Restriction map for
pF1KB3419
(Circular) (Six-base) MAPSORT of: pF1KB3419.seq Check: 9684 from: 1 to: 5513
pF1KB3419 5513 bp
With 182 enzymes: SgfI *
July 1, 2014 11:00 ..
AccI GT'mk_AC
Cuts at: 2551 2551
Size: 5513
Acc65I G'GTAC_C
Cuts at: 2529 2529
Size: 5513
AclI AA'CG_TT
Cuts at: 521 5353 521
Size: 4832 681
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1163 1412 2093 3338 3770 4123 4785 568
Size: 595 249 681 1245 432 353 662 1296
Fragments arranged by size:
1296 1245 681 662 595 432 353 249
AflIII A'CryG_T
Cuts at: 596 1689 2865 4665 4946 596
Size: 1093 1176 1800 281 1163
Fragments arranged by size:
1800 1176 1163 1093 281
AhdI GACnn_n'nnGTC
Cuts at: 510 510
Size: 5513
AlwNI CAG_nnn'CTG
Cuts at: 1456 2818 4256 1456
Size: 1362 1438 2713
Fragments arranged by size:
2713 1438 1362
ApaLI G'TGCA_C
Cuts at: 1641 4351 1641
Size: 2710 2803
ApoI r'AATT_y
Cuts at: 142 1344 2517 4866 5122 142
Size: 1202 1173 2349 256 533
Fragments arranged by size:
2349 1202 1173 533 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5513
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5513
AvaI C'yCGr_G
Cuts at: 2533 2533
Size: 5513
BamHI G'GATC_C
Cuts at: 2538 2538
Size: 5513
BanI G'GyrC_C
Cuts at: 121 565 1965 2529 2943 3191 3226 121
Size: 444 1400 564 414 248 35 2408
Fragments arranged by size:
2408 1400 564 444 414 248 35
BanII G_rGCy'C
Cuts at: 2527 3557 2527
Size: 1030 4483
BbeI G_GCGC'C
Cuts at: 2947 3195 2947
Size: 248 5265
BbsI GAAGACnn'nnnn_
Cuts at: 1136 1265 1136
Size: 129 5384
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1792 1826 1792
Size: 34 5479
BciVI GTATCCnnnnn_n'
Cuts at: 3411 4467 5513 3411
Size: 1056 1046 3411
Fragments arranged by size:
3411 1056 1046
BclI T'GATC_A
Cuts at: 3033 3033
Size: 5513
BfrBI ATG'CAT
Cuts at: 462 462
Size: 5513
BglI GCCn_nnn'nGGC
Cuts at: 2442 2442
Size: 5513
BglII A'GATC_T
Cuts at: 551 3028 5065 551
Size: 2477 2037 999
Fragments arranged by size:
2477 2037 999
BlpI GC'TnA_GC
Cuts at: 2625 2625
Size: 5513
Bme1580I G_kGCm'C
Cuts at: 1645 1970 3138 3231 4355 1645
Size: 325 1168 93 1124 2803
Fragments arranged by size:
2803 1168 1124 325 93
BmgBI CAC'GTC
Cuts at: 2155 2155
Size: 5513
BmrI ACTGGGnnnn_n'
Cuts at: 2725 2806 3141 5285 2725
Size: 81 335 2144 2953
Fragments arranged by size:
2953 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2382 3880 2382
Size: 1498 4015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 907 1270 614
Size: 293 363 4857
Fragments arranged by size:
4857 363 293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 1000 1791 2352 2689 3568 4282 4580 493
Size: 507 791 561 337 879 714 298 1426
Fragments arranged by size:
1426 879 791 714 561 507 337 298
BsaI GGTCTCn'nnnn_
Cuts at: 497 5217 497
Size: 4720 793
BsaAI yAC'GTr
Cuts at: 2866 2866
Size: 5513
BsaBI GATnn'nnATC
Cuts at: 6 419 3027 5064 6
Size: 413 2608 2037 455
Fragments arranged by size:
2608 2037 455 413
BsaHI Gr'CG_yC
Cuts at: 2944 3192 3894 5390 2944
Size: 248 702 1496 3067
Fragments arranged by size:
3067 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2426 2702 3223 3901 4312 4459 4733 2426
Size: 276 521 678 411 147 274 3206
Fragments arranged by size:
3206 678 521 411 276 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 880 910 943 973 880
Size: 30 33 30 5420
Fragments arranged by size:
5420 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 421 238
Size: 183 5330
BseYI C'CCAG_C
Cuts at: 2938 3696 4361 4769 2938
Size: 758 665 408 3682
Fragments arranged by size:
3682 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3055 3101 4331 104
Size: 2951 46 1230 1286
Fragments arranged by size:
2951 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1645 2527 3305 3495 4355 1645
Size: 882 778 190 860 2803
Fragments arranged by size:
2803 882 860 778 190
BsmI GAATG_Cn'
Cuts at: 464 2211 464
Size: 1747 3766
BsmBI CGTCTCn'nnnn_
Cuts at: 119 1947 2021 119
Size: 1828 74 3611
Fragments arranged by size:
3611 1828 74
Bsp1286I G_dGCh'C
Cuts at: 1645 1970 2527 3138 3231 3305 3495 3557 4355
Size: 325 557 611 93 74 190 62 798
Cuts at: 4355 1645
Size: 2803
Fragments arranged by size:
2803 798 611 557 325 190 93 74
62
BspHI T'CATG_A
Cuts at: 3945 3945
Size: 5513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1787 2563 2777 3079 3460 1787
Size: 776 214 302 381 3840
Fragments arranged by size:
3840 776 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3805 3859 5343 5508 50
Size: 3755 54 1484 165 55
Fragments arranged by size:
3755 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3425 3425
Size: 5513
BsrFI r'CCGG_y
Cuts at: 3511 3692 3511
Size: 181 5332
BssSI C'ACGA_G
Cuts at: 906 3784 4492 906
Size: 2878 708 1927
Fragments arranged by size:
2878 1927 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2890 2890
Size: 5513
BstBI TT'CG_AA
Cuts at: 3874 3874
Size: 5513
BstEII G'GTnAC_C
Cuts at: 1901 1901
Size: 5513
BstXI CCAn_nnnn'nTGG
Cuts at: 313 313
Size: 5513
BstYI r'GATC_y
Cuts at: 1 551 2538 3005 3028 3363 3609 3915 3927
Size: 550 1987 467 23 335 246 306 12
Cuts at: 3927 4013 5065 1
Size: 86 1052 449
Fragments arranged by size:
1987 1052 550 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1724 1724
Size: 5513
BtgI C'CryG_G
Cuts at: 619 2490 619
Size: 1871 3642
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3463 3644 3807 3463
Size: 181 163 5169
Fragments arranged by size:
5169 181 163
BtsI GCAGTG_nn'
Cuts at: 2756 2756
Size: 5513
DraI TTT'AAA
Cuts at: 726 1052 1212 1571 2512 726
Size: 326 160 359 941 3727
Fragments arranged by size:
3727 941 359 326 160
DrdI GACnn_nn'nnGTC
Cuts at: 1080 2392 3219 4563 1080
Size: 1312 827 1344 2030
Fragments arranged by size:
2030 1344 1312 827
EaeI y'GGCC_r
Cuts at: 856 1926 3098 3272 3663 3690 5035 856
Size: 1070 1172 174 391 27 1345 1334
Fragments arranged by size:
1345 1334 1172 1070 391 174 27
EagI C'GGCC_G
Cuts at: 3098 3098
Size: 5513
EarI CTCTTCn'nnn_
Cuts at: 1182 2055 2112 2375 3536 3746 1182
Size: 873 57 263 1161 210 2949
Fragments arranged by size:
2949 1161 873 263 210 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 964 3721 4463 4609 5323 964
Size: 2757 742 146 714 1154
Fragments arranged by size:
2757 1154 742 714 146
EcoICRI GAG'CTC
Cuts at: 2525 2525
Size: 5513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 614 907 1163 1270 1412 2093 3338 3770
Size: 46 293 256 107 142 681 1245 432
Cuts at: 3770 4123 4785 568
Size: 353 662 1296
Fragments arranged by size:
1296 1245 681 662 432 353 293 256
142 107 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 2225
Size: 5513
EcoO109I rG'GnC_Cy
Cuts at: 717 2313 2652 717
Size: 1596 339 3578
Fragments arranged by size:
3578 1596 339
EcoRI G'AATT_C
Cuts at: 2517 2517
Size: 5513
EcoRV GAT'ATC
Cuts at: 215 215
Size: 5513
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3532 3532
Size: 5513
FspI TGC'GCA
Cuts at: 3294 3294
Size: 5513
HaeII r_GCGC'y
Cuts at: 2947 3195 4425 5196 2947
Size: 248 1230 771 3264
Fragments arranged by size:
3264 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 183 215 496 528 880 912 3355 3385 3387
Size: 32 281 32 352 32 2443 30 2
Cuts at: 3387 3417 183
Size: 30 2279
Fragments arranged by size:
2443 2279 352 281 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2552 2552
Size: 5513
HindIII A'AGCT_T
Cuts at: 1203 1203
Size: 5513
KasI G'GCGC_C
Cuts at: 2943 3191 2943
Size: 248 5265
KpnI G_GTAC'C
Cuts at: 2533 2533
Size: 5513
MluI A'CGCG_T
Cuts at: 4946 4946
Size: 5513
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 588 1109 1770 1826 1883 4272 4456 588
Size: 521 661 56 57 2389 184 1645
Fragments arranged by size:
2389 1645 661 521 184 57 56
MscI TGG'CCA
Cuts at: 1928 3274 1928
Size: 1346 4167
MslI CAynn'nnrTG
Cuts at: 1922 3502 3629 1922
Size: 1580 127 3806
Fragments arranged by size:
3806 1580 127
MspA1I CmG'CkG
Cuts at: 1095 2624 2938 3298 4082 4327 1095
Size: 1529 314 360 784 245 2281
Fragments arranged by size:
2281 1529 784 360 314 245
NaeI GCC'GGC
Cuts at: 3694 3694
Size: 5513
NarI GG'CG_CC
Cuts at: 2944 3192 2944
Size: 248 5265
NcoI C'CATG_G
Cuts at: 619 619
Size: 5513
NdeI CA'TA_TG
Cuts at: 473 1653 473
Size: 1180 4333
NgoMIV G'CCGG_C
Cuts at: 3692 3692
Size: 5513
NsiI A_TGCA'T
Cuts at: 464 464
Size: 5513
NspI r_CATG'y
Cuts at: 600 1693 2566 3066 4669 600
Size: 1093 873 500 1603 1444
Fragments arranged by size:
1603 1444 1093 873 500
PasI CC'CwG_GG
Cuts at: 2309 2309
Size: 5513
PciI A'CATG_T
Cuts at: 596 1689 4665 596
Size: 1093 2976 1444
Fragments arranged by size:
2976 1444 1093
PflMI CCAn_nnn'nTGG
Cuts at: 1456 1456
Size: 5513
PfoI T'CCnGG_A
Cuts at: 884 884
Size: 5513
PmeI GTTT'AAAC
Cuts at: 2512 2512
Size: 5513
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1707 1739 3959 3991 1707
Size: 32 2220 32 3229
Fragments arranged by size:
3229 2220 32 32
PpuMI rG'GwC_Cy
Cuts at: 717 2313 717
Size: 1596 3917
PstI C_TGCA'G
Cuts at: 2560 3245 2560
Size: 685 4828
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5513
PvuII CAG'CTG
Cuts at: 2938 3298 2938
Size: 360 5153
RsrII CG'GwC_CG
Cuts at: 3708 3708
Size: 5513
SacI G_AGCT'C
Cuts at: 2527 2527
Size: 5513
SalI G'TCGA_C
Cuts at: 2550 2550
Size: 5513
SapI GCTCTTCn'nnn_
Cuts at: 3536 3746 3536
Size: 210 5303
SbfI CC_TGCA'GG
Cuts at: 2560 2560
Size: 5513
ScaI AGT'ACT
Cuts at: 545 5030 545
Size: 4485 1028
SfcI C'TryA_G
Cuts at: 33 2250 2556 3241 4209 4400 33
Size: 2217 306 685 968 191 1146
Fragments arranged by size:
2217 1146 968 685 306 191
SfoI GGC'GCC
Cuts at: 2945 3193 2945
Size: 248 5265
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5513
SmaI CCC'GGG
Cuts at: 2535 2535
Size: 5513
SmlI C'TyrA_G
Cuts at: 508 979 1806 2367 2668 3583 4297 4559 508
Size: 471 827 561 301 915 714 262 1462
Fragments arranged by size:
1462 915 827 714 561 471 301 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5513
SphI G_CATG'C
Cuts at: 2566 3066 2566
Size: 500 5013
StyI C'CwwG_G
Cuts at: 602 619 1286 2647 3001 602
Size: 17 667 1361 354 3114
Fragments arranged by size:
3114 1361 667 354 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3096 3229 3688 3897 3096
Size: 133 459 209 4712
Fragments arranged by size:
4712 459 209 133
TatI w'GTAC_w
Cuts at: 543 5028 543
Size: 4485 1028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 897 929 943 975 1459 1491 5030 5062 897
Size: 32 14 32 484 32 3539 32 1348
Fragments arranged by size:
3539 1348 484 32 32 32 32 14
Tth111I GACn'n_nGTC
Cuts at: 3310 3310
Size: 5513
XbaI T'CTAG_A
Cuts at: 2544 2544
Size: 5513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3008 3008
Size: 5513
XmaI C'CCGG_G
Cuts at: 2533 2533
Size: 5513
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BmtI Bpu10I BsgI
BsiWI BspEI BsrGI BssHII BstZ17I ClaI CspCI DraIII
FseI FspAI HpaI MfeI NheI NotI NruI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI