Restriction map for pF1KB3674
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        Alw44I      
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmI        BsoBI       BspDI       BspEI       BspHI       
BsrBI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
EclXI       Eco52I      Eco81I      EcoNI       EcoRI       
EcoT14I     EcoT22I     FauI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
StyI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI         1348  AAATTT               [GA]^AATT[CT]
AflIII       1270  ACATGT               A^C[GA][CT]GT
AhdI          931  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       456  AGCGCT               AGC^GCT
ApoI         1348  AAATTT               [GA]^AATT[CT]
AspEI         931  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          818  TGGCCA               TGG^CCA
BanI          255  GGCACC               G^G[CT][GA]CC
BanII         884  GGGCTC               G[GA]GC[CT]^C
BclI         1019  TGATCA               T^GATCA
BlpI         1504  GCTCAGC              GC^T[ACGT]AGC
BpmI          887  CTCCAG               CTGGAG(16/14)
Bpu1102I     1504  GCTCAGC              GC^T[ACGT]AGC
BsaI          492  GGTCTC               GGTCTC(1/5)
BsgI           91  CTGCAC               GTGCAG(16/14)
BsmBI         654  CGTCTC               CGTCTC(1/5)
BsmFI         998  GTCCC                GGGAC(10/14)
BspLU11I     1270  ACATGT               A^CATGT
BsrFI         252  ACCGGC               [GA]^CCGG[CT]
BstAPI        193  GCAGCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII         40  GGTCACC              G^GT[ACGT]ACC
BstPI          40  GGTCACC              G^GT[ACGT]ACC
BstXI         729  CCATTCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1504  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        252  ACCGGC               [GA]^CCGG[CT]
DraII        1098  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EaeI          818  TGGCCA               [CT]^GGCC[GA]
Eam1105I      931  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      456  AGCGCT               AGC^GCT
EcoO109I     1098  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I        40  GGTCACC              G^GT[ACGT]ACC
EcoRV        1262  GATATC               GAT^ATC
FbaI         1019  TGATCA               T^GATCA
HaeIII        819  GGCC                 GG^CC
MaeII         476  ACGT                 A^CGT
MluNI         818  TGGCCA               TGG^CCA
MscI          818  TGGCCA               TGG^CCA
NdeI          850  CATATG               CA^TATG
NspI         1270  ACATGT               [GA]CATG^[CT]
PpuMI        1098  GGGTCCT              [GA]G^G[AT]CC[CT]
SspI          978  AATATT               AAT^ATT
XcmI         1001  CCATTCAGCAGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Asp700I      1159  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1399  GAAATTATTC          
AvaII         274  GGACC                G^G[AT]CC
             1099  GGTCC               
BbsI          478  GTCTTC               GAAGAC(2/6)
              508  GTCTTC              
BmyI          884  GGGCTC               G[AGT]GC[ACT]^C
             1035  GGGCAC              
BpuAI         478  GTCTTC               GAAGAC(2/6)
              508  GTCTTC              
Bsp1286I      884  GGGCTC               G[AGT]GC[ACT]^C
             1035  GGGCAC              
Bsp1407I      144  TGTACA               T^GTACA
              153  TGTACA              
BspMI          89  ACCTGC               ACCTGC(4/8)
               37  GCAGGT              
BsrDI         920  GCAATG               GCAATG(2/0)
             1054  GCAATG              
BsrGI         144  TGTACA               T^GTACA
              153  TGTACA              
BstYI         432  AGATCC               [GA]^GATC[CT]
             1026  GGATCT              
Cac8I         264  GCAGGC               GC[ACGT]^[ACGT]GC
              736  GCTGGC              
Cfr13I        274  GGACC                G^G[ACGT]CC
             1099  GGTCC               
HaeII         456  AGCGCT               [GA]GCGC^[CT]
             1075  GGCGCT              
MflI          432  AGATCC               [GA]^GATC[CT]
             1026  GGATCT              
PflMI         436  CCAAGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              483  CCAGAATCTGG         
PleI          855  GACTC                GAGTC(4/5)
              996  GAGTC               
Sau96I        274  GGACC                G^G[ACGT]CC
             1099  GGTCC               
SspBI         144  TGTACA               T^GTACA
              153  TGTACA              
TaqI          750  TCGA                 T^CGA
             1364  TCGA                
TthHB8I       750  TCGA                 T^CGA
             1364  TCGA                
Van91I        436  CCAAGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              483  CCAGAATCTGG         
XhoII         432  AGATCC               [GA]^GATC[CT]
             1026  GGATCT              
XmnI         1159  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1399  GAAATTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
BbvI        BcnI        BfaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsrI        BssKI       
BstF5I      BstNI       CfoI        DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PstI        PvuII       RsaI        
Sau3AI      ScrFI       SfaNI       SfcI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
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