Restriction map for pF1KB3481
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AccI        AgeI        AhdI        
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BamHI       BbrPI       BcgI        
BglI        BlnI        BsaAI       BsaBI       BsiEI       
BsiWI       BspDI       BsrDI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     ClaI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FseI        FspI        HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshBI       PvuI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        StuI        Van91I      XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccIII        890  TCCGGA               T^CCGGA
AclI          845  AACGTT               AA^CGTT
AflII         594  CTTAAG               C^TTAAG
AflIII       2313  ACATGT               A^C[GA][CT]GT
Aor51HI      2333  AGCGCT               AGC^GCT
ApaI          685  GGGCCC               GGGCC^C
Asp700I       106  GAAGCGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          798  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          243  TGGCCA               TGG^CCA
BbeI         2301  GGCGCC               GGCGC^C
BbsI         2095  GAAGAC               GAAGAC(2/6)
BbvCI        1522  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1987  GTATCC               GTATCC(6/5)
BfaI         1918  CTAG                 C^TAG
BfrI          594  CTTAAG               C^TTAAG
BlpI          426  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      426  GCTCAGC              GC^T[ACGT]AGC
BpuAI        2095  GAAGAC               GAAGAC(2/6)
BsaI           11  GAGACC               GGTCTC(1/5)
BsaWI         890  TCCGGA               [AT]^CCGG[AT]
BseAI         890  TCCGGA               T^CCGGA
BsgI         1372  GTGCAG               GTGCAG(16/14)
BsmBI        1751  CGTCTC               CGTCTC(1/5)
BsmI          586  GAATGC               GAATGC(1/-1)
BspEI         890  TCCGGA               T^CCGGA
BspLU11I     2313  ACATGT               A^CATGT
BspMI         132  GCAGGT               ACCTGC(4/8)
BsrFI        1169  GCCGGT               [GA]^CCGG[CT]
CelII         426  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1169  GCCGGT               [GA]^CCGG[CT]
CpoI           20  CGGACCG              CG^G[AT]CCG
DraI         1437  TTTAAA               TTT^AAA
EaeI          243  TGGCCA               [CT]^GGCC[GA]
Eco47III     2333  AGCGCT               AGC^GCT
EcoRV         913  GATATC               GAT^ATC
HincII        904  GTCAAC               GT[CT]^[GA]AC
HindII        904  GTCAAC               GT[CT]^[GA]AC
KasI         2301  GGCGCC               G^GCGCC
MaeI         1918  CTAG                 C^TAG
MluNI         243  TGGCCA               TGG^CCA
MroI          890  TCCGGA               T^CCGGA
MscI          243  TGGCCA               TGG^CCA
NarI         2301  GGCGCC               GG^CGCC
PflFI         798  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I      845  AACGTT               AA^CGTT
PstI         1486  CTGCAG               CTGCA^G
RsrII          20  CGGACCG              CG^G[AT]CCG
SacI          622  GAGCTC               GAGCT^C
SfoI         2301  GGCGCC               GGC^GCC
SmaI           87  CCCGGG               CCC^GGG
SphI         1060  GCATGC               GCATG^C
SwaI         1436  ATTTAAAT             ATTT^AAAT
Tth111I       798  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI          87  CCCGGG               C^CCGGG
XmaI           87  CCCGGG               C^CCGGG
XmnI          106  GAAGCGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Alw44I        995  GTGCAC               G^TGCAC
             1327  GTGCAC              
AlwNI        1633  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2153  CAGCCACTG           
AocI          100  CCTCAGG              CC^T[ACGT]AGG
              417  CCTCAGG             
ApaLI         995  GTGCAC               G^TGCAC
             1327  GTGCAC              
AvaI           87  CCCGGG               C^[CT]CG[GA]G
              698  CCCGAG              
BanII         622  GAGCTC               G[GA]GC[CT]^C
              685  GGGCCC              
BclI         1640  TGATCA               T^GATCA
             2066  TGATCA              
BglII        1163  AGATCT               A^GATCT
             1874  AGATCT              
BsmFI         442  GTCCC                GGGAC(10/14)
             1038  GTCCC               
BsoBI          87  CCCGGG               C^[CT]CG[GA]G
              698  CCCGAG              
BspHI        1310  TCATGA               T^CATGA
             1650  TCATGA              
BsrBI        2323  CCGCTC               CCGCTC(-3/-3)
               17  GAGCGG              
BstXI        1472  CCAACTGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2156  CCACTGACCTGG        
Bsu36I        100  CCTCAGG              CC^T[ACGT]AGG
              417  CCTCAGG             
DsaI          734  CCATGG               C^C[GA][CT]GG
             1884  CCATGG              
Eco81I        100  CCTCAGG              CC^T[ACGT]AGG
              417  CCTCAGG             
FbaI         1640  TGATCA               T^GATCA
             2066  TGATCA              
HgaI           82  GACGC                GACGC(5/10)
             1899  GCGTC               
NcoI          734  CCATGG               C^CATGG
             1884  CCATGG              
NspI         1060  GCATGC               [GA]CATG^[CT]
             2313  ACATGT              
PpuMI         569  AGGACCC              [GA]G^G[AT]CC[CT]
             1255  AGGTCCT             
RcaI         1310  TCATGA               T^CATGA
             1650  TCATGA              
SfcI          180  CTACAG               C^T[GA][CT]AG
             1486  CTGCAG              
SmlI          594  CTTAAG               C^T[CT][GA]AG
             1288  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        ApoI        AspHI       AvaII       
BanI        BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    Bsp1407I    BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PshAI       PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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