Restriction map for pF1KB3720
 (Linear) (Six-base) MAPSORT of: KIBB3720.seq  Check: 4583  from: 1  to: 972



KIBB3720.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AleI CACnn'nnGTG

Cuts at:      0     164     745     972 
   Size:        164     581     227

  Fragments arranged by size: 

                 581     227     164
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     170     972 
   Size:        170     802
 
AlwNI CAG_nnn'CTG

Cuts at:      0     558     972 
   Size:        558     414
 
ApaI G_GGCC'C

Cuts at:      0     942     972 
   Size:        942      30
 
ApoI r'AATT_y

Cuts at:      0     376     972 
   Size:        376     596
 
AvaI C'yCGr_G

Cuts at:      0     635     972 
   Size:        635     337
 
AvrII C'CTAG_G

Cuts at:      0     958     972 
   Size:        958      14
 
BanI G'GyrC_C

Cuts at:      0     739     842     972 
   Size:        739     103     130

  Fragments arranged by size: 

                 739     130     103
 
BanII G_rGCy'C

Cuts at:      0     877     942     972 
   Size:        877      65      30

  Fragments arranged by size: 

                 877      65      30
 
BglI GCCn_nnn'nGGC

Cuts at:      0      67     712     972 
   Size:         67     645     260

  Fragments arranged by size: 

                 645     260      67
 
Bme1580I G_kGCm'C

Cuts at:      0      85     615     636     742     797     942     972 
   Size:         85     530      21     106      55     145      30

  Fragments arranged by size: 

                 530     145     106      85      55      30      21
 
BmgBI CAC'GTC

Cuts at:      0     854     972 
   Size:        854     118
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     345     904     972 
   Size:        345     559      68

  Fragments arranged by size: 

                 559     345      68
 
BmtI G_CTAG'C

Cuts at:      0     257     667     972 
   Size:        257     410     305

  Fragments arranged by size: 

                 410     305     257
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     298     752     972 
   Size:        183     115     454     220

  Fragments arranged by size: 

                 454     220     183     115
 
BsaAI yAC'GTr

Cuts at:      0     621     972 
   Size:        621     351
 
BsaWI w'CCGG_w

Cuts at:      0     134     908     972 
   Size:        134     774      64

  Fragments arranged by size: 

                 774     134      64
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     469     499     972 
   Size:        469      30     473

  Fragments arranged by size: 

                 473     469      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     223     251     715     972 
   Size:        223      28     464     257

  Fragments arranged by size: 

                 464     257     223      28
 
BseYI C'CCAG_C

Cuts at:      0     544     562     594     935     972 
   Size:        544      18      32     341      37

  Fragments arranged by size: 

                 544     341      37      32      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     207     972 
   Size:        140      67     765

  Fragments arranged by size: 

                 765     140      67
 
BsmI GAATG_Cn'

Cuts at:      0     421     972 
   Size:        421     551
 
Bsp1286I G_dGCh'C

Cuts at:      0      85     615     636     742     797     877     942     972 
   Size:         85     530      21     106      55      80      65      30

  Fragments arranged by size: 

                 530     106      85      80      65      55      30      21
            
 
BspEI T'CCGG_A

Cuts at:      0     908     972 
   Size:        908      64
 
BsrBI CCG'CTC

Cuts at:      0     480     972 
   Size:        480     492
 
BsrDI GCAATG_nn'

Cuts at:      0     818     972 
   Size:        818     154
 
BssHII G'CGCG_C

Cuts at:      0      11     972 
   Size:         11     961
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      30     496     972 
   Size:         30     466     476

  Fragments arranged by size: 

                 476     466      30
 
BtgI C'CryG_G

Cuts at:      0     344     534     797     972 
   Size:        344     190     263     175

  Fragments arranged by size: 

                 344     263     190     175
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     438     972 
   Size:        438     534
 
BtsI GCAGTG_nn'

Cuts at:      0     219     972 
   Size:        219     753
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     298     752     972 
   Size:        183     115     454     220

  Fragments arranged by size: 

                 454     220     183     115
 
EcoO109I rG'GnC_Cy

Cuts at:      0     555     714     922     972 
   Size:        555     159     208      50

  Fragments arranged by size: 

                 555     208     159      50
 
FspI TGC'GCA

Cuts at:      0     289     972 
   Size:        289     683
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     183     215     491     523     972 
   Size:        183      32     276      32     449

  Fragments arranged by size: 

                 449     276     183      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     972 
   Size:        467     505
 
MslI CAynn'nnrTG

Cuts at:      0     164     469     745     972 
   Size:        164     305     276     227

  Fragments arranged by size: 

                 305     276     227     164
 
MspA1I CmG'CkG

Cuts at:      0     839     972 
   Size:        839     133
 
NcoI C'CATG_G

Cuts at:      0     534     972 
   Size:        534     438
 
NdeI CA'TA_TG

Cuts at:      0     696     809     972 
   Size:        696     113     163

  Fragments arranged by size: 

                 696     163     113
 
NheI G'CTAG_C

Cuts at:      0     253     663     972 
   Size:        253     410     309

  Fragments arranged by size: 

                 410     309     253
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     366     972 
   Size:        366     606
 
PfoI T'CCnGG_A

Cuts at:      0     644     972 
   Size:        644     328
 
PpuMI rG'GwC_Cy

Cuts at:      0     922     972 
   Size:        922      50
 
PspOMI G'GGCC_C

Cuts at:      0     938     972 
   Size:        938      34
 
PstI C_TGCA'G

Cuts at:      0     554     677     972 
   Size:        554     123     295

  Fragments arranged by size: 

                 554     295     123
 
PvuII CAG'CTG

Cuts at:      0     839     972 
   Size:        839     133
 
SbfI CC_TGCA'GG

Cuts at:      0     554     972 
   Size:        554     418
 
ScaI AGT'ACT

Cuts at:      0     295     972 
   Size:        295     677
 
SexAI A'CCwGG_T

Cuts at:      0     107     972 
   Size:        107     865
 
SfcI C'TryA_G

Cuts at:      0     550     673     834     972 
   Size:        550     123     161     138

  Fragments arranged by size: 

                 550     161     138     123
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     712     972 
   Size:        712     260
 
StyI C'CwwG_G

Cuts at:      0     381     534     743     958     972 
   Size:        381     153     209     215      14

  Fragments arranged by size: 

                 381     215     209     153      14
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     885     972 
   Size:        885      87
 
TatI w'GTAC_w

Cuts at:      0     171     293     972 
   Size:        171     122     679

  Fragments arranged by size: 

                 679     171     122
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     325     357     469     501     972 
   Size:        325      32     112      32     471

  Fragments arranged by size: 

                 471     325     112      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     891     972 
   Size:        891      81
 

 Enzymes that do cut:

     AleI     AlfI    AlwNI     ApaI     ApoI     AvaI    AvrII     BanI
    BanII     BglI Bme1580I    BmgBI     BmrI     BmtI     BpmI    BsaAI
    BsaWI    BsaXI    BseRI    BseYI     BsgI     BsmI Bsp1286I    BspEI
    BsrBI    BsrDI   BssHII    BstXI     BtgI    BtgZI     BtsI  Eco57MI
 EcoO109I     FspI    Hin4I     MmeI     MslI   MspA1I     NcoI     NdeI
     NheI    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII     SbfI
     ScaI    SexAI     SfcI     SfiI     StyI    TaqII     TatI     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AloI    ApaLI     AscI     AseI    AsiSI
     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI     BplI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI    BspMI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI   NgoMIV     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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