Restriction map for pF1KB3674
 (Linear) (Six-base) MAPSORT of: KIBB3674.seq  Check: 7670  from: 1  to: 1545



KIBB3674.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      98    1545 
   Size:         98    1447
 
AfeI AGC'GCT

Cuts at:      0     458    1545 
   Size:        458    1087
 
AflIII A'CryG_T

Cuts at:      0    1270    1545 
   Size:       1270     275
 
AhdI GACnn_n'nnGTC

Cuts at:      0     936    1545 
   Size:        936     609
 
AlwNI CAG_nnn'CTG

Cuts at:      0     199     369     489     788    1545 
   Size:        199     170     120     299     757

  Fragments arranged by size: 

                 757     299     199     170     120
 
ApoI r'AATT_y

Cuts at:      0    1348    1545 
   Size:       1348     197
 
BanI G'GyrC_C

Cuts at:      0     255    1545 
   Size:        255    1290
 
BanII G_rGCy'C

Cuts at:      0     888    1545 
   Size:        888     657
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     471     501    1545 
   Size:        471      30    1044

  Fragments arranged by size: 

                1044     471      30
 
BclI T'GATC_A

Cuts at:      0    1019    1545 
   Size:       1019     526
 
BlpI GC'TnA_GC

Cuts at:      0    1505    1545 
   Size:       1505      40
 
Bme1580I G_kGCm'C

Cuts at:      0    1039    1545 
   Size:       1039     506
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     475    1465    1545 
   Size:        475     990      80

  Fragments arranged by size: 

                 990     475      80
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     872    1545 
   Size:        872     673
 
Bpu10I CC'TnA_GC

Cuts at:      0     296    1079    1217    1227    1545 
   Size:        296     783     138      10     318

  Fragments arranged by size: 

                 783     318     296     138      10
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     498    1545 
   Size:        498    1047
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      71      74     650    1520    1545 
   Size:         71       3     576     870      25

  Fragments arranged by size: 

                 870     576      71      25       3
 
BseYI C'CCAG_C

Cuts at:      0     291    1545 
   Size:        291    1254
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76    1545 
   Size:         76    1469
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     660    1545 
   Size:        660     885
 
Bsp1286I G_dGCh'C

Cuts at:      0     888    1039    1545 
   Size:        888     151     506

  Fragments arranged by size: 

                 888     506     151
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      28      98    1545 
   Size:         28      70    1447

  Fragments arranged by size: 

                1447      70      28
 
BsrDI GCAATG_nn'

Cuts at:      0     927    1061    1545 
   Size:        927     134     484

  Fragments arranged by size: 

                 927     484     134
 
BsrFI r'CCGG_y

Cuts at:      0     252    1545 
   Size:        252    1293
 
BsrGI T'GTAC_A

Cuts at:      0     144     153    1545 
   Size:        144       9    1392

  Fragments arranged by size: 

                1392     144       9
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     199    1545 
   Size:        199    1346
 
BstEII G'GTnAC_C

Cuts at:      0      40    1545 
   Size:         40    1505
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     736    1545 
   Size:        736     809
 
BstYI r'GATC_y

Cuts at:      0     432    1026    1545 
   Size:        432     594     519

  Fragments arranged by size: 

                 594     519     432
 
EaeI y'GGCC_r

Cuts at:      0     818    1545 
   Size:        818     727
 
EarI CTCTTCn'nnn_

Cuts at:      0     174    1124    1302    1545 
   Size:        174     950     178     243

  Fragments arranged by size: 

                 950     243     178     174
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     872    1545 
   Size:        872     673
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1099    1545 
   Size:       1099     446
 
EcoRV GAT'ATC

Cuts at:      0    1264    1545 
   Size:       1264     281
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     712    1242    1545 
   Size:        712     530     303

  Fragments arranged by size: 

                 712     530     303
 
HaeII r_GCGC'y

Cuts at:      0     460    1079    1545 
   Size:        460     619     466

  Fragments arranged by size: 

                 619     466     460
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     922     954    1011    1043    1545 
   Size:        922      32      57      32     502

  Fragments arranged by size: 

                 922     502      57      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     600     992    1410    1545 
   Size:        600     392     418     135

  Fragments arranged by size: 

                 600     418     392     135
 
MscI TGG'CCA

Cuts at:      0     820    1545 
   Size:        820     725
 
MslI CAynn'nnrTG

Cuts at:      0     548    1273    1275    1545 
   Size:        548     725       2     270

  Fragments arranged by size: 

                 725     548     270       2
 
MspA1I CmG'CkG

Cuts at:      0     142     178     196     205     280     316     369     736 
   Size:        142      36      18       9      75      36      53     367

Cuts at:    736    1545 
   Size:        809

  Fragments arranged by size: 

                 809     367     142      75      53      36      36      18
                   9
 
NdeI CA'TA_TG

Cuts at:      0     851    1545 
   Size:        851     694
 
NspI r_CATG'y

Cuts at:      0    1274    1545 
   Size:       1274     271
 
PasI CC'CwG_GG

Cuts at:      0     223    1545 
   Size:        223    1322
 
PciI A'CATG_T

Cuts at:      0    1270    1545 
   Size:       1270     275
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     442     489    1545 
   Size:        442      47    1056

  Fragments arranged by size: 

                1056     442      47
 
PfoI T'CCnGG_A

Cuts at:      0     270     512    1545 
   Size:        270     242    1033

  Fragments arranged by size: 

                1033     270     242
 
PpuMI rG'GwC_Cy

Cuts at:      0    1099    1545 
   Size:       1099     446
 
PstI C_TGCA'G

Cuts at:      0      30     204     210     285    1545 
   Size:         30     174       6      75    1260

  Fragments arranged by size: 

                1260     174      75      30       6
 
PvuII CAG'CTG

Cuts at:      0     178     205     280     316     369     736    1545 
   Size:        178      27      75      36      53     367     809

  Fragments arranged by size: 

                 809     367     178      75      53      36      27
 
SfcI C'TryA_G

Cuts at:      0      26     200     206     281     320    1545 
   Size:         26     174       6      75      39    1225

  Fragments arranged by size: 

                1225     174      75      39      26       6
 
SspI AAT'ATT

Cuts at:      0     980    1545 
   Size:        980     565
 
TatI w'GTAC_w

Cuts at:      0     144     153     552    1125    1545 
   Size:        144       9     399     573     420

  Fragments arranged by size: 

                 573     420     399     144       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1053    1085    1427    1459    1545 
   Size:       1053      32     342      32      86

  Fragments arranged by size: 

                1053     342      86      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1008    1545 
   Size:       1008     537
 
XmnI GAAnn'nnTTC

Cuts at:      0    1163    1403    1545 
   Size:       1163     240     142

  Fragments arranged by size: 

                1163     240     142
 

 Enzymes that do cut:

     AarI     AfeI   AflIII     AhdI    AlwNI     ApoI     BanI    BanII
     BbsI     BclI     BlpI Bme1580I     BplI     BpmI   Bpu10I     BsaI
    BseRI    BseYI     BsgI    BsmBI Bsp1286I    BspMI    BsrDI    BsrFI
    BsrGI   BstAPI   BstEII    BstXI    BstYI     EaeI     EarI  Eco57MI
 EcoO109I    EcoRV     FalI    HaeII    Hin4I     MmeI     MscI     MslI
   MspA1I     NdeI     NspI     PasI     PciI    PflMI     PfoI    PpuMI
     PstI    PvuII     SfcI     SspI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII    BmgBI     BmrI     BmtI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI
    BspHI    BsrBI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmaI     ZraI
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