Restriction map for pF1KB3596
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       AccI        AccIII      AcyI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
AspEI       AviII       AvrII       BalI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspLU11I    BspMI       BsrFI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HindIII     HpaI        KasI        
KspI        MamI        MluI        MluNI       MroI        
MscI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PstI        PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

Acc65I        964  GGTACC               G^GTACC
AccII          23  CGCG                 CG^CG
AclI          716  AACGTT               AA^CGTT
AcsI         1256  GAATTC               [GA]^AATT[CT]
Alw44I        257  GTGCAC               G^TGCAC
ApaLI         257  GTGCAC               G^TGCAC
ApoI         1256  GAATTC               [GA]^AATT[CT]
Asp718I       964  GGTACC               G^GTACC
AspHI         257  GTGCAC               G[AT]GC[AT]^C
AspI          552  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         856  GGATCC               G^GATCC
BanI          964  GGTACC               G^G[CT][GA]CC
BanII         154  GGGCTC               G[GA]GC[CT]^C
BsaI          178  GAGACC               GGTCTC(1/5)
BsiEI          11  CGGTCG               CG[GA][CT]^CG
BsiHKAI       257  GTGCAC               G[AT]GC[AT]^C
BsmFI          26  GTCCC                GGGAC(10/14)
Bsp1407I      121  TGTACA               T^GTACA
BsrBI         930  GAGCGG               CCGCTC(-3/-3)
BsrGI         121  TGTACA               T^GTACA
BssSI          43  CTCGTG               CACGAG(-5/-1)
BstF5I        774  CATCC                GGATG(2/0)
BstUI          23  CGCG                 CG^CG
BstXI        1363  CCAAACAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         856  GGATCC               [GA]^GATC[CT]
CfoI           76  GCGC                 GCG^C
DraII         485  AGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       1111  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          923  CCACGG               C^C[GA][CT]GG
EcoO109I      485  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1256  GAATTC               G^AATTC
FauI          672  GCGGG                CCCGC(4/6)
FokI          774  CATCC                GGATG(9/13)
HaeII          75  GGCGCT               [GA]GCGC^[CT]
HgaI           24  GCGTC                GACGC(5/10)
HhaI           76  GCGC                 GCG^C
HinP1I         76  GCGC                 G^CGC
HincII       1487  GTCAAC               GT[CT]^[GA]AC
HindII       1487  GTCAAC               GT[CT]^[GA]AC
KpnI          964  GGTACC               GGTAC^C
MfeI          599  CAATTG               C^AATTG
MflI          856  GGATCC               [GA]^GATC[CT]
MspA1I        380  CAGCTG               C[AC]G^C[GT]G
MunI          599  CAATTG               C^AATTG
MvnI           23  CGCG                 CG^CG
NspI          206  GCATGT               [GA]CATG^[CT]
PaeR7I        236  CTCGAG               C^TCGAG
PflFI         552  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1110  CCACAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          819  GACTC                GAGTC(4/5)
PpuMI         485  AGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I      716  AACGTT               AA^CGTT
PvuII         380  CAGCTG               CAG^CTG
SmaI           84  CCCGGG               CCC^GGG
SspBI         121  TGTACA               T^GTACA
Tth111I       552  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1110  CCACAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          706  TCTAGA               T^CTAGA
XhoI          236  CTCGAG               C^TCGAG
XhoII         856  GGATCC               [GA]^GATC[CT]
XmaCI          84  CCCGGG               C^CCGGG
XmaI           84  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI          857  GATCC                GGATC(4/5)
              856  GGATC               
AlwNI         560  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              887  CAGTTACTG           
AvaI           84  CCCGGG               C^[CT]CG[GA]G
              236  CTCGAG              
BfaI          707  CTAG                 C^TAG
             1442  CTAG                
BmyI          154  GGGCTC               G[AGT]GC[ACT]^C
              257  GTGCAC              
BsmAI         178  GAGAC                GTCTC(1/5)
              166  GTCTC               
BsoBI          84  CCCGGG               C^[CT]CG[GA]G
              236  CTCGAG              
Bsp1286I      154  GGGCTC               G[AGT]GC[ACT]^C
              257  GTGCAC              
BstNI         355  CCTGG                CC^[AT]GG
              777  CCTGG               
EarI          264  CTCTTC               CTCTTC(1/4)
              481  GAAGAG              
EcoRII        355  CCTGG                ^CC[AT]GG
              777  CCTGG               
EcoT14I       360  CCTTGG               C^C[AT][AT]GG
              969  CCAAGG              
HapII          30  CCGG                 C^CGG
               85  CCGG                
HpaII          30  CCGG                 C^CGG
               85  CCGG                
HphI          352  TCACC                GGTGA(8/7)
              462  TCACC               
Ksp632I       264  CTCTTC               CTCTTC(1/4)
              481  GAAGAG              
MaeI          707  CTAG                 C^TAG
             1442  CTAG                
MspI           30  CCGG                 C^CGG
               85  CCGG                
MvaI          355  CCTGG                CC^[AT]GG
              777  CCTGG               
SfaNI         413  GCATC                GCATC(5/9)
             1499  GCATC               
SmlI          236  CTCGAG               C^T[CT][GA]AG
             1389  CTTGAG              
StyI          360  CCTTGG               C^C[AT][AT]GG
              969  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BcnI        BsaJI       BseRI       BsiYI       BslI        
BspHI       BsrDI       BsrI        BssKI       Cac8I       
Cfr13I      DdeI        DpnII       Fnu4HI      HaeIII      
HinfI       ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MwoI        
NciI        NdeII       NlaIII      NlaIV       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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