Restriction map for pF1KB3343
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

Acc65I      AclI        AcsI        AflII       AflIII      
AgeI        Alw44I      Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AvrII       BamHI       BbeI        BbsI        BcgI        
BciVI       BclI        BfrI        BlnI        BpuAI       
BsaBI       BsgI        BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrDI       
BsrGI       BssSI       Bst1107I    BstBI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HaeII       HindIII     HpaI        KasI        KpnI        
KspI        MamI        MluI        MseI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
ScaI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        TaqI        Tru9I       TthHB8I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         671  GACGTC               GACGT^C
AccI          520  GTCTAC               GT^[AC][GT]AC
AccIII       2504  TCCGGA               T^CCGGA
AcyI          671  GACGTC               G[GA]^CG[CT]C
AocI         2359  CCTGAGG              CC^T[ACGT]AGG
AspI         2528  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1409  TGCGCA               TGC^GCA
BbrPI        1143  CACGTG               CAC^GTG
BbvCI         876  GCTGAGG              CCTCAGC(-5/-2)
BglI           31  GCCCTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1677  AGATCT               A^GATCT
BlpI          771  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      771  GCTCAGC              GC^T[ACGT]AGC
BsaHI         671  GACGTC               G[GA]^CG[CT]C
BsaWI        2504  TCCGGA               [AT]^CCGG[AT]
BseAI        2504  TCCGGA               T^CCGGA
BsmBI         960  CGTCTC               CGTCTC(1/5)
BspEI        2504  TCCGGA               T^CCGGA
BsrBI        1797  CCGCTC               CCGCTC(-3/-3)
BssHII       1291  GCGCGC               G^CGCGC
BstEII       2333  GGTCACC              G^GT[ACGT]ACC
BstPI        2333  GGTCACC              G^GT[ACGT]ACC
Bsu36I       2359  CCTGAGG              CC^T[ACGT]AGG
CelII         771  GCTCAGC              GC^T[ACGT]AGC
Eco81I       2359  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      2333  GGTCACC              G^GT[ACGT]ACC
FspI         1409  TGCGCA               TGC^GCA
HgaI          550  GACGC                GACGC(5/10)
Hin1I         671  GACGTC               G[GA]^CG[CT]C
MfeI         2312  CAATTG               C^AATTG
MroI         2504  TCCGGA               T^CCGGA
MunI         2312  CAATTG               C^AATTG
NcoI           26  CCATGG               C^CATGG
PflFI        2528  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1143  CACGTG               CAC^GTG
PmlI         1143  CACGTG               CAC^GTG
SapI         2616  GCTCTTC              GCTCTTC(1/4)
SbfI          131  CCTGCAGG             CCTGCA^GG
SfiI           30  GGCCCTGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         2523  CCCGGG               CCC^GGG
Sse8387I      131  CCTGCAGG             CCTGCA^GG
StuI          349  AGGCCT               AGG^CCT
Tsp509I      2313  AATT                 ^AATT
Tth111I      2528  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2523  CCCGGG               C^CCGGG
XmaI         2523  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII         939  CGCG                 CG^CG
             1292  CGCG                
AhdI         1303  GACACCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2641  GACTACCTGTC         
ApaI         1771  GGGCCC               GGGCC^C
             2485  GGGCCC              
AspEI        1303  GACACCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2641  GACTACCTGTC         
BalI          803  TGGCCA               TGG^CCA
             2243  TGGCCA              
BsaAI         622  TACGTG               [CT]AC^GT[GA]
             1143  CACGTG              
BsaI         1165  GAGACC               GGTCTC(1/5)
              813  GGTCTC              
BsrFI        1255  ACCGGC               [GA]^CCGG[CT]
             2321  GCCGGT              
BstAPI         90  GCAGACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              600  GCACCTCATGC         
BstUI         939  CGCG                 CG^CG
             1292  CGCG                
Cfr10I       1255  ACCGGC               [GA]^CCGG[CT]
             2321  GCCGGT              
Eam1105I     1303  GACACCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2641  GACTACCTGTC         
EarI         2617  CTCTTC               CTCTTC(1/4)
              859  GAAGAG              
EcoNI        1211  CCTCCACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2550  CCTAGACCAGG         
Ksp632I      2617  CTCTTC               CTCTTC(1/4)
              859  GAAGAG              
MluNI         803  TGGCCA               TGG^CCA
             2243  TGGCCA              
MscI          803  TGGCCA               TGG^CCA
             2243  TGGCCA              
MvnI          939  CGCG                 CG^CG
             1292  CGCG                
PvuII         774  CAGCTG               CAG^CTG
             1667  CAGCTG              
SexAI        1216  ACCAGGT              A^CC[AT]GGT
             2147  ACCTGGT             
TfiI          921  GATTC                G^A[AT]TC
             1583  GATTC               
XcmI           26  CCATGGCCCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              691  CCAGAGGTTTGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TseI        Tsp45I      
TspRI       Van91I      XhoII       
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