Restriction map for pF1KB3596
 (Circular) (Six-base) MAPSORT of: pF1KB3596.seq  Check: 3268  from: 1  to: 4622



pF1KB3596  4622 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AccI GT'mk_AC

Cuts at:   1660    1660 
   Size:       4622
 
Acc65I G'GTAC_C

Cuts at:   1072    1638    1072 
   Size:        566    4056
 
AclI AA'CG_TT

Cuts at:    825    4462     825 
   Size:       3637     985
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1271    2447    2879    3232    3894    1271 
   Size:       1176     432     353     662    1999

  Fragments arranged by size: 

                1999    1176     662     432     353
 
AflIII A'CryG_T

Cuts at:   1974    3774    4055    1974 
   Size:       1800     281    2541

  Fragments arranged by size: 

                2541    1800     281
 
AlwNI CAG_nnn'CTG

Cuts at:    673    1000    1927    3365     673 
   Size:        327     927    1438    1930

  Fragments arranged by size: 

                1930    1438     927     327
 
ApaLI G'TGCA_C

Cuts at:    365    3460     365 
   Size:       3095    1527
 
ApoI r'AATT_y

Cuts at:   1364    1626    3975    4231    1364 
   Size:        262    2349     256    1755

  Fragments arranged by size: 

                2349    1755     262     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4622
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4622
 
AvaI C'yCGr_G

Cuts at:    192     344    1642     192 
   Size:        152    1298    3172

  Fragments arranged by size: 

                3172    1298     152
 
BamHI G'GATC_C

Cuts at:    964    1647     964 
   Size:        683    3939
 
BanI G'GyrC_C

Cuts at:   1072    1638    2052    2300    2335    1072 
   Size:        566     414     248      35    3359

  Fragments arranged by size: 

                3359     566     414     248      35
 
BanII G_rGCy'C

Cuts at:    266    1636    2666     266 
   Size:       1370    1030    2222

  Fragments arranged by size: 

                2222    1370    1030
 
BbeI G_GCGC'C

Cuts at:   2056    2304    2056 
   Size:        248    4374
 
BciVI GTATCCnnnnn_n'

Cuts at:   2520    3576    4622    2520 
   Size:       1056    1046    2520

  Fragments arranged by size: 

                2520    1056    1046
 
BclI T'GATC_A

Cuts at:   2142    2142 
   Size:       4622
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       4622
 
BglII A'GATC_T

Cuts at:   2137    4174    2137 
   Size:       2037    2585
 
BlpI GC'TnA_GC

Cuts at:   1734    1734 
   Size:       4622
 
Bme1580I G_kGCm'C

Cuts at:    369    2247    2340    3464     369 
   Size:       1878      93    1124    1527

  Fragments arranged by size: 

                1878    1527    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    392    1834    1915    2250    4394     392 
   Size:       1442      81     335    2144     620

  Fragments arranged by size: 

                2144    1442     620     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2989    2989 
   Size:       4622
 
Bpu10I CC'TnA_GC

Cuts at:    678     678 
   Size:       4622
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1518    1798    2677    3391    3689    1518 
   Size:        280     879     714     298    2451

  Fragments arranged by size: 

                2451     879     714     298     280
 
BsaI GGTCTCn'nnnn_

Cuts at:    280    4326     280 
   Size:       4046     576
 
BsaAI yAC'GTr

Cuts at:   1975    1975 
   Size:       4622
 
BsaBI GATnn'nnATC

Cuts at:      6    2136    4173       6 
   Size:       2130    2037     455

  Fragments arranged by size: 

                2130    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2053    2301    3003    4499    2053 
   Size:        248     702    1496    2176

  Fragments arranged by size: 

                2176    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1811    2332    3010    3421    3568    3842    1811 
   Size:        521     678     411     147     274    2591

  Fragments arranged by size: 

                2591     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    776     806     776 
   Size:         30    4592
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    136     139     801     136 
   Size:          3     662    3957

  Fragments arranged by size: 

                3957     662       3
 
BseYI C'CCAG_C

Cuts at:   2047    2805    3470    3878    2047 
   Size:        758     665     408    2791

  Fragments arranged by size: 

                2791     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104     122    2164    2210    3440     104 
   Size:         18    2042      46    1230    1286

  Fragments arranged by size: 

                2042    1286    1230      46      18
 
BsiHKAI G_wGCw'C

Cuts at:    369    1636    2414    2604    3464     369 
   Size:       1267     778     190     860    1527

  Fragments arranged by size: 

                1527    1267     860     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    266     369    1636    2247    2340    2414    2604    2666    3464 
   Size:        103    1267     611      93      74     190      62     798

Cuts at:   3464     266 
   Size:       1424

  Fragments arranged by size: 

                1424    1267     798     611     190     103      93      74
                  62
 
BspHI T'CATG_A

Cuts at:    873     954    1368    3054     873 
   Size:         81     414    1686    2441

  Fragments arranged by size: 

                2441    1686     414      81
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1672    1886    2188    2569    1672 
   Size:        214     302     381    3725

  Fragments arranged by size: 

                3725     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1040    2914    2968    4452    4617      50 
   Size:        990    1874      54    1484     165      55

  Fragments arranged by size: 

                1874    1484     990     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    420     494     688    1091    1322    1561    2534     420 
   Size:         74     194     403     231     239     973    2508

  Fragments arranged by size: 

                2508     973     403     239     231     194      74
 
BsrFI r'CCGG_y

Cuts at:   2620    2801    2620 
   Size:        181    4441
 
BsrGI T'GTAC_A

Cuts at:    229     229 
   Size:       4622
 
BssSI C'ACGA_G

Cuts at:    151    2893    3601     151 
   Size:       2742     708    1172

  Fragments arranged by size: 

                2742    1172     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1999    1999 
   Size:       4622
 
BstBI TT'CG_AA

Cuts at:   2983    2983 
   Size:       4622
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1478    1478 
   Size:       4622
 
BstYI r'GATC_y

Cuts at:      1     964    1647    2114    2137    2472    2718    3024    3036 
   Size:        963     683     467      23     335     246     306      12

Cuts at:   3036    3122    4174       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1052     963     683     467     449     335     306     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107    1031     107 
   Size:        924    3698
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2572    2753    2916    2572 
   Size:        181     163    4278

  Fragments arranged by size: 

                4278     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1865    1865 
   Size:       4622
 
DraI TTT'AAA

Cuts at:   1621    1621 
   Size:       4622
 
DraIII CAC_nnn'GTG

Cuts at:   1224    1224 
   Size:       4622
 
DrdI GACnn_nn'nnGTC

Cuts at:   2328    3672    2328 
   Size:       1344    3278
 
EaeI y'GGCC_r

Cuts at:   2207    2381    2772    2799    4144    2207 
   Size:        174     391      27    1345    2685

  Fragments arranged by size: 

                2685    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2207    2207 
   Size:       4622
 
EarI CTCTTCn'nnn_

Cuts at:    378     584    2645    2855     378 
   Size:        206    2061     210    2145

  Fragments arranged by size: 

                2145    2061     210     206
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2830    3572    3718    4432    2830 
   Size:        742     146     714    3020

  Fragments arranged by size: 

                3020     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1634    1634 
   Size:       4622
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1271    2447    2879    3232    3894    1271 
   Size:       1176     432     353     662    1999

  Fragments arranged by size: 

                1999    1176     662     432     353
 
EcoO109I rG'GnC_Cy

Cuts at:    594    1761     594 
   Size:       1167    3455
 
EcoRI G'AATT_C

Cuts at:   1364    1626    1364 
   Size:        262    4360
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1140    2641    1140 
   Size:       1501    3121
 
FspI TGC'GCA

Cuts at:   2403    2403 
   Size:       4622
 
HaeII r_GCGC'y

Cuts at:    187    2056    2304    3534    4305     187 
   Size:       1869     248    1230     771     504

  Fragments arranged by size: 

                1869    1230     771     504     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    949     981    1096    1128    2464    2494    2496    2526     949 
   Size:         32     115      32    1336      30       2      30    3045

  Fragments arranged by size: 

                3045    1336     115      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1597    1661    1597 
   Size:         64    4558
 
KasI G'GCGC_C

Cuts at:   2052    2300    2052 
   Size:        248    4374
 
KpnI G_GTAC'C

Cuts at:   1076    1642    1076 
   Size:        566    4056
 
MfeI C'AATT_G

Cuts at:    707     707 
   Size:       4622
 
MluI A'CGCG_T

Cuts at:   4055    4055 
   Size:       4622
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    466    3381    3565     466 
   Size:       2915     184    1523

  Fragments arranged by size: 

                2915    1523     184
 
MscI TGG'CCA

Cuts at:   2383    2383 
   Size:       4622
 
MslI CAynn'nnrTG

Cuts at:    535     959    1273    1612    2611    2738     535 
   Size:        424     314     339     999     127    2419

  Fragments arranged by size: 

                2419     999     424     339     314     127
 
MspA1I CmG'CkG

Cuts at:    490    1733    2047    2407    3191    3436     490 
   Size:       1243     314     360     784     245    1676

  Fragments arranged by size: 

                1676    1243     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   2803    2803 
   Size:       4622
 
NarI GG'CG_CC

Cuts at:   2053    2301    2053 
   Size:        248    4374
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4622
 
NgoMIV G'CCGG_C

Cuts at:   2801    2801 
   Size:       4622
 
NspI r_CATG'y

Cuts at:    318    1675    2175    3778     318 
   Size:       1357     500    1603    1162

  Fragments arranged by size: 

                1603    1357    1162     500
 
PciI A'CATG_T

Cuts at:   3774    3774 
   Size:       4622
 
PflMI CCAn_nnn'nTGG

Cuts at:   1224    1224 
   Size:       4622
 
PmeI GTTT'AAAC

Cuts at:   1621    1621 
   Size:       4622
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3068    3100    3068 
   Size:         32    4590
 
PpuMI rG'GwC_Cy

Cuts at:    594     594 
   Size:       4622
 
PstI C_TGCA'G

Cuts at:   1669    2354    1669 
   Size:        685    3937
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4622
 
PvuII CAG'CTG

Cuts at:    490    2047    2407     490 
   Size:       1557     360    2705

  Fragments arranged by size: 

                2705    1557     360
 
RsrII CG'GwC_CG

Cuts at:   2817    2817 
   Size:       4622
 
SacI G_AGCT'C

Cuts at:   1636    1636 
   Size:       4622
 
SalI G'TCGA_C

Cuts at:   1659    1659 
   Size:       4622
 
SapI GCTCTTCn'nnn_

Cuts at:   2645    2855    2645 
   Size:        210    4412
 
SbfI CC_TGCA'GG

Cuts at:   1669    1669 
   Size:       4622
 
ScaI AGT'ACT

Cuts at:   4139    4139 
   Size:       4622
 
SfcI C'TryA_G

Cuts at:     33    1665    2350    3318    3509      33 
   Size:       1632     685     968     191    1146

  Fragments arranged by size: 

                1632    1146     968     685     191
 
SfoI GGC'GCC

Cuts at:   2054    2302    2054 
   Size:        248    4374
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4622
 
SmaI CCC'GGG

Cuts at:    194    1644     194 
   Size:       1450    3172
 
SmlI C'TyrA_G

Cuts at:    344    1497    1777    2692    3406    3668     344 
   Size:       1153     280     915     714     262    1298

  Fragments arranged by size: 

                1298    1153     915     714     280     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4622
 
SphI G_CATG'C

Cuts at:   1675    2175    1675 
   Size:        500    4122
 
StyI C'CwwG_G

Cuts at:    107     468    1077    1756    2110     107 
   Size:        361     609     679     354    2619

  Fragments arranged by size: 

                2619     679     609     361     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2205    2338    2797    3006    2205 
   Size:        133     459     209    3821

  Fragments arranged by size: 

                3821     459     209     133
 
TatI w'GTAC_w

Cuts at:    229    4137     229 
   Size:       3908     714
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4139    4171    4139 
   Size:         32    4590
 
Tth111I GACn'n_nGTC

Cuts at:    663    2419     663 
   Size:       1756    2866
 
XbaI T'CTAG_A

Cuts at:    814    1653     814 
   Size:        839    3783
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2117    2117 
   Size:       4622
 
XhoI C'TCGA_G

Cuts at:    344     344 
   Size:       4622
 
XmaI C'CCGG_G

Cuts at:    192    1642     192 
   Size:       1450    3172
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
    PflMI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI
    BfrBI    BmgBI     BmtI     BpmI     BsgI    BsiWI     BsmI    BsmBI
    BspEI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI    CspCI    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XmnI     ZraI
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