Restriction map for pF1KB3705
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       AccII       AcyI        AgeI        AocI        
Aor51HI     ApaI        AscI        AspI        AvrII       
BbeI        BbrPI       BbvCI       BciVI       BlnI        
BsaAI       BsaHI       BsaI        BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
BssSI       BstAPI      BstEII      BstPI       BstUI       
Bsu36I      ClaI        CpoI        DraIII      DrdI        
DsaI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
FseI        HgaI        Hin1I       HindIII     HpaI        
KasI        KspI        MluI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        135  GGTACC               G^GTACC
AccI         2365  GTATAC               GT^[AC][GT]AC
AccIII        806  TCCGGA               T^CCGGA
AclI          689  AACGTT               AA^CGTT
AflII        3070  CTTAAG               C^TTAAG
AflIII        623  ACGTGT               A^C[GA][CT]GT
AhdI         2269  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2044  GTGCAC               G^TGCAC
AlwNI        1774  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        2044  GTGCAC               G^TGCAC
Asp718I       135  GGTACC               G^GTACC
AspEI        2269  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII        2682  GGACC                G^G[AT]CC
AviII         455  TGCGCA               TGC^GCA
BalI         2790  TGGCCA               TGG^CCA
BanI          135  GGTACC               G^G[CT][GA]CC
BcgI         1684  CGACAAGACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         3070  CTTAAG               C^TTAAG
BglII        3205  AGATCT               A^GATCT
BlpI         1765  GCTCAGC              GC^T[ACGT]AGC
BpmI         1361  CTGGAG               CTGGAG(16/14)
Bpu1102I     1765  GCTCAGC              GC^T[ACGT]AGC
BsaWI         806  TCCGGA               [AT]^CCGG[AT]
BseAI         806  TCCGGA               T^CCGGA
BseRI         268  GAGGAG               GAGGAG(10/8)
BspEI         806  TCCGGA               T^CCGGA
BsrDI        2603  GCAATG               GCAATG(2/0)
BsrFI        2939  GCCGGT               [GA]^CCGG[CT]
Bst1107I     2365  GTATAC               GTA^TAC
BstBI         443  TTCGAA               TT^CGAA
BstXI          86  CCATACCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2365  GTATAC               GTA^TAC
CelII        1765  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       2939  GCCGGT               [GA]^CCGG[CT]
DraII        1814  AGGCCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I     2269  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1814  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I      2742  CCTTGG               C^C[AT][AT]GG
FauI          752  GCGGG                CCCGC(4/6)
FspI          455  TGCGCA               TGC^GCA
KpnI          135  GGTACC               GGTAC^C
MluNI        2790  TGGCCA               TGG^CCA
MroI          806  TCCGGA               T^CCGGA
MscI         2790  TGGCCA               TGG^CCA
NspV          443  TTCGAA               TT^CGAA
Psp1406I      689  AACGTT               AA^CGTT
SacI         1084  GAGCTC               GAGCT^C
SapI         2796  GCTCTTC              GCTCTTC(1/4)
SfuI          443  TTCGAA               TT^CGAA
SmaI         1808  CCCGGG               CCC^GGG
StyI         2742  CCTTGG               C^C[AT][AT]GG
XbaI         1969  TCTAGA               T^CTAGA
XcmI         2802  CCACATTGACTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1808  CCCGGG               C^CCGGG
XmaI         1808  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AseI         1165  ATTAAT               AT^TAAT
             1258  ATTAAT              
AsnI         1165  ATTAAT               AT^TAAT
             1258  ATTAAT              
Asp700I       962  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2991  GAATCTCTTC          
AvaI          140  CTCGGG               C^[CT]CG[GA]G
             1808  CCCGGG              
BamHI         423  GGATCC               G^GATCC
             1632  GGATCC              
BanII         827  GAGCCC               G[GA]GC[CT]^C
             1084  GAGCTC              
BbsI          290  GAAGAC               GAAGAC(2/6)
             1386  GTCTTC              
BclI          777  TGATCA               T^GATCA
             1131  TGATCA              
BfaI         1819  CTAG                 C^TAG
             1970  CTAG                
BglI          895  GCCATAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2352  GCCTTTGTGGC         
BpuAI         290  GAAGAC               GAAGAC(2/6)
             1386  GTCTTC              
BsaBI         117  GATTTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2962  GATGCATATC          
BsmI          853  GAATGC               GAATGC(1/-1)
             2184  GAATGC              
BsoBI         140  CTCGGG               C^[CT]CG[GA]G
             1808  CCCGGG              
BspMI        2270  ACCTGC               ACCTGC(4/8)
             2133  GCAGGT              
EaeI         2790  TGGCCA               [CT]^GGCC[GA]
             2937  TGGCCG              
EcoT22I      2224  ATGCAT               ATGCA^T
             2963  ATGCAT              
FbaI          777  TGATCA               T^GATCA
             1131  TGATCA              
HaeII        1776  GGCGCT               [GA]GCGC^[CT]
             3141  GGCGCT              
HincII       2442  GTTGAC               GT[CT]^[GA]AC
             3021  GTTGAC              
HindII       2442  GTTGAC               GT[CT]^[GA]AC
             3021  GTTGAC              
MaeI         1819  CTAG                 C^TAG
             1970  CTAG                
MamI          117  GATTTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2962  GATGCATATC          
MfeI         1351  CAATTG               C^AATTG
             2576  CAATTG              
MslI           87  CATACCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2115  CATGAAAGTG          
MunI         1351  CAATTG               C^AATTG
             2576  CAATTG              
NsiI         2224  ATGCAT               ATGCA^T
             2963  ATGCAT              
NspI          241  GCATGT               [GA]CATG^[CT]
             1473  GCATGT              
PshBI        1165  ATTAAT               AT^TAAT
             1258  ATTAAT              
SmlI         1753  CTTGAG               C^T[CT][GA]AG
             3070  CTTAAG              
SspI         1168  AATATT               AAT^ATT
             1371  AATATT              
XmnI          962  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2991  GAATCTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       BbvI        BcnI        BmyI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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