Restriction map for pF1KB3414
 (Linear) (Six-base) MAPSORT of: KIBB3414.seq  Check: 8491  from: 1  to: 2628



KIBB3414.orf

 With 182 enzymes: SgfI * 

                        September 4, 2008 14:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     648    1078    2628 
   Size:        648     430    1550

  Fragments arranged by size: 

                1550     648     430
 
AccI GT'mk_AC

Cuts at:      0     174     445    1340    2628 
   Size:        174     271     895    1288

  Fragments arranged by size: 

                1288     895     271     174
 
Acc65I G'GTAC_C

Cuts at:      0    1142    2628 
   Size:       1142    1486
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     875    1340    1454    1669    1723    1747    2200    2564 
   Size:        875     465     114     215      54      24     453     364

Cuts at:   2564    2628 
   Size:         64

  Fragments arranged by size: 

                 875     465     453     364     215     114      64      54
                  24
 
AflIII A'CryG_T

Cuts at:      0    1095    2628 
   Size:       1095    1533
 
AhdI GACnn_n'nnGTC

Cuts at:      0     268    1740    2628 
   Size:        268    1472     888

  Fragments arranged by size: 

                1472     888     268
 
AleI CACnn'nnGTG

Cuts at:      0    1903    2628 
   Size:       1903     725
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1065    2628 
   Size:       1065    1563
 
AlwNI CAG_nnn'CTG

Cuts at:      0     720     963    1365    2139    2628 
   Size:        720     243     402     774     489

  Fragments arranged by size: 

                 774     720     489     402     243
 
ApoI r'AATT_y

Cuts at:      0     841    1301    1924    2094    2383    2628 
   Size:        841     460     623     170     289     245

  Fragments arranged by size: 

                 841     623     460     289     245     170
 
AseI AT'TA_AT

Cuts at:      0    1246    2628 
   Size:       1246    1382
 
AvaI C'yCGr_G

Cuts at:      0      19    2628 
   Size:         19    2609
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     680     713     943     976    2628 
   Size:        680      33     230      33    1652

  Fragments arranged by size: 

                1652     680     230      33      33
 
BanI G'GyrC_C

Cuts at:      0    1142    2628 
   Size:       1142    1486
 
BanII G_rGCy'C

Cuts at:      0    1196    2628 
   Size:       1196    1432
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      31    2566    2628 
   Size:         31    2535      62

  Fragments arranged by size: 

                2535      62      31
 
BbvCI CC'TCA_GC

Cuts at:      0     392    2628 
   Size:        392    2236
 
BclI T'GATC_A

Cuts at:      0       8    1467    2628 
   Size:          8    1459    1161

  Fragments arranged by size: 

                1459    1161       8
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1888    2628 
   Size:       1888     740
 
BglII A'GATC_T

Cuts at:      0    1805    2628 
   Size:       1805     823
 
Bme1580I G_kGCm'C

Cuts at:      0    2040    2628 
   Size:       2040     588
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     358    1190    1253    2628 
   Size:        358     832      63    1375

  Fragments arranged by size: 

                1375     832     358      63
 
BmtI G_CTAG'C

Cuts at:      0     577    2628 
   Size:        577    2051
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67      94    1407    2435    2628 
   Size:         67      27    1313    1028     193

  Fragments arranged by size: 

                1313    1028     193      67      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     392     920    2281    2628 
   Size:        392     528    1361     347

  Fragments arranged by size: 

                1361     528     392     347
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1915    2628 
   Size:       1915     713
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1381    2628 
   Size:       1381    1247
 
BsaBI GATnn'nnATC

Cuts at:      0    1628    2423    2628 
   Size:       1628     795     205

  Fragments arranged by size: 

                1628     795     205
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      18      48    1085    1115    2628 
   Size:         18      30    1037      30    1513

  Fragments arranged by size: 

                1513    1037      30      30      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1587    2189    2628 
   Size:       1587     602     439

  Fragments arranged by size: 

                1587     602     439
 
BseYI C'CCAG_C

Cuts at:      0    1412    1855    2628 
   Size:       1412     443     773

  Fragments arranged by size: 

                1412     773     443
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374    2628 
   Size:        374    2254
 
BsiEI CG_ry'CG

Cuts at:      0      42      86    2628 
   Size:         42      44    2542

  Fragments arranged by size: 

                2542      44      42
 
BsiHKAI G_wGCw'C

Cuts at:      0     434     926     954    1711    2628 
   Size:        434     492      28     757     917

  Fragments arranged by size: 

                 917     757     492     434      28
 
BsmI GAATG_Cn'

Cuts at:      0     591    2628 
   Size:        591    2037
 
Bsp1286I G_dGCh'C

Cuts at:      0     434     926     954    1196    1711    2040    2628 
   Size:        434     492      28     242     515     329     588

  Fragments arranged by size: 

                 588     515     492     434     329     242      28
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     104     648    1078    2628 
   Size:        104     544     430    1550

  Fragments arranged by size: 

                1550     544     430     104
 
BsrDI GCAATG_nn'

Cuts at:      0     806    2408    2628 
   Size:        806    1602     220

  Fragments arranged by size: 

                1602     806     220
 
BsrFI r'CCGG_y

Cuts at:      0     311    2628 
   Size:        311    2317
 
BsrGI T'GTAC_A

Cuts at:      0    2476    2628 
   Size:       2476     152
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1459    2628 
   Size:       1459    1169
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     129    2413    2628 
   Size:        129    2284     215

  Fragments arranged by size: 

                2284     215     129
 
BstYI r'GATC_y

Cuts at:      0     204     359    1805    2628 
   Size:        204     155    1446     823

  Fragments arranged by size: 

                1446     823     204     155
 
Bsu36I CC'TnA_GG

Cuts at:      0    1570    2628 
   Size:       1570    1058
 
BtgI C'CryG_G

Cuts at:      0      86    2628 
   Size:         86    2542
 
BtsI GCAGTG_nn'

Cuts at:      0    1163    2628 
   Size:       1163    1465
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      90     125    2628 
   Size:         90      35    2503

  Fragments arranged by size: 

                2503      90      35
 
DraI TTT'AAA

Cuts at:      0    1950    2184    2628 
   Size:       1950     234     444

  Fragments arranged by size: 

                1950     444     234
 
DraIII CAC_nnn'GTG

Cuts at:      0     334     680    2628 
   Size:        334     346    1948

  Fragments arranged by size: 

                1948     346     334
 
EaeI y'GGCC_r

Cuts at:      0      83     376     401     878    1064    2628 
   Size:         83     293      25     477     186    1564

  Fragments arranged by size: 

                1564     477     293     186      83      25
 
EagI C'GGCC_G

Cuts at:      0      83    2628 
   Size:         83    2545
 
EarI CTCTTCn'nnn_

Cuts at:      0     545    1766    2219    2628 
   Size:        545    1221     453     409

  Fragments arranged by size: 

                1221     545     453     409
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2565    2628 
   Size:       2565      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67      94     875    1340    1407    1454    1669    1723 
   Size:         67      27     781     465      67      47     215      54

Cuts at:   1723    1747    2200    2435    2564    2628 
   Size:         24     453     235     129      64

  Fragments arranged by size: 

                 781     465     453     235     215     129      67      67
                  64      54      47      27      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     769    2628 
   Size:        769    1859
 
EcoRI G'AATT_C

Cuts at:      0     841    1924    2628 
   Size:        841    1083     704

  Fragments arranged by size: 

                1083     841     704
 
EcoRV GAT'ATC

Cuts at:      0     213    2628 
   Size:        213    2415
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      18      50     254     286     619     651    1359    1391 
   Size:         18      32     204      32     333      32     708      32

Cuts at:   1391    1726    1758    2628 
   Size:        335      32     870

  Fragments arranged by size: 

                 870     708     335     333     204      32      32      32
                  32      32      18
 
HincII GTy'rAC

Cuts at:      0     446    2540    2628 
   Size:        446    2094      88

  Fragments arranged by size: 

                2094     446      88
 
HindIII A'AGCT_T

Cuts at:      0     887    2628 
   Size:        887    1741
 
HpaI GTT'AAC

Cuts at:      0    2540    2628 
   Size:       2540      88
 
KpnI G_GTAC'C

Cuts at:      0    1146    2628 
   Size:       1146    1482
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2072    2628 
   Size:       2072     556
 
MscI TGG'CCA

Cuts at:      0     378     403     880    1066    2628 
   Size:        378      25     477     186    1562

  Fragments arranged by size: 

                1562     477     378     186      25
 
MslI CAynn'nnrTG

Cuts at:      0    1793    1903    2354    2432    2628 
   Size:       1793     110     451      78     196

  Fragments arranged by size: 

                1793     451     196     110      78
 
MspA1I CmG'CkG

Cuts at:      0     169     396    1282    1855    2008    2082    2628 
   Size:        169     227     886     573     153      74     546

  Fragments arranged by size: 

                 886     573     546     227     169     153      74
 
NdeI CA'TA_TG

Cuts at:      0    1584    2628 
   Size:       1584    1044
 
NheI G'CTAG_C

Cuts at:      0     573    2628 
   Size:        573    2055
 
NspI r_CATG'y

Cuts at:      0    1099    2628 
   Size:       1099    1529
 
PciI A'CATG_T

Cuts at:      0    1095    2628 
   Size:       1095    1533
 
PfoI T'CCnGG_A

Cuts at:      0      71     138    2628 
   Size:         71      67    2490

  Fragments arranged by size: 

                2490      71      67
 
PpuMI rG'GwC_Cy

Cuts at:      0     769    2628 
   Size:        769    1859
 
PshAI GACnn'nnGTC

Cuts at:      0    1654    2628 
   Size:       1654     974
 
PstI C_TGCA'G

Cuts at:      0    1455    2628 
   Size:       1455    1173
 
PvuI CG_AT'CG

Cuts at:      0      42    2628 
   Size:         42    2586
 
PvuII CAG'CTG

Cuts at:      0     169     396    1282    1855    2008    2082    2628 
   Size:        169     227     886     573     153      74     546

  Fragments arranged by size: 

                 886     573     546     227     169     153      74
 
SalI G'TCGA_C

Cuts at:      0     444    2628 
   Size:        444    2184
 
SapI GCTCTTCn'nnn_

Cuts at:      0     545    2628 
   Size:        545    2083
 
SexAI A'CCwGG_T

Cuts at:      0    1991    2628 
   Size:       1991     637
 
SfcI C'TryA_G

Cuts at:      0     955    1292    1357    1451    1538    2628 
   Size:        955     337      65      94      87    1090

  Fragments arranged by size: 

                1090     955     337      94      87      65
 
SmlI C'TyrA_G

Cuts at:      0      19    1930    2628 
   Size:         19    1911     698

  Fragments arranged by size: 

                1911     698      19
 
SpeI A'CTAG_T

Cuts at:      0    1212    1902    2628 
   Size:       1212     690     726

  Fragments arranged by size: 

                1212     726     690
 
StuI AGG'CCT

Cuts at:      0    1945    2224    2628 
   Size:       1945     279     404

  Fragments arranged by size: 

                1945     404     279
 
StyI C'CwwG_G

Cuts at:      0    1619    2628 
   Size:       1619    1009
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1906    2628 
   Size:       1906     722
 
TatI w'GTAC_w

Cuts at:      0     749    1869    1934    2476    2496    2628 
   Size:        749    1120      65     542      20     132

  Fragments arranged by size: 

                1120     749     542     132      65      20
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     666     698    1085    1117    2628 
   Size:        666      32     387      32    1511

  Fragments arranged by size: 

                1511     666     387      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      30    1087    2628 
   Size:         30    1057    1541

  Fragments arranged by size: 

                1541    1057      30
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     108     371    1852    2628 
   Size:        108     263    1481     776

  Fragments arranged by size: 

                1481     776     263     108
 
XhoI C'TCGA_G

Cuts at:      0      19    2628 
   Size:         19    2609
 
XmnI GAAnn'nnTTC

Cuts at:      0     386    2387    2628 
   Size:        386    2001     241

  Fragments arranged by size: 

                2001     386     241
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AhdI     AleI     AlfI
    AlwNI     ApoI     AseI     AvaI     BaeI     BanI    BanII     BbsI
    BbvCI     BclI     BglI    BglII Bme1580I     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI     DraI
   DraIII     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI
    EcoRV    Hin4I   HincII  HindIII     HpaI     KpnI     MmeI     MscI
     MslI   MspA1I     NdeI     NheI     NspI     PciI     PfoI    PpuMI
    PshAI     PstI     PvuI    PvuII     SalI     SapI    SexAI     SfcI
     SmlI     SpeI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AloI     ApaI    ApaLI
     AscI    AsiSI    AvrII    BamHI     BbeI     BcgI    BciVI    BfrBI
     BlpI    BmgBI     BplI    BsaAI    BsaHI    BsaWI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI   BssHII    BssSI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI     DrdI  EcoICRI    EcoNI     FalI     FseI     FspI
    FspAI    HaeII     KasI     MfeI     MluI     NaeI     NarI     NcoI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI    RsrII     SacI
    SacII    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     SspI     SwaI     XbaI     XmaI
     ZraI
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