Restriction map for pF1KB3499
##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AclI        AflII       AgeI        Aor51HI     AscI        
AseI        AsnI        AspI        AviII       BbrPI       
BciVI       BfrI        BlpI        Bpu1102I    BsiWI       
Bsp1407I    BspDI       BspHI       BsrGI       BstBI       
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoRV       FseI        FspI        HindIII     HpaI        
MluI        NheI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SbfI        ScaI        SfiI        
SfuI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII          12  GACGTC               GACGT^C
Acc65I        909  GGTACC               G^GTACC
AccIII       2027  TCCGGA               T^CCGGA
AhdI         2311  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      1848  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       909  GGTACC               G^GTACC
AspEI        2311  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        2838  CCTAGG               C^CTAGG
BbeI          804  GGCGCC               GGCGC^C
BclI         2772  TGATCA               T^GATCA
BglII        2570  AGATCT               A^GATCT
BlnI         2838  CCTAGG               C^CTAGG
BsaBI         255  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2027  TCCGGA               T^CCGGA
BsiEI        1046  CGACCG               CG[GA][CT]^CG
BspEI        2027  TCCGGA               T^CCGGA
BspLU11I     2355  ACATGT               A^CATGT
BsrDI        2955  GCAATG               GCAATG(2/0)
BssSI        1780  CACGAG               CACGAG(-5/-1)
Bst1107I      433  GTATAC               GTA^TAC
BstAPI       1967  GCATCCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I       433  GTATAC               GTA^TAC
Eam1105I     2311  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2152  GAATTC               G^AATTC
EcoT22I      3200  ATGCAT               ATGCA^T
FbaI         2772  TGATCA               T^GATCA
KasI          804  GGCGCC               G^GCGCC
KpnI          909  GGTACC               GGTAC^C
MamI          255  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2756  CAATTG               C^AATTG
MroI         2027  TCCGGA               T^CCGGA
MunI         2756  CAATTG               C^AATTG
NarI          804  GGCGCC               GG^CGCC
NdeI         1520  CATATG               CA^TATG
NsiI         3200  ATGCAT               ATGCA^T
NspI         2355  ACATGT               [GA]CATG^[CT]
PflMI        2901  CCACCGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1011  GACCTCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          490  GAGCTC               GAGCT^C
SalI          826  GTCGAC               G^TCGAC
SapI         2877  GCTCTTC              GCTCTTC(1/4)
SexAI        2105  ACCAGGT              A^CC[AT]GGT
SfoI          804  GGCGCC               GGC^GCC
SgrAI         738  CGCCGGCG             C[GA]^CCGG[CT]G
StuI         2042  AGGCCT               AGG^CCT
Van91I       2901  CCACCGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1848  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AflIII       1181  ACGTGT               A^C[GA][CT]GT
             2355  ACATGT              
Alw44I        726  GTGCAC               G^TGCAC
              733  GTGCAC              
AlwNI        1916  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2587  CAGTATCTG           
ApaLI         726  GTGCAC               G^TGCAC
              733  GTGCAC              
BcgI         2297  CGAGTGAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1904  GCACGGTGATCG        
BsaAI         222  CACGTA               [CT]AC^GT[GA]
             2713  TACGTG              
BsmBI        1941  CGTCTC               CGTCTC(1/5)
             1569  GAGACG              
BsmI          524  GCATTC               GAATGC(1/-1)
             1326  GCATTC              
BssHII        743  GCGCGC               G^CGCGC
              745  GCGCGC              
BstEII        777  GGTGACC              G^GT[ACGT]ACC
             1362  GGTCACC             
BstPI         777  GGTGACC              G^GT[ACGT]ACC
             1362  GGTCACC             
EcoO65I       777  GGTGACC              G^GT[ACGT]ACC
             1362  GGTCACC             
HaeII         804  GGCGCC               [GA]GCGC^[CT]
              838  AGCGCC              
HincII        826  GTCGAC               GT[CT]^[GA]AC
             2344  GTCAAC              
HindII        826  GTCGAC               GT[CT]^[GA]AC
             2344  GTCAAC              
NcoI         2096  CCATGG               C^CATGG
             2540  CCATGG              
PaeR7I         93  CTCGAG               C^TCGAG
             3146  CTCGAG              
XhoI           93  CTCGAG               C^TCGAG
             3146  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AocI        ApaI        
ApoI        AspHI       AvaI        AvaII       BalI        
BamHI       BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       PleI        PstI        
PvuII       RsaI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmaCI       XmaI        
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