Restriction map for
pF1KB3419
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BciVI
BclI BcnI BfrI BlnI BlpI
Bpu1102I BsaAI BsaHI BseAI BsgI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I EcoRI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AclI 412 AACGTT AA^CGTT
AfaI 436 GTAC GT^AC
AhdI 397 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1533 GTGCAC G^TGCAC
AlwNI 1343 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1615 CCTCAGG CC^T[ACGT]AGG
ApaLI 1533 GTGCAC G^TGCAC
AspEI 397 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1533 GTGCAC G[AT]GC[AT]^C
BalI 1818 TGGCCA TGG^CCA
BcgI 1695 CGACTCAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2328 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 443 AGATCT A^GATCT
BsaBI 307 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 395 GAGACC GGTCTC(1/5)
BsaWI 2318 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1533 GTGCAC G[AT]GC[AT]^C
BspMI 1688 GCAGGT ACCTGC(4/8)
BssSI 798 CACGAG CACGAG(-5/-1)
BstEII 1793 GGTCACC G^GT[ACGT]ACC
BstPI 1793 GGTCACC G^GT[ACGT]ACC
BstXI 198 CCAGTTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 443 AGATCT [GA]^GATC[CT]
Bsu36I 1615 CCTCAGG CC^T[ACGT]AGG
Cac8I 1093 GCAAGC GC[ACGT]^[ACGT]GC
Eam1105I 397 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1615 CCTCAGG CC^T[ACGT]AGG
EcoNI 2113 CCTTCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1793 GGTCACC G^GT[ACGT]ACC
EcoRV 105 GATATC GAT^ATC
EcoT22I 352 ATGCAT ATGCA^T
HapII 2319 CCGG C^CGG
HindIII 1095 AAGCTT A^AGCTT
HpaII 2319 CCGG C^CGG
MamI 307 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 443 AGATCT [GA]^GATC[CT]
MluNI 1818 TGGCCA TGG^CCA
MscI 1818 TGGCCA TGG^CCA
MslI 1810 CACTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 985 CCGCTG C[AC]G^C[GT]G
MspI 2319 CCGG C^CGG
NcoI 511 CCATGG C^CATGG
NsiI 352 ATGCAT ATGCA^T
PflMI 1342 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 412 AACGTT AA^CGTT
RsaI 436 GTAC GT^AC
ScaI 435 AGTACT AGT^ACT
SfcI 2142 CTACAG C^T[GA][CT]AG
Van91I 1342 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 443 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 34 AAATTT [GA]^AATT[CT]
1236 AAATTT
AflIII 488 ACATGT A^C[GA][CT]GT
1581 ACATGT
AlwI 262 GGATC GGATC(4/5)
312 GGATC
ApoI 34 AAATTT [GA]^AATT[CT]
1236 AAATTT
BbsI 1021 GAAGAC GAAGAC(2/6)
1150 GAAGAC
BmyI 1533 GTGCAC G[AGT]GC[ACT]^C
1858 GTGCCC
BpuAI 1021 GAAGAC GAAGAC(2/6)
1150 GAAGAC
BseRI 139 CTCCTC GAGGAG(10/8)
298 GAGGAG
BsmI 350 GAATGC GAATGC(1/-1)
2103 GCATTC
Bsp1286I 1533 GTGCAC G[AGT]GC[ACT]^C
1858 GTGCCC
BspLU11I 488 ACATGT A^CATGT
1581 ACATGT
DraII 608 AGGTCCC [GA]G^G[ACGT]CC[CT]
2204 GGGACCT
DrdI 966 GACAGAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2278 GACAGCAAAGTC
DsaI 511 CCATGG C^C[GA][CT]GG
2382 CCGTGG
EaeI 748 CGGCCA [CT]^GGCC[GA]
1818 TGGCCA
EcoO109I 608 AGGTCCC [GA]G^G[ACGT]CC[CT]
2204 GGGACCT
FauI 984 CCCGC CCCGC(4/6)
2344 CCCGC
NdeI 364 CATATG CA^TATG
1544 CATATG
NspI 488 ACATGT [GA]CATG^[CT]
1581 ACATGT
PpuMI 608 AGGTCCC [GA]G^G[AT]CC[CT]
2204 GGGACCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BfaI BpmI BsaJI BsiYI BslI
BsmAI BsmBI BsmFI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I