Restriction map for pF1KB3481
 (Linear) (Six-base) MAPSORT of: KIBB3481.seq  Check: 7705  from: 1  to: 2346



KIBB3481.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     123    2346 
   Size:        123    2223
 
AclI AA'CG_TT

Cuts at:      0     846    2346 
   Size:        846    1500
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     626    2346 
   Size:        220     406    1720

  Fragments arranged by size: 

                1720     406     220
 
AfeI AGC'GCT

Cuts at:      0    2335    2346 
   Size:       2335      11
 
AflII C'TTAA_G

Cuts at:      0     594    2346 
   Size:        594    1752
 
AflIII A'CryG_T

Cuts at:      0    2313    2346 
   Size:       2313      33
 
AleI CACnn'nnGTG

Cuts at:      0     154    1369    2346 
   Size:        154    1215     977

  Fragments arranged by size: 

                1215     977     154
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1638    2158    2346 
   Size:       1638     520     188

  Fragments arranged by size: 

                1638     520     188
 
ApaI G_GGCC'C

Cuts at:      0     689    2346 
   Size:        689    1657
 
ApaLI G'TGCA_C

Cuts at:      0     995    1327    2346 
   Size:        995     332    1019

  Fragments arranged by size: 

                1019     995     332
 
ApoI r'AATT_y

Cuts at:      0    1440    1549    1618    1772    1782    2194    2346 
   Size:       1440     109      69     154      10     412     152

  Fragments arranged by size: 

                1440     412     154     152     109      69      10
 
AvaI C'yCGr_G

Cuts at:      0      87     698    2346 
   Size:         87     611    1648

  Fragments arranged by size: 

                1648     611      87
 
BanI G'GyrC_C

Cuts at:      0     148    1532    2233    2301    2346 
   Size:        148    1384     701      68      45

  Fragments arranged by size: 

                1384     701     148      68      45
 
BanII G_rGCy'C

Cuts at:      0     626     689    2346 
   Size:        626      63    1657

  Fragments arranged by size: 

                1657     626      63
 
BbeI G_GCGC'C

Cuts at:      0    2305    2346 
   Size:       2305      41
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2102    2346 
   Size:       2102     244
 
BbvCI CC'TCA_GC

Cuts at:      0    1523    2346 
   Size:       1523     823
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1998    2346 
   Size:       1998     348
 
BclI T'GATC_A

Cuts at:      0    1640    2066    2346 
   Size:       1640     426     280

  Fragments arranged by size: 

                1640     426     280
 
BglII A'GATC_T

Cuts at:      0    1163    1874    2346 
   Size:       1163     711     472

  Fragments arranged by size: 

                1163     711     472
 
BlpI GC'TnA_GC

Cuts at:      0     427    2346 
   Size:        427    1919
 
Bme1580I G_kGCm'C

Cuts at:      0     689     999    1331    1537    2346 
   Size:        689     310     332     206     809

  Fragments arranged by size: 

                 809     689     332     310     206
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1122    2346 
   Size:       1122    1224
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2301    2346 
   Size:       2301      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     254     458     550     823     880    1762    1878 
   Size:        201      53     204      92     273      57     882     116

Cuts at:   1878    2187    2346 
   Size:        309     159

  Fragments arranged by size: 

                 882     309     273     204     201     159     116      92
                  57      53
 
Bpu10I CC'TnA_GC

Cuts at:      0    1457    1523    2346 
   Size:       1457      66     823

  Fragments arranged by size: 

                1457     823      66
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1273    2346 
   Size:       1273    1073
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       5    2346 
   Size:          5    2341
 
BsaHI Gr'CG_yC

Cuts at:      0      83    1899    2302    2346 
   Size:         83    1816     403      44

  Fragments arranged by size: 

                1816     403      83      44
 
BsaWI w'CCGG_w

Cuts at:      0     890    2346 
   Size:        890    1456
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     199     229    2346 
   Size:        199      30    2117

  Fragments arranged by size: 

                2117     199      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2104    2188    2191    2346 
   Size:       2104      84       3     155

  Fragments arranged by size: 

                2104     155      84       3
 
BseYI C'CCAG_C

Cuts at:      0    1089    1236    1389    2188    2305    2346 
   Size:       1089     147     153     799     117      41

  Fragments arranged by size: 

                1089     799     153     147     117      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1393    2346 
   Size:       1393     953
 
BsiHKAI G_wGCw'C

Cuts at:      0     626     999    1331    1364    2346 
   Size:        626     373     332      33     982

  Fragments arranged by size: 

                 982     626     373     332      33
 
BsmI GAATG_Cn'

Cuts at:      0     592    2346 
   Size:        592    1754
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1757    2346 
   Size:       1757     589
 
Bsp1286I G_dGCh'C

Cuts at:      0     626     689     999    1331    1364    1537    2346 
   Size:        626      63     310     332      33     173     809

  Fragments arranged by size: 

                 809     626     332     310     173      63      33
 
BspEI T'CCGG_A

Cuts at:      0     890    2346 
   Size:        890    1456
 
BspHI T'CATG_A

Cuts at:      0    1310    1650    2346 
   Size:       1310     340     696

  Fragments arranged by size: 

                1310     696     340
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     123    2346 
   Size:        123    2223
 
BsrBI CCG'CTC

Cuts at:      0      19    2325    2346 
   Size:         19    2306      21

  Fragments arranged by size: 

                2306      21      19
 
BsrFI r'CCGG_y

Cuts at:      0    1169    2346 
   Size:       1169    1177
 
BsrGI T'GTAC_A

Cuts at:      0    1598    2270    2316    2346 
   Size:       1598     672      46      30

  Fragments arranged by size: 

                1598     672      46      30
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1479    2163    2346 
   Size:       1479     684     183

  Fragments arranged by size: 

                1479     684     183
 
BstYI r'GATC_y

Cuts at:      0     708    1163    1874    2206    2346 
   Size:        708     455     711     332     140

  Fragments arranged by size: 

                 711     708     455     332     140
 
Bsu36I CC'TnA_GG

Cuts at:      0     101     418    2346 
   Size:        101     317    1928

  Fragments arranged by size: 

                1928     317     101
 
BtgI C'CryG_G

Cuts at:      0     734    1884    2346 
   Size:        734    1150     462

  Fragments arranged by size: 

                1150     734     462
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1488    2273    2346 
   Size:       1488     785      73

  Fragments arranged by size: 

                1488     785      73
 
BtsI GCAGTG_nn'

Cuts at:      0     684    1123    2346 
   Size:        684     439    1223

  Fragments arranged by size: 

                1223     684     439
 
DraI TTT'AAA

Cuts at:      0    1439    2346 
   Size:       1439     907
 
EaeI y'GGCC_r

Cuts at:      0     243    2346 
   Size:        243    2103
 
EarI CTCTTCn'nnn_

Cuts at:      0      59      71     782     958    1952    2114    2332    2346 
   Size:         59      12     711     176     994     162     218      14

  Fragments arranged by size: 

                 994     711     218     176     162      59      14      12
            
 
EcoICRI GAG'CTC

Cuts at:      0     624    2346 
   Size:        624    1722
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     220     254     458     550     626     823     880 
   Size:        201      19      34     204      92      76     197      57

Cuts at:    880    1762    1878    2187    2346 
   Size:        882     116     309     159

  Fragments arranged by size: 

                 882     309     204     201     197     159     116      92
                  76      57      34      19
 
EcoO109I rG'GnC_Cy

Cuts at:      0     221     570     686    1256    1305    2346 
   Size:        221     349     116     570      49    1041

  Fragments arranged by size: 

                1041     570     349     221     116      49
 
EcoRV GAT'ATC

Cuts at:      0     915    2346 
   Size:        915    1431
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     522    2346 
   Size:        522    1824
 
HaeII r_GCGC'y

Cuts at:      0    2229    2305    2337    2346 
   Size:       2229      76      32       9

  Fragments arranged by size: 

                2229      76      32       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     199     231    2346 
   Size:        199      32    2115

  Fragments arranged by size: 

                2115     199      32
 
HincII GTy'rAC

Cuts at:      0     906    2346 
   Size:        906    1440
 
KasI G'GCGC_C

Cuts at:      0    2301    2346 
   Size:       2301      45
 
MscI TGG'CCA

Cuts at:      0     245    2346 
   Size:        245    2101
 
MslI CAynn'nnrTG

Cuts at:      0     154     338     545     733    1369    2346 
   Size:        154     184     207     188     636     977

  Fragments arranged by size: 

                 977     636     207     188     184     154
 
MspA1I CmG'CkG

Cuts at:      0     198     376     940    1102    2346 
   Size:        198     178     564     162    1244

  Fragments arranged by size: 

                1244     564     198     178     162
 
NarI GG'CG_CC

Cuts at:      0    2302    2346 
   Size:       2302      44
 
NcoI C'CATG_G

Cuts at:      0     734    1884    2346 
   Size:        734    1150     462

  Fragments arranged by size: 

                1150     734     462
 
NspI r_CATG'y

Cuts at:      0    1064    2317    2346 
   Size:       1064    1253      29

  Fragments arranged by size: 

                1253    1064      29
 
PciI A'CATG_T

Cuts at:      0    2313    2346 
   Size:       2313      33
 
PfoI T'CCnGG_A

Cuts at:      0      40     566    1316    2346 
   Size:         40     526     750    1030

  Fragments arranged by size: 

                1030     750     526      40
 
PpuMI rG'GwC_Cy

Cuts at:      0     570    1256    2346 
   Size:        570     686    1090

  Fragments arranged by size: 

                1090     686     570
 
PshAI GACnn'nnGTC

Cuts at:      0     439    1408    1749    2346 
   Size:        439     969     341     597

  Fragments arranged by size: 

                 969     597     439     341
 
PspOMI G'GGCC_C

Cuts at:      0     685    2346 
   Size:        685    1661
 
PstI C_TGCA'G

Cuts at:      0    1490    2346 
   Size:       1490     856
 
PvuII CAG'CTG

Cuts at:      0     376     940    1102    2346 
   Size:        376     564     162    1244

  Fragments arranged by size: 

                1244     564     376     162
 
RsrII CG'GwC_CG

Cuts at:      0      21    2346 
   Size:         21    2325
 
SacI G_AGCT'C

Cuts at:      0     626    2346 
   Size:        626    1720
 
SapI GCTCTTCn'nnn_

Cuts at:      0     958    2114    2332    2346 
   Size:        958    1156     218      14

  Fragments arranged by size: 

                1156     958     218      14
 
SfcI C'TryA_G

Cuts at:      0     180    1486    2346 
   Size:        180    1306     860

  Fragments arranged by size: 

                1306     860     180
 
SfoI GGC'GCC

Cuts at:      0    2303    2346 
   Size:       2303      43
 
SmaI CCC'GGG

Cuts at:      0      89    2346 
   Size:         89    2257
 
SmlI C'TyrA_G

Cuts at:      0     594    1288    2346 
   Size:        594     694    1058

  Fragments arranged by size: 

                1058     694     594
 
SphI G_CATG'C

Cuts at:      0    1064    2346 
   Size:       1064    1282
 
StyI C'CwwG_G

Cuts at:      0     173     734    1187    1823    1884    2346 
   Size:        173     561     453     636      61     462

  Fragments arranged by size: 

                 636     561     462     453     173      61
 
SwaI ATTT'AAAT

Cuts at:      0    1439    2346 
   Size:       1439     907
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      29     219    2346 
   Size:         29     190    2127

  Fragments arranged by size: 

                2127     190      29
 
TatI w'GTAC_w

Cuts at:      0     344     475    1598    1961    2270    2316    2346 
   Size:        344     131    1123     363     309      46      30

  Fragments arranged by size: 

                1123     363     344     309     131      46      30
 
Tth111I GACn'n_nGTC

Cuts at:      0     801    2346 
   Size:        801    1545
 
XmaI C'CCGG_G

Cuts at:      0      87    2346 
   Size:         87    2259
 
XmnI GAAnn'nnTTC

Cuts at:      0     110    2346 
   Size:        110    2236
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AfeI    AflII   AflIII     AleI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRV     FalI    HaeII    Hin4I   HincII     KasI     MscI     MslI
   MspA1I     NarI     NcoI     NspI     PciI     PfoI    PpuMI    PshAI
   PspOMI     PstI    PvuII    RsrII     SacI     SapI     SfcI     SfoI
     SmaI     SmlI     SphI     StyI     SwaI    TaqII     TatI  Tth111I
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AgeI     AhdI     AlfI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BfrBI     BglI
    BmgBI     BmtI    BsaAI    BsaBI    BsiEI    BsiWI    BsrDI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I     ClaI    CspCI   DraIII
     DrdI     EagI     EciI    EcoNI    EcoRI     FseI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     StuI     TstI     XbaI     XcmI
     XhoI     ZraI
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