Restriction map for pF1KB3683
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AvaI        AviII       AvrII       BanII       
BbeI        BbvCI       BcgI        BfrI        BlnI        
BsaWI       BseAI       BsiEI       BsiWI       BsoBI       
BspDI       BspEI       BspLU11I    BsrBI       BsrFI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Cfr10I      ClaI        CpoI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoT22I     FauI        FseI        FspI        
HaeII       KasI        KpnI        MluI        MroI        
NaeI        NarI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       Psp1406I    PvuI        RsrII       
SacI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    StuI        SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AatII        1772  GACGTC               GACGT^C
AcyI         1772  GACGTC               G[GA]^CG[CT]C
AflIII       2497  ACACGT               A^C[GA][CT]GT
AhdI         2928  GACTATTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        3411  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1758  CCTAAGG              CC^T[ACGT]AGG
AseI          559  ATTAAT               AT^TAAT
AsnI          559  ATTAAT               AT^TAAT
AspEI        2928  GACTATTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2813  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         537  GGATCC               G^GATCC
BanI          736  GGTGCC               G^G[CT][GA]CC
BbrPI        2498  CACGTG               CAC^GTG
BciVI        1096  GGATAC               GTATCC(6/5)
BcnI           54  CCCGG                CC^[GC]GG
BglI         1579  GCCTTGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3232  AGATCT               A^GATCT
BlpI          211  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      211  GCTCAGC              GC^T[ACGT]AGC
BsaHI        1772  GACGTC               G[GA]^CG[CT]C
BsgI         3022  GTGCAG               GTGCAG(16/14)
Bsp1407I     3083  TGTACA               T^GTACA
BspHI        2384  TCATGA               T^CATGA
BspMI        1442  ACCTGC               ACCTGC(4/8)
BsrGI        3083  TGTACA               T^GTACA
Bsu36I       1758  CCTAAGG              CC^T[ACGT]AGG
CelII         211  GCTCAGC              GC^T[ACGT]AGC
CfoI          762  GCGC                 GCG^C
DraI         1452  TTTAAA               TTT^AAA
DraIII       2081  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     2928  GACTATTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1758  CCTAAGG              CC^T[ACGT]AGG
EcoRI         678  GAATTC               G^AATTC
EcoRV        2890  GATATC               GAT^ATC
HapII          55  CCGG                 C^CGG
HhaI          762  GCGC                 GCG^C
Hin1I        1772  GACGTC               G[GA]^CG[CT]C
HinP1I        762  GCGC                 G^CGC
HindIII       920  AAGCTT               A^AGCTT
HpaI         3016  GTTAAC               GTT^AAC
HpaII          55  CCGG                 C^CGG
KspI         2406  CCGCGG               CCGC^GG
MspI           55  CCGG                 C^CGG
NciI           54  CCCGG                CC^[GC]GG
NheI         1482  GCTAGC               G^CTAGC
PflFI        2813  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2785  CCACAGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        2498  CACGTG               CAC^GTG
PmlI         2498  CACGTG               CAC^GTG
PshBI         559  ATTAAT               AT^TAAT
PstI         1673  CTGCAG               CTGCA^G
RcaI         2384  TCATGA               T^CATGA
SacII        2406  CCGCGG               CCGC^GG
SalI         2810  GTCGAC               G^TCGAC
ScaI          697  AGTACT               AGT^ACT
SmlI          673  CTTGAG               C^T[CT][GA]AG
SpeI         2692  ACTAGT               A^CTAGT
SspBI        3083  TGTACA               T^GTACA
SspI         3120  AATATT               AAT^ATT
Tth111I      2813  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2785  CCACAGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1973  CCAGTGGGGTAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AspHI         308  GTGCTC               G[AT]GC[AT]^C
             2643  GAGCAC              
BalI         1970  TGGCCA               TGG^CCA
             2339  TGGCCA              
BbsI          178  GAAGAC               GAAGAC(2/6)
             2371  GAAGAC              
BmyI          308  GTGCTC               G[AGT]GC[ACT]^C
             2643  GAGCAC              
BpuAI         178  GAAGAC               GAAGAC(2/6)
             2371  GAAGAC              
BsaAI         354  CACGTA               [CT]AC^GT[GA]
             2498  CACGTG              
BsaI         1072  GAGACC               GGTCTC(1/5)
             1537  GAGACC              
BsiHKAI       308  GTGCTC               G[AT]GC[AT]^C
             2643  GAGCAC              
BsmFI        3192  GGGAC                GGGAC(10/14)
             2154  GTCCC               
Bsp1286I      308  GTGCTC               G[AGT]GC[ACT]^C
             2643  GAGCAC              
BsrDI        2343  CATTGC               GCAATG(2/0)
             2026  GCAATG              
BssSI        3182  CTCGTG               CACGAG(-5/-1)
             3236  CTCGTG              
BstAPI       1675  GCAGCTATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3410  GCAGGGGCTGC         
DrdI         1419  GACTTTTGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2277  GACTACACGGTC        
EaeI         1970  TGGCCA               [CT]^GGCC[GA]
             2339  TGGCCA              
MfeI         1924  CAATTG               C^AATTG
             2333  CAATTG              
MluNI        1970  TGGCCA               TGG^CCA
             2339  TGGCCA              
MscI         1970  TGGCCA               TGG^CCA
             2339  TGGCCA              
MunI         1924  CAATTG               C^AATTG
             2333  CAATTG              
NcoI         3221  CCATGG               C^CATGG
             3288  CCATGG              
NdeI         3157  CATATG               CA^TATG
             3401  CATATG              
PvuII         127  CAGCTG               CAG^CTG
             2396  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        ApoI        Asp700I     AvaII       
BbvI        BclI        BfaI        BpmI        BsaBI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmBI       BsmI        BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     FbaI        Fnu4HI      
FokI        HaeIII      HgaI        HincII      HindII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SphI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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