Restriction map for
pF1KB3732
(Circular) (Six-base) MAPSORT of: pF1KB3732.seq Check: 9075 from: 1 to: 4496
pF1KB3732 4496 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 897 1210 1232 2321 2753 3106 3768 227
Size: 670 313 22 1089 432 353 662 955
Fragments arranged by size:
1089 955 670 662 432 353 313 22
AflIII A'CryG_T
Cuts at: 475 1848 3648 3929 475
Size: 1373 1800 281 1042
Fragments arranged by size:
1800 1373 1042 281
AlwNI CAG_nnn'CTG
Cuts at: 821 1801 3239 821
Size: 980 1438 2078
Fragments arranged by size:
2078 1438 980
ApaLI G'TGCA_C
Cuts at: 3334 3334
Size: 4496
ApoI r'AATT_y
Cuts at: 838 1500 3849 4105 838
Size: 662 2349 256 1229
Fragments arranged by size:
2349 1229 662 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 1257 1516 1257
Size: 259 4237
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 453 486 453
Size: 33 4463
BamHI G'GATC_C
Cuts at: 1521 1521
Size: 4496
BanI G'GyrC_C
Cuts at: 1512 1926 2174 2209 1512
Size: 414 248 35 3799
Fragments arranged by size:
3799 414 248 35
BanII G_rGCy'C
Cuts at: 425 749 770 1510 2540 425
Size: 324 21 740 1030 2381
Fragments arranged by size:
2381 1030 740 324 21
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 857 1010 1181 1238 857
Size: 153 171 57 4115
Fragments arranged by size:
4115 171 153 57
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 569 603 569
Size: 34 4462
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3450 4496 2394
Size: 1056 1046 2394
Fragments arranged by size:
2394 1056 1046
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BfrBI ATG'CAT
Cuts at: 614 614
Size: 4496
BglI GCCn_nnn'nGGC
Cuts at: 796 796
Size: 4496
BglII A'GATC_T
Cuts at: 1386 2011 4048 1386
Size: 625 2037 1834
Fragments arranged by size:
2037 1834 625
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 357 737 2121 2214 3338 357
Size: 380 1384 93 1124 1515
Fragments arranged by size:
1515 1384 1124 380 93
BmrI ACTGGGnnnn_n'
Cuts at: 395 1708 1789 2124 4268 395
Size: 1313 81 335 2144 623
Fragments arranged by size:
2144 1313 623 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 1672 2551 3265 3563 333
Size: 1339 879 714 298 1266
Fragments arranged by size:
1339 1266 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 476 1347 1849 476
Size: 871 502 3123
Fragments arranged by size:
3123 871 502
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 1160 1927 2175 2877 4373 1160
Size: 767 248 702 1496 1283
Fragments arranged by size:
1496 1283 767 702 248
BsaWI w'CCGG_w
Cuts at: 1685 2206 2884 3295 3442 3716 1685
Size: 521 678 411 147 274 2465
Fragments arranged by size:
2465 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1030 1060 1030
Size: 30 4466
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 700 1009 388
Size: 312 309 3875
Fragments arranged by size:
3875 312 309
BseYI C'CCAG_C
Cuts at: 1921 2679 3344 3752 1921
Size: 758 665 408 2665
Fragments arranged by size:
2665 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 1411
Size: 4496
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3314 104
Size: 1934 46 1230 1286
Fragments arranged by size:
1934 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 425 770 1510 2288 2478 3338 425
Size: 345 740 778 190 860 1583
Fragments arranged by size:
1583 860 778 740 345 190
BsmI GAATG_Cn'
Cuts at: 1447 1447
Size: 4496
Bsp1286I G_dGCh'C
Cuts at: 357 425 737 749 770 1510 2121 2214 2288
Size: 68 312 12 21 740 611 93 74
Cuts at: 2288 2478 2540 3338 357
Size: 190 62 798 1515
Fragments arranged by size:
1515 798 740 611 312 190 93 74
68 62 21 12
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1546 1760 2062 2443 1546
Size: 214 302 381 3599
Fragments arranged by size:
3599 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2408 2408
Size: 4496
BsrFI r'CCGG_y
Cuts at: 2494 2675 2494
Size: 181 4315
BssSI C'ACGA_G
Cuts at: 2767 3475 2767
Size: 708 3788
BstAPI GCAn_nnn'nTGC
Cuts at: 1873 1873
Size: 4496
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstXI CCAn_nnnn'nTGG
Cuts at: 957 957
Size: 4496
BstYI r'GATC_y
Cuts at: 1 1386 1521 1988 2011 2346 2592 2898 2910
Size: 1385 135 467 23 335 246 306 12
Cuts at: 2910 2996 4048 1
Size: 86 1052 449
Fragments arranged by size:
1385 1052 467 449 335 306 246 135
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2446 2627 2790 2446
Size: 181 163 4152
Fragments arranged by size:
4152 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 1739 1118
Size: 621 3875
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DraIII CAC_nnn'GTG
Cuts at: 1347 1347
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 343 2202 3546 343
Size: 1859 1344 1293
Fragments arranged by size:
1859 1344 1293
EaeI y'GGCC_r
Cuts at: 665 1360 2081 2255 2646 2673 4018 665
Size: 695 721 174 391 27 1345 1143
Fragments arranged by size:
1345 1143 721 695 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 220 707 1462 2519 2729 220
Size: 487 755 1057 210 1987
Fragments arranged by size:
1987 1057 755 487 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3446 3592 4306 2704
Size: 742 146 714 2894
Fragments arranged by size:
2894 742 714 146
EcoICRI GAG'CTC
Cuts at: 423 768 1508 423
Size: 345 740 3411
Fragments arranged by size:
3411 740 345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 897 1210 1232 2321 2753 3106 3768 227
Size: 670 313 22 1089 432 353 662 955
Fragments arranged by size:
1089 955 670 662 432 353 313 22
EcoNI CCTnn'n_nnAGG
Cuts at: 1225 1375 1225
Size: 150 4346
EcoO109I rG'GnC_Cy
Cuts at: 1635 1635
Size: 4496
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 513 918 1103 2515 513
Size: 405 185 1412 2494
Fragments arranged by size:
2494 1412 405 185
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 1930 2178 3408 4179 1930
Size: 248 1230 771 2247
Fragments arranged by size:
2247 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1030 1062 2338 2368 2370 2400 1030
Size: 32 1276 30 2 30 3126
Fragments arranged by size:
3126 1276 32 30 30 2
HincII GTy'rAC
Cuts at: 900 1535 900
Size: 635 3861
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3255 3439 3255
Size: 184 4312
MscI TGG'CCA
Cuts at: 667 1362 2257 667
Size: 695 895 2906
Fragments arranged by size:
2906 895 695
MslI CAynn'nnrTG
Cuts at: 326 1435 2485 2612 326
Size: 1109 1050 127 2210
Fragments arranged by size:
2210 1109 1050 127
MspA1I CmG'CkG
Cuts at: 467 1128 1607 1921 2281 3065 3310 467
Size: 661 479 314 360 784 245 1653
Fragments arranged by size:
1653 784 661 479 360 314 245
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NsiI A_TGCA'T
Cuts at: 616 616
Size: 4496
NspI r_CATG'y
Cuts at: 1182 1549 2049 3652 1182
Size: 367 500 1603 2026
Fragments arranged by size:
2026 1603 500 367
PciI A'CATG_T
Cuts at: 3648 3648
Size: 4496
PflMI CCAn_nnn'nTGG
Cuts at: 889 889
Size: 4496
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PmlI CAC'GTG
Cuts at: 476 1347 476
Size: 871 3625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2942 2974 2942
Size: 32 4464
PstI C_TGCA'G
Cuts at: 722 1543 2228 722
Size: 821 685 2990
Fragments arranged by size:
2990 821 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1128 1921 2281 1128
Size: 793 360 3343
Fragments arranged by size:
3343 793 360
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 425 770 1510 425
Size: 345 740 3411
Fragments arranged by size:
3411 740 345
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 220 707 2519 2729 220
Size: 487 1812 210 1987
Fragments arranged by size:
1987 1812 487 210
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 718 1539 2224 3192 3383 33
Size: 685 821 685 968 191 1146
Fragments arranged by size:
1146 968 821 685 685 191
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1259 1518 1259
Size: 259 4237
SmlI C'TyrA_G
Cuts at: 348 1651 2566 3280 3542 348
Size: 1303 915 714 262 1302
Fragments arranged by size:
1303 1302 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
SspI AAT'ATT
Cuts at: 308 308
Size: 4496
StyI C'CwwG_G
Cuts at: 810 1630 1984 810
Size: 820 354 3322
Fragments arranged by size:
3322 820 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 188 2079 2212 2671 2880 188
Size: 1891 133 459 209 1804
Fragments arranged by size:
1891 1804 459 209 133
TatI w'GTAC_w
Cuts at: 4011 4011
Size: 4496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 266 298 4013 4045 266
Size: 32 3715 32 717
Fragments arranged by size:
3715 717 32 32
Tth111I GACn'n_nGTC
Cuts at: 571 2293 571
Size: 1722 2774
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1132 1170 1991 1132
Size: 38 821 3637
Fragments arranged by size:
3637 821 38
XmaI C'CCGG_G
Cuts at: 1257 1516 1257
Size: 259 4237
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PflMI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BbvCI BmgBI BmtI BpmI
Bpu10I BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI