Restriction map for pF1KB3707
 (Circular) (Six-base) MAPSORT of: pF1KB3707.seq  Check: 1849  from: 1  to: 6110



pF1KB3707  6110 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1233    1953    1233 
   Size:        720    5390
 
AccI GT'mk_AC

Cuts at:   1433    3148    1433 
   Size:       1715    4395
 
Acc65I G'GTAC_C

Cuts at:   1870    3126    1870 
   Size:       1256    4854
 
AclI AA'CG_TT

Cuts at:   2674    5950    2674 
   Size:       3276    2834
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    611    1318    1513    1643    2260    2972    3935    4367    4720 
   Size:        707     195     130     617     712     963     432     353

Cuts at:   4720    5382     611 
   Size:        662    1339

  Fragments arranged by size: 

                1339     963     712     707     662     617     432     353
                 195     130
 
AflIII A'CryG_T

Cuts at:   3462    5262    5543    3462 
   Size:       1800     281    4029

  Fragments arranged by size: 

                4029    1800     281
 
AgeI A'CCGG_T

Cuts at:    312     312 
   Size:       6110
 
AhdI GACnn_n'nnGTC

Cuts at:   1710    1710 
   Size:       6110
 
AleI CACnn'nnGTG

Cuts at:    832    2479    2659     832 
   Size:       1647     180    4283

  Fragments arranged by size: 

                4283    1647     180
 
AlwNI CAG_nnn'CTG

Cuts at:   1653    2128    2820    2977    3049    3415    4853    1653 
   Size:        475     692     157      72     366    1438    2910

  Fragments arranged by size: 

                2910    1438     692     475     366     157      72
 
ApaI G_GGCC'C

Cuts at:   1532    2625    1532 
   Size:       1093    5017
 
ApaLI G'TGCA_C

Cuts at:    508    4948     508 
   Size:       4440    1670
 
ApoI r'AATT_y

Cuts at:   1147    3114    5463    5719    1147 
   Size:       1967    2349     256    1538

  Fragments arranged by size: 

                2349    1967    1538     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6110
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6110
 
AvaI C'yCGr_G

Cuts at:   1268    2377    2879    3130    1268 
   Size:       1109     502     251    4248

  Fragments arranged by size: 

                4248    1109     502     251
 
BamHI G'GATC_C

Cuts at:   2995    3135    2995 
   Size:        140    5970
 
BanI G'GyrC_C

Cuts at:   1182    1870    3126    3540    3788    3823    1182 
   Size:        688    1256     414     248      35    3469

  Fragments arranged by size: 

                3469    1256     688     414     248      35
 
BanII G_rGCy'C

Cuts at:    811     938    1532    1930    1973    2625    2966    3124    4154 
   Size:        127     594     398      43     652     341     158    1030

Cuts at:   4154     811 
   Size:       2767

  Fragments arranged by size: 

                2767    1030     652     594     398     341     158     127
                  43
 
BbeI G_GCGC'C

Cuts at:   3544    3792    3544 
   Size:        248    5862
 
BbsI GAAGACnn'nnnn_

Cuts at:    633     744     859     633 
   Size:        111     115    5884

  Fragments arranged by size: 

                5884     115     111
 
BbvCI CC'TCA_GC

Cuts at:    739    1985    2509     739 
   Size:       1246     524    4340

  Fragments arranged by size: 

                4340    1246     524
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    664     698     664 
   Size:         34    6076
 
BciVI GTATCCnnnnn_n'

Cuts at:   1709    4008    5064    6110    1709 
   Size:       2299    1056    1046    1709

  Fragments arranged by size: 

                2299    1709    1056    1046
 
BclI T'GATC_A

Cuts at:    626    3630     626 
   Size:       3004    3106
 
BglI GCCn_nnn'nGGC

Cuts at:    141     163    1360    2568    2731     141 
   Size:         22    1197    1208     163    3520

  Fragments arranged by size: 

                3520    1208    1197     163      22
 
BglII A'GATC_T

Cuts at:   1626    2417    2648    3625    5662    1626 
   Size:        791     231     977    2037    2074

  Fragments arranged by size: 

                2074    2037     977     791     231
 
BlpI GC'TnA_GC

Cuts at:   1384    2431    2504    2735    3222    1384 
   Size:       1047      73     231     487    4272

  Fragments arranged by size: 

                4272    1047     487     231      73
 
Bme1580I G_kGCm'C

Cuts at:    272     512    1532    2625    3735    3828    4952     272 
   Size:        240    1020    1093    1110      93    1124    1430

  Fragments arranged by size: 

                1430    1124    1110    1093    1020     240      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1987    3322    3403    3738    5882    1987 
   Size:       1335      81     335    2144    2215

  Fragments arranged by size: 

                2215    2144    1335     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2022    2436    4477    2022 
   Size:        414    2041    3655

  Fragments arranged by size: 

                3655    2041     414
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    284     946    1192    2126    2320    2473    2921    3059     284 
   Size:        662     246     934     194     153     448     138    3335

  Fragments arranged by size: 

                3335     934     662     448     246     194     153     138
            
 
Bpu10I CC'TnA_GC

Cuts at:    739     810    1985    2509     739 
   Size:         71    1175     524    4340

  Fragments arranged by size: 

                4340    1175     524      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1957    2599    3286    4165    4879    5177    1957 
   Size:        642     687     879     714     298    2890

  Fragments arranged by size: 

                2890     879     714     687     642     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2288    2347    2689    5814    2288 
   Size:         59     342    3125    2584

  Fragments arranged by size: 

                3125    2584     342      59
 
BsaAI yAC'GTr

Cuts at:   3463    3463 
   Size:       6110
 
BsaBI GATnn'nnATC

Cuts at:      6    3624    5661       6 
   Size:       3618    2037     455

  Fragments arranged by size: 

                3618    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2135    3541    3789    4491    5987    2135 
   Size:       1406     248     702    1496    2258

  Fragments arranged by size: 

                2258    1496    1406     702     248
 
BsaWI w'CCGG_w

Cuts at:    312    3299    3820    4498    4909    5056    5330     312 
   Size:       2987     521     678     411     147     274    1092

  Fragments arranged by size: 

                2987    1092     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    153    1168    1330    1483    1486    1936    2212    2416    2428 
   Size:       1015     162     153       3     450     276     204      12

Cuts at:   2428    2560    2581     153 
   Size:        132      21    3682

  Fragments arranged by size: 

                3682    1015     450     276     204     162     153     132
                  21      12       3
 
BseYI C'CCAG_C

Cuts at:    865     958    1320    3535    4293    4958    5366     865 
   Size:         93     362    2215     758     665     408    1609

  Fragments arranged by size: 

                2215    1609     758     665     408     362      93
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    380    1184    2287    2833     380 
   Size:        804    1103     546    3657

  Fragments arranged by size: 

                3657    1103     804     546
 
BsiEI CG_ry'CG

Cuts at:    104    3652    3698    4928     104 
   Size:       3548      46    1230    1286

  Fragments arranged by size: 

                3548    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    173     476     512     938    1760    1973    3124    3902    4092 
   Size:        303      36     426     822     213    1151     778     190

Cuts at:   4092    4952     173 
   Size:        860    1331

  Fragments arranged by size: 

                1331    1151     860     822     778     426     303     213
                 190      36
 
BsmI GAATG_Cn'

Cuts at:   2718    2718 
   Size:       6110
 
Bsp1286I G_dGCh'C

Cuts at:    173     272     476     512     811     938    1532    1760    1930 
   Size:         99     204      36     299     127     594     228     170

Cuts at:   1930    1973    2625    2966    3124    3735    3828    3902    4092 
   Size:         43     652     341     158     611      93      74     190

Cuts at:   4092    4154    4952     173 
   Size:         62     798    1331

  Fragments arranged by size: 

                1331     798     652     611     594     341     299     228
                 204     190     170     158     127      99      93      74
                  62      43      36
 
BspHI T'CATG_A

Cuts at:   4542    4542 
   Size:       6110
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1233    1953    2237    3160    3374    3676    4057    1233 
   Size:        720     284     923     214     302     381    3286

  Fragments arranged by size: 

                3286     923     720     381     302     284     214
 
BsrBI CCG'CTC

Cuts at:     50    1179    4402    4456    5940    6105      50 
   Size:       1129    3223      54    1484     165      55

  Fragments arranged by size: 

                3223    1484    1129     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   4022    4022 
   Size:       6110
 
BsrFI r'CCGG_y

Cuts at:    312    4108    4289     312 
   Size:       3796     181    2133

  Fragments arranged by size: 

                3796    2133     181
 
BsrGI T'GTAC_A

Cuts at:   1194    1194 
   Size:       6110
 
BssSI C'ACGA_G

Cuts at:    475    1139    1455    4381    5089     475 
   Size:        664     316    2926     708    1496

  Fragments arranged by size: 

                2926    1496     708     664     316
 
BstAPI GCAn_nnn'nTGC

Cuts at:    507     824    2820    3487     507 
   Size:        317    1996     667    3130

  Fragments arranged by size: 

                3130    1996     667     317
 
BstBI TT'CG_AA

Cuts at:   4471    4471 
   Size:       6110
 
BstXI CCAn_nnnn'nTGG

Cuts at:    246    1446    2334    2451     246 
   Size:       1200     888     117    3905

  Fragments arranged by size: 

                3905    1200     888     117
 
BstYI r'GATC_y

Cuts at:      1     887    1626    2417    2648    2995    3135    3602    3625 
   Size:        886     739     791     231     347     140     467      23

Cuts at:   3625    3960    4206    4512    4524    4610    5662       1 
   Size:        335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1052     886     791     739     467     449     347     335
                 306     246     231     140      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    545    2030    2048    2129    2861     545 
   Size:       1485      18      81     732    3794

  Fragments arranged by size: 

                3794    1485     732      81      18
 
BtgI C'CryG_G

Cuts at:    107     272    1022    1304    1355     107 
   Size:        165     750     282      51    4862

  Fragments arranged by size: 

                4862     750     282     165      51
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4060    4241    4404    4060 
   Size:        181     163    5766

  Fragments arranged by size: 

                5766     181     163
 
BtsI GCAGTG_nn'

Cuts at:    215     237     629     731     776    1370    1433    3353     215 
   Size:         22     392     102      45     594      63    1920    2972

  Fragments arranged by size: 

                2972    1920     594     392     102      63      45      22
            
 
DraI TTT'AAA

Cuts at:   1398    3109    1398 
   Size:       1711    4399
 
DraIII CAC_nnn'GTG

Cuts at:    196     196 
   Size:       6110
 
DrdI GACnn_nn'nnGTC

Cuts at:   3816    5160    3816 
   Size:       1344    4766
 
EaeI y'GGCC_r

Cuts at:    248    1025    2723    3695    3869    4260    4287    5632     248 
   Size:        777    1698     972     174     391      27    1345     726

  Fragments arranged by size: 

                1698    1345     972     777     726     391     174      27
            
 
EagI C'GGCC_G

Cuts at:   3695    3695 
   Size:       6110
 
EarI CTCTTCn'nnn_

Cuts at:   1519    1808    1913    4133    4343    1519 
   Size:        289     105    2220     210    3286

  Fragments arranged by size: 

                3286    2220     289     210     105
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    745    4318    5060    5206    5920     745 
   Size:       3573     742     146     714     935

  Fragments arranged by size: 

                3573     935     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    936    1971    3122     936 
   Size:       1035    1151    3924

  Fragments arranged by size: 

                3924    1151    1035
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    284     611     946    1192    1318    1513    1643    2126    2260 
   Size:        327     335     246     126     195     130     483     134

Cuts at:   2260    2320    2473    2921    2972    3059    3935    4367    4720 
   Size:         60     153     448      51      87     876     432     353

Cuts at:   4720    5382     284 
   Size:        662    1012

  Fragments arranged by size: 

                1012     876     662     483     448     432     353     335
                 327     246     195     153     134     130     126      87
                  60      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2939    2939 
   Size:       6110
 
EcoO109I rG'GnC_Cy

Cuts at:    921    1203    1272    1528    1529    1618    1989    2125    2621 
   Size:        282      69     256       1      89     371     136     496

Cuts at:   2621    2876    3034    3249     921 
   Size:        255     158     215    3782

  Fragments arranged by size: 

                3782     496     371     282     256     255     215     158
                 136      89      69       1
 
EcoRI G'AATT_C

Cuts at:   3114    3114 
   Size:       6110
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1749    4129    1749 
   Size:       2380    3730
 
FspI TGC'GCA

Cuts at:   3891    3891 
   Size:       6110
 
HaeII r_GCGC'y

Cuts at:    663    2438    3544    3792    5022    5793     663 
   Size:       1775    1106     248    1230     771     980

  Fragments arranged by size: 

                1775    1230    1106     980     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    366     398     793     825    1696    1728    2980    3012    3952 
   Size:         32     395      32     871      32    1252      32     940

Cuts at:   3952    3982    3984    4014     366 
   Size:         30       2      30    2462

  Fragments arranged by size: 

                2462    1252     940     871     395      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   3149    3149 
   Size:       6110
 
KasI G'GCGC_C

Cuts at:   3540    3788    3540 
   Size:        248    5862
 
KpnI G_GTAC'C

Cuts at:   1874    3130    1874 
   Size:       1256    4854
 
MluI A'CGCG_T

Cuts at:   5543    5543 
   Size:       6110
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2448    4869    5053    2448 
   Size:       2421     184    3505

  Fragments arranged by size: 

                3505    2421     184
 
MscI TGG'CCA

Cuts at:    250    1027    3871     250 
   Size:        777    2844    2489

  Fragments arranged by size: 

                2844    2489     777
 
MslI CAynn'nnrTG

Cuts at:    244     832    1444    2479    2482    2659    4099    4226     244 
   Size:        588     612    1035       3     177    1440     127    2128

  Fragments arranged by size: 

                2128    1440    1035     612     588     177     127       3
            
 
MspA1I CmG'CkG

Cuts at:    531     882    1378    3221    3535    3895    4679    4924     531 
   Size:        351     496    1843     314     360     784     245    1717

  Fragments arranged by size: 

                1843    1717     784     496     360     351     314     245
            
 
NaeI GCC'GGC

Cuts at:   4291    4291 
   Size:       6110
 
NarI GG'CG_CC

Cuts at:   3541    3789    3541 
   Size:        248    5862
 
NcoI C'CATG_G

Cuts at:    107     272    1022    1355     107 
   Size:        165     750     333    4862

  Fragments arranged by size: 

                4862     750     333     165
 
NgoMIV G'CCGG_C

Cuts at:   4289    4289 
   Size:       6110
 
NspI r_CATG'y

Cuts at:   2610    3163    3663    5266    2610 
   Size:        553     500    1603    3454

  Fragments arranged by size: 

                3454    1603     553     500
 
PasI CC'CwG_GG

Cuts at:    915     915 
   Size:       6110
 
PciI A'CATG_T

Cuts at:   5262    5262 
   Size:       6110
 
PfoI T'CCnGG_A

Cuts at:    467    1910    3036     467 
   Size:       1443    1126    3541

  Fragments arranged by size: 

                3541    1443    1126
 
PmeI GTTT'AAAC

Cuts at:   3109    3109 
   Size:       6110
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4556    4588    4556 
   Size:         32    6078
 
PpuMI rG'GwC_Cy

Cuts at:   1272    2876    3034    1272 
   Size:       1604     158    4348

  Fragments arranged by size: 

                4348    1604     158
 
PspOMI G'GGCC_C

Cuts at:   1528    2621    1528 
   Size:       1093    5017
 
PstI C_TGCA'G

Cuts at:    536     677     710     887    1935    3157    3842     536 
   Size:        141      33     177    1048    1222     685    2804

  Fragments arranged by size: 

                2804    1222    1048     685     177     141      33
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6110
 
PvuII CAG'CTG

Cuts at:    531     882    1378    3535    3895     531 
   Size:        351     496    2157     360    2746

  Fragments arranged by size: 

                2746    2157     496     360     351
 
RsrII CG'GwC_CG

Cuts at:   4305    4305 
   Size:       6110
 
SacI G_AGCT'C

Cuts at:    938    1973    3124     938 
   Size:       1035    1151    3924

  Fragments arranged by size: 

                3924    1151    1035
 
SalI G'TCGA_C

Cuts at:   3147    3147 
   Size:       6110
 
SanDI GG'GwC_CC

Cuts at:   1272    2876    1272 
   Size:       1604    4506
 
SapI GCTCTTCn'nnn_

Cuts at:   4133    4343    4133 
   Size:        210    5900
 
SbfI CC_TGCA'GG

Cuts at:   3157    3157 
   Size:       6110
 
ScaI AGT'ACT

Cuts at:   5627    5627 
   Size:       6110
 
SfcI C'TryA_G

Cuts at:     33     532     673     706     883    1566    1931    3153    3838 
   Size:        499     141      33     177     683     365    1222     685

Cuts at:   3838    4806    4997      33 
   Size:        968     191    1146

  Fragments arranged by size: 

                1222    1146     968     685     683     499     365     191
                 177     141      33
 
SfoI GGC'GCC

Cuts at:   3542    3790    3542 
   Size:        248    5862
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6110
 
SgrAI Cr'CCGG_yG

Cuts at:    312     312 
   Size:       6110
 
SmaI CCC'GGG

Cuts at:   2881    3132    2881 
   Size:        251    5859
 
SmlI C'TyrA_G

Cuts at:   1972    2614    3265    4180    4894    5156    1972 
   Size:        642     651     915     714     262    2926

  Fragments arranged by size: 

                2926     915     714     651     642     262
 
SpeI A'CTAG_T

Cuts at:     67    3102      67 
   Size:       3035    3075
 
SphI G_CATG'C

Cuts at:   3163    3663    3163 
   Size:        500    5610
 
StuI AGG'CCT

Cuts at:   1103    1103 
   Size:       6110
 
StyI C'CwwG_G

Cuts at:    107     158     272    1022    1355    1715    1886    2523    3244 
   Size:         51     114     750     333     360     171     637     721

Cuts at:   3244    3598     107 
   Size:        354    2619

  Fragments arranged by size: 

                2619     750     721     637     360     354     333     171
                 114      51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1246    1669    2819    3693    3826    4285    4494    1246 
   Size:        423    1150     874     133     459     209    2862

  Fragments arranged by size: 

                2862    1150     874     459     423     209     133
 
TatI w'GTAC_w

Cuts at:   1194    5625    1194 
   Size:       4431    1679
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5627    5659    5627 
   Size:         32    6078
 
Tth111I GACn'n_nGTC

Cuts at:   3907    3907 
   Size:       6110
 
XbaI T'CTAG_A

Cuts at:   1764    3141    1764 
   Size:       1377    4733
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3605    3605 
   Size:       6110
 
XmaI C'CCGG_G

Cuts at:   2879    3130    2879 
   Size:        251    5859
 
XmnI GAAnn'nnTTC

Cuts at:   1628    1628 
   Size:       6110
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AleI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI     PfoI     PmeI     PpiI    PpuMI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AlfI     AloI     AscI    AvrII     BaeI
    BfrBI    BmgBI     BmtI    BsaXI    BsiWI    BsmBI    BspEI   BssHII
   BstEII  BstZ17I     ClaI    CspCI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
    PflMI     PmlI    PshAI     PsiI    PspXI     PsrI    SacII    SexAI
     SfiI    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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