Restriction map for pF1KB3725
 (Circular) (Six-base) MAPSORT of: pF1KB3725.seq  Check: 4958  from: 1  to: 7238



pF1KB3725  7238 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AccI GT'mk_AC

Cuts at:    776    1902    2189    4276     776 
   Size:       1126     287    2087    3738

  Fragments arranged by size: 

                3738    2087    1126     287
 
Acc65I G'GTAC_C

Cuts at:   1634    2629    3012    4254    1634 
   Size:        995     383    1242    4618

  Fragments arranged by size: 

                4618    1242     995     383
 
AclI AA'CG_TT

Cuts at:   7078    7078 
   Size:       7238
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    712    1250    1639    2323    3233    3362    3982    4055    4127 
   Size:        538     389     684     910     129     620      73      72

Cuts at:   4127    5063    5495    6242    6510     712 
   Size:        936     432     747     268    1440

  Fragments arranged by size: 

                1440     936     910     747     684     620     538     432
                 389     268     129      73      72
 
AflII C'TTAA_G

Cuts at:   3967    3967 
   Size:       7238
 
AflIII A'CryG_T

Cuts at:   4144    4590    5694    6671    4144 
   Size:        446    1104     977    4711

  Fragments arranged by size: 

                4711    1104     977     446
 
AgeI A'CCGG_T

Cuts at:   3824    5626    3824 
   Size:       1802    5436
 
AhdI GACnn_n'nnGTC

Cuts at:   2700    2700 
   Size:       7238
 
AlwNI CAG_nnn'CTG

Cuts at:    269     690    2029    2271    2328    2875    4543    6110     269 
   Size:        421    1339     242      57     547    1668    1567    1397

  Fragments arranged by size: 

                1668    1567    1397    1339     547     421     242      57
            
 
ApaLI G'TGCA_C

Cuts at:    723    4188    6008     723 
   Size:       3465    1820    1953

  Fragments arranged by size: 

                3465    1953    1820
 
ApoI r'AATT_y

Cuts at:   1001    1914    1954    3146    3180    3397    3986    4014    4242 
   Size:        913      40    1192      34     217     589      28     228

Cuts at:   4242    6591    6847    1001 
   Size:       2349     256    1392

  Fragments arranged by size: 

                2349    1392    1192     913     589     256     228     217
                  40      34      28
 
AseI AT'TA_AT

Cuts at:     20    1406    1792    2540      20 
   Size:       1386     386     748    4718

  Fragments arranged by size: 

                4718    1386     748     386
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7238
 
AvaI C'yCGr_G

Cuts at:    162     181     324     338    4258     162 
   Size:         19     143      14    3920    3142

  Fragments arranged by size: 

                3920    3142     143      19      14
 
BamHI G'GATC_C

Cuts at:   3278    4263    3278 
   Size:        985    6253
 
BanI G'GyrC_C

Cuts at:    478    1634    2629    2646    3012    4254    4668    4916    4951 
   Size:       1156     995      17     366    1242     414     248      35

Cuts at:   4951     478 
   Size:       2765

  Fragments arranged by size: 

                2765    1242    1156     995     414     366     248      35
                  17
 
BanII G_rGCy'C

Cuts at:    818    1463    2335    4252    5282     818 
   Size:        645     872    1917    1030    2774

  Fragments arranged by size: 

                2774    1917    1030     872     645
 
BbeI G_GCGC'C

Cuts at:    482    4672    4920     482 
   Size:       4190     248    2800

  Fragments arranged by size: 

                4190    2800     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1483    1627    3296    3686    3741    4088    1483 
   Size:        144    1669     390      55     347    4633

  Fragments arranged by size: 

                4633    1669     390     347     144      55
 
BbvCI CC'TCA_GC

Cuts at:   3241    3241 
   Size:       7238
 
BciVI GTATCCnnnnn_n'

Cuts at:   5136    5897    7238    5136 
   Size:        761    1341    5136

  Fragments arranged by size: 

                5136    1341     761
 
BclI T'GATC_A

Cuts at:    395    3672    4758     395 
   Size:       3277    1086    2875

  Fragments arranged by size: 

                3277    2875    1086
 
BfrBI ATG'CAT

Cuts at:   1174    1174 
   Size:       7238
 
BglII A'GATC_T

Cuts at:   2564    4753    6790    2564 
   Size:       2189    2037    3012

  Fragments arranged by size: 

                3012    2189    2037
 
BlpI GC'TnA_GC

Cuts at:   4350    4350 
   Size:       7238
 
Bme1580I G_kGCm'C

Cuts at:    727    4192    4863    4956    6012     727 
   Size:       3465     671      93    1056    1953

  Fragments arranged by size: 

                3465    1953    1056     671      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    737    1542    2767    4450    4531    4866    7010     737 
   Size:        805    1225    1683      81     335    2144     965

  Fragments arranged by size: 

                2144    1683    1225     965     805     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3072    5605    3072 
   Size:       2533    4705
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1507    2140    2402    2620    3475    1507 
   Size:        633     262     218     855    5270

  Fragments arranged by size: 

                5270     855     633     262     218
 
Bpu10I CC'TnA_GC

Cuts at:   2954    3241    3332    2954 
   Size:        287      91    6860

  Fragments arranged by size: 

                6860     287      91
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1624    2226    2545    4185    4414    5293    5785    6083    1624 
   Size:        602     319    1640     229     879     492     298    2779

  Fragments arranged by size: 

                2779    1640     879     602     492     319     298     229
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   1337    2708    6942    1337 
   Size:       1371    4234    1633

  Fragments arranged by size: 

                4234    1633    1371
 
BsaAI yAC'GTr

Cuts at:   1878    4147    4591    1878 
   Size:       2269     444    4525

  Fragments arranged by size: 

                4525    2269     444
 
BsaBI GATnn'nnATC

Cuts at:      6    4752    6789       6 
   Size:       4746    2037     455

  Fragments arranged by size: 

                4746    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    479    4669    4917    5619    7115     479 
   Size:       4190     248     702    1496     602

  Fragments arranged by size: 

                4190    1496     702     602     248
 
BsaWI w'CCGG_w

Cuts at:   1463    3824    4427    4948    5626    5900    6047    6458    1463 
   Size:       2361     603     521     678     274     147     411    2243

  Fragments arranged by size: 

                2361    2243     678     603     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   3899    3929    3899 
   Size:         30    7208
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    221     247     312    1411    3191     221 
   Size:         26      65    1099    1780    4268

  Fragments arranged by size: 

                4268    1780    1099      65      26
 
BseYI C'CCAG_C

Cuts at:   4663    5421    5998    6494    4663 
   Size:        758     577     496    5407

  Fragments arranged by size: 

                5407     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    156     156 
   Size:       7238
 
BsiEI CG_ry'CG

Cuts at:    104    4780    4826    6034     104 
   Size:       4676      46    1208    1308

  Fragments arranged by size: 

                4676    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    115     239     727     818    1463    3201    3784    3822    4192 
   Size:        124     488      91     645    1738     583      38     370

Cuts at:   4192    4252    5030    5220    6012     115 
   Size:         60     778     190     792    1341

  Fragments arranged by size: 

                1738    1341     792     778     645     583     488     370
                 190     124      91      60      38
 
BsmI GAATG_Cn'

Cuts at:   2845    3167    2845 
   Size:        322    6916
 
Bsp1286I G_dGCh'C

Cuts at:    115     239     727     818    1463    2335    3201    3784    3822 
   Size:        124     488      91     645     872     866     583      38

Cuts at:   3822    4192    4252    4863    4956    5030    5220    5282    6012 
   Size:        370      60     611      93      74     190      62     730

Cuts at:   6012     115 
   Size:       1341

  Fragments arranged by size: 

                1341     872     866     730     645     611     583     488
                 370     190     124      93      91      74      62      60
                  38
 
BspEI T'CCGG_A

Cuts at:   1463    6458    1463 
   Size:       4995    2243
 
BspHI T'CATG_A

Cuts at:    797    3804    6414     797 
   Size:       3007    2610    1621

  Fragments arranged by size: 

                3007    2610    1621
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2185    2364    4288    4502    4804    5185    2185 
   Size:        179    1924     214     302     381    4238

  Fragments arranged by size: 

                4238    1924     381     302     214     179
 
BsrBI CCG'CTC

Cuts at:     50     211    5530    5584    7068    7233      50 
   Size:        161    5319      54    1484     165      55

  Fragments arranged by size: 

                5319    1484     165     161      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2287    3380    5150    2287 
   Size:       1093    1770    4375

  Fragments arranged by size: 

                4375    1770    1093
 
BsrFI r'CCGG_y

Cuts at:   3009    3824    4094    5236    5417    5626    3009 
   Size:        815     270    1142     181     209    4621

  Fragments arranged by size: 

                4621    1142     815     270     209     181
 
BsrGI T'GTAC_A

Cuts at:   2020    2713    3837    2020 
   Size:        693    1124    5421

  Fragments arranged by size: 

                5421    1124     693
 
BssSI C'ACGA_G

Cuts at:   5509    5867    5509 
   Size:        358    6880
 
BstAPI GCAn_nnn'nTGC

Cuts at:    243     453     559    4615     243 
   Size:        210     106    4056    2866

  Fragments arranged by size: 

                4056    2866     210     106
 
BstBI TT'CG_AA

Cuts at:   4218    5599    4218 
   Size:       1381    5857
 
BstXI CCAn_nnnn'nTGG

Cuts at:    534     534 
   Size:       7238
 
BstYI r'GATC_y

Cuts at:      1    2564    2813    2871    3278    3406    4263    4730    4753 
   Size:       2563     249      58     407     128     857     467      23

Cuts at:   4753    5088    5334    6346    6432    6444    6790       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                2563    1012     857     467     449     407     346     335
                 249     246     128      86      58      23      12
 
BstZ17I GTA'TAC

Cuts at:   1903    1903 
   Size:       7238
 
Bsu36I CC'TnA_GG

Cuts at:   3740    3771    3740 
   Size:         31    7207
 
BtgI C'CryG_G

Cuts at:    380    1238    1347    3474     380 
   Size:        858     109    2127    4144

  Fragments arranged by size: 

                4144    2127     858     109
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    593    5188    5369    5532     593 
   Size:       4595     181     163    2299

  Fragments arranged by size: 

                4595    2299     181     163
 
BtsI GCAGTG_nn'

Cuts at:    336    3051    4481     336 
   Size:       2715    1430    3093

  Fragments arranged by size: 

                3093    2715    1430
 
DraI TTT'AAA

Cuts at:   1614    2799    2921    4237    1614 
   Size:       1185     122    1316    4615

  Fragments arranged by size: 

                4615    1316    1185     122
 
DraIII CAC_nnn'GTG

Cuts at:   1158    4196    1158 
   Size:       3038    4200
 
DrdI GACnn_nn'nnGTC

Cuts at:   4944    5802    4944 
   Size:        858    6380
 
EaeI y'GGCC_r

Cuts at:    314     428     638    1706    2729    4823    4997    5388    5415 
   Size:        114     210    1068    1023    2094     174     391      27

Cuts at:   5415    6760     314 
   Size:       1345     792

  Fragments arranged by size: 

                2094    1345    1068    1023     792     391     210     174
                 114      27
 
EagI C'GGCC_G

Cuts at:   4823    4823 
   Size:       7238
 
EarI CTCTTCn'nnn_

Cuts at:   1932    2066    5261    5471    1932 
   Size:        134    3195     210    3699

  Fragments arranged by size: 

                3699    3195     210     134
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    136    5446    5756    5902    7048     136 
   Size:       5310     310     146    1146     326

  Fragments arranged by size: 

                5310    1146     326     310     146
 
EcoICRI GAG'CTC

Cuts at:    816    1461    4250     816 
   Size:        645    2789    3804

  Fragments arranged by size: 

                3804    2789     645
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    712    1250    1507    1639    2140    2323    2402    2620    3233 
   Size:        538     257     132     501     183      79     218     613

Cuts at:   3233    3362    3475    3982    4055    4127    5063    5495    6242 
   Size:        129     113     507      73      72     936     432     747

Cuts at:   6242    6510     712 
   Size:        268    1440

  Fragments arranged by size: 

                1440     936     747     613     538     507     501     432
                 268     257     218     183     132     129     113      79
                  73      72
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1747    1747 
   Size:       7238
 
EcoO109I rG'GnC_Cy

Cuts at:    441    2376    4377     441 
   Size:       1935    2001    3302

  Fragments arranged by size: 

                3302    2001    1935
 
EcoRI G'AATT_C

Cuts at:   1001    3146    4242    1001 
   Size:       2145    1096    3997

  Fragments arranged by size: 

                3997    2145    1096
 
EcoRV GAT'ATC

Cuts at:   1077    1077 
   Size:       7238
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1527    3934    5257    1527 
   Size:       2407    1323    3508

  Fragments arranged by size: 

                3508    2407    1323
 
FspI TGC'GCA

Cuts at:   5019    5019 
   Size:       7238
 
HaeII r_GCGC'y

Cuts at:    287     482    4672    4920    5942    6921     287 
   Size:        195    4190     248    1022     979     604

  Fragments arranged by size: 

                4190    1022     979     604     248     195
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    655     687    1129    1161    2686    2718    2812    2844    3082 
   Size:         32     442      32    1525      32      94      32     238

Cuts at:   3082    3114    5080    5110    5112    5142     655 
   Size:         32    1966      30       2      30    2751

  Fragments arranged by size: 

                2751    1966    1525     442     238      94      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:    809    4277     809 
   Size:       3468    3770
 
HindIII A'AGCT_T

Cuts at:   1606    2956    3964    1606 
   Size:       1350    1008    4880

  Fragments arranged by size: 

                4880    1350    1008
 
KasI G'GCGC_C

Cuts at:    478    4668    4916     478 
   Size:       4190     248    2800

  Fragments arranged by size: 

                4190    2800     248
 
KpnI G_GTAC'C

Cuts at:   1638    2633    3016    4258    1638 
   Size:        995     383    1242    4618

  Fragments arranged by size: 

                4618    1242     995     383
 
MfeI C'AATT_G

Cuts at:   2444    2444 
   Size:       7238
 
MluI A'CGCG_T

Cuts at:   6671    6671 
   Size:       7238
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1521    5909    6093    1521 
   Size:       4388     184    2666

  Fragments arranged by size: 

                4388    2666     184
 
MscI TGG'CCA

Cuts at:    640    1708    2731    4999     640 
   Size:       1068    1023    2268    2879

  Fragments arranged by size: 

                2879    2268    1068    1023
 
MslI CAynn'nnrTG

Cuts at:   1154    1629    2725    5227    5354    1154 
   Size:        475    1096    2502     127    3038

  Fragments arranged by size: 

                3038    2502    1096     475     127
 
MspA1I CmG'CkG

Cuts at:    243     289     988    1432    2974    4349    4663    5023    6036 
   Size:         46     699     444    1542    1375     314     360    1013

Cuts at:   6036    6281     243 
   Size:        245    1200

  Fragments arranged by size: 

                1542    1375    1200    1013     699     444     360     314
                 245      46
 
NaeI GCC'GGC

Cuts at:   5419    5419 
   Size:       7238
 
NarI GG'CG_CC

Cuts at:    479    4669    4917     479 
   Size:       4190     248    2800

  Fragments arranged by size: 

                4190    2800     248
 
NcoI C'CATG_G

Cuts at:   1238    1347    3474    1238 
   Size:        109    2127    5002

  Fragments arranged by size: 

                5002    2127     109
 
NdeI CA'TA_TG

Cuts at:    390     390 
   Size:       7238
 
NgoMIV G'CCGG_C

Cuts at:   5417    5417 
   Size:       7238
 
NsiI A_TGCA'T

Cuts at:   1176    1176 
   Size:       7238
 
NspI r_CATG'y

Cuts at:   4291    4791    5698    4291 
   Size:        500     907    5831

  Fragments arranged by size: 

                5831     907     500
 
PciI A'CATG_T

Cuts at:   5694    5694 
   Size:       7238
 
PflMI CCAn_nnn'nTGG

Cuts at:   2328    2643    2328 
   Size:        315    6923
 
PfoI T'CCnGG_A

Cuts at:    923    1079     923 
   Size:        156    7082
 
PmeI GTTT'AAAC

Cuts at:   4237    4237 
   Size:       7238
 
PmlI CAC'GTG

Cuts at:   1878    4147    1878 
   Size:       2269    4969
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6377    6409    6377 
   Size:         32    7206
 
PpuMI rG'GwC_Cy

Cuts at:   2376    2376 
   Size:       7238
 
PspXI vC'TCGA_Gb

Cuts at:    324     324 
   Size:       7238
 
PstI C_TGCA'G

Cuts at:    199     449     686    2835    4285    4970     199 
   Size:        250     237    2149    1450     685    2467

  Fragments arranged by size: 

                2467    2149    1450     685     250     237
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7238
 
PvuII CAG'CTG

Cuts at:    243     988    1432    2974    4663    5023     243 
   Size:        745     444    1542    1689     360    2458

  Fragments arranged by size: 

                2458    1689    1542     745     444     360
 
RsrII CG'GwC_CG

Cuts at:   5433    5433 
   Size:       7238
 
SacI G_AGCT'C

Cuts at:    818    1463    4252     818 
   Size:        645    2789    3804

  Fragments arranged by size: 

                3804    2789     645
 
SalI G'TCGA_C

Cuts at:   4275    4275 
   Size:       7238
 
SapI GCTCTTCn'nnn_

Cuts at:   5261    5471    5261 
   Size:        210    7028
 
SbfI CC_TGCA'GG

Cuts at:   4285    4285 
   Size:       7238
 
ScaI AGT'ACT

Cuts at:    919    6755     919 
   Size:       5836    1402
 
SfcI C'TryA_G

Cuts at:     33     195     445     682    1068    1393    2547    2831    4281 
   Size:        162     250     237     386     325    1154     284    1450

Cuts at:   4281    4966    5959    6150      33 
   Size:        685     993     191    1121

  Fragments arranged by size: 

                1450    1154    1121     993     685     386     325     284
                 250     237     191     162
 
SfoI GGC'GCC

Cuts at:    480    4670    4918     480 
   Size:       4190     248    2800

  Fragments arranged by size: 

                4190    2800     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7238
 
SmaI CCC'GGG

Cuts at:   4260    4260 
   Size:       7238
 
SmlI C'TyrA_G

Cuts at:    324    1639    2205    2560    3967    4164    4393    5308    5800 
   Size:       1315     566     355    1407     197     229     915     492

Cuts at:   5800    6062     324 
   Size:        262    1500

  Fragments arranged by size: 

                1500    1407    1315     915     566     492     355     262
                 229     197
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       7238
 
SphI G_CATG'C

Cuts at:   4291    4791    4291 
   Size:        500    6738
 
SspI AAT'ATT

Cuts at:   1796    2316    1796 
   Size:        520    6718
 
StyI C'CwwG_G

Cuts at:    411     612    1238    1347    2840    3474    4372    4726     411 
   Size:        201     626     109    1493     634     898     354    2923

  Fragments arranged by size: 

                2923    1493     898     634     626     354     201     109
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   3431    4821    4954    5413    5622    3431 
   Size:       1390     133     459     209    5047

  Fragments arranged by size: 

                5047    1390     459     209     133
 
TatI w'GTAC_w

Cuts at:    917    2020    2611    2713    3837    6753     917 
   Size:       1103     591     102    1124    2916    1402

  Fragments arranged by size: 

                2916    1402    1124    1103     591     102
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    729     761    1905    1937    3237    3269    6755    6787     729 
   Size:         32    1144      32    1300      32    3486      32    1180

  Fragments arranged by size: 

                3486    1300    1180    1144      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   2155    5035    2155 
   Size:       2880    4358
 
XbaI T'CTAG_A

Cuts at:   4269    4269 
   Size:       7238
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3615    3873    4733    3615 
   Size:        258     860    6120

  Fragments arranged by size: 

                6120     860     258
 
XhoI C'TCGA_G

Cuts at:    324     324 
   Size:       7238
 
XmaI C'CCGG_G

Cuts at:   4258    4258 
   Size:       7238
 
XmnI GAAnn'nnTTC

Cuts at:   2350    2391    3887    2350 
   Size:         41    1496    5701

  Fragments arranged by size: 

                5701    1496      41
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AhdI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PspXI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BcgI     BglI    BmgBI     BmtI    BsiWI    BsmBI
   BssHII   BstEII     ClaI    CspCI     FseI    FspAI     HpaI     NheI
     NotI     NruI     PacI     PasI    PshAI     PsiI   PspOMI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI
     SwaI     ZraI
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