Restriction map for pF1KB3702
 (Linear) (Six-base) MAPSORT of: KIBB3702.seq  Check: 8442  from: 1  to: 3114



KIBB3702.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     202    3114 
   Size:        202    2912
 
AccI GT'mk_AC

Cuts at:      0    1211    1564    2859    3114 
   Size:       1211     353    1295     255

  Fragments arranged by size: 

                1295    1211     353     255
 
AflII C'TTAA_G

Cuts at:      0    2941    3114 
   Size:       2941     173
 
AflIII A'CryG_T

Cuts at:      0    1214    2919    3114 
   Size:       1214    1705     195

  Fragments arranged by size: 

                1705    1214     195
 
AleI CACnn'nnGTG

Cuts at:      0     884    3114 
   Size:        884    2230
 
AlwNI CAG_nnn'CTG

Cuts at:      0     475     682    2779    3114 
   Size:        475     207    2097     335

  Fragments arranged by size: 

                2097     475     335     207
 
ApaI G_GGCC'C

Cuts at:      0    2529    3114 
   Size:       2529     585
 
ApoI r'AATT_y

Cuts at:      0     387     714    1752    2289    2314    3114 
   Size:        387     327    1038     537      25     800

  Fragments arranged by size: 

                1038     800     537     387     327      25
 
AseI AT'TA_AT

Cuts at:      0    2579    3114 
   Size:       2579     535
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1276    1309    3114 
   Size:       1276      33    1805

  Fragments arranged by size: 

                1805    1276      33
 
BanI G'GyrC_C

Cuts at:      0      30    1149    2951    3114 
   Size:         30    1119    1802     163

  Fragments arranged by size: 

                1802    1119     163      30
 
BanII G_rGCy'C

Cuts at:      0    1546    2306    2529    3114 
   Size:       1546     760     223     585

  Fragments arranged by size: 

                1546     760     585     223
 
BbeI G_GCGC'C

Cuts at:      0    2955    3114 
   Size:       2955     159
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     538    1448    1664    1988    3114 
   Size:        538     910     216     324    1126

  Fragments arranged by size: 

                1126     910     538     324     216
 
BbvCI CC'TCA_GC

Cuts at:      0    1490    3114 
   Size:       1490    1624
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     505    3114 
   Size:        505    2609
 
BglII A'GATC_T

Cuts at:      0    2222    3114 
   Size:       2222     892
 
Bme1580I G_kGCm'C

Cuts at:      0      35    2529    3114 
   Size:         35    2494     585

  Fragments arranged by size: 

                2494     585      35
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     900    3114 
   Size:        900    2214
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2378    3114 
   Size:       2378     736
 
Bpu10I CC'TnA_GC

Cuts at:      0    1490    3114 
   Size:       1490    1624
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1200    1411    2014    3114 
   Size:       1200     211     603    1100

  Fragments arranged by size: 

                1200    1100     603     211
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     260     524    1368    2253    3114 
   Size:        260     264     844     885     861

  Fragments arranged by size: 

                 885     861     844     264     260
 
BsaBI GATnn'nnATC

Cuts at:      0     116    2048    3114 
   Size:        116    1932    1066

  Fragments arranged by size: 

                1932    1066     116
 
BsaHI Gr'CG_yC

Cuts at:      0    2952    3114 
   Size:       2952     162
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     294     297     324     327    3114 
   Size:        294       3      27       3    2787

  Fragments arranged by size: 

                2787     294      27       3       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     302     977    1635    3114 
   Size:        302     675     658    1479

  Fragments arranged by size: 

                1479     675     658     302
 
BseYI C'CCAG_C

Cuts at:      0    1795    3114 
   Size:       1795    1319
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1463    3114 
   Size:       1463    1651
 
BsiHKAI G_wGCw'C

Cuts at:      0    2063    2805    3114 
   Size:       2063     742     309

  Fragments arranged by size: 

                2063     742     309
 
BsmI GAATG_Cn'

Cuts at:      0    1707    3114 
   Size:       1707    1407
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2607    3114 
   Size:       2607     507
 
Bsp1286I G_dGCh'C

Cuts at:      0      35    1546    2063    2306    2529    2805    3114 
   Size:         35    1511     517     243     223     276     309

  Fragments arranged by size: 

                1511     517     309     276     243     223      35
 
BspHI T'CATG_A

Cuts at:      0     156    3114 
   Size:        156    2958
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     202     987    1338    2419    3114 
   Size:        202     785     351    1081     695

  Fragments arranged by size: 

                1081     785     695     351     202
 
BsrDI GCAATG_nn'

Cuts at:      0     455     767    3114 
   Size:        455     312    2347

  Fragments arranged by size: 

                2347     455     312
 
BsrGI T'GTAC_A

Cuts at:      0    1258    3114 
   Size:       1258    1856
 
BssSI C'ACGA_G

Cuts at:      0     959    1354    3114 
   Size:        959     395    1760

  Fragments arranged by size: 

                1760     959     395
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1486    3114 
   Size:       1486    1628
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      57    1394    3114 
   Size:         57    1337    1720

  Fragments arranged by size: 

                1720    1337      57
 
BstYI r'GATC_y

Cuts at:      0     111     267     853    2222    2775    2823    3114 
   Size:        111     156     586    1369     553      48     291

  Fragments arranged by size: 

                1369     586     553     291     156     111      48
 
BstZ17I GTA'TAC

Cuts at:      0    2860    3114 
   Size:       2860     254
 
BtgI C'CryG_G

Cuts at:      0      43    3080    3114 
   Size:         43    3037      34

  Fragments arranged by size: 

                3037      43      34
 
BtsI GCAGTG_nn'

Cuts at:      0      65     800    1421    3041    3068    3114 
   Size:         65     735     621    1620      27      46

  Fragments arranged by size: 

                1620     735     621      65      46      27
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1914    1949    3114 
   Size:       1914      35    1165

  Fragments arranged by size: 

                1914    1165      35
 
DraI TTT'AAA

Cuts at:      0     108    1001    3114 
   Size:        108     893    2113

  Fragments arranged by size: 

                2113     893     108
 
DraIII CAC_nnn'GTG

Cuts at:      0    1486    2755    3114 
   Size:       1486    1269     359

  Fragments arranged by size: 

                1486    1269     359
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2995    3114 
   Size:       2995     119
 
EaeI y'GGCC_r

Cuts at:      0     627    2184    2496    2631    2677    2691    3114 
   Size:        627    1557     312     135      46      14     423

  Fragments arranged by size: 

                1557     627     423     312     135      46      14
 
EarI CTCTTCn'nnn_

Cuts at:      0    1273    2793    3114 
   Size:       1273    1520     321

  Fragments arranged by size: 

                1520    1273     321
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2378    3114 
   Size:       2378     736
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2525    3114 
   Size:       2525     589
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2761    3114 
   Size:       2761     353
 
HaeII r_GCGC'y

Cuts at:      0    2955    3114 
   Size:       2955     159
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1177    1209    2214    2246    2875    2907    3114 
   Size:       1177      32    1005      32     629      32     207

  Fragments arranged by size: 

                1177    1005     629     207      32      32      32
 
HincII GTy'rAC

Cuts at:      0     375     524    2448    2876    3000    3114 
   Size:        375     149    1924     428     124     114

  Fragments arranged by size: 

                1924     428     375     149     124     114
 
HpaI GTT'AAC

Cuts at:      0    2448    3114 
   Size:       2448     666
 
KasI G'GCGC_C

Cuts at:      0    2951    3114 
   Size:       2951     163
 
MfeI C'AATT_G

Cuts at:      0    1943    3114 
   Size:       1943    1171
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     596     765    1299    3114 
   Size:        596     169     534    1815

  Fragments arranged by size: 

                1815     596     534     169
 
MscI TGG'CCA

Cuts at:      0    2186    2498    2633    3114 
   Size:       2186     312     135     481

  Fragments arranged by size: 

                2186     481     312     135
 
MslI CAynn'nnrTG

Cuts at:      0     161     884    3114 
   Size:        161     723    2230

  Fragments arranged by size: 

                2230     723     161
 
MspA1I CmG'CkG

Cuts at:      0      18    1971    3114 
   Size:         18    1953    1143

  Fragments arranged by size: 

                1953    1143      18
 
NarI GG'CG_CC

Cuts at:      0    2952    3114 
   Size:       2952     162
 
NcoI C'CATG_G

Cuts at:      0      43    3080    3114 
   Size:         43    3037      34

  Fragments arranged by size: 

                3037      43      34
 
NspI r_CATG'y

Cuts at:      0    1218    2195    3114 
   Size:       1218     977     919

  Fragments arranged by size: 

                1218     977     919
 
PasI CC'CwG_GG

Cuts at:      0    1096    1273    3114 
   Size:       1096     177    1841

  Fragments arranged by size: 

                1841    1096     177
 
PciI A'CATG_T

Cuts at:      0    1214    3114 
   Size:       1214    1900
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1618    1870    3114 
   Size:       1618     252    1244

  Fragments arranged by size: 

                1618    1244     252
 
PfoI T'CCnGG_A

Cuts at:      0    2843    3114 
   Size:       2843     271
 
PsiI TTA'TAA

Cuts at:      0     974    3114 
   Size:        974    2140
 
PspOMI G'GGCC_C

Cuts at:      0    2525    3114 
   Size:       2525     589
 
PstI C_TGCA'G

Cuts at:      0      17     807    3114 
   Size:         17     790    2307

  Fragments arranged by size: 

                2307     790      17
 
PvuII CAG'CTG

Cuts at:      0    1971    3114 
   Size:       1971    1143
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2793    3114 
   Size:       2793     321
 
ScaI AGT'ACT

Cuts at:      0     841    1869    2103    2489    3114 
   Size:        841    1028     234     386     625

  Fragments arranged by size: 

                1028     841     625     386     234
 
SfcI C'TryA_G

Cuts at:      0      13     803    1733    3114 
   Size:         13     790     930    1381

  Fragments arranged by size: 

                1381     930     790      13
 
SfoI GGC'GCC

Cuts at:      0    2953    3114 
   Size:       2953     161
 
SmlI C'TyrA_G

Cuts at:      0    1179    1426    2029    2941    3114 
   Size:       1179     247     603     912     173

  Fragments arranged by size: 

                1179     912     603     247     173
 
SpeI A'CTAG_T

Cuts at:      0    2104    2266    3114 
   Size:       2104     162     848

  Fragments arranged by size: 

                2104     848     162
 
SspI AAT'ATT

Cuts at:      0     173     823    1695    2577    3114 
   Size:        173     650     872     882     537

  Fragments arranged by size: 

                 882     872     650     537     173
 
StuI AGG'CCT

Cuts at:      0    2811    3114 
   Size:       2811     303
 
StyI C'CwwG_G

Cuts at:      0      43     129     662    3080    3114 
   Size:         43      86     533    2418      34

  Fragments arranged by size: 

                2418     533      86      43      34
 
TatI w'GTAC_w

Cuts at:      0     839    1258    1867    2101    2487    3114 
   Size:        839     419     609     234     386     627

  Fragments arranged by size: 

                 839     627     609     419     386     234
 
XbaI T'CTAG_A

Cuts at:      0     597    3114 
   Size:        597    2517
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2421    2476    2841    2982    3114 
   Size:       2421      55     365     141     132

  Fragments arranged by size: 

                2421     365     141     132      55
 
XmnI GAAnn'nnTTC

Cuts at:      0    2645    2700    3114 
   Size:       2645      55     414

  Fragments arranged by size: 

                2645     414      55
 

 Enzymes that do cut:

     AarI     AccI    AflII   AflIII     AleI    AlwNI     ApaI     ApoI
     AseI     BaeI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
    BglII Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstXI
    BstYI  BstZ17I     BtgI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EarI  Eco57MI EcoO109I     FalI    HaeII    Hin4I   HincII
     HpaI     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NspI     PasI     PciI    PflMI     PfoI     PsiI   PspOMI
     PstI    PvuII     SapI     ScaI     SfcI     SfoI     SmlI     SpeI
     SspI     StuI     StyI     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI     AgeI     AhdI     AlfI
     AloI    ApaLI     AscI    AsiSI     AvaI    AvrII    BamHI     BcgI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI    BsaAI
    BsaWI    BsiEI    BsiWI    BspEI    BsrBI    BsrFI   BssHII    BstBI
   BstEII   Bsu36I    BtgZI     ClaI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     KpnI     MluI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PmeI     PmlI     PpiI    PpuMI    PshAI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SwaI    TaqII
     TstI  Tth111I     XhoI     XmaI     ZraI
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