      0     200    3204 
   Size:        200    3004
 
SspI AAT'ATT

Cuts at:      0     404    1242    3204 
   Size:        404     838    1962

  Fragments arranged by size: 

                1962     838     404
 
StuI AGG'CCT

Cuts at:      0    2984    3204 
   Size:       2984     220
 
StyI C'CwwG_G

Cuts at:      0    1558    3204 
   Size:       1558    1646
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1186    1871    3204 
   Size:       1186     685    1333

  Fragments arranged by size: 

                1333    1186     685
 
TatI w'GTAC_w

Cuts at:      0     750    3008    3204 
   Size:        750    2258     196

  Fragments arranged by size: 

                2258     750     196
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1796    1828    3204 
   Size:       1796      32    1376

  Fragments arranged by size: 

                1796    1376      32
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AhdI    AlwNI     ApoI     AseI
    BanII     BbsI    BbvCI     BcgI     BglI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI     BtsI
     ClaI     DraI   DraIII     EaeI     EagI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII     MluI     MmeI
     MscI     MslI     NdeI     NspI     PciI    PflMI     PfoI    PpuMI
     PsiI     SapI    SexAI     SfcI     SmlI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI    BciVI     BclI    BfrBI    BglII     BlpI
     BmtI     BsaI    BsaBI    BsaHI    BsiWI    BsmBI    BspEI    BspHI
    BspMI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI    CspCI
     DrdI     EciI  EcoICRI     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com