Restriction map for pF1KB3343
 (Linear) (Six-base) MAPSORT of: KIBB3343.seq  Check: 9880  from: 1  to: 2691



KIBB3343.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     125     139    1896    2691 
   Size:        125      14    1757     795

  Fragments arranged by size: 

                1757     795     125      14
 
AatII G_ACGT'C

Cuts at:      0     675    2691 
   Size:        675    2016
 
AccI GT'mk_AC

Cuts at:      0     521    2691 
   Size:        521    2170
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517    2650    2691 
   Size:        517    2133      41

  Fragments arranged by size: 

                2133     517      41
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1308    2646    2691 
   Size:       1308    1338      45

  Fragments arranged by size: 

                1338    1308      45
 
AleI CACnn'nnGTG

Cuts at:      0     622    2691 
   Size:        622    2069
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1210    2691 
   Size:       1210    1481
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1555    1587    2691 
   Size:       1555      32    1104

  Fragments arranged by size: 

                1555    1104      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18      96     741    1223    2272    2691 
   Size:         18      78     645     482    1049     419

  Fragments arranged by size: 

                1049     645     482     419      78      18
 
ApaI G_GGCC'C

Cuts at:      0    1775    2489    2691 
   Size:       1775     714     202

  Fragments arranged by size: 

                1775     714     202
 
AvaI C'yCGr_G

Cuts at:      0     629    2428    2523    2691 
   Size:        629    1799      95     168

  Fragments arranged by size: 

                1799     629     168      95
 
BanI G'GyrC_C

Cuts at:      0     358     908    2601    2691 
   Size:        358     550    1693      90

  Fragments arranged by size: 

                1693     550     358      90
 
BanII G_rGCy'C

Cuts at:      0     407     691     890    1003    1211    1775    1832    2095 
   Size:        407     284     199     113     208     564      57     263

Cuts at:   2095    2265    2306    2489    2691 
   Size:        170      41     183     202

  Fragments arranged by size: 

                 564     407     284     263     208     202     199     183
                 170     113      57      41
 
BbvCI CC'TCA_GC

Cuts at:      0     877    2691 
   Size:        877    1814
 
BglI GCCn_nnn'nGGC

Cuts at:      0      37    2691 
   Size:         37    2654
 
BglII A'GATC_T

Cuts at:      0    1677    2691 
   Size:       1677    1014
 
BlpI GC'TnA_GC

Cuts at:      0     772    2691 
   Size:        772    1919
 
Bme1580I G_kGCm'C

Cuts at:      0     278     361     932    1775    2489    2597    2606    2691 
   Size:        278      83     571     843     714     108       9      85

  Fragments arranged by size: 

                 843     714     571     278     108      85      83       9
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     865    1233    2691 
   Size:        865     368    1458

  Fragments arranged by size: 

                1458     865     368
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1311    2691 
   Size:       1311    1380
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     474     526     763    1156    1225    1293    1582 
   Size:        229     245      52     237     393      69      68     289

Cuts at:   1582    1649    1850    1897    2023    2135    2279    2691 
   Size:         67     201      47     126     112     144     412

  Fragments arranged by size: 

                 412     393     289     245     237     229     201     144
                 126     112      69      68      67      52      47
 
Bpu10I CC'TnA_GC

Cuts at:      0     401     877    1556    2691 
   Size:        401     476     679    1135

  Fragments arranged by size: 

                1135     679     476     401
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     819    1159    2691 
   Size:        819     340    1532

  Fragments arranged by size: 

                1532     819     340
 
BsaAI yAC'GTr

Cuts at:      0     624    1145    2691 
   Size:        624     521    1546

  Fragments arranged by size: 

                1546     624     521
 
BsaHI Gr'CG_yC

Cuts at:      0     672    2691 
   Size:        672    2019
 
BsaWI w'CCGG_w

Cuts at:      0    2504    2691 
   Size:       2504     187
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     202     232     847     877    1555    1585    1783    1813 
   Size:        202      30     615      30     678      30     198      30

Cuts at:   1813    1870    1900    2691 
   Size:         57      30     791

  Fragments arranged by size: 

                 791     678     615     202     198      57      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     553    1438    2127    2691 
   Size:        553     885     689     564

  Fragments arranged by size: 

                 885     689     564     553
 
BseYI C'CCAG_C

Cuts at:      0      47     158     383     610     676     701     710     728 
   Size:         47     111     225     227      66      25       9      18

Cuts at:    728     838    1070    1441    1699    1706    1897    2224    2691 
   Size:        110     232     371     258       7     191     327     467

  Fragments arranged by size: 

                 467     371     327     258     232     227     225     191
                 111     110      66      47      25      18       9       7
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     602     773     868    1003    1370    2095    2306    2691 
   Size:        602     171      95     135     367     725     211     385

  Fragments arranged by size: 

                 725     602     385     367     211     171     135      95
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     966    2691 
   Size:        966    1725
 
Bsp1286I G_dGCh'C

Cuts at:      0     278     361     407     602     691     773     868     890 
   Size:        278      83      46     195      89      82      95      22

Cuts at:    890     932    1003    1211    1370    1775    1832    2095    2265 
   Size:         42      71     208     159     405      57     263     170

Cuts at:   2265    2306    2489    2597    2606    2691 
   Size:         41     183     108       9      85

  Fragments arranged by size: 

                 405     278     263     208     195     183     170     159
                 108      95      89      85      83      82      71      57
                  46      42      41      22       9
 
BspEI T'CCGG_A

Cuts at:      0    2504    2691 
   Size:       2504     187
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     125     139     422    1896    2691 
   Size:        125      14     283    1474     795

  Fragments arranged by size: 

                1474     795     283     125      14
 
BsrBI CCG'CTC

Cuts at:      0    1799    2691 
   Size:       1799     892
 
BsrFI r'CCGG_y

Cuts at:      0    1255    2321    2691 
   Size:       1255    1066     370

  Fragments arranged by size: 

                1255    1066     370
 
BssHII G'CGCG_C

Cuts at:      0    1291    2691 
   Size:       1291    1400
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      96     606    2691 
   Size:         96     510    2085

  Fragments arranged by size: 

                2085     510      96
 
BstEII G'GTnAC_C

Cuts at:      0    2333    2691 
   Size:       2333     358
 
BstYI r'GATC_y

Cuts at:      0     501    1118    1268    1430    1677    2691 
   Size:        501     617     150     162     247    1014

  Fragments arranged by size: 

                1014     617     501     247     162     150
 
Bsu36I CC'TnA_GG

Cuts at:      0    2360    2691 
   Size:       2360     331
 
BtgI C'CryG_G

Cuts at:      0      26     945    1537    1952    2691 
   Size:         26     919     592     415     739

  Fragments arranged by size: 

                 919     739     592     415      26
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1344    1948    2691 
   Size:       1344     604     743

  Fragments arranged by size: 

                1344     743     604
 
BtsI GCAGTG_nn'

Cuts at:      0     912    2691 
   Size:        912    1779
 
EaeI y'GGCC_r

Cuts at:      0     803     948    1139    2243    2691 
   Size:        803     145     191    1104     448

  Fragments arranged by size: 

                1104     803     448     191     145
 
EarI CTCTTCn'nnn_

Cuts at:      0     854    2623    2691 
   Size:        854    1769      68

  Fragments arranged by size: 

                1769     854      68
 
EcoICRI GAG'CTC

Cuts at:      0    1001    2093    2304    2691 
   Size:       1001    1092     211     387

  Fragments arranged by size: 

                1092    1001     387     211
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     474     517     526     763    1156    1225    1293 
   Size:        229     245      43       9     237     393      69      68

Cuts at:   1293    1582    1649    1850    1897    2023    2135    2279    2650 
   Size:        289      67     201      47     126     112     144     371

Cuts at:   2650    2691 
   Size:         41

  Fragments arranged by size: 

                 393     371     289     245     237     229     201     144
                 126     112      69      68      67      47      43      41
                   9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1215    2554    2691 
   Size:       1215    1339     137

  Fragments arranged by size: 

                1339    1215     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0     201     411    1244    1620    1772    1871    2017    2109 
   Size:        201     210     833     376     152      99     146      92

Cuts at:   2109    2171    2269    2433    2485    2527    2691 
   Size:         62      98     164      52      42     164

  Fragments arranged by size: 

                 833     376     210     201     164     164     152     146
                  99      98      92      62      52      42
 
FspI TGC'GCA

Cuts at:      0    1411    2691 
   Size:       1411    1280
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     202     234     316     348    1008    1040    1294    1326 
   Size:        202      32      82      32     660      32     254      32

Cuts at:   1326    1630    1662    2308    2340    2632    2664    2691 
   Size:        304      32     646      32     292      32      27

  Fragments arranged by size: 

                 660     646     304     292     254     202      82      32
                  32      32      32      32      32      32      27
 
HincII GTy'rAC

Cuts at:      0     171     324    2670    2691 
   Size:        171     153    2346      21

  Fragments arranged by size: 

                2346     171     153      21
 
MfeI C'AATT_G

Cuts at:      0    2312    2691 
   Size:       2312     379
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     363     593    2691 
   Size:        363     230    2098

  Fragments arranged by size: 

                2098     363     230
 
MscI TGG'CCA

Cuts at:      0     805    2245    2691 
   Size:        805    1440     446

  Fragments arranged by size: 

                1440     805     446
 
MslI CAynn'nnrTG

Cuts at:      0     622    1015    1363    1634    2691 
   Size:        622     393     348     271    1057

  Fragments arranged by size: 

                1057     622     393     348     271
 
MspA1I CmG'CkG

Cuts at:      0      87     162     269     776    1669    2691 
   Size:         87      75     107     507     893    1022

  Fragments arranged by size: 

                1022     893     507     107      87      75
 
NcoI C'CATG_G

Cuts at:      0      26    2691 
   Size:         26    2665
 
NspI r_CATG'y

Cuts at:      0     243     391    1500    1633    2691 
   Size:        243     148    1109     133    1058

  Fragments arranged by size: 

                1109    1058     243     148     133
 
PasI CC'CwG_GG

Cuts at:      0      12     407     496     588    2386    2691 
   Size:         12     395      89      92    1798     305

  Fragments arranged by size: 

                1798     395     305      92      89      12
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     683     741    1188    1223    1648    1935    2691 
   Size:        683      58     447      35     425     287     756

  Fragments arranged by size: 

                 756     683     447     425     287      58      35
 
PfoI T'CCnGG_A

Cuts at:      0     206    2691 
   Size:        206    2485
 
PmlI CAC'GTG

Cuts at:      0    1145    2691 
   Size:       1145    1546
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1555    1587    2691 
   Size:       1555      32    1104

  Fragments arranged by size: 

                1555    1104      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     201     411    1244    1620    1871    2109    2527    2691 
   Size:        201     210     833     376     251     238     418     164

  Fragments arranged by size: 

                 833     418     376     251     238     210     201     164
            
 
PspOMI G'GGCC_C

Cuts at:      0    1771    2485    2691 
   Size:       1771     714     206

  Fragments arranged by size: 

                1771     714     206
 
PstI C_TGCA'G

Cuts at:      0      92     136     419    1989    2145    2450    2691 
   Size:         92      44     283    1570     156     305     241

  Fragments arranged by size: 

                1570     305     283     241     156      92      44
 
PvuII CAG'CTG

Cuts at:      0     776    1669    2691 
   Size:        776     893    1022

  Fragments arranged by size: 

                1022     893     776
 
SacI G_AGCT'C

Cuts at:      0    1003    2095    2306    2691 
   Size:       1003    1092     211     385

  Fragments arranged by size: 

                1092    1003     385     211
 
SanDI GG'GwC_CC

Cuts at:      0    1871    2527    2691 
   Size:       1871     656     164

  Fragments arranged by size: 

                1871     656     164
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2623    2691 
   Size:       2623      68
 
SbfI CC_TGCA'GG

Cuts at:      0     136    2691 
   Size:        136    2555
 
SexAI A'CCwGG_T

Cuts at:      0    1216    2147    2691 
   Size:       1216     931     544

  Fragments arranged by size: 

                1216     931     544
 
SfcI C'TryA_G

Cuts at:      0      88     132     415    1059    1577    1985    2141    2446 
   Size:         88      44     283     644     518     408     156     305

Cuts at:   2446    2691 
   Size:        245

  Fragments arranged by size: 

                 644     518     408     305     283     245     156      88
                  44
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      37    2691 
   Size:         37    2654
 
SmaI CCC'GGG

Cuts at:      0    2525    2691 
   Size:       2525     166
 
StuI AGG'CCT

Cuts at:      0     351    2691 
   Size:        351    2340
 
StyI C'CwwG_G

Cuts at:      0      26     465     973    1043    2041    2338    2659    2691 
   Size:         26     439     508      70     998     297     321      32

  Fragments arranged by size: 

                 998     508     439     321     297      70      32      26
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     163     801    1177    1317    2691 
   Size:        163     638     376     140    1374

  Fragments arranged by size: 

                1374     638     376     163     140
 
TatI w'GTAC_w

Cuts at:      0    1124    2691 
   Size:       1124    1567
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     475     507     847     879    1260    1292    1783    1815 
   Size:        475      32     340      32     381      32     491      32

Cuts at:   1815    1870    1902    1937    1969    2691 
   Size:         55      32      35      32     722

  Fragments arranged by size: 

                 722     491     475     381     340      55      35      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2531    2691 
   Size:       2531     160
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      33     698    2691 
   Size:         33     665    1993

  Fragments arranged by size: 

                1993     665      33
 
XmaI C'CCGG_G

Cuts at:      0    2523    2691 
   Size:       2523     168
 
ZraI GAC'GTC

Cuts at:      0     673    2691 
   Size:        673    2018
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AhdI     AleI     AlfI     AloI
    AlwNI     ApaI     AvaI     BanI    BanII    BbvCI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI   BssHII   BstAPI   BstEII    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FspI    Hin4I   HincII     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NspI     PasI    PflMI     PfoI     PmlI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     SapI
     SbfI    SexAI     SfcI     SfiI     SmaI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BmgBI     BmtI    BpuEI    BsaBI
     BsgI    BsiEI    BsiWI     BsmI    BspHI    BsrDI    BsrGI    BssSI
    BstBI    BstXI  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     ScaI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XhoI     XmnI
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