Restriction map for pF1KB3699
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AcyI        
AflIII      AgeI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AviII       AvrII       BbeI        BbrPI       BbvCI       
BciVI       BclI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstZ17I     ClaI        CpoI        DraI        
DrdI        EagI        EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoRI       EcoRV       FbaI        
FseI        FspI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MluI        MvnI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccIII       1118  TCCGGA               T^CCGGA
AclI         1223  AACGTT               AA^CGTT
AflII        2134  CTTAAG               C^TTAAG
AhdI          229  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          813  CCTGAGG              CC^T[ACGT]AGG
AspEI         229  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1776  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2472  TGGCCA               TGG^CCA
BcgI         1895  CGAAGGAGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2134  CTTAAG               C^TTAAG
BglI         1637  GCCCTCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI         2091  GGTCTC               GGTCTC(1/5)
BsaWI        1118  TCCGGA               [AT]^CCGG[AT]
BseAI        1118  TCCGGA               T^CCGGA
BsmBI        2499  CGTCTC               CGTCTC(1/5)
BspEI        1118  TCCGGA               T^CCGGA
BspMI        1831  ACCTGC               ACCTGC(4/8)
BsrDI        1058  GCAATG               GCAATG(2/0)
BsrFI        1760  GCCGGC               [GA]^CCGG[CT]
BstXI         881  CCAAAGGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        813  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1760  GCCGGC               [GA]^CCGG[CT]
DraIII       1262  CACTAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      229  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        813  CCTGAGG              CC^T[ACGT]AGG
EcoT22I      2301  ATGCAT               ATGCA^T
HaeII        1757  AGCGCC               [GA]GCGC^[CT]
HindIII      2015  AAGCTT               A^AGCTT
MluNI        2472  TGGCCA               TGG^CCA
MroI         1118  TCCGGA               T^CCGGA
MscI         2472  TGGCCA               TGG^CCA
MslI         1849  CACCAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1760  GCCGGC               GCC^GGC
NgoMIV       1760  GCCGGC               G^CCGGC
NsiI         2301  ATGCAT               ATGCA^T
PflFI        1776  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I     1223  AACGTT               AA^CGTT
ScaI           98  AGTACT               AGT^ACT
SmaI         2006  CCCGGG               CCC^GGG
StuI         1530  AGGCCT               AGG^CCT
Tth111I      1776  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2006  CCCGGG               C^CCGGG
XmaI         2006  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

ApaI          371  GGGCCC               GGGCC^C
             1488  GGGCCC              
AvaI         1162  CCCGAG               C^[CT]CG[GA]G
             2006  CCCGGG              
BanI         1786  GGCACC               G^G[CT][GA]CC
             2181  GGTGCC              
BsoBI        1162  CCCGAG               C^[CT]CG[GA]G
             2006  CCCGGG              
BstAPI        252  GCACAATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1826  GCATTACCTGC         
BstEII       1201  GGTTACC              G^GT[ACGT]ACC
             2455  GGTGACC             
BstPI        1201  GGTTACC              G^GT[ACGT]ACC
             2455  GGTGACC             
DsaI          756  CCACGG               C^C[GA][CT]GG
             2483  CCGTGG              
EcoO65I      1201  GGTTACC              G^GT[ACGT]ACC
             2455  GGTGACC             
FauI         1917  CCCGC                CCCGC(4/6)
             2504  CCCGC               
HgaI         1724  GACGC                GACGC(5/10)
              927  GCGTC               
MaeII        1224  ACGT                 A^CGT
             2498  ACGT                
MfeI          376  CAATTG               C^AATTG
             1388  CAATTG              
MunI          376  CAATTG               C^AATTG
             1388  CAATTG              
NspI          968  GCATGC               [GA]CATG^[CT]
             1553  GCATGC              
PflMI         183  CCACTTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              756  CCACGGGTTGG         
PpuMI         227  AGGACCC              [GA]G^G[AT]CC[CT]
             2337  AGGACCT             
SacI          743  GAGCTC               GAGCT^C
             2194  GAGCTC              
SmlI         1000  CTCAAG               C^T[CT][GA]AG
             2134  CTTAAG              
SphI          968  GCATGC               GCATG^C
             1553  GCATGC              
Van91I        183  CCACTTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              756  CCACGGGTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BamHI       
BanII       BbsI        BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
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