Restriction map for pF1KB3732
 (Linear) (Six-base) MAPSORT of: KIBB3732.seq  Check: 1659  from: 1  to: 1383



KIBB3732.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     789    1102    1124    1383 
   Size:        119     670     313      22     259

  Fragments arranged by size: 

                 670     313     259     119      22
 
AflIII A'CryG_T

Cuts at:      0     367    1383 
   Size:        367    1016
 
AlwNI CAG_nnn'CTG

Cuts at:      0     713    1383 
   Size:        713     670
 
ApoI r'AATT_y

Cuts at:      0     730    1383 
   Size:        730     653
 
AvaI C'yCGr_G

Cuts at:      0    1149    1383 
   Size:       1149     234
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     345     378    1383 
   Size:        345      33    1005

  Fragments arranged by size: 

                1005     345      33
 
BanII G_rGCy'C

Cuts at:      0     317     641     662    1383 
   Size:        317     324      21     721

  Fragments arranged by size: 

                 721     324     317      21
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     749     902    1073    1130    1383 
   Size:        749     153     171      57     253

  Fragments arranged by size: 

                 749     253     171     153      57
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     461     495    1383 
   Size:        461      34     888

  Fragments arranged by size: 

                 888     461      34
 
BfrBI ATG'CAT

Cuts at:      0     506    1383 
   Size:        506     877
 
BglI GCCn_nnn'nGGC

Cuts at:      0     688    1383 
   Size:        688     695
 
BglII A'GATC_T

Cuts at:      0    1278    1383 
   Size:       1278     105
 
Bme1580I G_kGCm'C

Cuts at:      0     249     629    1383 
   Size:        249     380     754

  Fragments arranged by size: 

                 754     380     249
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     287    1383 
   Size:        287    1096
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225    1383 
   Size:        225    1158
 
BsaAI yAC'GTr

Cuts at:      0     368    1239    1383 
   Size:        368     871     144

  Fragments arranged by size: 

                 871     368     144
 
BsaHI Gr'CG_yC

Cuts at:      0    1052    1383 
   Size:       1052     331
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     922     952    1383 
   Size:        922      30     431

  Fragments arranged by size: 

                 922     431      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     280     592     901    1383 
   Size:        280     312     309     482

  Fragments arranged by size: 

                 482     312     309     280
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1303    1383 
   Size:       1303      80
 
BsiHKAI G_wGCw'C

Cuts at:      0     317     662    1383 
   Size:        317     345     721

  Fragments arranged by size: 

                 721     345     317
 
BsmI GAATG_Cn'

Cuts at:      0    1339    1383 
   Size:       1339      44
 
Bsp1286I G_dGCh'C

Cuts at:      0     249     317     629     641     662    1383 
   Size:        249      68     312      12      21     721

  Fragments arranged by size: 

                 721     312     249      68      21      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     849    1383 
   Size:        849     534
 
BstYI r'GATC_y

Cuts at:      0    1278    1383 
   Size:       1278     105
 
BtsI GCAGTG_nn'

Cuts at:      0    1010    1383 
   Size:       1010     373
 
DraIII CAC_nnn'GTG

Cuts at:      0    1239    1383 
   Size:       1239     144
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     235    1383 
   Size:        235    1148
 
EaeI y'GGCC_r

Cuts at:      0     557    1252    1383 
   Size:        557     695     131

  Fragments arranged by size: 

                 695     557     131
 
EarI CTCTTCn'nnn_

Cuts at:      0     112     599    1354    1383 
   Size:        112     487     755      29

  Fragments arranged by size: 

                 755     487     112      29
 
EcoICRI GAG'CTC

Cuts at:      0     315     660    1383 
   Size:        315     345     723

  Fragments arranged by size: 

                 723     345     315
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     789    1102    1124    1383 
   Size:        119     670     313      22     259

  Fragments arranged by size: 

                 670     313     259     119      22
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1117    1267    1383 
   Size:       1117     150     116

  Fragments arranged by size: 

                1117     150     116
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     405     810     995    1383 
   Size:        405     405     185     388

  Fragments arranged by size: 

                 405     405     388     185
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     922     954    1383 
   Size:        922      32     429

  Fragments arranged by size: 

                 922     429      32
 
HincII GTy'rAC

Cuts at:      0     792    1383 
   Size:        792     591
 
MscI TGG'CCA

Cuts at:      0     559    1254    1383 
   Size:        559     695     129

  Fragments arranged by size: 

                 695     559     129
 
MslI CAynn'nnrTG

Cuts at:      0     218    1327    1383 
   Size:        218    1109      56

  Fragments arranged by size: 

                1109     218      56
 
MspA1I CmG'CkG

Cuts at:      0     359    1020    1383 
   Size:        359     661     363

  Fragments arranged by size: 

                 661     363     359
 
NsiI A_TGCA'T

Cuts at:      0     508    1383 
   Size:        508     875
 
NspI r_CATG'y

Cuts at:      0    1074    1383 
   Size:       1074     309
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     781    1383 
   Size:        781     602
 
PmlI CAC'GTG

Cuts at:      0     368    1239    1383 
   Size:        368     871     144

  Fragments arranged by size: 

                 871     368     144
 
PstI C_TGCA'G

Cuts at:      0     614    1383 
   Size:        614     769
 
PvuII CAG'CTG

Cuts at:      0    1020    1383 
   Size:       1020     363
 
SacI G_AGCT'C

Cuts at:      0     317     662    1383 
   Size:        317     345     721

  Fragments arranged by size: 

                 721     345     317
 
SapI GCTCTTCn'nnn_

Cuts at:      0     112     599    1383 
   Size:        112     487     784

  Fragments arranged by size: 

                 784     487     112
 
SfcI C'TryA_G

Cuts at:      0     610    1383 
   Size:        610     773
 
SmaI CCC'GGG

Cuts at:      0    1151    1383 
   Size:       1151     232
 
SmlI C'TyrA_G

Cuts at:      0     240    1383 
   Size:        240    1143
 
SspI AAT'ATT

Cuts at:      0     200    1383 
   Size:        200    1183
 
StyI C'CwwG_G

Cuts at:      0     702    1383 
   Size:        702     681
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      80    1383 
   Size:         80    1303
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     158     190    1383 
   Size:        158      32    1193

  Fragments arranged by size: 

                1193     158      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     463    1383 
   Size:        463     920
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1024    1062    1383 
   Size:       1024      38     321

  Fragments arranged by size: 

                1024     321      38
 
XmaI C'CCGG_G

Cuts at:      0    1149    1383 
   Size:       1149     234
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     AvaI     BaeI    BanII     BbsI
     BcgI    BfrBI     BglI    BglII Bme1580I     BmrI    BpuEI    BsaAI
    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BstXI
    BstYI     BtsI   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI     FalI    Hin4I   HincII     MscI     MslI   MspA1I     NsiI
     NspI    PflMI     PmlI     PstI    PvuII     SacI     SapI     SfcI
     SmaI     SmlI     SspI     StyI    TaqII     TstI  Tth111I     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII    BamHI     BanI     BbeI    BbvCI    BciVI     BclI
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI
    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
     EagI     EciI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI     XbaI
     XhoI     XmnI     ZraI
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